{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:11:30Z","timestamp":1785409890302,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"crossref","award":["CHI-24025"],"award-info":[{"award-number":["CHI-24025"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"crossref"}]},{"award":["CHI-24025"],"award-info":[{"award-number":["CHI-24025"]}],"id":[{"id":"https:\/\/ror.org\/00gtfax65","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Ampicillin residues in animal-derived foods may cause allergic reactions and promote antimicrobial resistance in consumers; however, data on residue behavior in poultry remain limited. This study aimed to evaluate the depletion of ampicillin in muscle and skin plus fat of broiler chickens. Thirty birds were treated with ampicillin intramuscularly (20 mg kg\u22121 every 24 h for three days) and sacrificed at 0.5, 1, 2, 5, and 9 days post-administration. Samples were analyzed by liquid chromatography coupled with tandem mass spectrometry, a method successfully validated according to Commission Implementing Regulation (EU) 2021\/808, VICH GL49 and GL2. Quantification was performed by linear regression from matrix-matched calibration curves. Residue depletion was evaluated following the European Medicines Agency guidelines. Ampicillin residues in muscle were detected only during the first 24 h post-administration (6.50\u20138.48 \u00b5g kg\u22121). Residues in skin plus fat remained detectable until day 5 post-administration (6.87\u201359.88 \u00b5g kg\u22121). Based on this, the withdrawal period calculated for skin plus fat was 9 days considering EU maximum residue limit (MRL) and 19 days considering method limit of quantification, with 95% confidence. These results provide critical data on ampicillin residue kinetics under controlled experimental conditions, supporting risk assessments and the establishment of MRLs in broiler chickens by the Codex Alimentarius.<\/jats:p>","DOI":"10.3390\/ani16121821","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:44:58Z","timestamp":1781484298000},"page":"1821","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ampicillin Depletion and Withdrawal Period in Broilers: Tissue Residue Analysis After Intramuscular Administration"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5557-8072","authenticated-orcid":false,"given":"Paula","family":"Cort\u00e9s","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"},{"name":"Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile, Santa Rosa 11315, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximiliano","family":"Castillo","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Codoceo Valenzuela","sequence":"additional","affiliation":[{"name":"Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Manr\u00edquez Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1496-014X","authenticated-orcid":false,"given":"Bel\u00e9n","family":"Pinto","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1562-955X","authenticated-orcid":false,"given":"Ekaterina","family":"Pokrant","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad San Sebasti\u00e1n, Bellavista 7, Recoleta, Santiago 8420524, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Maddaleno","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7239-3294","authenticated-orcid":false,"given":"Sebasti\u00e1n","family":"Zavala","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Flores","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5641-9562","authenticated-orcid":false,"given":"Javiera","family":"Cornejo","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pharmacology (FARMAVET), Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"},{"name":"Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"},{"name":"Laboratory of Food Safety (INOCUIVET), Department of Preventive Animal Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"key":"ref_1","unstructured":"Sumano, H., and Guti\u00e9rrez, L. 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