{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:53:39Z","timestamp":1785405219485,"version":"3.56.0"},"reference-count":44,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2023YFD1301200"],"award-info":[{"award-number":["2023YFD1301200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hunan Agricultural University Institutional Project","award":["25SF050"],"award-info":[{"award-number":["25SF050"]}]},{"award":["25SF050"],"award-info":[{"award-number":["25SF050"]}],"id":[{"id":"https:\/\/ror.org\/01dzed356","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>With the increasing demand for sustainable animal production, the utilization of agricultural and processing by-products as functional feed ingredients has gained growing attention. However, the application of Xiasangju processing residues, a by-product generated during the industrial processing of the traditional Chinese herbal formula Xiasangju, in poultry nutrition remains poorly understood. This study aimed to evaluate the nutritional composition, bioactive components, and potential functional effects of Xiasangju processing by-products as a dietary supplement for late-laying hens. Chemical composition was characterized using LC-MS and conventional nutritional analysis, while potential anti-inflammatory mechanisms were predicted via network pharmacology. A total of 288 Jingfen laying hens (55 weeks old) were randomly assigned to four dietary treatments supplemented with 0, 0.5%, 1.0%, or 1.5% Xiasangju processing by-products for 56 days. Results showed that 11 major active compounds were identified, including relatively high levels of linarin and rosmarinic acid, along with abundant crude protein, fiber, minerals, and amino acids. Dietary supplementation at 1.5% was associated with higher egg production rate, egg weight, and yolk color without obvious adverse effects on organ indices or serum biochemical parameters. This treatment was also associated with lower inflammatory gene expression, including IL-6 and COX-2 in the ileum and NF-\u03baB, IL-6, COX-2, and TNF-\u03b1 in the cecum. No significant effects were observed on antioxidant status in yolk or liver, or intestinal morphology. Integrated analyses using network pharmacology, molecular docking, and in vitro COX-2 inhibition suggested that anti-inflammatory-related responses may involve COX-2-related pathways, while 16S rRNA sequencing indicated changes in gut microbiota composition. In conclusion, Xiasangju processing by-products may serve as a potential functional feed ingredient for late-laying hens, with 1.5% inclusion showing favorable overall performance under the conditions of this study. These effects may be associated with the combined contribution of residual nutrients and bioactive compounds in the residues.<\/jats:p>","DOI":"10.3390\/ani16121841","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T15:00:48Z","timestamp":1781535648000},"page":"1841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Xiasangju Processing Residues Improve Production Performance and Modulate Intestinal Inflammation and Gut Microbiota in Laying Hens"],"prefix":"10.3390","volume":"16","author":[{"given":"Yiwei","family":"Jin","sequence":"first","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingping","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panpan","family":"Shi","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8367-8196","authenticated-orcid":false,"given":"Peng","family":"Huang","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Chinese Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/s40104-024-01101-9","article-title":"Phytobiotics in poultry: Revolutionizing broiler chicken nutrition with plant-derived gut health enhancers","volume":"15","author":"Obianwuna","year":"2024","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hu, L., Luo, Y., Yang, J., and Cheng, C. (2025). Botanical flavonoids: Efficacy, absorption, metabolism and advanced pharmaceutical technology for improving bioavailability. Molecules, 30.","DOI":"10.3390\/molecules30051184"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nguyen, V., Taine, E.G., Meng, D., Cui, T., and Tan, W. (2024). Chlorogenic acid: A systematic review on the biological functions, mechanistic actions, and therapeutic potentials. Nutrients, 16.","DOI":"10.3390\/nu16070924"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Wang, M.-H., Liu, L., Li, J., Zhou, W.-W., Tian, W., Zhao, J.-H., and Li, X.-M. (2025). The extract from the stem and leaf of Paeonia lactiflora Pall has demonstrated an anti-oxidative stress effect in alleviating diarrhea by regulating the gut-liver axis. Antioxidants, 14.","DOI":"10.3390\/antiox14050592"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s10311-025-01837-4","article-title":"Recycling traditional Chinese medicine residues: A review","volume":"23","author":"Wu","year":"2025","journal-title":"Environ. Chem. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113607","DOI":"10.1016\/j.jenvman.2021.113607","article-title":"Treatment and bioresources utilization of traditional Chinese medicinal herb residues: Recent technological advances and industrial prospect","volume":"299","author":"Huang","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xiao, R., Wang, L., Tang, Z., Qian, X., Wang, J., Lian, Y., Tang, J., Xu, J., Lin, Y., and Shi, B. (2024). Effects of fermented Chinese herbal medicine feed additives on growth performance and intestinal microbiota of piglets. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0308196"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhou, X., Li, S., Jiang, Y., Deng, J., Yang, C., Kang, L., Zhang, H., and Chen, X. (2023). Use of fermented Chinese medicine residues as a feed additive and effects on growth performance, meat quality, and intestinal health of broilers. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1157935"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1002\/jsfa.13002","article-title":"Stepwise co-fermented traditional Chinese medicine byproducts improve antioxidant and anti-inflammatory effects in a piglet model","volume":"104","author":"Niu","year":"2024","journal-title":"J. Sci. Food Agric."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wu, S., Luo, H., Zhong, Z., Ai, Y., Zhao, Y., Liang, Q., and Wang, Y. (2022). Phytochemistry, pharmacology and quality control of Xiasangju: A traditional Chinese medicine formula. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.930813"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Pan, J., Wang, H., and Chen, Y. (2022). Prunella vulgaris L.\u2014A review of its ethnopharmacology, phytochemistry, quality control and pharmacological effects. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.903171"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1007\/s00210-023-02434-4","article-title":"Morus alba: A comprehensive phytochemical and pharmacological review","volume":"396","author":"Batiha","year":"2023","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liang, Y., Liu, T., Wang, D., and Liu, Q. (2025). Exploring the antimicrobial, anti-inflammatory, antioxidant, and immunomodulatory properties of Chrysanthemum morifolium and Chrysanthemum indicum: A narrow review. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1538311"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"105446","DOI":"10.1016\/j.psj.2025.105446","article-title":"Effects of dietary calcium source and quantity on the laying rate, eggshell quality, reproductive tract, liver fat level, and duodenum morphology in Dekalb white laying hens of 90 weeks of age","volume":"104","author":"Star","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"102618","DOI":"10.1016\/j.psj.2023.102618","article-title":"Long-term effects of dietary calcium and phosphorus level, and feed form during rearing on egg production, eggshell quality, and bone traits in brown laying hens from 30 to 89 wk of age","volume":"102","author":"Dijkslag","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"102008","DOI":"10.1016\/j.psj.2022.102008","article-title":"Intestinal microbiota of layer hens and its association with egg quality and safety","volume":"101","author":"Dai","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106241","DOI":"10.1016\/j.psj.2025.106241","article-title":"Age-related changes in intestinal mucosal barrier function in laying hens during the mid-to-late production stages","volume":"105","author":"Kuroda","year":"2026","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.aninu.2024.01.012","article-title":"Phytogenic feed additives as natural antibiotic alternatives in animal health and production: A review of the literature of the last decade","volume":"17","author":"Wang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_19","first-page":"1","article-title":"The potential of herbal feed additives in poultry nutrition","volume":"19","author":"Denli","year":"2024","journal-title":"CABI Rev."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, B., Wang, Z., Huang, C., Wang, D., Chang, D., Shi, X., Chen, Y., and Chen, H. (2022). Positive effects of Mulberry leaf extract on egg quality, lipid metabolism, serum biochemistry, and antioxidant indices of laying hens. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.1005643"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"105425","DOI":"10.1016\/j.psj.2025.105425","article-title":"Dietary compounded traditional Chinese medicine residue meal improves laying performance, egg quality, and gut barrier integrity in late-phase laying hens","volume":"104","author":"Lai","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"138233","DOI":"10.1016\/j.jclepro.2023.138233","article-title":"Herbal biochar preparation and its application as soil amendment: Is such disposal of traditional Chinese medicine residue cost-efficient towards carbon neutrality?","volume":"419","author":"Deng","year":"2023","journal-title":"J. Clean. Prod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"141","DOI":"10.14202\/vetworld.2025.141-154","article-title":"Phytogenic feed additives as alternatives to antibiotics in poultry production: A review","volume":"18","author":"Aminullah","year":"2025","journal-title":"Vet. World"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sun, W., Chen, Z., Huang, Z., Wan, A., Zhou, M., and Gao, J. (2023). Effects of dietary traditional Chinese medicine residues on growth performance, intestinal health and gut microbiota compositions in weaned piglets. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1283789"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101886","DOI":"10.1016\/j.psj.2022.101886","article-title":"Dietary resveratrol improved production performance, egg quality, and intestinal health of laying hens under oxidative stress","volume":"101","author":"Ding","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"105106","DOI":"10.1016\/j.psj.2025.105106","article-title":"Influence of purslane extract on immuno-antioxidant status, intestinal barrier, and microbiota of chicks after experimental infection with Escherichia coli O78","volume":"104","author":"Lun","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.japr.2019.12.001","article-title":"Production performance, egg quality, and small intestine histomorphology of the laying hens supplemented with phytogenic feed additive","volume":"29","author":"Sharma","year":"2020","journal-title":"J. Appl. Poult. Res."},{"key":"ref_28","first-page":"101659","article-title":"Effect of Rosmarinus officinalis supplementation on productive performance and egg quality traits in laying hens: A systematic review and meta-analysis","volume":"19","author":"Hassanpour","year":"2025","journal-title":"J. Agric. Food Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"101481","DOI":"10.1016\/j.psj.2021.101481","article-title":"Synergistic effects of quercetin and vitamin E on egg production, egg quality, and immunity in aging breeder hens","volume":"100","author":"Amevor","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Qin, B., Li, Z., Zhu, Q., Chen, T., Lan, W., Cui, Y., Azad, M.A.K., and Kong, X. (2024). Dietary fermented blueberry pomace supplementation improves small intestinal barrier function and modulates cecal microbiota in aged laying hens. Animals, 14.","DOI":"10.3390\/ani14192786"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100566","DOI":"10.1016\/j.vas.2026.100566","article-title":"Poultry gut health: Industry drivers, management determinants, and emerging research directions","volume":"31","author":"Azizi","year":"2026","journal-title":"Vet. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gu, T., Zhang, Z., Liu, J., Chen, L., Tian, Y., Xu, W., Zeng, T., Wu, W., and Lu, L. (2023). Chlorogenic acid alleviates LPS-induced inflammation and oxidative stress by modulating CD36\/AMPK\/PGC-1\u03b1 in RAW264. 7 macrophages. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713516"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"110267","DOI":"10.1016\/j.jbc.2025.110267","article-title":"Selective inhibition of TRPV3 channel by natural rosmarinic acid and its analogs for alleviation of skin lesions through downregulation of NF-\u03baB pathway","volume":"301","author":"Qu","year":"2025","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Huang, J., Xie, M., He, L., Song, X., and Cao, T. (2023). Chlorogenic acid: A review on its mechanisms of anti-inflammation, disease treatment, and related delivery systems. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1218015"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"106421","DOI":"10.1016\/j.phrs.2022.106421","article-title":"Anxiolytic and antidepressive potentials of rosmarinic acid: A review with a focus on antioxidant and anti-inflammatory effects","volume":"184","author":"Dahchour","year":"2022","journal-title":"Pharmacol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"111708","DOI":"10.1016\/j.fct.2020.111708","article-title":"Acacetin, a flavone with diverse therapeutic potential in cancer, inflammation, infections and other metabolic disorders","volume":"145","author":"Singh","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"156171","DOI":"10.1016\/j.phymed.2024.156171","article-title":"Acacetin alleviates rheumatoid arthritis by targeting HSP90 ATPase domain to promote COX-2 degradation","volume":"135","author":"Wang","year":"2024","journal-title":"Phytomedicine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5369","DOI":"10.18632\/aging.202469","article-title":"Inhibiting TLR4 signaling by linarin for preventing inflammatory response in osteoarthritis","volume":"13","author":"Qi","year":"2021","journal-title":"Aging"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"skaa090","DOI":"10.1093\/jas\/skaa090","article-title":"Effects of heat stress on the gut health of poultry","volume":"98","author":"Rostagno","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Xiang, Q., Wang, C., Zhang, H., Lai, W., Wei, H., and Peng, J. (2019). Effects of different probiotics on laying performance, egg quality, oxidative status, and gut health in laying hens. Animals, 9.","DOI":"10.3390\/ani9121110"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1038\/s41467-023-39403-7","article-title":"Gut microbiota Turicibacter strains differentially modify bile acids and host lipids","volume":"14","author":"Lynch","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Maki, J.J., Showman, L., and Looft, T. (2025). Avian and human Turicibacter isolates possess bile salt hydrolases with activity against tauro-conjugated bile acids. Bacteria, 4.","DOI":"10.3390\/bacteria4030035"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105120","DOI":"10.1016\/j.psj.2025.105120","article-title":"Effects of Bacillus subtilis ZY1 on production performance, egg quality, serum parameters and intestinal health in laying hens","volume":"104","author":"Dong","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"123856","DOI":"10.1016\/j.jclepro.2020.123856","article-title":"Comprehensive utilization of Chinese medicine residues for industry and environment protection: Turning waste into treasure","volume":"279","author":"Lu","year":"2021","journal-title":"J. Clean. Prod."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1841\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T15:27:24Z","timestamp":1781537244000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,15]]},"references-count":44,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ani16121841"],"URL":"https:\/\/doi.org\/10.3390\/ani16121841","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,15]]}}}