{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:51:09Z","timestamp":1781848269264,"version":"3.54.5"},"reference-count":46,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"crossref","award":["2025-MS-173"],"award-info":[{"award-number":["2025-MS-173"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100018617","name":"Liaoning Revitalization Talents Program","doi-asserted-by":"publisher","award":["XLYC2203164"],"award-info":[{"award-number":["XLYC2203164"]}],"id":[{"id":"10.13039\/501100018617","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Senecavirus A (SVA) is a newly emerging picornavirus associated with porcine idiopathic vesicular disease and sudden death in newborn piglets. Currently, no specific vaccines or drugs are available against SVA, highlighting the importance of investigating the immunological characteristics of its key proteins. The VP1 protein of SVA exhibits strong immunogenicity and high sequence conservation, and it is indispensable to the viral life cycle. In the present study, a monoclonal antibody (mAb) against VP1 was generated. A series of truncated VP1 proteins was then expressed to precisely map the epitope recognized by this mAb. The minimal reactive unit was identified as 16DTDFSGELA24. Homology analysis further revealed that this epitope is conserved among different SVA isolates deposited in GenBank. Moreover, AlphaFold prediction, along with PyMOL (Version 3.0.3) and GETAREA analyses, reveals that this epitope resides in the \u03b1-helix and loop regions of the three-dimensional structure of the VP1 protein and is surface-exposed. Collectively, these findings indicate that the mAb and its recognized epitope represent valuable tools for investigating SVA etiology and VP1 protein function.<\/jats:p>","DOI":"10.3390\/ani16121856","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:58:38Z","timestamp":1781661518000},"page":"1856","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a Conserved Linear Antigenic Determinant in the Senecavirus A VP1 Protein"],"prefix":"10.3390","volume":"16","author":[{"given":"Zhaogeng","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yao","sequence":"additional","affiliation":[{"name":"Liaoning Rural and Agricultural Development Service Center, 7-1 Lingyuan Street, Shenyang 110031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Zang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meitong","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baozhu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongcheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashan","family":"Sun","sequence":"additional","affiliation":[{"name":"Jiangsu Lihua Foods Group Co., Ltd., 66 Caoxi Road, Changzhou 213161, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7863-6858","authenticated-orcid":false,"given":"Xuexia","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"ref_1","first-page":"84","article-title":"Idiopathic vesicular disease in swine in Manitoba","volume":"49","author":"Pasma","year":"2008","journal-title":"Can. Vet. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1016\/j.str.2008.07.013","article-title":"Structure of Seneca Valley Virus-001: An oncolytic picornavirus representing a new genus","volume":"16","author":"Venkataraman","year":"2008","journal-title":"Structure"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"198038","DOI":"10.1016\/j.virusres.2020.198038","article-title":"Comprehensive review on immunopathogenesis, diagnostic and epidemiology of Senecavirus A","volume":"286","author":"Houston","year":"2020","journal-title":"Virus Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Leme, R.A., Alfieri, A.F., and Alfieri, A.A. (2017). Update on Senecavirus Infection in Pigs. Viruses, 9.","DOI":"10.3390\/v9070170"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"110338","DOI":"10.1016\/j.virol.2024.110338","article-title":"Oncolytic senecavirus A in tumor immunotherapy: Mechanisms, progress, and future directions","volume":"603","author":"Zhao","year":"2025","journal-title":"Virology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.virol.2023.06.004","article-title":"Structural and nonstructural proteins of Senecavirus A: Recent research advances, and lessons learned from those of other picornaviruses","volume":"585","author":"Wang","year":"2023","journal-title":"Virology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1099\/vir.0.83570-0","article-title":"Complete genome sequence analysis of Seneca Valley virus-001, a novel oncolytic picornavirus","volume":"89","author":"Hales","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1111\/tbed.12430","article-title":"Senecavirus A: An Emerging Vesicular Infection in Brazilian Pig Herds","volume":"62","author":"Leme","year":"2015","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.3201\/eid2207.151897","article-title":"Detection and Genomic Characterization of Senecavirus A, Ohio, USA, 2015","volume":"22","author":"Wang","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1111\/tbed.12557","article-title":"The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China","volume":"64","author":"Wu","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Liu, C., Liu, Y., Li, X., Liang, L., and Cui, S. (2021). Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.694110"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1111\/tbed.12669","article-title":"Emergence and whole-genome sequence of Senecavirus A in Colombia","volume":"64","author":"Sun","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/tbed.12654","article-title":"The first detection of Senecavirus A in pigs in Thailand, 2016","volume":"65","author":"Rodtian","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e3462","DOI":"10.1111\/tbed.14747","article-title":"First report and genetic characterization of Seneca Valley virus (SVV) in Chile","volume":"69","author":"Bennett","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"289","DOI":"10.54846\/jshap\/1358","article-title":"First detection and genetic characterization of Senecavirus A in pigs from Mexico","volume":"31","year":"2023","journal-title":"J. Swine Health Prod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e01247-18","DOI":"10.1128\/MRA.01247-18","article-title":"First Detection and Genome Sequence of Senecavirus A in Vietnam","volume":"8","author":"Arzt","year":"2019","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.3201\/eid2207.151863","article-title":"Vesicular Disease in 9-Week-Old Pigs Experimentally Infected with Senecavirus A","volume":"22","author":"Montiel","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2828","DOI":"10.1111\/tbed.14435","article-title":"Development of a VP2-based real-time fluorescent reverse transcription recombinase-aided amplification assay to rapidly detect Senecavirus A","volume":"69","author":"Wang","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1128\/JCM.00710-16","article-title":"Serological and Molecular Detection of Senecavirus A Associated with an Outbreak of Swine Idiopathic Vesicular Disease and Neonatal Mortality","volume":"54","author":"Rademacher","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e01927-17","DOI":"10.1128\/JVI.01927-17","article-title":"Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid","volume":"92","author":"Strauss","year":"2018","journal-title":"J. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1111\/tbed.12662","article-title":"Emergence of novel Seneca Valley virus strains in China, 2017","volume":"64","author":"Zhu","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13087","DOI":"10.1073\/pnas.1814309115","article-title":"Seneca Valley virus attachment and uncoating mediated by its receptor anthrax toxin receptor 1","volume":"115","author":"Cao","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Evans, D.J., Wasinger, A.M., Brey, R.N., Dunleavey, J.M., St Croix, B., and Bann, J.G. (2018). Seneca Valley Virus Exploits TEM8, a Collagen Receptor Implicated in Tumor Growth. Front. Oncol., 8.","DOI":"10.3389\/fonc.2018.00506"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Corbett, V., Hallenbeck, P., Rychahou, P., and Chauhan, A. (2022). Evolving role of seneca valley virus and its biomarker TEM8\/ANTXR1 in cancer therapeutics. Front. Mol. Biosci., 9.","DOI":"10.3389\/fmolb.2022.930207"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2585719","DOI":"10.1080\/21505594.2025.2585719","article-title":"Rapid detection of Seneca Valley virus based on reverse transcription recombinase polymerase amplification and CRISPR-Cas13a","volume":"16","author":"Wu","year":"2025","journal-title":"Virulence"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Wen, X.X., Sun, J.S., Wang, X.R., Bao, H.M., Zhao, Y.H., Zeng, X.Y., Xu, X.L., Ma, Y., and Chen, H.L. (2015). Identification of a novel linear epitope on the NS1 protein of avian influenza virus. BMC Microbiol., 15.","DOI":"10.1186\/s12866-015-0507-4"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1016\/j.jia.2023.04.012","article-title":"Identification of two novel linear epitopes on the p30 protein of African swine fever virus","volume":"22","author":"Yu","year":"2023","journal-title":"J. Integr. Agric."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Biol. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/(SICI)1096-987X(199802)19:3<319::AID-JCC6>3.0.CO;2-W","article-title":"Exact and efficient analytical calculation of the accessible surface areas and their gradients for macromolecules","volume":"19","author":"Fraczkiewicz","year":"1998","journal-title":"J. Comput. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Britto-Borges, T., and Barton, G.J. (2017). A study of the structural properties of sites modified by the O-linked 6-N-acetylglucosamine transferase. PLoS ONE, 12.","DOI":"10.1101\/115121"},{"key":"ref_32","first-page":"100100","article-title":"Immune evasion strategies of Seneca Valley virus: Mechanisms of host innate immune suppression","volume":"3","author":"Xie","year":"2025","journal-title":"Agric. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3763","DOI":"10.1080\/15548627.2021.1897223","article-title":"Selective autophagy receptor SQSTM1\/p62 inhibits Seneca Valley virus replication by targeting viral VP1 and VP3","volume":"17","author":"Wen","year":"2021","journal-title":"Autophagy"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1080\/15548627.2023.2277108","article-title":"Seneca Valley virus 3C protease cleaves OPTN (optineurin) to Impair selective autophagy and type I interferon signaling","volume":"20","author":"Song","year":"2024","journal-title":"Autophagy"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Gao, H., Xiang, Z.Y., Ge, X.N., Zhou, L., Han, J., Guo, X., Chen, Y.H., Zhang, Y.N., and Yang, H.C. (2022). Comparative Proteomic Analysis Reveals Mx1 Inhibits Senecavirus A Replication in PK-15 Cells by Interacting with the Capsid Proteins VP1, VP2 and VP3. Viruses, 14.","DOI":"10.3390\/v14050863"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1172\/JCI93472","article-title":"Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus","volume":"127","author":"Miles","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e01550-21","DOI":"10.1128\/JVI.01550-21","article-title":"Synergetic Contributions of Viral VP1, VP3, and 3C to Activation of the AKT-AMPK-MAPK-MTOR Signaling Pathway for Seneca Valley Virus-Induced Autophagy","volume":"96","author":"Song","year":"2022","journal-title":"J. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e01717","DOI":"10.1128\/JVI.01717-17","article-title":"Adaptive Immune Responses following Senecavirus A Infection in Pigs","volume":"92","author":"Maggioli","year":"2018","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"108753","DOI":"10.1016\/j.vetmic.2020.108753","article-title":"Identification of linear B cell epitopes on VP1 and VP2 proteins of (SVA) using monoclonal antibodies","volume":"247","author":"Fan","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.virol.2023.03.015","article-title":"Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine","volume":"582","author":"Zhang","year":"2023","journal-title":"Virology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"D436","DOI":"10.1093\/nar\/gkae1092","article-title":"The Immune Epitope Database (IEDB): 2024 update","volume":"53","author":"Vita","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"110266","DOI":"10.1016\/j.vetmic.2024.110266","article-title":"Characterization of the immunodominant regions of Senecavirus A-VP1 structural protein via ELISA epitope mapping","volume":"298","author":"Houston","year":"2024","journal-title":"Vet. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"110789","DOI":"10.1016\/j.vetmic.2025.110789","article-title":"Identification of transiently produced IgG linear epitopes in Senecavirus A for differentiating infected from vaccinated animals","volume":"312","author":"Yang","year":"2026","journal-title":"Vet. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110198","DOI":"10.1016\/j.vetmic.2024.110198","article-title":"A nanoparticle vaccine based on the VP1(21-26) and VP2 structural proteins of Senecavirus A induces robust protective immune responses","volume":"296","author":"Cao","year":"2024","journal-title":"Vet. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2165\/00063030-200721030-00002","article-title":"Epitope mapping: The first step in developing epitope-based vaccines","volume":"21","author":"Gershoni","year":"2007","journal-title":"BioDrugs"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110636","DOI":"10.1016\/j.vetmic.2025.110636","article-title":"Identification of conformational epitopes on VP1 of Senecavirus A by monoclonal antibodies and phage display","volume":"308","author":"Tian","year":"2025","journal-title":"Vet. Microbiol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1856\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:00:48Z","timestamp":1781845248000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":46,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ani16121856"],"URL":"https:\/\/doi.org\/10.3390\/ani16121856","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,16]]}}}