{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:51:20Z","timestamp":1781931080961,"version":"3.54.5"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","award":["D18007"],"award-info":[{"award-number":["D18007"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Mycoplasma synoviae (MS) poses a continuous threat to the poultry industry in Mainland China, yet a comprehensive nationwide assessment remains lacking. This systematic review and meta-analysis quantified the pooled prevalence of MS and evaluated key epidemiological characteristics and sources of heterogeneity. Peer-reviewed studies were retrieved from multiple databases, and random-effects models were utilized to estimate and compare pooled seroprevalence and molecular detection prevalence. The results revealed a heavy MS infection burden characterized by a distinct diagnostic disparity: a high serological prevalence co-existed with a moderate molecular detection prevalence, reflecting widespread past exposure, chronic carrying, or vaccination. Geographically, the Northwest region exhibited the highest seroprevalence (61.8%), though inter-regional differences were not statistically significant (p = 0.152). Among production types, breeder flocks maintained a relatively high pooled prevalence of 69.6% (95% CI: 40.5\u201392.0%), although differences among production types were not statistically significant. Subgroup analysis demonstrated a statistically significant difference among age groups; however, the available data did not support definitive conclusions regarding age-specific risk patterns. Breeder flocks exhibited a relatively higher pooled prevalence than layers and broilers, suggesting a potential epidemiological role that warrants further investigation. In conclusion, MS appears to be widely distributed in chickens in mainland China. Differences between the serological and molecular detection results highlight the complexity of interpreting MS prevalence estimates. Given the substantial heterogeneity among studies, the findings should be interpreted cautiously. Future studies incorporating standardized surveillance and detailed vaccination histories are needed to better understand MS epidemiology and support evidence-based control strategies.<\/jats:p>","DOI":"10.3390\/ani16121893","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T10:25:34Z","timestamp":1781778334000},"page":"1893","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Epidemiological Characteristics of Mycoplasma synoviae Infection in Chickens in Mainland China"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8709-581X","authenticated-orcid":false,"given":"Xinyuan","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9327-1804","authenticated-orcid":false,"given":"Huiling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Independent Researcher, New York, NY 11355, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5170-6298","authenticated-orcid":false,"given":"Tangjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sun, S.K., Lin, X., Chen, F., Wang, D.A., Lu, J.P., Qin, J.P., and Luo, T.R. (2017). Epidemiological investigation of Mycoplasma synoviae in native chicken breeds in China. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1029-0"},{"key":"ref_2","first-page":"1","article-title":"Field observations on Mycoplasma synoviae infections and eggshell apex abnormalities","volume":"44","author":"Landman","year":"2015","journal-title":"Avian Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/03079457.2021.1944605","article-title":"Clinical expression, epidemiology, and monitoring of Mycoplasma gallisepticum and Mycoplasma synoviae: An update","volume":"51","author":"Feberwee","year":"2022","journal-title":"Avian Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1080\/03079457.2024.2419037","article-title":"Mycoplasma gallisepticum and Mycoplasma synoviae in commercial poultry: Current control strategies and future challenges","volume":"54","author":"Feberwee","year":"2025","journal-title":"Avian Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Galluzzo, P., Migliore, S., Galuppo, L., Condorelli, L., Hussein, H.A., Licitra, F., and Loria, G.R. (2022). First molecular survey to detect Mycoplasma gallisepticum and Mycoplasma synoviae in poultry farms in a strategic production district of Sicily (South Italy). Animals, 12.","DOI":"10.3390\/ani12080962"},{"key":"ref_6","first-page":"100982","article-title":"Seroprevalence and prevalence of Mycoplasma synoviae in laying hens and broiler breeders in Spain","volume":"100","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"51","DOI":"10.24099\/vet.arhiv.1490","article-title":"Monitoring reveals a high prevalence of Mycoplasma synoviae in layer flocks in Croatia","volume":"93","author":"Gottstein","year":"2023","journal-title":"Vet. Arh."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.3382\/ps\/pex134","article-title":"Serological investigation of Mycoplasma synoviae infection in China from 2010 to 2015","volume":"96","author":"Xue","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105860","DOI":"10.1016\/j.psj.2025.105860","article-title":"Research note: Prevalence and vlhA gene genotyping analysis of Mycoplasma synoviae in poultry from 2021 to 2023 in central China","volume":"104","author":"Zhang","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Tu, L., Li, X., Liu, Y., Jin, X., Yin, L., Wang, X., Zhou, Q., Wang, K., and Tang, Y. (2026). Genetic diversity and emerging trends of Mycoplasma synoviae in China: Insights from a 2024 nationwide MLST study. Microorganisms, 14.","DOI":"10.3390\/microorganisms14010257"},{"key":"ref_11","first-page":"9","article-title":"Epidemiological investigation of Mycoplasma synoviae in chickens and drug sensitivity analysis of isolates from different regions","volume":"55","author":"Zhao","year":"2019","journal-title":"Chin. J. Vet. Med."},{"key":"ref_12","first-page":"81","article-title":"Genetic evolution analysis of the vlhA gene of Mycoplasma synoviae isolates from some regions of China during 2016\u20132019","volume":"41","author":"Xie","year":"2021","journal-title":"Chin. J. Vet. Sci."},{"key":"ref_13","first-page":"2704","article-title":"Comparison of pathogenicity of different infection routes of Mycoplasma synoviae","volume":"48","author":"Hou","year":"2021","journal-title":"Microbiol. China"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Aromataris, E., and Munn, Z. (2020). Chapter 7: Systematic reviews of etiology and risk. JBI Manual for Evidence Synthesis, JBI.","DOI":"10.46658\/JBIRM-17-01"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e178","DOI":"10.1002\/hsr2.178","article-title":"Arcsine-based transformations for meta-analysis of proportions: Pros, cons, and alternatives","volume":"3","author":"Lin","year":"2020","journal-title":"Health Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105730","DOI":"10.1016\/j.prevetmed.2022.105730","article-title":"Duck hepatitis A virus prevalence in mainland China between 2009 and 2021: A systematic review and meta-analysis","volume":"208","author":"Zhou","year":"2022","journal-title":"Prev. Vet. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.2307\/2533446","article-title":"Operating characteristics of a rank correlation test for publication bias","volume":"50","author":"Begg","year":"1994","journal-title":"Biometrics"},{"key":"ref_18","first-page":"46","article-title":"Epidemiological investigation of mycoplasma infection in broiler breeder chickens and preventive effect of Shuanghuanglian","volume":"35","author":"Pan","year":"2014","journal-title":"Feed Ind."},{"key":"ref_19","unstructured":"Wang, L. (2015). Epidemiological Investigation of Salmonella pullorum and Mycoplasma synoviae in Some Breeder Farms in Shandong Province. [Master\u2019s Thesis, Shandong Agricultural University]."},{"key":"ref_20","first-page":"73","article-title":"Serological investigation of Mycoplasma synoviae infection in chickens in some areas of Jiangsu Province","volume":"37","author":"Ding","year":"2015","journal-title":"China Poult."},{"key":"ref_21","first-page":"234","article-title":"Epidemiological investigation and analysis of Mycoplasma synoviae infection in chickens in Ningxia region","volume":"42","author":"Chen","year":"2020","journal-title":"China J. Prev. Vet. Med."},{"key":"ref_22","first-page":"10","article-title":"Pathogen detection and serological investigation of Mycoplasma synoviae in Yunnan Province","volume":"6","author":"Wang","year":"2020","journal-title":"Yunnan Anim. Husb. Vet. Med."},{"key":"ref_23","first-page":"25","article-title":"Serological investigation of Mycoplasma gallisepticum and Mycoplasma synoviae in Beijing","volume":"37","author":"Jiang","year":"2020","journal-title":"China Anim. Health Insp."},{"key":"ref_24","first-page":"52","article-title":"Epidemiological investigation of Mycoplasma synoviae in Hexi region of Gansu Province","volume":"40","author":"Nie","year":"2021","journal-title":"J. Anim. Sci. Vet. Med."},{"key":"ref_25","unstructured":"Cheng, Q.Q. (2020). Serological Detection and Isolation Identification of Avian Mycoplasma in Parts of Shanxi Province. [Master\u2019s Thesis, Shanxi Agricultural University]."},{"key":"ref_26","first-page":"86","article-title":"Investigation report on the prevalence of Mycoplasma synoviae in free-range chickens in Pukou District","volume":"32","author":"Nie","year":"2021","journal-title":"Livest. Poult. Ind."},{"key":"ref_27","unstructured":"Zhang, L.D. (2022). Epidemiological Investigation and vlhA Gene Evolutionary Analysis of Mycoplasma synoviae in Liaozhong Area. [Master\u2019s Thesis, Shenyang Agricultural University]."},{"key":"ref_28","first-page":"82","article-title":"Serological investigation and analysis of Mycoplasma synoviae infection in chickens in parts of Ningxia from 2020 to 2021","volume":"41","author":"Sun","year":"2023","journal-title":"Heilongjiang Anim. Sci. Vet. Med."},{"key":"ref_29","first-page":"76","article-title":"Investigation on infection and vertical transmission of Mycoplasma gallisepticum and Mycoplasma synoviae in chicken farms in parts of Anhui Province","volume":"46","author":"Shen","year":"2024","journal-title":"China Poult."},{"key":"ref_30","unstructured":"Wang, S. (2025). Analysis and Control of Mycoplasma synoviae Infection in a Broiler Breeder Farm in Liaoning. [Master\u2019s Thesis, Shenyang Agricultural University]."},{"key":"ref_31","first-page":"169","article-title":"Serological investigation and analysis of Mycoplasma synoviae infection in Yiyang region","volume":"19","author":"Sun","year":"2023","journal-title":"China Poult. Breed."},{"key":"ref_32","first-page":"18","article-title":"Serological investigation and analysis of Mycoplasma synoviae in laying hens in Bijiang District, Tongren City","volume":"3","author":"Wang","year":"2024","journal-title":"Yunnan Anim. Husb. Vet. Med."},{"key":"ref_33","first-page":"33","article-title":"Investigation, analysis, and prevention recommendations for Mycoplasma synoviae infection in non-immunized laying hens in Tianjin","volume":"2","author":"Wang","year":"2024","journal-title":"Shanghai J. Anim. Husb. Vet. Med."},{"key":"ref_34","first-page":"89","article-title":"Investigation and analysis of Salmonella pullorum, Mycoplasma synoviae, and Mycoplasma gallisepticum infections in Lueyang black-bone chicken breeder flocks in Shaanxi Province","volume":"70","author":"Shi","year":"2024","journal-title":"Shaanxi J. Agric. Sci."},{"key":"ref_35","unstructured":"Hu, R.S. (2020). Investigation and Control of Mycoplasma synoviae Infection in a Broiler Breeder Farm. [Master\u2019s Thesis, Hunan Agricultural University]."},{"key":"ref_36","first-page":"59","article-title":"Establishment and application of a TaqMan-MGB duplex real-time PCR assay for detection of Mycoplasma gallisepticum and Mycoplasma synoviae","volume":"44","author":"Han","year":"2022","journal-title":"China Poult."},{"key":"ref_37","unstructured":"Sui, C.G. (2022). Molecular Epidemiological Investigation and Drug Resistance Gene Screening Analysis of Mycoplasma synoviae. [Master\u2019s Thesis, Nanyang Normal University]."},{"key":"ref_38","unstructured":"Li, Y. (2022). Prevalence and Infection Correlation of Cryptosporidium and Mycoplasma synoviae in Intensive Chicken Farms in Guangdong Province. [Master\u2019s Thesis, Foshan University]."},{"key":"ref_39","unstructured":"Liu, T.J. (2023). Etiological Investigation and Genome Sequence Analysis of Mycoplasma synoviae in Central China. [Master\u2019s Thesis, Hebei University of Engineering]."},{"key":"ref_40","first-page":"1117","article-title":"Epidemiological investigation of Mycoplasma gallisepticum and Mycoplasma synoviae in commercial laying hens in Xinjiang, China, from 2022 to 2023","volume":"46","author":"Ma","year":"2024","journal-title":"China J. Prev. Vet. Med."},{"key":"ref_41","first-page":"28","article-title":"Epidemiological investigation of avian mycoplasmosis in Xihe County","volume":"45","author":"Lu","year":"2024","journal-title":"Shandong J. Anim. Sci. Vet. Med."},{"key":"ref_42","first-page":"107","article-title":"Monitoring and analysis of mycoplasma eradication in large-scale chicken farms in Rizhao, Shandong Province, from 2017 to 2021","volume":"21","author":"Liu","year":"2022","journal-title":"Anim. Husb. Feed Sci."},{"key":"ref_43","first-page":"73","article-title":"Serological and molecular detection of Mycoplasma synoviae infection in yellow-feathered broiler farms in Guangxi Province","volume":"41","author":"Yang","year":"2019","journal-title":"China Poult."},{"key":"ref_44","first-page":"697","article-title":"Epidemiological investigation of Mycoplasma synoviae infection in non-immunized chicken flocks in Fujian Province from 2018 to 2020","volume":"44","author":"Wang","year":"2022","journal-title":"China J. Prev. Vet. Med."},{"key":"ref_45","first-page":"38","article-title":"Epidemiological investigation of Mycoplasma synoviae in eight laying hen farms in Yancheng region","volume":"5","author":"Chen","year":"2021","journal-title":"Shanghai J. Anim. Husb. Vet. Med."},{"key":"ref_46","unstructured":"Xue, C.L. (2024). Epidemiological Investigation of MG and MS Infection in Wenchang Chicken Farms in Hainan Province and Establishment of a Duplex Fluorescent Quantitative PCR Detection Method. [Master\u2019s Thesis, Xinjiang Agricultural University]."},{"key":"ref_47","first-page":"103","article-title":"Epidemiological investigation of Mycoplasma gallisepticum and Mycoplasma synoviae in breeder farms in parts of Shandong Province","volume":"47","author":"Wang","year":"2025","journal-title":"China Poult."},{"key":"ref_48","unstructured":"Wang, C. (2025). Isolation and Identification, Epidemiological Investigation, and Pathobiological and Omics Characteristics of Experimental Infection with Mycoplasma synoviae in SPF Laying Hens. [Master\u2019s Thesis, Huazhong Agricultural University]."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.0006-341X.2000.00455.x","article-title":"Trim and fill: A simple funnel-plot-based method of testing and adjusting for publication bias in meta-analysis","volume":"56","author":"Duval","year":"2000","journal-title":"Biometrics"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"35","DOI":"10.3382\/ps\/pew228","article-title":"Rapid and sensitive detection of Mycoplasma synoviae by an insulated isothermal PCR-based assay on a field-deployable device","volume":"96","author":"Kuo","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"304349","DOI":"10.4061\/2011\/304349","article-title":"Comparative study of serological tests for Mycoplasma synoviae diagnosis in commercial poultry breeders","volume":"2011","author":"Luciano","year":"2011","journal-title":"Vet. Med. Int."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/03079457.2017.1296105","article-title":"Development, validation and field evaluation of a quantitative real-time PCR able to differentiate between field Mycoplasma synoviae and the MS-H-live vaccine strain","volume":"46","author":"Dijkman","year":"2017","journal-title":"Avian Pathol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Swayne, D.E. (2020). Mycoplasma synoviae. Diseases of Poultry, Wiley Blackwell. [14th ed.].","DOI":"10.1002\/9781119371199"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"103690","DOI":"10.1016\/j.psj.2024.103690","article-title":"Antimicrobial resistance (AMR): An important one health issue for layer and meat poultry industries worldwide","volume":"103","author":"Morrow","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"107166","DOI":"10.1016\/j.psj.2026.107166","article-title":"European Ring Test on the Differentiation of Wild-Type Mycoplasma synoviae and the MS-H Vaccine Strains in Clinical Samples","volume":"105","author":"Kreizinger","year":"2026","journal-title":"Poult. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1080\/03079457.2024.2358904","article-title":"Quantification of the Effect of Vaccination on the Control of Horizontal Transmission of Mycoplasma synoviae under Field Conditions","volume":"53","author":"Feberwee","year":"2024","journal-title":"Avian Pathol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1893\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:22:40Z","timestamp":1781929360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/12\/1893"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":56,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ani16121893"],"URL":"https:\/\/doi.org\/10.3390\/ani16121893","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,18]]}}}