{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T05:28:17Z","timestamp":1782970097239,"version":"3.54.5"},"reference-count":43,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFD1300304"],"award-info":[{"award-number":["2021YFD1300304"]}]},{"name":"Wuhan Sunhy Biology Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated the responses of growing pigs to a matched compound enzyme in two feeding systems: a corn\u2013soybean meal diet and a diversified diet. Two independent experiments were conducted, each using 80 pigs (Duroc \u00d7 [Landrace \u00d7 Yorkshire]; average body weight, 33.0 kg), which were assigned to two treatments in a randomized complete block design (RCBD) according to initial body weight and sex, with five replicate pens per treatment and eight pigs per pen. In Exp. 1, pigs were fed a corn\u2013soybean meal diet without or with 0.02% compound enzyme formulated (including xylanase, \u03b2-glucanase, \u03b2-mannanase, cellulase and pectinase) for this diet type. In Exp. 2, pigs were fed a diversified diet without or with 0.02% compound enzyme (including xylanase, \u03b2-glucanase, \u03b2-mannanase, cellulase, pectinase, amylase and protease). The supplemental level of the compound enzyme was selected according to the substrate characteristics of each diet, especially the content of fibrous components, and was further aligned with the inclusion rate recommended for practical feed production. Growth performance, apparent total tract digestibility (ATTD), serum immune and inflammatory indices, jejunal digestive enzyme activities, and cecal microbiota were evaluated. In Exp. 2, the G:F was higher during days 15\u201328 (p &lt; 0.05) and tended to increase the overall G:F (p = 0.06). In Exp. 1, dietary compound enzyme supplementation increased the ATTD of dry matter (DM), ether extract (EE), and gross energy (GE) on day 28 (p &lt; 0.05), elevated serum immunoglobulin A (IgA) concentration (p &lt; 0.05) on day 14, reduced serum concentrations of interleukin (IL)-1\u03b2, IL-6, and IL-8 on day 28 (p &lt; 0.05), and increased jejunal amylase activity (p &lt; 0.05). In Exp. 2, dietary compound enzyme supplementation increased the ATTD of DM, organic matter (OM), crude protein (CP), and GE (p &lt; 0.05), and enhanced the activities of amylase and chymotrypsin on day 28 (p &lt; 0.05). Enzyme supplementation reduced the abundance of Erysipelotrichaceae in Exp. 1 (p &lt; 0.05) but increased the abundance of Chlamydiaceae in Exp. 2 (p &lt; 0.05). In conclusion, matched compound enzyme supplementation improved nutrient utilization in both diet systems, but the response profiles differed. The diversified diet system showed clearer improvements in feed efficiency and protein-related digestibility.<\/jats:p>","DOI":"10.3390\/ani16131978","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T02:16:44Z","timestamp":1782699404000},"page":"1978","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Matched Compound Enzyme on Nutrient Utilization and Physiological Responses in Growing Pigs Fed a Corn\u2013Soybean Meal or Diversified Diet"],"prefix":"10.3390","volume":"16","author":[{"given":"Shuang","family":"Dong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijun","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuhan Sunhy Biology Co., Ltd., Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingfang","family":"Gu","sequence":"additional","affiliation":[{"name":"Wuhan Sunhy Biology Co., Ltd., Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaofen","family":"Yao","sequence":"additional","affiliation":[{"name":"Beijing Wellhope Animal Husbandry Co., Ltd., Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxi","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s11250-021-02762-8","article-title":"Cottonseed meal with enzyme complex for finishing pigs","volume":"53","author":"Corassa","year":"2021","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1439-0396.2006.00664.x","article-title":"Responses of non-starch polysaccharide-degrading enzymes on digestibility and performance of growing pigs fed a diet based on corn, soya bean meal and Chinese double-low rapeseed meal","volume":"91","author":"Fang","year":"2007","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.2527\/jas.2010-3430","article-title":"Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs","volume":"90","author":"Jo","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/ps\/84.1.37","article-title":"Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance","volume":"84","author":"Meng","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.5713\/ajas.2003.1339","article-title":"Effects of phytase and carbohydrases supplementation to diet with a partial replacement of soybean meal with rapeseed meal and cottonseed meal on growth performance and nutrient digestibility of growing pigs","volume":"16","author":"Shim","year":"2003","journal-title":"Asian-Aust. J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.livsci.2010.06.103","article-title":"Effects of viscosity and fermentability of purified non-starch polysaccharides on ileal and total tract nutrient digestibility in ileal-cannulated grower pigs","volume":"134","author":"Hooda","year":"2010","journal-title":"Livest. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.anifeedsci.2017.11.009","article-title":"The combination of carbohydrases and phytase to improve nutritional value and non-starch polysaccharides degradation for growing pigs fed diets with or without wheat bran","volume":"235","author":"Zeng","year":"2018","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"628","DOI":"10.3382\/japr.2013-00739","article-title":"Feed enzymes: The science, practice, and metabolic realities","volume":"22","author":"Ravindran","year":"2013","journal-title":"J. Appl. Poult. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1002\/jsfa.10806","article-title":"Improving the digestibility of cereal fractions of wheat, maize, and rice by a carbohydrase complex rich in xylanases and arabinofuranosidases: An in vitro digestion study","volume":"101","author":"Vangsoe","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.3382\/ps\/pev244","article-title":"Starch digestibility, energy utilization, and growth performance of broilers fed corn-soybean basal diets supplemented with enzymes","volume":"94","author":"Stefanello","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101052","DOI":"10.1016\/j.animal.2023.101052","article-title":"Dietary supplementation of proteases on growth performance, nutrient digestibility, blood characteristics and gut microbiota of growing pigs fed sorghum-based diets","volume":"18","author":"Peng","year":"2024","journal-title":"Animal"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"739134","DOI":"10.1016\/j.aquaculture.2022.739134","article-title":"Effect of xylanase and \u03b2-glucanase on growth performance, activity of digestive enzymes, digestibility, and microbiome diversity of juvenile Nile tilapia fed soybean meal and\/or sorghum distillers dried grains with solubles-based diets","volume":"565","author":"Urbich","year":"2023","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Recharla, N., Kim, D., Ramani, S., Song, M., Park, J., Balasubramanian, B., Puligundla, P., and Park, S. (2019). Dietary multi-enzyme complex improves In Vitro nutrient digestibility and hind gut microbial fermentation of pigs. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0217459"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chen, Y., Shen, D., Zhang, L., Zhong, R., Liu, Z., Liu, L., Chen, L., and Zhang, H. (2020). Supplementation of Non-Starch Polysaccharide Enzymes Cocktail in a Corn-Miscellaneous Meal Diet Improves Nutrient Digestibility and Reduces Carbon Dioxide Emissions in Finishing Pigs. Animals, 10.","DOI":"10.3390\/ani10020232"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"skae064","DOI":"10.1093\/jas\/skae064","article-title":"Efficacy of a novel multi-enzyme feed additive on growth performance, nutrient digestibility, and gut microbiome of weanling pigs fed corn-wheat or wheat-barley-based diet","volume":"102","author":"Aderibigbe","year":"2024","journal-title":"J. Anim. Sci."},{"key":"ref_16","unstructured":"NRC (2012). Nutrient Requirements of Swine, National Academy Press. [11th ed.]."},{"key":"ref_17","unstructured":"AOAC (2007). Official Methods of Analysis, AOAC International. [18th ed.]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"skab369","DOI":"10.1093\/jas\/skab369","article-title":"Nutritional evaluation of fish protein hydrolysate and its application in piglet production","volume":"100","author":"Zhang","year":"2022","journal-title":"J. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Valente Junior, D.T., Genova, J.L., Kim, S.W., Saraiva, A., and Rocha, G.C. (2024). Carbohydrases and Phytase in Poultry and Pig Nutrition: A Review beyond the Nutrients and Energy Matrix. Animals, 14.","DOI":"10.3390\/ani14020226"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10150","DOI":"10.1038\/s41598-019-46341-2","article-title":"Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)","volume":"9","author":"Staack","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/1745039X.2020.1852008","article-title":"Effects of dietary supplementation of compound enzyme on performance, nutrient digestibility, serum antioxidant status, immunoglobulins, intestinal morphology and microbiota community in weaned pigs","volume":"75","author":"Long","year":"2021","journal-title":"Arch. Anim. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"528","DOI":"10.3382\/ps\/pev333","article-title":"Impact of combined beta-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets","volume":"95","author":"Munyaka","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.anifeedsci.2013.05.006","article-title":"Protease supplementation of sorghum-based broiler diets enhances amino acid digestibility coefficients in four small intestinal sites and accelerates their rates of digestion","volume":"183","author":"Liu","year":"2013","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1111\/jpn.13523","article-title":"Low-tannin sorghum grain could be used as an alternative to corn in diet for nursery pigs","volume":"105","author":"Pan","year":"2021","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"998","DOI":"10.5713\/ajas.15.0886","article-title":"Performance Responses, Nutrient Digestibility, Blood Characteristics, and Measures of Gastrointestinal Health in Weanling Pigs Fed Protease Enzyme","volume":"29","author":"Tactacan","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5054","DOI":"10.2527\/jas2017.1662","article-title":"Viscous and fermentable nonstarch polysaccharides affect intestinal nutrient and energy flow and hindgut fermentation in growing pigs","volume":"95","author":"Chen","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.anifeedsci.2005.09.002","article-title":"The effect of dietary crude protein level, cereal type and exogenous enzyme supplementation on nutrient digestibility, nitrogen excretion, faecal volatile fatty acid concentration and ammonia emissions from pigs","volume":"127","author":"Callan","year":"2006","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_28","first-page":"4018","article-title":"Effects of a multi-strain Bacillus spp. direct-fed microbial and a protease enzyme on growth performance, nutrient digestibility, blood characteristics, fecal microbiota, and noxious gas emissions of grower pigs fed corn-soybean-meal-based diets-A meta-analysis","volume":"95","author":"Payling","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.2527\/jas.2015-9027","article-title":"Effect of supplementing a fibrous diet with a xylanase and beta-glucanase blend on growth performance, intestinal glucose uptake, and transport-associated gene expression in growing pigs","volume":"93","author":"Agyekum","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_30","first-page":"135","article-title":"Enzyme supplementation of wheat, barley or sugar beet pulp based diets for early weaned piglets: Effects on performance and faecal nutrient digestibility","volume":"1992","author":"Mellange","year":"2017","journal-title":"Proc. Br. Soc. Anim. Prod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.5713\/ajas.2013.13129","article-title":"The effects of enzyme complex on performance, intestinal health and nutrient digestibility of weaned pigs","volume":"26","author":"Yi","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Liu, Y., Cheng, X., Zhen, W., Zeng, D., Qu, L., Wang, Z., and Ning, Z. (2021). Yeast Culture Improves Egg Quality and Reproductive Performance of Aged Breeder Layers by Regulating Gut Microbes. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.633276"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"103940","DOI":"10.1016\/j.livsci.2020.103940","article-title":"Effects of xylanase on growth performance, nutrients digestibility and intestinal health in weaned piglets","volume":"233","author":"He","year":"2020","journal-title":"Livest. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1079\/BJN20041206","article-title":"Addition of pearl barley to a rice-based diet for newly weaned piglets increases the viscosity of the intestinal contents, reduces starch digestibility and exacerbates post-weaning colibacillosis","volume":"92","author":"Hopwood","year":"2004","journal-title":"Br. J. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.anifeedsci.2018.07.002","article-title":"Supplemental effect of xylanase and mannanase on nutrient digestibility and gut health of nursery pigs studied using both in vivo and in vitro models","volume":"245","author":"Tiwari","year":"2018","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1439-0396.2008.00893.x","article-title":"Effects of extrusion and supplementation of exogenous enzymes to diets containing Chinese storage brown rice on the carbohydrase activity in the digestive tract of piglets","volume":"94","author":"He","year":"2010","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"270","DOI":"10.5713\/ajas.2008.70289","article-title":"Effects of enzyme supplementation on growth, intestinal content viscosity, and digestive enzyme activities in growing pigs fed rough rice-based diet","volume":"21","author":"Wang","year":"2008","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/nature11550","article-title":"Diversity, stability and resilience of the human gut microbiota","volume":"489","author":"Lozupone","year":"2012","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chang, Z., Bo, S., Xiao, Q., Wang, Y., Wu, X., He, Y., Iqbal, M., Ye, Y., and Shang, P. (2022). Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1055146"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3089","DOI":"10.1093\/jas\/skz165","article-title":"Effects of dietary exogenous fibrolytic enzymes on ruminal fermentation characteristics of beef steers fed high- and low-quality growing diets1","volume":"97","author":"Kondratovich","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1297-9716-42-29","article-title":"Chlamydiaceae infections in pig","volume":"42","author":"Schautteet","year":"2011","journal-title":"Vet. Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Pandey, S., Kim, E.S., Cho, J.H., Song, M., Doo, H., Kim, S., Keum, G.B., Kwak, J., Ryu, S., and Choi, Y. (2023). Swine gut microbiome associated with non-digestible carbohydrate utilization. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1231072"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"776","DOI":"10.4014\/jmb.2203.03026","article-title":"Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets","volume":"32","author":"Lee","year":"2022","journal-title":"J. Microbiol. Biotechnol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/13\/1978\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:13:38Z","timestamp":1782965618000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/13\/1978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":43,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16131978"],"URL":"https:\/\/doi.org\/10.3390\/ani16131978","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}