{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:42:09Z","timestamp":1785404529700,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Guayaquil","award":["R-CSU-UG-SE21-111-20-05-2022"],"award-info":[{"award-number":["R-CSU-UG-SE21-111-20-05-2022"]}]},{"award":["R-CSU-UG-SE21-111-20-05-2022"],"award-info":[{"award-number":["R-CSU-UG-SE21-111-20-05-2022"]}],"id":[{"id":"https:\/\/ror.org\/047kyg834","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Spirometra mansoni causes sparganosis in humans and spirometrosis in domestic dogs. It is transmitted through the consumption of raw or undercooked meat from fish, frogs or paratenic animals, producing subcutaneous and tissue infections in humans, whereas dogs or cats develop gastrointestinal infections. Sparganosis is a zoonotic parasitosis associated with freshwater aquatic environments, prevalent in tropical and subtropical regions of the world. The purpose of this investigation was to identify S. mansoni in domestic dogs from riverine sectors of the Daule River in Ecuador, using coproparasitological methods: direct examination, flotation and sedimentation with centrifugation using saline solution (as screening); and for confirmation, morphometric methods and PCR were used. Through a descriptive, prospective and cross-sectional study, 402 domestic dogs were analyzed, and Spirometra mansoni were determined in 67 (16.66%) of the collected samples. The clinical and epidemiological characteristics of spirometrosis in dogs and the potential risk of sparganosis occurring in humans were determined. However, cases may appear in pets from other locations.<\/jats:p>","DOI":"10.3390\/ani16132049","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T07:59:47Z","timestamp":1783065587000},"page":"2049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence of Spirometra mansoni in Domestic Dogs from Rural Ecuador and Its Public Health Relevance"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5152-2843","authenticated-orcid":false,"given":"Roberto D.","family":"Coello Peralta","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine and Zootechnics, Universidad de Guayaquil, Guayaquil 090514, Ecuador"},{"name":"Instituto de Gen\u00e9tica y Microbiolog\u00eda, Fundaci\u00f3n Miguel Lillo, San Miguel de Tucum\u00e1n T4000JFE, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9288-0750","authenticated-orcid":false,"given":"Zully","family":"Baquerizo Orrala","sequence":"additional","affiliation":[{"name":"Consultorio Zu Veterinaria, Comuna San Pablo, Calle 9 de Octubre y Abd\u00f3n Calder\u00f3n, Santa Elena 241702, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-1406","authenticated-orcid":false,"given":"Aldo Rub\u00e9n","family":"Andrada","sequence":"additional","affiliation":[{"name":"Instituto de Gen\u00e9tica y Microbiolog\u00eda, Fundaci\u00f3n Miguel Lillo, San Miguel de Tucum\u00e1n T4000JFE, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6889-2765","authenticated-orcid":false,"given":"Davis","family":"Calle Atariguana","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine and Zootechnics, Universidad de Guayaquil, Guayaquil 090514, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6953-4743","authenticated-orcid":false,"given":"Geraldine","family":"Ramallo","sequence":"additional","affiliation":[{"name":"Instituto de Invertebrados, Fundaci\u00f3n Miguel Lillo, San Miguel de Tucum\u00e1n T4000JFE, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2007-7198","authenticated-orcid":false,"given":"Alicia","family":"Rojas","sequence":"additional","affiliation":[{"name":"Laboratory of Helminthology, Centro de Investigaci\u00f3n en Enfermedades Tropicales, Universidad de Costa Rica, San Jos\u00e9 2060, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3347\/kjp.2018.56.4.359","article-title":"Molecular Genetic Findings of Spirometra decipiens and S. ranarum in Korea","volume":"56","author":"Sun","year":"2018","journal-title":"Korean J. Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"457","DOI":"10.4067\/S0716-10182015000500014","article-title":"The sparganosis","volume":"32","year":"2015","journal-title":"Rev. Chil. Infectol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/vms3.932","article-title":"The global prevalence of Spirometra parasites in snakes, frogs, dogs, and cats: A systematic review and meta-analysis","volume":"8","author":"Badri","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ijid.2016.10.013","article-title":"Prevalence of Spirometra mansoni in dogs, cats, and frogs and its medical relevance in Guangzhou, China","volume":"53","author":"Hong","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_5","unstructured":"Sykes, J.E. (2024). \u00a0Spirometra masonoides. Greene\u2019s Infectious Diseases of the Dog and Cat, Saunders. [5th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100947","DOI":"10.1016\/j.ijppaw.2024.100947","article-title":"Diversity and biology of Spirometra tapeworms (Cestoda: Diphyllobothriidea), zoonotic parasites of wildlife: A review","volume":"24","author":"Kuchta","year":"2024","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_7","first-page":"101030","article-title":"Molecular, morphological and histopathological evidence of Spirometra mansoni in wild and domestic animals from Costa Rica","volume":"51","author":"Alvarado","year":"2024","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"774","DOI":"10.2307\/3279487","article-title":"On the occurrence of Spirometra mansonoides in South America","volume":"61","author":"Mueller","year":"1975","journal-title":"J. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e012123","DOI":"10.1590\/s1984-29612023065","article-title":"Endoparasites in domestic cats (Felis catus) in the semi-arid region of Northeast Brazil","volume":"32","author":"Silva","year":"2023","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14411\/fp.2023.008","article-title":"First report of Spirometra (Eucestoda; Diphyllobothriidae) naturally occurring in a fish host","volume":"70","author":"Vettorazzi","year":"2023","journal-title":"Folia Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S0037-86821990000200009","article-title":"Human sparganosis in Ecuador: Report of a case in the province of Esmeraldas","volume":"23","author":"Guderian","year":"1990","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Martinez-Hernandez, F., Sanchez-Aguillon, F., Martinez-Oca\u00f1a, J., Gonzalez-Arenas, N.R., Romero-Valdovinos, M., Lopez-Escamilla, E., Maravilla, P., and Villalobos, G. (2023). Genetic Variability of the Internal Transcribed Spacer and Pyruvate: Ferredoxin Oxidoreductase Partial Gene of Trichomonas vaginalis from Female Patients. Microorganisms, 11.","DOI":"10.3390\/microorganisms11092240"},{"key":"ref_13","unstructured":"(2026, March 13). How to Calculate Sample Size: Ensure Sampling is Correct. Available online: https:\/\/www.qualtrics.com\/es\/gestion-de-la-experiencia\/investigacion\/calcular-tomano-muestra\/."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Milstein, M.S., Shaffer, C.A., Suse, P., Marawanaru, A., Heinrich, D.A., Larsen, P.A., and Wolf, T.M. (2022). A mixed-methods approach to understanding domestic dog health and disease transmission risk in an indigenous reserve in Guyana, South America. PLoS Neglected Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010469"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Liu, W., Gong, T., Chen, S., Liu, Q., Zhou, H., He, J., Wu, Y., Li, F., and Liu, Y. (2022). Epidemiology, diagnosis, and prevention of sparganosis in Asia. Animals, 12.","DOI":"10.3390\/ani12121578"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6155741","DOI":"10.1155\/2023\/6155741","article-title":"Prevalence of gastrointestinal helminths of dogs and associated factors in Hawassa city of Sidama region, Ethiopia","volume":"2023","author":"Dubie","year":"2023","journal-title":"J. Parasitol. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"346","DOI":"10.7705\/biomedica.v25i3.1359","article-title":"Prevalence of intestinal helminths in dogs from Quind\u00edo province","volume":"25","author":"Giraldo","year":"2005","journal-title":"BioMedica"},{"key":"ref_18","unstructured":"Girard, R. (2014). Parasitology Manual, Methods for Primary Health Care Laboratories, PAHO: National Autonomous University of Honduras. [3rd ed.]."},{"key":"ref_19","unstructured":"Bowman, G.D. (2024). Parasitology for Veterinarians, Elsevier. [11th ed.]."},{"key":"ref_20","first-page":"e947069","article-title":"Identification of Ancylostoma caninum in Domestic Dogs from Ecuador via Various Techniques","volume":"31","author":"Andrada","year":"2025","journal-title":"Med. Sci. Monit."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/molbev\/msp259","article-title":"SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building","volume":"27","author":"Gouy","year":"2010","journal-title":"Mol. Biol. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/biomet\/80.1.27","article-title":"Bias reduction of maximum likelihood estimates","volume":"80","author":"Firth","year":"1993","journal-title":"Biometrika"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1002\/sim.1047","article-title":"A solution to the problem of separation in logistic regression","volume":"21","author":"Heinze","year":"2002","journal-title":"Stat. Med."},{"key":"ref_25","unstructured":"R Core Team (2026). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_26","unstructured":"Heinze, G., Ploner, M., Dunkler, D., Southworth, H., Jiricka, L., and Steiner, G. (2026, March 23). logistf: Firth\u2019s Bias-Reduced Logistic Regression, R Package Version 1.26.1. Available online: https:\/\/cran.r-project.org\/web\/packages\/logistf."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"\u0160iki\u0107, A., Gagovi\u0107, E., Rojas, A., Sindi\u010di\u0107, M., \u017dili\u0107, D.J., Naletli\u0107, \u0160., Bali\u0107, D., Hod\u017ei\u0107, A., and Beck, R. (2025). First molecular identification of Spirometra mansoni in the golden jackal (Canis aureus) in Croatia. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1629099"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.3201\/eid2811.220529","article-title":"Presence of Spirometra mansoni, causative agent of sparganosis, in South America","volume":"28","author":"Brabec","year":"2022","journal-title":"Emerg. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102493","DOI":"10.1016\/j.parint.2021.102493","article-title":"Morphological observation and molecular phylogeny of Spirometra decipiens complex 1 (Cestoda: Diphyllobothriidae) found in cat from Chile","volume":"87","author":"Fredes","year":"2022","journal-title":"Parasitol. Int."},{"key":"ref_30","first-page":"e943931","article-title":"Ecoepidemiology of Ancylostoma spp. in urban-marginal and rural sectors of the ecuadorian coast and prevalence of cutaneous larvae migrans","volume":"30","author":"Coello","year":"2025","journal-title":"Med. Sci. Monit."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Menconi, V., Lazzaro, V.E., Bertola, M., Guardone, L., Mazzucato, V., Prearo, M., Bilska-Zajac, E., Cortinovis, L., Manfrin, A., and Arcangeli, G. (2023). The occurrence of freshwater fish-borne zoonotic helminths in Italy and neighbouring countries: A systematic review. Animals, 13.","DOI":"10.3390\/ani13243793"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zendejas-Heredia, P., Colella, V., Huggins, L., Schaper, R., Schunack, B., and Traub, R. (2023). An Integrated Coproscopic and Molecular Method Provides Insights into the Epidemiology of Zoonotic Intestinal Helminths of Dogs across Cambodia. Transbound. Emerg. Dis., 2001871.","DOI":"10.1155\/2023\/2001871"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1292\/jvms.23-0475","article-title":"Molecular identification of Spirometra infections in companion animals and wildlife in Japan","volume":"86","author":"Yamasaki","year":"2024","journal-title":"J. Vet. Med. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.pt.2026.01.001","article-title":"\u00a0Spirometra mansoni","volume":"42","author":"Rojas","year":"2026","journal-title":"Trends Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.ijid.2015.03.014","article-title":"Eosinophilic pleural effusion due to Spirometra mansoni spargana: A case report and review of the literature","volume":"34","author":"Lin","year":"2015","journal-title":"Int. J. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"874","DOI":"10.4269\/ajtmh.16-0211","article-title":"Seroprevalence of Sparganosis in Rural Communities of Northern Tanzania","volume":"95","author":"Kavana","year":"2016","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Tan, X., Zeng, Y., Lu, S., Abuzeid, A.M., Meng, Q., Zou, Z., Fan, K., and Liu, W. (2026). The Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Spirometra mansoni in Dogs. Vet. Sci., 13.","DOI":"10.3390\/vetsci13010066"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.4269\/ajtmh.19-0456","article-title":"Case report: Human subcutaneous sparganosis in a Thai migrant","volume":"101","author":"Muigg","year":"2019","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4132\/jptm.2025.10.14","article-title":"Revisiting human sparganosis: A pathologic review from a single institution","volume":"60","author":"Yim","year":"2026","journal-title":"J. Pathol. Transl. Med."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/13\/2049\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T09:16:47Z","timestamp":1783070207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/13\/2049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,3]]},"references-count":39,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16132049"],"URL":"https:\/\/doi.org\/10.3390\/ani16132049","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,3]]}}}