{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T09:05:04Z","timestamp":1784019904194,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1300804-2"],"award-info":[{"award-number":["2023YFD1300804-2"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Henan Province high-level talent internationalization training project","award":["GCC2025033"],"award-info":[{"award-number":["GCC2025033"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study assessed the effects of adding slightly acidic electrolyzed water (SAEW) to drinking water (0.3 and 0.6 mg\/L) for 15 consecutive days on the growth performance, digestive enzyme activity, amino acid transporter gene expression, antioxidant activity, and immune function in weaned piglets. Using a randomized complete block design with pen as the experimental unit, 144 healthy weaned piglets were randomly assigned to three groups with four replicate pens per group according to weight and sex. During the entire experimental period, the average daily gain and average daily feed intake of the SAEW groups were significantly higher than those of the control (CON) group (p &lt; 0.05). As compared to the CON group, the levels of \u03b1-amylase, \u03b2-amylase, lipase, and proteases in the duodenum increased in both SAEW groups (p &lt; 0.05). Furthermore, 0.6 mg\/L SAEW positively affected the mRNA levels of CAT1, PepT1, CAT2, EAAC1, rBAT, b0,+ AT, and 4F2hc in the jejunum and duodenum (p &lt; 0.05). Regarding immune function, SAEW significantly decreased intestinal secretory immunoglobulin A levels in a dose-dependent manner (p &lt; 0.05), while no significant changes were observed in serum immunoglobulin concentrations between the SAEW groups and the CON group. In terms of antioxidant status, SAEW significantly increased superoxide dismutase and glutathione peroxidase activities, but also increased malondialdehyde content in a dose-dependent manner. Despite the stronger promotion of intestinal amino acid transporters under 0.6 mg\/L SAEW, this advantage failed to generate extra growth benefits alongside elevated oxidative damage. In conclusion, both tested SAEW concentrations exert positive effects on piglet growth and digestion; comprehensively balancing production benefits and redox safety, 0.3 mg\/L SAEW is the optimal drinking water dosage for weaned piglets.<\/jats:p>","DOI":"10.3390\/ani16142124","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:40:09Z","timestamp":1783525209000},"page":"2124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Drinking Slightly Acidic Electrolyzed Water Promotes Growth Performance, Improves Digestion, and Modulates Antioxidant Status in Weaned Piglets"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7122-6523","authenticated-orcid":false,"given":"Zhifang","family":"Shi","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"},{"name":"Henan Engineering Research Center on Animal Healthy Environment and Intelligent Equipment, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zike","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiting","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuilin","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxuan","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangying","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310027, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450053, China"},{"name":"Henan Engineering Research Center on Animal Healthy Environment and Intelligent Equipment, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1071\/AN20484","article-title":"Water quality and management in the Australian pig industry","volume":"61","author":"Louise","year":"2021","journal-title":"Anim. Prod. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.cofs.2019.01.004","article-title":"Strategies for the safety management of fresh produce from farm to fork","volume":"27","author":"Campagnoli","year":"2019","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Helder, M., Koen, R.D., Evelyne, M., Els, C.V., and Jeroen, D. (2019). Limited association between disinfectant use and either antibiotic or disinfectant susceptibility of Escherichia coli in both poultry and pig husbandry. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2044-0"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1136\/vr.105495","article-title":"Oral group medication in pig production: Characterising medicated feed and drinking water systems","volume":"185","author":"Femke","year":"2019","journal-title":"Vet. Rec."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"B\u00f6ger, R., Rohn, K., Kemper, N., and Schulz, J. (2020). Sodium hypochlorite treatment: The impact on bacteria and endotoxin concentrations in drinking water pipes of a pig nursery. Agriculture, 10.","DOI":"10.3390\/agriculture10030086"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1017\/S1751731119001903","article-title":"Water medication of growing pigs: Sources of between-animal variability in systemic exposure to antimicrobials","volume":"13","author":"Little","year":"2019","journal-title":"Animal"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Friederike, H., Simon, J., Gerd, H., Jessica, M., Manfred, K., Nicole, K., Jochen, S., and Mateus-Vargas, R.H. (2022). Innovative perspectives on biofilm interactions in poultry drinking water systems and veterinary antibiotics used worldwide. Antibiotics, 11.","DOI":"10.3390\/antibiotics11010077"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3","DOI":"10.21303\/2504-5695.2019.00959","article-title":"Establishment of the efficiency of animal breeding premises disinfection by modern disinfectants","volume":"4","author":"Paliy","year":"2019","journal-title":"EUREKA Life Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1021\/acs.est.0c06254","article-title":"Electrochemical disinfection in water and wastewater treatment: Identifying impacts of water quality and operating conditions on performance","volume":"55","author":"Hand","year":"2021","journal-title":"Environ. Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100694","DOI":"10.1016\/j.coelec.2021.100694","article-title":"Electrochemical disinfection\u2013State of the art and tendencies","volume":"28","author":"Henry","year":"2021","journal-title":"Curr. Opin. Electrochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/1541-4337.12200","article-title":"Electrolyzed water as a novel sanitizer in the food industry: Current trends and future perspectives","volume":"15","author":"Rahman","year":"2016","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102228","DOI":"10.1016\/j.jwpe.2021.102228","article-title":"Electrolyzed water as a disinfectant: A systematic review of factors affecting the production and efficiency of hypochlorous acid","volume":"43","author":"Ampiaw","year":"2021","journal-title":"J. Water Process Eng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"117166","DOI":"10.1016\/j.lwt.2024.117166","article-title":"Construction of a tertiary model and uncertainty analysis for the effect of time, temperature, available chlorine concentration of slightly acidic electrolyzed water on salmonella enteritidis and background total bacteria counts on chicken","volume":"214","author":"Zang","year":"2024","journal-title":"LWT"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.jfoodeng.2012.07.011","article-title":"Stability of low concentration electrolyzed water and its sanitization potential against foodborne pathogens","volume":"113","author":"Rahman","year":"2012","journal-title":"J. Food Eng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.bbrc.2022.01.013","article-title":"Antibacterial activity and mechanism of slightly acidic electrolyzed water against Shewanella putrefaciens and Staphylococcus saprophytic","volume":"592","author":"Lin","year":"2022","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_16","unstructured":"Maksim, R., Kanza, S., Adnan, K., Ur, R.S.J., Nimra, S., Anastasia, S., Mars, K., Andrey, D., Yury, A., and Muthu, T. (2022). Application of electrolyzed water in the food industry: A review. Appl. Sci., 12."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.cofs.2021.03.004","article-title":"Recent advances on research of electrolyzed water and its applications","volume":"41","author":"Zhao","year":"2021","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Pianpian, Y., Ramachandran, C., Hee, K.J., and Hwan, D.O. (2021). Research trends on the application of electrolyzed water in food preservation and sanitation. Processes, 9.","DOI":"10.3390\/pr9122240"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Farah, R.I., and Ali, S.N.A.-H. (2021). Electrolyzed water generated on-site as a promising disinfectant in the dental office during the COVID-19 pandemic. Front. Public Health, 9.","DOI":"10.3389\/fpubh.2021.629142"},{"key":"ref_20","first-page":"11","article-title":"Nasopharyngeal and oropharyngeal rinses with neutral electrolyzed water prevents COVID-19 in front-line health professionals: A randomized, open-label, controlled trial in a general hospital in Mexico City","volume":"16","year":"2022","journal-title":"Biomed. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5932","DOI":"10.3382\/ps\/pez373","article-title":"Efficacy of slightly acidic electrolyzed water on the microbial safety and shelf life of shelled eggs","volume":"98","author":"Zang","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1080\/10962247.2019.1567622","article-title":"Optimization of a wet scrubber with electrolyzed water spray\u2014Part II: Airborne culturable bacteria removal","volume":"69","author":"Li","year":"2019","journal-title":"J. Air Waste Manag. Assoc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104012","DOI":"10.1016\/j.psj.2024.104012","article-title":"Effect of combined electrolyzed reduced water and slightly acidic electrolyzed water spraying on the control of Salmonella, eggshell quality, and shelf life of eggs during storage","volume":"103","author":"Tu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_24","first-page":"229","article-title":"Airborne particulate matter and culturable bacteria reduction from spraying slightly acidic electrolyzed water in an experimental aviary laying-hen housing chamber","volume":"57","author":"Zheng","year":"2014","journal-title":"Trans. ASABE"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101643","DOI":"10.1016\/j.psj.2021.101643","article-title":"Slightly acidic electrolyzed water as an alternative disinfection technique for hatching eggs","volume":"101","author":"Liu","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1258\/la.2011.010122","article-title":"Effects of slightly acidic electrolysed drinking water on mice","volume":"45","author":"Inagaki","year":"2011","journal-title":"Lab. Anim."},{"key":"ref_27","first-page":"27","article-title":"Effects of drinking slightly acidic electrolyzed water on intestinal microbes and immune function of broilers","volume":"48","author":"Ji","year":"2020","journal-title":"J. Northwest AF Univ."},{"key":"ref_28","first-page":"122","article-title":"Effects of drinking slightly acidic electrolyzed water on growth performance and behavior of Ross 308 broilers","volume":"56","author":"Ji","year":"2020","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1080\/09540105.2017.1360254","article-title":"Effects of microencapsulated Lactobacillus plantarum and fructooligosaccharide on growth performance, blood immune parameters, and intestinal morphology in weaned piglets","volume":"29","author":"Wang","year":"2018","journal-title":"Food Agric. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, Y., Zhang, T., Jia, F., Li, H., Sun, M., Fu, Z., Zhou, H., Guo, W., and Gao, Y. (2023). Effects of ferulic acid on growth performance and intestinal oxidation indexes of Jilin white geese under lipopolysaccharide-induced oxidative stress. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0291955"},{"key":"ref_32","first-page":"230","article-title":"Experiment on bactericidal efficacy in drinking system using slightly acidic electrolyzed water in large-scale poultry houses","volume":"33","author":"Wang","year":"2017","journal-title":"Trans. Chin. Soc. Agric. Eng."},{"key":"ref_33","first-page":"113","article-title":"Study on the effects of slightly acidic electrolyzed water on the intestinal microflora of laying hens","volume":"23","author":"Wang","year":"2018","journal-title":"J. China Agric. Univ."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hao, X., Xie, D., Jiang, D., Zhu, L., Shen, L., Gan, M., and Bai, L. (2023). Effect of Slightly Acidic Electrolyzed Water on Growth, Diarrhea and Intestinal Bacteria of Newly Weaned Piglets. Genes, 14.","DOI":"10.3390\/genes14071398"},{"key":"ref_35","first-page":"184","article-title":"Effect of administration of organic acids in drinking water on faecal shedding of E. coli, performance parameters and health in nursery pigs","volume":"188","author":"Dewulf","year":"2010","journal-title":"Vet. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"104311","DOI":"10.1016\/j.livsci.2020.104311","article-title":"Benzoic acid and essential oils modify the cecum microbiota composition in weaned piglets and improve growth performance in finishing pigs","volume":"242","author":"Filipe","year":"2020","journal-title":"Livest. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"114","DOI":"10.2174\/138920372002181113112335","article-title":"Bioavailability and Turnover of Proteins in Mammals","volume":"20","author":"Ma","year":"2019","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e22706","DOI":"10.1002\/jbt.22706","article-title":"Effect of fumonisin B1 on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells","volume":"35","author":"Zhigang","year":"2021","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1369\/0022155417749828","article-title":"Expression of glutamate transporters in mouse liver, kidney, and intestine","volume":"66","author":"Hu","year":"2018","journal-title":"J. Histochem. Cytochem."},{"key":"ref_40","first-page":"287","article-title":"First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid","volume":"54","author":"Ighodaro","year":"2018","journal-title":"Alex. J. Med."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Munteanu, I.G., and Apetrei, C. (2021). Analytical methods used in determining antioxidant activity: A review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073380"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2124\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T08:14:33Z","timestamp":1784016873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,8]]},"references-count":41,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16142124"],"URL":"https:\/\/doi.org\/10.3390\/ani16142124","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,8]]}}}