{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:59:52Z","timestamp":1785405592750,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Guidance Funds for Local Science and Technology Development Projects","award":["25ZYJA008"],"award-info":[{"award-number":["25ZYJA008"]}]},{"name":"Key Talent Project of Gansu Province","award":["2026RCXM096"],"award-info":[{"award-number":["2026RCXM096"]}]},{"name":"The Modern Beef Yak Industry Technology System","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"The Innovation Project of Chinese Academy of Agricultural Sciences","award":["25-LIHPS-01"],"award-info":[{"award-number":["25-LIHPS-01"]}]},{"award":["25-LIHPS-01"],"award-info":[{"award-number":["25-LIHPS-01"]}],"id":[{"id":"https:\/\/ror.org\/0313jb750","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Yak is a distinctive domestic animal adapted to the Qinghai\u2013Tibet Plateau, and its lactation performance is restricted by extreme high-altitude conditions. Despite the growing recognition of circRNAs as important regulators of mammary gland development in other species, no study has yet systematically investigated the stage-specific circRNA expression landscapes in yak mammary glands across gestation, lactation, and non-pregnant periods, or the potential ceRNA networks through which these circRNA may participate in regulating milk synthesis and mammary cell proliferation under high-altitude conditions. This study aimed to characterize circRNA expression features and lactation regulation in yak mammary glands at the gestation, lactation, and non-pregnant stages. Total RNA sequencing was performed on mammary specimens, followed by differential screening, functional enrichment, ceRNA network construction, and qPCR verification. Numerous stage-biased circRNAs were identified, among which circNFATC2, circTRPS1 and circTAF3 were highlighted as hub candidates potentially involved in mammary cell differentiation, milk synthesis, and energy metabolism, with qPCR validating sequencing outcomes. Our results provide a comprehensive circRNA expression atlas and candidate molecular markers for understanding lactation regulation in high-altitude yaks, offering a valuable foundation for the future genetic improvement of milk production traits.<\/jats:p>","DOI":"10.3390\/ani16142173","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:55:21Z","timestamp":1783990521000},"page":"2173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of circRNA Differential Expression and ceRNA Network Construction in Yak Mammary Glands Across Different Physiological Stages"],"prefix":"10.3390","volume":"16","author":[{"given":"Zhenyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730046, China"},{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5659-4341","authenticated-orcid":false,"given":"Yongfu","family":"La","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7671-1050","authenticated-orcid":false,"given":"Pengjia","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2390-4777","authenticated-orcid":false,"given":"Xian","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Zhangye Livestock Breeding and Improvement Station of Gansu Province, Zhangye 734000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunnian","family":"Liang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104133","DOI":"10.1016\/j.tifs.2023.104133","article-title":"Nutrition and edible characteristics, origin traceability and authenticity identification of yak meat and milk: A review","volume":"139","author":"Li","year":"2023","journal-title":"Trends Food Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Li, W., Zeng, W., Zhang, Y., Ma, Z., Fang, X., Han, Y., Sun, Y., Jin, X., and Ma, L. (2023). A comparative metabolomics analysis of domestic yak (Bos grunniens) milk with human breast milk. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1207950"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/s41574-022-00742-y","article-title":"Hormonal regulation of mammary gland development and lactation","volume":"19","author":"Hannan","year":"2023","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Biswas, S.K., Banerjee, S., Baker, G.W., Kuo, C.Y., and Chowdhury, I. (2022). The Mammary Gland: Basic Structure and Molecular Signaling during Development. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073883"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"195154","DOI":"10.1016\/j.bbagrm.2026.195154","article-title":"Circular RNAs in animals: Biogenesis, function and cell fate control","volume":"1869","author":"Mohammad","year":"2026","journal-title":"Biochim. Biophys. Acta-Gene Regul. Mech."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/s42003-024-07383-z","article-title":"Mechanisms and therapeutic implications of gene expression regulation by circRNA-protein interactions in cancer","volume":"8","author":"Zhang","year":"2025","journal-title":"Commun. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1021\/acs.jafc.1c07358","article-title":"Circular RNA Profiling Identifies Novel circPPARA that Promotes Intramuscular Fat Deposition in Pigs","volume":"70","author":"Li","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e3153","DOI":"10.1038\/cddis.2017.541","article-title":"Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p","volume":"8","author":"Wei","year":"2017","journal-title":"Cell Death Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2186","DOI":"10.1016\/j.ygeno.2019.12.014","article-title":"Identification and characterization of circular RNA in lactating mammary glands from two breeds of sheep with different milk production profiles using RNA-Seq","volume":"112","author":"Hao","year":"2020","journal-title":"Genomics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4750","DOI":"10.3168\/jds.2015-10381","article-title":"Circular RNA of cattle casein genes are highly expressed in bovine mammary gland","volume":"99","author":"Zhang","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s00203-025-04269-4","article-title":"Profile of circular RNAs in bovine mammary tissues infected with Staphylococcus aureus","volume":"207","author":"Zhou","year":"2025","journal-title":"Arch. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e107","DOI":"10.1002\/imt2.107","article-title":"Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp","volume":"2","author":"Chen","year":"2023","journal-title":"Imeta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows-Wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13059-014-0571-3","article-title":"CIRI: An efficient and unbiased algorithm for de novo circular RNA identification","volume":"16","author":"Gao","year":"2015","journal-title":"Genome Biol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Shang, F., Wang, Y., Ma, R., Di, Z., Wu, Z., Hai, E., Rong, Y., Pan, J., Liang, L., and Wang, Z. (2021). Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.678825"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_18","first-page":"100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"W316","DOI":"10.1093\/nar\/gkr483","article-title":"KOBAS 2.0: A web server for annotation and identification of enriched pathways and diseases","volume":"39","author":"Xie","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chen, W., Gu, X., Lv, X., Cao, X., Yuan, Z., Wang, S., and Sun, W. (2022). Non-coding transcriptomic profiles in the sheep mammary gland during different lactation periods. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.983562"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Liang, Y., Gao, Q., Wang, H., Guo, M., Arbab, A.A.I., Nazar, M., Li, M., Yang, Z., Karrow, N.A., and Mao, Y. (2022). Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation. Biomolecules, 12.","DOI":"10.3390\/biom12030478"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Feng, X., Cai, Z., Mu, T., Yu, B., Wang, Y., Ma, R., Liu, J., Wang, C., Zhang, J., and Gu, Y. (2022). CircRNA screening and ceRNA network construction for milk fat metabolism in dairy cows. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.995629"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/s40164-023-00451-w","article-title":"New insight into circRNAs: Characterization, strategies, and biomedical applications","volume":"12","author":"Feng","year":"2023","journal-title":"Exp. Hematol. Oncol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e10032","DOI":"10.7717\/peerj.10032","article-title":"The mechanism and detection of alternative splicing events in circular RNAs","volume":"8","author":"Li","year":"2020","journal-title":"PeerJ"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yu, D., Xin, L., Qing, X., Hao, Z., Yong, W., Jiangjiang, Z., and Yaqiu, L. (2023). Key circRNAs from goat: Discovery, integrated regulatory network and their putative roles in the differentiation of intramuscular adipocytes. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09141-7"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.molcel.2015.03.027","article-title":"Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed","volume":"58","author":"Stottmeister","year":"2015","journal-title":"Mol. Cell"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Xia, W., Liu, Y., Loor, J.J., Bionaz, M., and Jiang, M. (2023). Dynamic Profile of the Yak Mammary Transcriptome during the Lactation Cycle. Animals, 13.","DOI":"10.3390\/ani13101710"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"31783","DOI":"10.1021\/acs.jafc.5c08723","article-title":"Sestrin 2 Regulates Milk Protein Synthesis through a LAT1\/mTOR Pathway","volume":"73","author":"Ke","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"250560","DOI":"10.5713\/ab.250560","article-title":"Time-resolved transcriptomic profiling of mammary gland tissue during ductal morphogenesis, lactation activation, and involution in sows","volume":"39","author":"Peng","year":"2026","journal-title":"Anim. Biosci."},{"key":"ref_31","unstructured":"Skinner, M.K. (2026). The Cell Biology of the Lactating Mammary Epithelium. Encyclopedia of Reproduction, Academic Press. [3rd ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10377","DOI":"10.3168\/jds.2025-26639","article-title":"Integrating transcriptomic and metabolomic profiles of primiparous Holstein cows across multiple lactation periods reveals the regulatory mechanism underlying milk component traits","volume":"108","author":"Fu","year":"2025","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"101302","DOI":"10.1016\/j.cofs.2025.101302","article-title":"Milk fat globule membrane ingredients and their potential applications for human health and performance","volume":"63","author":"McCarthy","year":"2025","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/s41580-023-00683-y","article-title":"Energy-driven genome regulation by ATP-dependent chromatin remodellers","volume":"25","author":"Eustermann","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1111\/jpn.14122","article-title":"Differences in Milk Production Efficiency and Physiology Between Lactating Holstein Cows and Yaks Born and Kept at About 4000 m of Altitude When Fed the Same Diet","volume":"109","author":"Zhang","year":"2025","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1093\/humupd\/dmaa008","article-title":"Brain-derived neurotrophic factor (BDNF) expression and function in the mammalian reproductive Tract","volume":"26","author":"Chow","year":"2020","journal-title":"Hum. Reprod. Update"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4758","DOI":"10.1073\/pnas.1909731117","article-title":"Occludin protects secretory cells from ER stress by facilitating SNARE-dependent apical protein exocytosis","volume":"117","author":"Zhou","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"112538","DOI":"10.1016\/j.isci.2025.112538","article-title":"Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress","volume":"28","author":"Vuong","year":"2025","journal-title":"iScience"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"120659","DOI":"10.1016\/j.jep.2025.120659","article-title":"Salidroside improves hypoxia-induced milk synthesis disorder and endoplasmic reticulum stress via AKT\/mTOR signaling in bovine mammary epithelial cells","volume":"355","author":"Liu","year":"2026","journal-title":"J. Ethnopharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6587","DOI":"10.3168\/jds.2025-26358","article-title":"Invited review: Interactions between prolactin and local regulation of the mammary gland","volume":"108","author":"Lacasse","year":"2025","journal-title":"J. Dairy Sci."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhou, Q., Geng, Z., Lian, S., Wang, J., and Wu, R. (2025). ATR Deficiency Impairs DNA Damage Repair and Accelerates Cellular Senescence in Bovine Mammary Epithelial Cells, Leading to Lactation Dysfunction. Animals, 15.","DOI":"10.3390\/ani15101419"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"114090","DOI":"10.1016\/j.yexcr.2024.114090","article-title":"Regulatory roles of dopamine D2 receptor in milk protein production and apoptosis in mammary epithelial cells","volume":"439","author":"Han","year":"2024","journal-title":"Exp. Cell Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Feng, X., Tong, L., Ma, L., Mu, T., Yu, B., Ma, R., Li, J., Wang, C., Zhang, J., and Gu, Y. (2024). Mining key circRNA-associated-ceRNA networks for milk fat metabolism in cows with varying milk fat percentages. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10252-y"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1186\/s40001-025-02720-2","article-title":"Mediator complex: Update of key insights into transcriptional regulation of ancestral framework and its role in cardiovascular diseases","volume":"30","author":"Schiano","year":"2025","journal-title":"Eur. J. Med. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/s41419-024-06942-w","article-title":"The feedback loop between MTA1 and MTA3\/TRIM21 modulates stemness of breast cancer in response to estrogen","volume":"15","author":"Zhang","year":"2024","journal-title":"Cell Death Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"102656","DOI":"10.1016\/j.plefa.2024.102656","article-title":"Impact of polyunsaturated fatty acids during and pregnancy and lactation: A comprehensive review","volume":"203","author":"Mercado","year":"2024","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s12711-024-00890-x","article-title":"A common regulatory haplotype doubles lactoferrin concentration in milk","volume":"56","author":"Lopdell","year":"2024","journal-title":"Genet. Sel. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"15155","DOI":"10.1038\/s41598-017-15508-0","article-title":"Characterization of mammary-specific disruptions for Tph1 and Lrp5 during murine lactation","volume":"7","author":"Weaver","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zhou, X., Ullah, A., Shi, L., Dou, M., Wang, C., Khan, M.Z., Wang, C., and Zhang, X. (2025). Molecular Regulatory Mechanisms of Mammary Gland Development: A Review. Animals, 15.","DOI":"10.3390\/ani15233480"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"21678","DOI":"10.1038\/s41598-024-72681-9","article-title":"Characterization of Shy1, the Schizosaccharomyces pombe homolog of human SURF1","volume":"14","author":"Luo","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3467","DOI":"10.3168\/jds.2011-4163","article-title":"Transcript abundance of amino acid transporters, \u03b2-casein, and \u03b1-lactalbumin in mammary tissue of periparturient, lactating, and postweaned sows","volume":"94","author":"Manjarin","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/j.1472-8206.2009.00760.x","article-title":"Lactation stage-dependent expression of transporters in rat whole mammary gland and primary mammary epithelial organoids","volume":"24","author":"Gilchrist","year":"2010","journal-title":"Fundam. Clin. Pharmacol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Li, G., Li, D., Wang, T., and He, S. (2021). Pyrimidine Biosynthetic Enzyme CAD: Its Function, Regulation, and Diagnostic Potential. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910253"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2173\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T01:06:24Z","timestamp":1783991184000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,13]]},"references-count":53,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16142173"],"URL":"https:\/\/doi.org\/10.3390\/ani16142173","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,13]]}}}