{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:42:54Z","timestamp":1785404574442,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273152"],"award-info":[{"award-number":["32273152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32330110"],"award-info":[{"award-number":["32330110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32273152"],"award-info":[{"award-number":["32273152"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32330110"],"award-info":[{"award-number":["32330110"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Zhanjiang Innovation and Entrepreneurship Team Cultivation \u201cPilot Program\u201d Project","award":["211207157080995"],"award-info":[{"award-number":["211207157080995"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The intensive culture of largemouth bass (Micropterus salmoides) under high-protein feeding conditions is frequently associated with ammonia nitrogen stress, which adversely affects hepatic health. L-Ornithine L-aspartate (LOLA) has been proposed as a regulator of nitrogen metabolism. However, its effects in aquafeeds remain unclear. Five experimental diets (LOLA0, LOLA3, LOLA6, LOLA9, LOLA12) containing graded levels of LOLA (0%, 0.03%, 0.06%, 0.09%, 0.12%) were formulated, along with a diet supplemented with 0.09% L-ornithine + L-aspartate (OA9). Each diet was fed to fish reared in triplicate cages (30 fish per cage, initial body weight: 7.38 \u00b1 0.02 g) for 10 weeks. No significant effects were observed on growth performance (weight gain, specific growth rate) or feed utilization (feed conversion rate, protein efficiency ratio) among the different LOLA treatments (p &gt; 0.05). Nevertheless, second-order polynomial regression analysis revealed that dietary inclusion of 0.05\u20130.08% LOLA significantly reduced plasma ammonia and urea nitrogen levels, decreased hepatic aspartate aminotransferase and glutamate dehydrogenase activities, as well as plasma adenosine monophosphate deaminase activity, activated the hepatic TOR-S6K1-related markers, and reduced mortality under ammonia nitrogen stress (p &lt; 0.05). Furthermore, supplementation with 0.07\u20130.08% LOLA significantly enhanced glutathione peroxidase and superoxide dismutase activities in both serum and liver, as well as serum levels of lysozyme and immunoglobulin M. Additionally, compared with the OA9 group, the LOLA9 group showed more pronounced changes in TOR-S6K1-related markers, ammonia load, antioxidant and immune parameters. Overall, while no significant growth enhancement was observed, these biochemical and physiological modifications suggest that LOLA supplementation is associated with alterations in nitrogen metabolism, antioxidant, and immune indices in largemouth bass, but further studies are needed to confirm the functional significance of these changes.<\/jats:p>","DOI":"10.3390\/ani16142190","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:05:58Z","timestamp":1784077558000},"page":"2190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary L-Ornithine L-Aspartate Alleviates Ammonia Load and Improves Hepatic Health in Largemouth Bass (Micropterus salmoides)"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1856-8545","authenticated-orcid":false,"given":"Lizhen","family":"Jia","sequence":"first","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangzhi","family":"Ma","sequence":"additional","affiliation":[{"name":"Anhui Wanhe Jial Biotechnology Co., Ltd., Chuzhou 239000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2461-2533","authenticated-orcid":false,"given":"Shiwei","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beiping","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8753-8318","authenticated-orcid":false,"given":"Shuyan","family":"Chi","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2102-2092","authenticated-orcid":false,"given":"Junming","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"30058","DOI":"10.1038\/s41598-024-80904-2","article-title":"Whole Genome Resequencing Reveals the Correlation between Selection Signatures and Adaptability of Micropterus Salmoides to Artificial Fed","volume":"14","author":"Zhu","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_2","unstructured":"BFFA (Bureau of Fisheries and Fishery Administration, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China) (2025). China Fishery Statistics Yearbook 2025, China Agriculture Press."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mes, W., Kersten, P., Maas, R.M., Eding, E.H., Jetten, M.S.M., Siepel, H., L\u00fccker, S., Gorissen, M., and Van Kessel, M.A.H.J. (2023). Effects of Demand-Feeding and Dietary Protein Level on Nitrogen Metabolism and Symbiont Dinitrogen Gas Production of Common Carp (Cyprinus carpio, L.). Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1111404"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0044-8486(96)01356-7","article-title":"Effects of Dietary Protein Level on Second Year Growth and Water Quality for Largemouth Bass (Micropterus salmoides) Raised in Ponds","volume":"145","author":"Tidwell","year":"1996","journal-title":"Aquaculture"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ip, Y., and Chew, S.F. (2010). Ammonia Production, Excretion, Toxicity, and Defense in Fish: A Review. Front. Physiol., 1.","DOI":"10.3389\/fphys.2010.00134"},{"key":"ref_6","first-page":"112","article-title":"Gill Metabolic and Osmoregulatory Responses of Juvenile Atlantic Cod (Gadus morhua) to Chronic Ammonia Exposure","volume":"24","author":"Savoie","year":"2008","journal-title":"J. Appl. Ichthyol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Li, Y., Yang, R., He, M., Su, J., and Liu, L. (2025). Chronic Ammonia Stress in Chinese Perch (Siniperca chuatsi): Oxidative Response, Nitrogen Metabolism, and Multi-Enzyme-Mediated Molecular Detoxification Defense Mechanisms. Antioxidants, 14.","DOI":"10.3390\/antiox14070768"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pu, D., Wang, Z., Zheng, J., Li, P., Wei, X., Li, D., Gao, L., Zhou, L., and Wang, Y. (2024). Effects of Ammonia Stress on Liver Tissue Structure, Enzyme Activities, and Metabolome of Juvenile Largemouth Bass Micropterus salmoides. Metabolites, 14.","DOI":"10.3390\/metabo14120649"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Erdal, R., Birsoy, K., and Unlu, G. (2025). Amino Acid Metabolism in Liver Mitochondria: From Homeostasis to Disease. Metabolites, 15.","DOI":"10.3390\/metabo15070446"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1006\/abbi.1997.0522","article-title":"Nitrogen Excretion and Expression of Carbamoyl-Phosphate Synthetase III Activity and mRNA in Extrahepatic Tissues of Largemouth Bass (Micropterus salmoides)","volume":"350","author":"Kong","year":"1998","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zou, J., Hu, P., Wang, M., Chen, Z., Wang, H., Guo, X., Gao, J., and Wang, Q. (2023). Liver Injury and Metabolic Dysregulation in Largemouth Bass (Micropterus salmoides) after Ammonia Exposure. Metabolites, 13.","DOI":"10.3390\/metabo13020274"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1023\/A:1021934607762","article-title":"Clinical Efficacy of L-Ornithine\u2013L-Aspartate in the Management of Hepatic Encephalopathy","volume":"17","author":"Kircheis","year":"2002","journal-title":"Metab. Brain Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1002\/hep.29410","article-title":"L-Ornithine L-Aspartate in Bouts of Overt Hepatic Encephalopathy","volume":"67","author":"Sidhu","year":"2018","journal-title":"Hepatology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11862","DOI":"10.1038\/s41598-024-62293-8","article-title":"Intravenous versus Oral \u2018l-Ornithine-l-Aspartate\u2019 in Overt Hepatic Encephalopathy: A Randomized Comparative Study","volume":"14","author":"Jhajharia","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1159\/000491429","article-title":"Hepatoprotection by L-Ornithine L-Aspartate in Non-Alcoholic Fatty Liver Disease","volume":"37","author":"Butterworth","year":"2019","journal-title":"Dig. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"De Freitas, L.B.R., Longo, L., Filippi-Chiela, E., De Souza, V.E.G., Behrens, L., Pereira, M.H.M., Leonhard, L.C., Zanettini, G., Pinzon, C.E., and Luchese, E. (2022). Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats. Biomolecules, 12.","DOI":"10.3390\/biom12121773"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pichon, C., Nachit, M., Gillard, J., Vande Velde, G., Lanthier, N., and Leclercq, I.A. (2022). Impact of L-Ornithine L-Aspartate on Non-Alcoholic Steatohepatitis-Associated Hyperammonemia and Muscle Alterations. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1051157"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"104487","DOI":"10.1016\/j.dci.2022.104487","article-title":"Establishment of Hyperammonemia Mode in Yellow Catfish and the Mitigation of Exogenous L-Ornithine-L-Aspartate","volume":"135","author":"Zhang","year":"2022","journal-title":"Dev. Comp. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1111\/anu.12939","article-title":"Dietary Supplementation of N-Carbamylglutamate and Effects on Growth, Intestinal Enzyme Activities, Immunological and Antioxidant Abilities of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)","volume":"25","author":"Zhao","year":"2019","journal-title":"Aquacult. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chen, J., Dong, X., Yang, Q., Chi, S., Zhang, S., Tan, B., and Deng, J. (2025). Dietary Tryptophan Requirement of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus\u2640 \u00d7 E. lanceolatus\u2642). Animals, 15.","DOI":"10.3390\/ani15010104"},{"key":"ref_21","unstructured":"AOAC (2006). Official Methods of Analysis of AOAC International, Association of Official Analytical Chemists. [18th ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ygcen.2012.08.004","article-title":"Effects of Dietary Arginine Levels and Carbohydrate-to-Lipid Ratios on mRNA Expression of Growth-Related Hormones in Largemouth Bass, Micropterus salmoides","volume":"179","author":"Chen","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.fsi.2020.10.018","article-title":"Protective Immunity of Largemouth Bass Immunized with Immersed DNA Vaccine against Largemouth Bass Ulcerative Syndrome Virus","volume":"107","author":"Jia","year":"2020","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_25","first-page":"279","article-title":"Cautionary Note about R2","volume":"39","year":"1985","journal-title":"Am. Stat."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0305-0491(94)90083-3","article-title":"Induction of Ornithine-Urea Cycle in a Freshwater Teleost, Heteropneustes Fossilis, Exposed to High Concentrations of Ammonium Chloride","volume":"108","author":"Saha","year":"1994","journal-title":"Comp. Biochem. Physiol. Part B Comp. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1079\/BJN19950025","article-title":"Metabolism of Ornithine, \u03b1-Ketoglutarate and Arginine in Isolated Perfused Rat Liver","volume":"73","author":"Cynober","year":"1995","journal-title":"Br. J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1017\/S0007114511003291","article-title":"Retinal Risks of High-Dose Ornithine Supplements: A Review","volume":"106","author":"Hayasaka","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"735491","DOI":"10.1016\/j.aquaculture.2020.735491","article-title":"Dietary Citrulline Improves Survival of Rainbow Trout Oncorhynchus mykiss Juveniles Challenged with Vibrio anguillarum","volume":"528","author":"Fauzi","year":"2020","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110632","DOI":"10.1016\/j.cbpa.2019.110632","article-title":"Supplementation of Arginine, Ornithine and Citrulline in Rainbow Trout (Oncorhynchus mykiss): Effects on Growth, Amino Acid Levels in Plasma and Gene Expression Responses in Liver Tissue","volume":"241","author":"Clark","year":"2020","journal-title":"Comp. Biochem. Physiol. A. Mol. Integr. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"102095","DOI":"10.1016\/j.aqrep.2024.102095","article-title":"Dietary Supplementation of Ornithine Improved the Growth, Immune Response and Acute NH3 Stress Tolerance of Pacific White Shrimp, Penaeus vannamei","volume":"36","author":"Qu","year":"2024","journal-title":"Aquac. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s00726-013-1500-6","article-title":"Functional Amino Acids in Nutrition and Health","volume":"45","author":"Wu","year":"2013","journal-title":"Amino Acids"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00726-015-2065-3","article-title":"Dietary Supplementation with L-Glutamate and l-Aspartate Alleviates Oxidative Stress in Weaned Piglets Challenged with Hydrogen Peroxide","volume":"48","author":"Duan","year":"2016","journal-title":"Amino Acids"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s40104-025-01266-x","article-title":"Effects of Dietary L-Glutamate and L-Aspartate Supplementation on Growth Performance, Severity of Diarrhea, Intestinal Barrier Integrity, and Fecal Microbiota of Weaned Piglets Challenged with F18 Enterotoxigenic Escherichia coli.","volume":"16","author":"Wongchanla","year":"2025","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1002\/hep.29107","article-title":"Ammonia Lowering Reverses Sarcopenia of Cirrhosis by Restoring Skeletal Muscle Proteostasis","volume":"65","author":"Kumar","year":"2017","journal-title":"Hepatology"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Zhang, X., Ma, G., Wang, B., and Guo, Y. (J. Adv. Res., 2026). L-Ornithine-L-Aspartate Enhances Protein Anabolism and Growth Performance in Broilers by Modulating the Liver-NH3\/Gln-Muscle Axis, J. Adv. Res., in press.","DOI":"10.1016\/j.jare.2026.05.045"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"102461","DOI":"10.1016\/j.aqrep.2024.102461","article-title":"Effects of Dietary Protein Levels with Cottonseed Protein Concentrate Inclusion on Growth, Feed Utilization, Liver Health and Intestinal Microbiota of Juvenile Largemouth Bass (Micropterus salmoides)","volume":"39","author":"Chen","year":"2024","journal-title":"Aquac. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"109555","DOI":"10.1016\/j.fsi.2024.109555","article-title":"Effects of Essential Amino Acids Supplementation in a Low-Protein Diet on Growth Performance, Intestinal Health and Microbiota of Juvenile Blotched Snakehead (Channa maculata)","volume":"149","author":"Fei","year":"2024","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5639","DOI":"10.1007\/s10499-024-01440-4","article-title":"A Review of Dietary Approaches for Ammonia Toxicity Remediation in Fish","volume":"32","author":"Hoseini","year":"2024","journal-title":"Aquacult. Int."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s00360-024-01561-5","article-title":"Ammonia Excretion by the Fish Gill: Discoveries and Ideas That Shaped Our Current Understanding","volume":"194","author":"Zimmer","year":"2024","journal-title":"J. Comp. Physiol. B"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/0305-0491(83)90127-X","article-title":"Aerobic and Anaerobic Ammonia Production by Fish","volume":"74","year":"1983","journal-title":"Comp. Biochem. Physiol. Part B Comp. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/337165a0","article-title":"Urea Excretion as a Strategy for Survival in a Fish Living in a Very Alkaline Environment","volume":"337","author":"Randall","year":"1989","journal-title":"Nature"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.aquaeng.2014.10.004","article-title":"Nitrogen Waste from Rainbow Trout (Oncorhynchus mykiss) with Particular Focus on Urea","volume":"65","author":"Dalsgaard","year":"2015","journal-title":"Aquac. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.mce.2015.08.013","article-title":"Metabolic, Anabolic, and Mitogenic Insulin Responses: A Tissue-Specific Perspective for Insulin Receptor Activators","volume":"415","author":"Bedinger","year":"2015","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1093\/jn\/132.1.59","article-title":"Somatotropin-Induced Amino Acid Conservation in Pigs Involves Differential Regulation of Liver and Gut Urea Cycle Enzyme Activity","volume":"132","author":"Bush","year":"2002","journal-title":"J. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110487","DOI":"10.1016\/j.cbpc.2026.110487","article-title":"Advances in Response Mechanisms of Fish to Ammonia Stress: A Review","volume":"304","author":"Yu","year":"2026","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1023\/A:1020613314572","article-title":"L-Ornithine-L-Aspartate in Experimental Portal-Systemic Encephalopathy: Therapeutic Efficacy and Mechanism of Action","volume":"13","author":"Rose","year":"1998","journal-title":"Metab. Brain Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jceh.2018.05.004","article-title":"Efficacy of L-Ornithine l-Aspartate for the Treatment of Hepatic Encephalopathy and Hyperammonemia in Cirrhosis: Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"8","author":"Butterworth","year":"2018","journal-title":"J. Clin. Exp. Hepatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"100276","DOI":"10.1016\/j.aqrep.2020.100276","article-title":"Indirect Effect of Different Dietary Protein to Energy Ratio of Bait Fish Mori Diets on Growth Performance, Body Composition, Nitrogen Metabolism and Relative AMPK & mTOR Pathway Gene Expression of Chinese Perch","volume":"16","author":"Alam","year":"2020","journal-title":"Aquac. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/0301-0082(92)90006-Z","article-title":"The Purine Nucleotide Cycle and Its Molecular Defects","volume":"39","author":"Bontemps","year":"1992","journal-title":"Prog. Neurobiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1051\/parasite\/2024065","article-title":"Biological Characteristics and Functions of a Novel Glutamate Dehydrogenase from Trichinella spiralis","volume":"31","author":"Cheng","year":"2024","journal-title":"Parasite"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S1357-2725(97)00100-3","article-title":"Long-Term Nutritional Effects on the Primary Liver and Kidney Metabolism in Rainbow Trout. Adaptive Response to Starvation and a High-Protein, Carbohydrate-Free Diet on Glutamate Dehydrogenase and Alanine Aminotransferase Kinetics","volume":"30","year":"1998","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1007\/s00580-020-03175-z","article-title":"The Effects of Treatments with Multistrain Probiotics on Serum Aminotransferases, AST-ALT Ratio, Body Weight and Survivability Scores of Rats Experimentally Infected with Trypanosoma brucei","volume":"29","author":"Okolo","year":"2020","journal-title":"Comp. Clin. Pathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"687690","DOI":"10.1155\/2015\/687690","article-title":"Effects of Soothing Liver and Invigorating Spleen Recipes on the IKK \u03b2 -NF- \u03ba B Signaling Pathway in Kupffer Cells of Nonalcoholic Steatohepatitis Rats","volume":"2015","author":"Gong","year":"2015","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0044-8486(91)90205-L","article-title":"Changes with Different Diets in Plasma Enzymes (GOT, GPT, LDH, ALP) and Plasma Lipids (Cholesterol, Triglycerides) of Sea-Bass (Dicentrarchus labrax)","volume":"93","author":"Lemaire","year":"1991","journal-title":"Aquaculture"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.fgb.2012.11.007","article-title":"\u03b3-Glutamyltransferases (GGT) in Colletotrichum graminicola: mRNA and Enzyme Activity, and Evidence That CgGGT1 Allows Glutathione Utilization during Nitrogen Deficiency","volume":"51","author":"Bello","year":"2013","journal-title":"Fungal Genet. Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/s41580-019-0199-y","article-title":"mTOR at the Nexus of Nutrition, Growth, Ageing and Disease","volume":"21","author":"Liu","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1152\/physrev.00026.2020","article-title":"Regulation and Metabolic Functions of mTORC1 and mTORC2","volume":"101","author":"Szwed","year":"2021","journal-title":"Physiol. Rev."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Adams-Brown, S.E., and Reid, K.Z. (2025). The Central FacilitaTOR: Coordinating Transcription and Translation in Eukaryotes. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26072845"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"36553","DOI":"10.1074\/jbc.M404559200","article-title":"Preservation of Liver Protein Synthesis during Dietary Leucine Deprivation Occurs at the Expense of Skeletal Muscle Mass in Mice Deleted for eIF2 Kinase GCN2","volume":"279","author":"Anthony","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.tibs.2013.01.004","article-title":"Nutrient Signaling to mTOR and Cell Growth","volume":"38","author":"Jewell","year":"2013","journal-title":"Trends Biochem. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.aquaculture.2015.12.019","article-title":"Dietary Methionine Level Influences Growth and Lipid Metabolism via GCN2 Pathway in Cobia (Rachycentron canadum)","volume":"454","author":"Wang","year":"2016","journal-title":"Aquaculture"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.fsi.2021.10.039","article-title":"Effects of Sodium Butyrate Nanoparticles on the Hemato-Immunological Indices, Hepatic Antioxidant Capacity, and Gene Expression Responses in Oreochromis niloticus","volume":"119","author":"Hendam","year":"2021","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1111\/faf.12215","article-title":"A Comparative and Evolutionary Approach to Oxidative Stress in Fish: A Review","volume":"18","author":"Costantini","year":"2017","journal-title":"Fish. Fish."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0003-2697(78)90342-1","article-title":"Determination of Malonaldehyde Precursor in Tissues by Thiobarbituric Acid Test","volume":"86","author":"Uchiyama","year":"1978","journal-title":"Anal. Biochem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e2404697","DOI":"10.1002\/advs.202404697","article-title":"Aspartate Metabolism-Driven Gut Microbiota Dynamics and RIP-Dependent Mitochondrial Function Counteract Oxidative Stress","volume":"12","author":"Jin","year":"2025","journal-title":"Adv. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"106655","DOI":"10.1016\/j.psj.2026.106655","article-title":"Dietary Supplemental L-Ornithine l-Aspartate Reduced Lipid Deposition and Alleviated Mitochondrial Oxidative Stress and Inflammatory Responses in the Liver of Broiler Chicken","volume":"105","author":"Zhang","year":"2026","journal-title":"Poult. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.aquaculture.2007.03.033","article-title":"Influence of Dietary Vitamin C and Bovine Lactoferrin on Blood Chemistry and Non-Specific Immune Responses of Japanese Eel, Anguilla japonica","volume":"267","author":"Ren","year":"2007","journal-title":"Aquaculture"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.fsi.2008.11.009","article-title":"Characterization of IgM of Indian Major Carps and Their Cross-Reactivity with Anti-Fish IgM Antibodies","volume":"26","author":"Bag","year":"2009","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_70","unstructured":"(2018). Guidance for the Care and Use of Laboratory Animals in China (Standard No. GB\/T 35892-2018)."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2190\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:16:54Z","timestamp":1784078214000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":70,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16142190"],"URL":"https:\/\/doi.org\/10.3390\/ani16142190","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]}}}