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Two revolutionary biotechnological systems, induced pluripotent stem cell (iPSC) reprogramming and CRISPR-Cas9-mediated genome editing, when combined, offer unparalleled accuracy and scalability for the biofabrication of avian flesh. In this review, we present a comprehensive pipeline that involves the ectopic expression of Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc) to reprogram primary somatic cells derived from Gallus gallus into induced pluripotent stem cells (iPSCs). This process is subsequently followed by targeted genome editing to enhance myogenic potential, growth efficiency, nutritional composition, and disease resistance. iPSCs are cultivated in a xeno-free bioreactor following genome editing, and subsequently directed to develop into myoblasts and mature myotubes. Three-dimensional tissue biofabrication is realized by combining biomaterial scaffolds and perfusion bioreactor systems, structuring an authentic muscle tissue matrix. These engineering platforms enable precise control over microenvironmental parameters, including oxygenation and nutrient perfusion. The resulting biofabricated poultry product is compositionally optimized, free of antibiotic residues, and exhibits a significantly reduced environmental footprint than poultry that is grown in the traditional way. This all-in-one solution solves important problems in food security, animal welfare, land use efficiency, and greenhouse gas emissions while also setting up a scalable biomanufacturing framework for making proteins for the next generation.<\/jats:p>","DOI":"10.3390\/ani16142193","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T07:29:39Z","timestamp":1784100579000},"page":"2193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biotechnological Strategies for Cultured Poultry Meat Biofabrication Through Induced Pluripotent Stem Cell Reprogramming and CRISPR-Cas9-Mediated Genome Editing"],"prefix":"10.3390","volume":"16","author":[{"given":"M Khuzema","family":"Niaz","sequence":"first","affiliation":[{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China, Yangzhou University, Yangzhou 225009, China"},{"name":"Key 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The Future of Food and Agriculture: Alternative Pathways to 2050, Food and Agriculture Organization of the United Nations."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0043933917000071","article-title":"Global Poultry Production: Current State and Future Outlook and Challenges","volume":"73","author":"Mottet","year":"2017","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_3","unstructured":"Alexandratos, N., and Bruinsma, J. (2012). World Agriculture Towards 2030\/2050: The 2012 Revision, FAO."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Naeem, M., Fatima, A., Raut, R., Kumar, R., Tushar, Z., Rahman, F., and Bourassa, D. (2026). Management and Genetic Approaches for Enhancing Meat Quality in Poultry Production Systems: A Comprehensive Review. Poultry, 5.","DOI":"10.3390\/poultry5010004"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Swayne, D.E., and Sims, L.D. (2021). Avian Influenza. Veterinary Vaccines: Principles and Applications, John Wiley & Sons, Ltd.","DOI":"10.1002\/9781119506287.ch18"},{"key":"ref_6","unstructured":"Steinfeld, H., Gerber, P., Wassenaar, T.D., Castel, V., and De Haan, C. (2006). Livestock\u2019s Long Shadow: Environmental Issues and Options, Food & Agriculture Org."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1038\/s43016-020-0112-z","article-title":"Scientific, Sustainability and Regulatory Challenges of Cultured Meat","volume":"1","author":"Post","year":"2020","journal-title":"Nat. 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Sci."},{"key":"ref_13","first-page":"511","article-title":"Small Molecules That Promote Self-Renewal of Stem Cells and Somatic Cell Reprogramming","volume":"16","author":"Chen","year":"2020","journal-title":"Reports"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ren, W., Wu, J., Lu, X., Zheng, D., Liu, G., Wu, G., Peng, Y., Jin, K., Li, G., and Han, W. (2024). Influence and Optimization of Diverse Culture Systems on Chicken Embryonic Stem Cell Culture. Genes, 15.","DOI":"10.3390\/genes15111400"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40104-018-0234-4","article-title":"Primordial Germ Cell-Mediated Transgenesis and Genome Editing in Birds","volume":"9","author":"Han","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_16","unstructured":"Kim, Y.M. (2018). Studies on Avian Germline Competent Stem Cells and Practical Application for Bioreactor System, Seoul National University."},{"key":"ref_17","first-page":"106814","article-title":"Adaptation of Induced Pluripotent Stem Cell Technology for Avian Species","volume":"237","author":"Katayama","year":"2025","journal-title":"Purification"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Schusser, B., and Doran, T. (2022). Advances in Genetic Engineering of the Avian Genome. Avian Immunology, Elsevier.","DOI":"10.1016\/B978-0-12-818708-1.00022-1"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zaib, G., Jin, K., Zuo, Q., Habib, M., Zhang, Y., and Li, B. (2023). Formation, Application, and Significance of Chicken Primordial Germ Cells: A Review. Animals, 13.","DOI":"10.3390\/ani13061096"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.biotechadv.2013.07.003","article-title":"Advances in Genetic Modification of Pluripotent Stem Cells","volume":"31","author":"Fontes","year":"2013","journal-title":"Biotechnol. Adv."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1177\/0023677221998400","article-title":"Genome Editing of Avian Species: Implications for Animal Use and Welfare","volume":"56","author":"Panda","year":"2022","journal-title":"Lab. Anim."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ballantyne, M., Doddamani, D., and McGrew, M.J. (2020). The Use of Genome Editing in Poultry Breeding. Advances in Poultry Genetics and Genomics, Burleigh Dodds Science Publishing.","DOI":"10.19103\/AS.2020.0065.29"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chen, X., Guo, Z., Tong, X., Wang, X., Liu, X., Nagai, H., Wu, P., Lu, J., Huss, D., and Tran, M. (2025). Derivation of Embryonic Stem Cells across Avian Species. Nat. Biotechnol.","DOI":"10.1038\/s41587-025-02833-3"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"He, J., Shi, N., Yao, H., Li, J., Wang, Y., and Zhang, J. (2025). Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26199426"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s40104-022-00768-2","article-title":"Poultry Genetic Heritage Cryopreservation and Reconstruction: Advancement and Future Challenges","volume":"13","author":"Sun","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.mod.2004.05.003","article-title":"Avian Pluripotent Stem Cells","volume":"121","author":"Petitte","year":"2004","journal-title":"Mech. Dev."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Choi, H.J., Han, Y., and Han, J.Y. (2025). A Step-by-Step Procedure for Producing Germline Chimera in Chicken via Primordial Germ Cell Transplantation. J. Vis. Exp., e69704.","DOI":"10.3791\/69704"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"50","DOI":"10.5483\/BMBRep.2023-0208","article-title":"Current Status and Future of Gene Engineering in Livestock","volume":"57","author":"Kwon","year":"2024","journal-title":"BMB Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/s41586-020-1978-5","article-title":"The Promise and Challenge of Therapeutic Genome Editing","volume":"578","author":"Doudna","year":"2020","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"RD24135","DOI":"10.1071\/RD24135","article-title":"The Role of Gene Editing, Organoids, and in Vitro Phenotyping in Sustainable Animal Production","volume":"37","author":"Redel","year":"2024","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/s41580-023-00697-6","article-title":"CRISPR Technologies for Genome, Epigenome and Transcriptome Editing","volume":"25","author":"Villiger","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"dmm049874","DOI":"10.1242\/dmm.049874","article-title":"New Advances in CRISPR\/Cas-Mediated Precise Gene-Editing Techniques","volume":"16","author":"Richardson","year":"2023","journal-title":"Dis. Model. Mech."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"23980","DOI":"10.1038\/srep23980","article-title":"Targeted Mutagenesis in Chicken Using CRISPR\/Cas9 System","volume":"6","author":"Oishi","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Kolluri, G., Naim, A., Kumar Kurva, S., Tyagi, J.S., Ansari, M.M., Tomar, S., Tiwari, A.K., and Chouhan, L. (2026). CRISPR\/Cas9-Based Programmable Genome Editing in Chickens: Concepts, Applications and Regulatory Issues. Front. Genome Ed., 7.","DOI":"10.3389\/fgeed.2025.1729535"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Khwatenge, C.N., and Nahashon, S.N. (2021). Recent Advances in the Application of CRISPR\/Cas9 Gene Editing System in Poultry Species. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.627714"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"dev157453","DOI":"10.1242\/dev.157453","article-title":"The Transcriptome of Early Chicken Embryos Reveals Signaling Pathways Governing Rapid Asymmetric Cellularization and Lineage Segregation","volume":"145","author":"Hwang","year":"2018","journal-title":"Development"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Xu, N., Wu, J., Ortiz-Vitali, J.L., Li, Y., and Darabi, R. (2021). Directed Differentiation of Human Pluripotent Stem Cells toward Skeletal Myogenic Progenitors and Their Purification Using Surface Markers. Cells, 10.","DOI":"10.3390\/cells10102746"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"vvaf220","DOI":"10.1093\/ijfood\/vvaf220","article-title":"Cultured Meat: Advances in Stem Cell Biology, Tissue Engineering, and Bioprocess Optimisation for Scalable and Sustainable Production\u2014A Review","volume":"60","author":"Kim","year":"2025","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kim, Y.M., Woo, S.J., and Han, J.Y. (2023). Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications. Genes, 14.","DOI":"10.3390\/genes14040899"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zahoor, N., Arif, A., Shuaib, M., Jin, K., Li, B., Li, Z., Pei, X., Zhu, X., Zuo, Q., and Niu, Y. (2024). Induced Pluripotent Stem Cells in Birds: Opportunities and Challenges for Science and Agriculture. Vet. Sci., 11.","DOI":"10.3390\/vetsci11120666"},{"key":"ref_41","first-page":"483","article-title":"Advancement of Animal and Poultry Nutrition: Harnessing the Power of CRISPR-Cas Genome Editing Technology","volume":"11","author":"Mishu","year":"2024","journal-title":"Research"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Pratumkaew, P., Issaragrisil, S., and Luanpitpong, S. (2021). Induced Pluripotent Stem Cells as a Tool for Modeling Hematologic Disorders and as a Potential Source for Cell-Based Therapies. Cells, 10.","DOI":"10.3390\/cells10113250"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/s44222-023-00136-3","article-title":"Genome-Edited Chickens","volume":"1","author":"Bakhshandeh","year":"2023","journal-title":"Nat. Rev. Bioeng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s40104-026-01358-2","article-title":"Advancing Immunity and Disease Resistance in Chickens through Genome Editing","volume":"17","author":"Sid","year":"2026","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"117567","DOI":"10.1016\/j.theriogenology.2025.117567","article-title":"Generation of Chicken Induced Pluripotent Stem Cells (IPSCs) from Somatic Cells Using Multi-Lentiviral Transduction and OAC2","volume":"247","author":"Liu","year":"2025","journal-title":"Theriogenology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/nature04831","article-title":"Germline Transmission of Genetically Modified Primordial Germ Cells","volume":"441","author":"Diamond","year":"2006","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e70915","DOI":"10.1111\/1750-3841.70915","article-title":"Cultured Meat: A Multidimensional Review of Technological, Nutritional, Ethical, and Regulatory Advances (2020\u20132025)","volume":"91","author":"Nogueira","year":"2026","journal-title":"J. Food Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e70221","DOI":"10.1111\/1541-4337.70221","article-title":"Scaling Cultured Meat: Challenges and Solutions for Affordable Mass Production","volume":"24","author":"Gu","year":"2025","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Dupont, J., Harms, T., and Fiebelkorn, F. (2022). Acceptance of Cultured Meat in Germany\u2014Application of an Extended Theory of Planned Behaviour. Foods, 11.","DOI":"10.3390\/foods11030424"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Park, J.S., Lee, K.Y., and Han, J.Y. (2020). Precise Genome Editing in Poultry and Its Application to Industries. Genes, 11.","DOI":"10.3390\/genes11101182"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.meatsci.2012.04.005","article-title":"Predicting the Likelihood of Developing Boar Taint: Early Physical Indicators in Entire Male Pigs","volume":"92","author":"Bekaert","year":"2012","journal-title":"Meat Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2193\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:20:13Z","timestamp":1784262013000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/14\/2193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":51,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ani16142193"],"URL":"https:\/\/doi.org\/10.3390\/ani16142193","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]}}}