{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T04:51:10Z","timestamp":1785905470977,"version":"3.56.0"},"reference-count":56,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32401366"],"award-info":[{"award-number":["32401366"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32401366"],"award-info":[{"award-number":["32401366"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"crossref","award":["252300420712"],"award-info":[{"award-number":["252300420712"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"crossref","award":["242102320253"],"award-info":[{"award-number":["242102320253"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"crossref","award":["262102321107"],"award-info":[{"award-number":["262102321107"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Fund for High-Level Talents of Pingdingshan University","award":["PXY-BSQD-2023041"],"award-info":[{"award-number":["PXY-BSQD-2023041"]}]},{"award":["PXY-BSQD-2023041"],"award-info":[{"award-number":["PXY-BSQD-2023041"]}],"id":[{"id":"https:\/\/ror.org\/026c29h90","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Henan Key Laboratory for Germplasm Innovation and Utilization of Eco-economic Woody Plant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Insect herbivores often exhibit host preference and feeding differentiation underpinning fine-scale niche partitioning, yet their linkage to larval performance remains poorly understood. On the Qinghai\u2013Tibet Plateau, two sympatric leaf beetles, Chrysomela vigintipunctata alticola (CV) and Phratora vitellinae (PV) (both Coleoptera: Chrysomelidae), co-occur on two willow species occupying distinct microhabitats: CV predominantly uses abundant Salix atopantha (SW), while PV specializes on rare Salix wangiana var. tibetica (LW) (both Malpighiales: Salicaceae). To disentangle intrinsic feeding response from natal-host induction, we combined dual-choice feeding bioassays with controlled natal-host rearing experiments using first-instar larvae originating from both hosts, and evaluated fitness consequences in terms of morphology and instar duration. PV larvae exhibited a consistent preference for LW, coupled with wider head capsules. In contrast, CV larvae showed no intrinsic preference; specifically, those from SW remained non-selective, while those from LW developed a delayed preference. CV larvae developed faster on nitrogen-rich SW, whereas LW contained higher salicylate esters. These results indicate that PV prioritizes growth and chemical defensive sequestration on LW, whereas CV prioritizes rapid development by utilizing the nutrient-rich SW. Our results reveal distinct resource-use patterns and life-history strategies that facilitate local co-occurrence of the two leaf beetles within the alpine meadow ecosystem.<\/jats:p>","DOI":"10.3390\/ani16152395","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:57:56Z","timestamp":1785761876000},"page":"2395","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feeding Preference Differentiation in Sympatric Willow Leaf Beetles Driven by Host Leaf Trait Divergence"],"prefix":"10.3390","volume":"16","author":[{"given":"Lixuan","family":"Kou","sequence":"first","affiliation":[{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China"},{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China"},{"name":"College of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghang","family":"Lian","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China"},{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peisong","family":"Liu","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Germplasm Innovation and Utilization of Eco-Economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China"},{"name":"College of Geography and Environmental Engineering, Pingdingshan University, Pingdingshan 467000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/BF00378451","article-title":"Phenolic glycosides govern the food selection pattern of willow feeding leaf beetles","volume":"67","author":"Tahvanainen","year":"1985","journal-title":"Oecologia"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Schoonhoven, L.M., Van Loon, J.J., and Dicke, M. 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