{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T09:30:30Z","timestamp":1786440630708,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2026A1515011962"],"award-info":[{"award-number":["2026A1515011962"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2024A1515030165"],"award-info":[{"award-number":["2024A1515030165"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["326MS0347"],"award-info":[{"award-number":["326MS0347"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2023TD37"],"award-info":[{"award-number":["2023TD37"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2026SJHX1"],"award-info":[{"award-number":["2026SJHX1"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2026XK02"],"award-info":[{"award-number":["2026XK02"]}]},{"name":"Guangdong Special Support Program","award":["2024TQ08A617"],"award-info":[{"award-number":["2024TQ08A617"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32373127"],"award-info":[{"award-number":["32373127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32373127"],"award-info":[{"award-number":["32373127"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100022960","name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","doi-asserted-by":"crossref","award":["2024CXTD26"],"award-info":[{"award-number":["2024CXTD26"]}],"id":[{"id":"10.13039\/100022960","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Program of Guangzhou","award":["2025A04J5171"],"award-info":[{"award-number":["2025A04J5171"]}]},{"name":"Guangdong Province Rural Revitalization Strategy Special Fund","award":["2023-SJS-00-001"],"award-info":[{"award-number":["2023-SJS-00-001"]}]},{"name":"China\u2013ASEAN Maritime Cooperation Fund","award":["CAMC-2018F"],"award-info":[{"award-number":["CAMC-2018F"]}]},{"name":"Guangdong Province Science and Technology Commissioner Program","award":["KTP20240444"],"award-info":[{"award-number":["KTP20240444"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Body weight is the principal economic trait in snakehead aquaculture, yet the genetic architecture of growth in the northern snakehead (Channa argus) remains largely unexplored. We performed a genome-wide association study (GWAS) for body weight in 205 cultured C. argus genotyped with a 50K SNP array. After quality control, 47,123 autosomal SNPs were tested under a univariate linear mixed model in GEMMA that accounted for genomic relatedness. One SNP on chromosome 7 (Chr07:1,329,732) exceeded the Bonferroni genome-wide threshold, and 14 SNPs reached a suggestive threshold, forming two candidate clusters on Chr07 and Chr24. Annotating candidate intervals (\u00b1100 kb) against the C. argus genome assembly yielded 93 candidate genes. Hypergeometric enrichment identified 44 Gene Ontology (GO) terms and 4 KEGG pathways, dominated by endolysosomal lumen, serine-type endopeptidase activity, collagen\/extracellular-matrix catabolism, bone resorption, and the apoptosis, lysosome, phagocytosis and antigen-processing pathways. The single genome-wide significant SNP fell in an intron of a collagen alpha-1(II) chain gene (COL2A1\/zgc:113232), consistent with the collagen\/extracellular-matrix enrichment signal; other plausible growth-related candidates included CTSS, S100A4, PYGM, DLAT, MSX2 and ZNRF3. The functional signal was largely driven by a tandem protease\/cathepsin gene cluster on Chr07, indicating a regional rather than a genome-wide pathway effect. The lead SNP genotype was associated with a ~240 g (\u224830%) difference in body weight, and this effect was reproduced in an independent cohort of 200 fish. These findings provide the first GWAS-based candidate loci for body weight in C. argus and a promising marker for future marker-assisted breeding, though validation to date is limited to a single additional cohort from the same broodstock source; broader multi-population testing will be needed before routine application.<\/jats:p>","DOI":"10.3390\/ani16162471","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T01:20:03Z","timestamp":1786324803000},"page":"2471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Genome-Wide Association Study Identifies Candidate SNPs and Genes Associated with Body Weight in the Northern Snakehead (Channa argus) Using a 50K SNP Array"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8301-4595","authenticated-orcid":false,"given":"Haiyang","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huai\u2019an 223300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3537-4446","authenticated-orcid":false,"given":"Mi","family":"Ou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1574-936X","authenticated-orcid":false,"given":"Qing","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhan","family":"Fei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xincheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Qingyuan Zhuoyue Aquatic Co., Ltd., Qingyuan 511500, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunci","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1111\/raq.12750","article-title":"Genome-wide association and genomic selection in aquaculture","volume":"15","author":"Moen","year":"2023","journal-title":"Rev. Aquac."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/raq.12098","article-title":"A review of genotype-by-environment interaction and micro-environmental sensitivity in aquaculture species","volume":"8","author":"Gjerde","year":"2016","journal-title":"Rev. Aquac."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s00438-017-1406-1","article-title":"Identification of novel genes significantly affecting growth in catfish through GWAS analysis","volume":"293","author":"Li","year":"2018","journal-title":"Mol. Genet. Genom."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"738513","DOI":"10.1016\/j.aquaculture.2022.738513","article-title":"Genome-wide association study reveals growth-related markers and candidate genes for selection in Chinese longsnout catfish (Leiocassis longirostris)","volume":"560","author":"Mou","year":"2022","journal-title":"Aquaculture"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1534\/g3.119.400116","article-title":"Genome-wide association study and cost-efficient genomic predictions for growth and fillet yield in Nile tilapia (Oreochromis niloticus)","volume":"9","author":"Yoshida","year":"2019","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1101\/gr.278476.123","article-title":"The Chinese longsnout catfish genome provides novel insights into the feeding preference and corresponding metabolic strategy of carnivores","volume":"34","author":"Liu","year":"2024","journal-title":"Genome Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.1534\/g3.120.401343","article-title":"Development and validation of an open-access SNP array for Nile tilapia (Oreochromis niloticus)","volume":"10","author":"Robledo","year":"2020","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Jiang, B., Tao, Y., Tao, W., Lu, S., Badran, M.F., Saleh, M.H.L., Aboueleila, R.H.M., Xu, P., Qiang, J., and Liu, K. (2025). Genome-wide association study and RNA-Seq analysis uncover candidate genes controlling growth traits in red tilapia (Oreochromis spp.) under hyperosmotic stress. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26136492"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Barr\u00eda, A., Benzie, J.A.H., Houston, R.D., De Koning, D.J., and de Verdal, H. (2021). Genomic selection and genome-wide association study for feed-efficiency traits in a farmed Nile tilapia (Oreochromis niloticus) population. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.737906"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"739979","DOI":"10.1016\/j.aquaculture.2023.739979","article-title":"Genome-wide association study reveals growth-related SNPs and candidate genes in grass carp (Ctenopharyngodon idella)","volume":"577","author":"Hao","year":"2023","journal-title":"Aquaculture"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"741129","DOI":"10.1016\/j.aquaculture.2024.741129","article-title":"Identification of novel SNPs and candidate genes significantly affecting growth in grass carp (Ctenopharyngodon idella) through GWAS analysis","volume":"591","author":"Zhang","year":"2024","journal-title":"Aquaculture"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Chen, Y., Yu, Q., Lv, W., Sheng, T., Gui, L., Qiu, J., Xu, X., and Li, J. (2025). Genome-wide association analysis and genomic selection for growth traits in grass carp (Ctenopharyngodon idella). Animals, 15.","DOI":"10.3390\/ani15131888"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7259","DOI":"10.1038\/s41598-020-64037-w","article-title":"Genome-wide analysis for growth at two growth stages in a new fast-growing common carp strain (Cyprinus carpio L.)","volume":"10","author":"Su","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sun, Z., Fu, Y., Zhu, X., Zhang, R., Shu, Y., Zheng, X., and Hu, G. (2025). A framework integrating GWAS and genomic selection to enhance prediction accuracy of economic traits in common carp (Cyprinus carpio). Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26147009"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1007\/s10126-022-10176-2","article-title":"Genome-wide association study reveals SNPs and candidate genes related to growth and body shape in bighead carp (Hypophthalmichthys nobilis)","volume":"24","author":"Chen","year":"2022","journal-title":"Mar. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tsai, H.Y., Hamilton, A., Tinch, A.E., Guy, D.R., Gharbi, K., Stear, M.J., Matika, O., Bishop, S.C., and Houston, R.D. (2015). Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array. BMC Genom., 16.","DOI":"10.1186\/s12864-015-2117-9"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Tsai, H., Hamilton, A., Guy, D., Tinch, A., Bishop, S., and Houston, R. (2016). Verification of SNPs associated with growth traits in two populations of farmed Atlantic salmon. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17010005"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"jkad138","DOI":"10.1093\/g3journal\/jkad138","article-title":"Generation of the first chromosome-level de novo genome assembly and the development and validation of a 50K SNP array for the St. John River aquaculture strain of North American Atlantic salmon (Salmo salar)","volume":"13","author":"Gao","year":"2023","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ali, A., Al-Tobasei, R., Lourenco, D., Leeds, T., Kenney, B., and Salem, M. (2020). Genome-wide identification of loci associated with growth in rainbow trout. BMC Genom., 21.","DOI":"10.1186\/s12864-020-6617-x"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Salem, M., Al-Tobasei, R., Ali, A., Lourenco, D., Gao, G., Palti, Y., Kenney, B., and Leeds, T.D. (2018). Genome-wide association analysis with a 50K transcribed gene SNP-chip identifies QTL affecting muscle yield in rainbow trout. Front. Genet., 9.","DOI":"10.3389\/fgene.2018.00387"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Lyu, D., Yu, Y., Wang, Q., Luo, Z., Zhang, Q., Zhang, X., Xiang, J., and Li, F. (2021). Identification of growth-associated genes by genome-wide association study and their potential application in the breeding of Pacific white shrimp (Litopenaeus vannamei). Front. Genet., 12.","DOI":"10.3389\/fgene.2021.611570"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mar\u00edn-Nahuelpi, R., Urz\u00faa-Encina, C., Xuereb, A., Mallet, M., Mallet, A., Normandeau, E., Babin, C., Y\u00e1\u00f1ez, J.M., and Bernatchez, L. (2025). Genome-wide association study and genomic selection for growth-related traits in Eastern oyster (Crassostrea virginica). BMC Genom., 26.","DOI":"10.1186\/s12864-025-12100-z"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"734216","DOI":"10.1016\/j.aquaculture.2019.734216","article-title":"Genome-wide association study reveals E2F3 as the candidate gene for scallop growth","volume":"511","author":"Ning","year":"2019","journal-title":"Aquaculture"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"742225","DOI":"10.1016\/j.aquaculture.2025.742225","article-title":"A genome-wide association study of growth traits in \u201cGreen Pacific hybrid abalone\u201d (Haliotis discus hannai \u2640 \u00d7 H. fulgens \u2642)","volume":"601","author":"Yao","year":"2025","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhou, T., Chen, B., Ke, Q., Zhao, J., Pu, F., Wu, Y., Chen, L., Zhou, Z., Bai, Y., and Pan, Y. (2020). Development and evaluation of a high-throughput single-nucleotide polymorphism array for large yellow croaker (Larimichthys crocea). Front. Genet., 11.","DOI":"10.3389\/fgene.2020.571751"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"739021","DOI":"10.1016\/j.aquaculture.2022.739021","article-title":"Development and evaluation of a liquid SNP array for large yellow croaker (Larimichthys crocea)","volume":"563","author":"Wang","year":"2023","journal-title":"Aquaculture"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"737879","DOI":"10.1016\/j.aquaculture.2021.737879","article-title":"Genome-wide association study reveals growth-related SNPs and candidate genes in mandarin fish (Siniperca chuatsi)","volume":"550","author":"Zhou","year":"2022","journal-title":"Aquaculture"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"740778","DOI":"10.1016\/j.aquaculture.2024.740778","article-title":"Identification of SNPs and candidate genes associated with growth performance in all-female mandarin fish (Siniperca chuatsi) by a genome-wide association study","volume":"586","author":"Liu","year":"2024","journal-title":"Aquaculture"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"740777","DOI":"10.1016\/j.aquaculture.2024.740777","article-title":"Identification of SNPs and candidate genes associated with growth using GWAS and transcriptome analysis in Coilia nasus","volume":"586","author":"Yu","year":"2024","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"739194","DOI":"10.1016\/j.aquaculture.2022.739194","article-title":"First genome-wide association study and genomic prediction for growth traits in spotted sea bass (Lateolabrax maculatus) using whole-genome resequencing","volume":"566","author":"Zhang","year":"2023","journal-title":"Aquaculture"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Oikonomou, S., Samaras, A., Tekeoglou, M., Loukovitis, D., Dimitroglou, A., Kottaras, L., Papanna, K., Papaharisis, L., Tsigenopoulos, C.S., and Pavlidis, M. (2022). Genomic selection and genome-wide association analysis for stress response, disease resistance and body weight in European seabass (Dicentrarchus labrax). Animals, 12.","DOI":"10.3390\/ani12030277"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.eng.2020.06.017","article-title":"Development of a 50K SNP array for Japanese flounder and its application in genomic selection for disease resistance","volume":"7","author":"Zhou","year":"2021","journal-title":"Engineering"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"742745","DOI":"10.1016\/j.aquaculture.2025.742745","article-title":"Identification of KASP-SNP markers correlated with both growth and hypoxia tolerance traits in blunt snout bream (Megalobrama amblycephala)","volume":"608","author":"Zhuo","year":"2025","journal-title":"Aquaculture"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"10510","DOI":"10.1007\/s10126-025-10510-4","article-title":"Identification of growth-related SNPs and candidate genes in the genome of zig-zag eel (Mastacembelus armatus) by GWAS","volume":"27","author":"Han","year":"2025","journal-title":"Mar. Biotechnol."},{"key":"ref_35","first-page":"101932","article-title":"A genome-wide association study to identify growth-related SNPs and genes in blotched snakehead (Channa maculata)","volume":"35","author":"Liu","year":"2024","journal-title":"Aquacult. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"736541","DOI":"10.1016\/j.aquaculture.2021.736541","article-title":"Construction of a high-density linkage map and QTL detection of growth and sex in blotched snakehead (Channa maculata)","volume":"538","author":"Liu","year":"2021","journal-title":"Aquaculture"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1038\/s41597-024-04314-9","article-title":"Chromosome-level genome assembly of the northern snakehead (Channa argus) using PacBio and Hi-C technologies","volume":"11","author":"Sun","year":"2024","journal-title":"Sci. Data"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"742020","DOI":"10.1016\/j.aquaculture.2024.742020","article-title":"Development and evaluation of a 50K SNP array for blotched snakehead (Channa maculata)","volume":"598","author":"Cui","year":"2025","journal-title":"Aquaculture"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"743737","DOI":"10.1016\/j.aquaculture.2026.743737","article-title":"Development of a 50K liquid SNP array and its application in GWAS and population genomics of northern snakehead (Channa argus)","volume":"616","author":"Zhang","year":"2026","journal-title":"Aquaculture"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/ng1702","article-title":"A unified mixed-model method for association mapping that accounts for multiple levels of relatedness","volume":"38","author":"Yu","year":"2006","journal-title":"Nat. Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/ng.2310","article-title":"Genome-wide efficient mixed-model analysis for association studies","volume":"44","author":"Zhou","year":"2012","journal-title":"Nat. Genet."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"giab070","DOI":"10.1093\/gigascience\/giab070","article-title":"Chromosome-level genome assemblies of Channa argus and Channa maculata and comparative analysis of their temperature adaptability","volume":"10","author":"Ou","year":"2021","journal-title":"GigaScience"},{"key":"ref_43","first-page":"100768","article-title":"Performance evaluation of XY all-male hybrids derived from XX female Channa argus and YY super-males Channa maculata","volume":"20","author":"Ou","year":"2021","journal-title":"Aquacult. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3109\/19401736.2012.752469","article-title":"The complete mitochondrial genome of Channa argus, Channa maculata and hybrid snakehead fish [Channa maculata (\u2640) \u00d7 Channa argus (\u2642)]","volume":"24","author":"Zhu","year":"2013","journal-title":"Mitochondrial DNA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"gix011","DOI":"10.1093\/gigascience\/gix011","article-title":"Draft genome of the northern snakehead, Channa argus","volume":"6","author":"Xu","year":"2017","journal-title":"GigaScience"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Zhou, C., Li, J., Wu, Y., Zou, Y., Yuan, D., Deng, X., Lei, L., Su, J., Zhu, C., and Ye, H. (2022). Chromosome-scale assembly and characterization of the albino northern snakehead, Channa argus var. (Teleostei: Channidae) genome. Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.839225"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Liu, H., Zhang, J., Cui, T., Xia, W., Luo, Q., Fei, S., Zhu, X., Chen, K., Zhao, J., and Ou, M. (2024). Genome-wide association studies (GWAS) and transcriptome analysis reveal male heterogametic sex-determining regions and candidate genes in northern snakeheads (Channa argus). Int. J. Mol. Sci., 25.","DOI":"10.2139\/ssrn.4939023"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"98733","DOI":"10.18632\/oncotarget.21924","article-title":"An NGS-based approach for the identification of sex-specific markers in snakehead (Channa argus)","volume":"8","author":"Ou","year":"2017","journal-title":"Oncotarget"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1007\/s10126-019-09884-z","article-title":"Constructing high-density genetic maps and developing sex-linked markers in northern snakehead (Channa argus)","volume":"21","author":"Wang","year":"2019","journal-title":"Mar. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"735499","DOI":"10.1016\/j.aquaculture.2020.735499","article-title":"A rapid method of sex-specific marker discovery based on NGS and determination of the XX\/XY sex-determination system in Channa maculata","volume":"528","author":"Yang","year":"2020","journal-title":"Aquaculture"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"741449","DOI":"10.1016\/j.aquaculture.2024.741449","article-title":"Sex-linked DNA marker screening and characterization in albino northern snakehead (Channa argus var.) via third-generation sequencing and pool resequencing","volume":"594","author":"Dai","year":"2025","journal-title":"Aquaculture"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"734760","DOI":"10.1016\/j.aquaculture.2019.734760","article-title":"High-density genetic linkage map and QTL fine mapping of growth and sex in snakehead (Channa argus)","volume":"519","author":"Liu","year":"2020","journal-title":"Aquaculture"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"739771","DOI":"10.1016\/j.aquaculture.2023.739771","article-title":"Comparative study of candidate sex determination regions in snakeheads (Channa argus and Channa maculata) and development of novel sex markers","volume":"575","author":"Sun","year":"2023","journal-title":"Aquaculture"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"220235","DOI":"10.1098\/rsob.220235","article-title":"The genetic basis and potential molecular mechanism of yellow-albino northern snakehead (Channa argus)","volume":"13","author":"Sun","year":"2023","journal-title":"Open Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"s13742-015-0047-8","DOI":"10.1186\/s13742-015-0047-8","article-title":"Second-generation PLINK: Rising to the challenge of larger and richer datasets","volume":"4","author":"Chang","year":"2015","journal-title":"GigaScience"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.gpb.2020.10.007","article-title":"rMVP: A memory-efficient, visualization-enhanced, and parallel-accelerated tool for genome-wide association study","volume":"19","author":"Yin","year":"2021","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: A flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-mapper v2: Functional annotation, orthology assignments, and domain prediction at the metagenomic scale","volume":"38","author":"Cantalapiedra","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41592-021-01101-x","article-title":"Sensitive protein alignments at tree-of-life scale using DIAMOND","volume":"18","author":"Buchfink","year":"2021","journal-title":"Nat. Methods"},{"key":"ref_60","first-page":"D309","article-title":"eggNOG 5.0: A hierarchical, functionally and phylogenetically annotated orthology resource","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.ydbio.2011.06.020","article-title":"Identification of an evolutionarily conserved regulatory element of the zebrafish col2a1a gene","volume":"357","author":"Dale","year":"2011","journal-title":"Dev. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.gep.2015.07.004","article-title":"Collagen duplicate genes of bone and cartilage participate during regeneration of zebrafish fin skeleton","volume":"19","author":"Duran","year":"2015","journal-title":"Gene Expr. Patterns"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Amoroso, G., Ventura, T., Cobcroft, J.M., Adams, M.B., Elizur, A., and Carter, C.G. (2016). Multigenic delineation of lower jaw deformity in triploid Atlantic salmon (Salmo salar L.). PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0168454"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"111157","DOI":"10.1016\/j.ygeno.2025.111157","article-title":"Construction of a genome-wide linkage map and QTL mapping for growth and hypoxia tolerance traits in Chinese longsnout catfish (Leiocassis longirostris)","volume":"117","author":"Liang","year":"2025","journal-title":"Genomics"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2471\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:37:04Z","timestamp":1786437424000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,8]]},"references-count":67,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ani16162471"],"URL":"https:\/\/doi.org\/10.3390\/ani16162471","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,8]]}}}