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The release of EVs is increased in mammary tumors compared to normal tissue, with the quantity generally correlating with the tumor\u2019s histological grade and aggressiveness. EVs carry a varied molecular load, mainly composed of proteins, RNAs, including microRNAs (miRNAs), lipids, and nucleic acids. These contents reflect the cell of origin and are related to the tumor profile (adenoma, adenocarcinoma, or carcinoma). EVs influence biological processes such as cell proliferation, migration, and invasion, as well as interfere with the immune response, potentially favoring tumor growth, disease progression, and metastasis formation, since they act as intercellular messengers, modulating the tumor microenvironment. Despite substantial advances in research related to EVs in human breast cancer, there is still a significant lack of studies focused on veterinary oncology, especially regarding the association of EVs with CMTs. In this context, this review aimed to compile the evidence available to highlight the importance of EVs in CMTs in female dogs, especially as promising tools for innovative strategies in veterinary medicine.<\/jats:p>","DOI":"10.3390\/ani16162478","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T08:08:53Z","timestamp":1786349333000},"page":"2478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extracellular Vesicles as Emerging Tools in Canine Mammary Tumors: Potential Applications and Perspectives"],"prefix":"10.3390","volume":"16","author":[{"given":"Luadna","family":"dos Santos e Silva","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Cancer Research (LIMC), Faculty of Medicine of S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0146-9041","authenticated-orcid":false,"given":"Debora Aparecida","family":"Pires de Campos Zuccari","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cancer Research (LIMC), Faculty of Medicine of S\u00e3o Jos\u00e9 do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6584-6566","authenticated-orcid":false,"given":"Guilherme Henrique","family":"Tamarindo","sequence":"additional","affiliation":[{"name":"Brazilian National Biosciences Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Rua Giuseppe M\u00e1ximo Scolfaro, Campinas 13083-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1188-7497","authenticated-orcid":false,"given":"Cintia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu 18618-689, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5916-8738","authenticated-orcid":false,"given":"Caroline","family":"Anacleto Rinaldi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu 18618-689, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-3396","authenticated-orcid":false,"given":"Luiz Gustavo","family":"de Almeida Chuffa","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu 18618-689, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Petrou\u0161kov\u00e1, P., Hud\u00e1kov\u00e1, N., Malovesk\u00e1, M., Humen\u00edk, F., and Cizkova, D. 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