{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T08:37:32Z","timestamp":1786523852335,"version":"build-2736575974"},"reference-count":81,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation Fund","doi-asserted-by":"publisher","award":["32260811"],"award-info":[{"award-number":["32260811"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Inner Mongolia Natural Science Foundation Fund","award":["2024LHMS03051"],"award-info":[{"award-number":["2024LHMS03051"]}]},{"name":"Inner Mongolia Autonomous Region key research and achievement transformation project","award":["2023YFDZ0023"],"award-info":[{"award-number":["2023YFDZ0023"]}]},{"name":"Inner Mongolia Autonomous Region key research and achievement transformation project","award":["2024KJHZ0019"],"award-info":[{"award-number":["2024KJHZ0019"]}]},{"name":"Inner Mongolia Autonomous Region key research and achievement transformation project","award":["2025KJHZ0066"],"award-info":[{"award-number":["2025KJHZ0066"]}]},{"name":"Inner Mongolia Autonomous Region Youth Science and Technology Talents Project","award":["NJYT23014"],"award-info":[{"award-number":["NJYT23014"]}]},{"name":"Inner Mongolia Autonomous Region Basic Research Funds for Directly Affiliated Universities","award":["BR250103"],"award-info":[{"award-number":["BR250103"]}]},{"name":"Inner Mongolia Education Department Special Research Project For First Class Disciplines","award":["YLXKZX-NND-007"],"award-info":[{"award-number":["YLXKZX-NND-007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Cold exposure severely impairs intestinal homeostasis and growth in young lambs. This study investigated the protective effects of Artemisia argyi polysaccharides (AAPs) on intestinal health in cold-exposed suckling lambs. Twenty-four 7-day-old lambs were randomly allocated to receive a milk replacer supplemented with 0 (Control, C), 0.5 (Low-dose, LA), 1.0 (Medium-dose, MA), or 2.0 g\/kg (High-dose, HA) of AAPs for 14 days under cold conditions (\u22128 \u00b0C to \u22125 \u00b0C). The results showed that the MA group significantly increased ADG and decreased the F\/G (p &lt; 0.05) compared to the control. Both LA and MA groups significantly improved EE and Ca digestibility, while the MA group further increased CP and P digestibility (p &lt; 0.05). The LA group enhanced jejunal chymotrypsin and lipase activities, whereas the MA group elevated chymotrypsin, lipase, and \u03b1-amylase activities (p &lt; 0.05). Additionally, both LA and MA upregulated Nrf2 mRNA expression (and downregulated Keap1 mRNA expression in the jejunum), suggesting activation of the Nrf2\/Keap1 pathway; the MA group significantly upregulated CAT, GSH-Px, and SOD1 mRNA expressions, enhanced SOD and CAT activities, and reduced colonic MDA content (p &lt; 0.05). The MA group also significantly elevated the VH\/CD across the small intestine, upregulated tight junction genes (Claudin-1 and Claudin-4 in jejunum; ZO-1 and Claudin-1 in colon), and decreased serum D-LA and DAO levels (p &lt; 0.05). Cecal microbiota sequencing showed that AAPs enriched the relative abundance of Anaerofilum and reduced Akkermansia. In conclusion, 1.0 g\/kg AAP supplementation effectively mitigates intestinal oxidative stress, improves barrier integrity, and modulates gut microbiota, thereby enhancing nutrient digestibility and growth performance in cold-stressed suckling lambs.<\/jats:p>","DOI":"10.3390\/ani16162512","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:57:26Z","timestamp":1786521446000},"page":"2512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Artemisia argyi Polysaccharides Protect Intestinal Health by Enhancing Antioxidant Capacity in Suckling Lambs Under Cold Exposure"],"prefix":"10.3390","volume":"16","author":[{"given":"Shuang","family":"Zheng","sequence":"first","affiliation":[{"name":"College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-3566","authenticated-orcid":false,"given":"Xiao","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Song","sequence":"additional","affiliation":[{"name":"College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0604-1607","authenticated-orcid":false,"given":"Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4600-6291","authenticated-orcid":false,"given":"Dengsheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Animal-Centered Environments Group, Department of Biosystems and Engineering, Swedish University of Agricultural Sciences (SLU), P.O. Box 190, 234 22 Lomma, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Wang, G., Zhang, Y., Zhao, S., and Han, C. (2021). Climate Change Is Likely to Alter Sheep and Goat Distributions in Mainland China. Front. Environ. Sci., 9.","DOI":"10.3389\/fenvs.2021.748734"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2599631","DOI":"10.1080\/10495398.2025.2599631","article-title":"Genetic Diversity Assessment of Guangling Large-Tail Sheep and Introduced Breeds in Shanxi Province Using Microsatellite Markers and Inference of Heterosis Potential","volume":"36","author":"Xue","year":"2025","journal-title":"Anim. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Herath, H.M.G.P., Pain, S.J., Kenyon, P.R., Blair, H.T., and Morel, P.C.H. (2021). 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