{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:50:15Z","timestamp":1793253015328,"version":"4.5.0"},"reference-count":55,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BioDose ul., Pozna\u0144, Poland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The aim of this study was to evaluate the effects of a Bacillus subtilis-derived postbiotic on apparent total tract nutrient digestibility, faecal fermentation characteristics, gut microbiota, immune response, blood biochemistry, hematological parameters, serum trace minerals, and coat quality in healthy senior dogs. Twenty-one healthy senior Golden Retriever dogs (9 \u00b1 1.0 years old) were randomly allocated to three dietary treatments for 28 days: a control diet (CON) or the same diet supplemented with 0.3 (POS1) or 0.6 mL\/day (POS2) of a Bacillus subtilis-derived postbiotic. Apparent nutrient digestibility, faecal characteristics, blood variables, and coat quality were evaluated, whereas faecal bacterial communities were characterized by 16S rRNA gene amplicon sequencing, followed by taxonomic classification and alpha- and beta-diversity analyses. Postbiotic supplementation significantly improved crude protein and crude fiber digestibility while reducing faecal pH and ammonia concentrations and increasing acetate, propionate, and total short-chain fatty acid concentrations (p &lt; 0.05). Alpha diversity was minimally affected, with a significant difference observed only for amplicon sequence variant (ASV) richness, whereas beta diversity analyses demonstrated significant alterations in overall microbial community composition. Postbiotic supplementation increased the relative abundance of Faecalibacterium, Ligilactobacillus, Bifidobacterium, Prevotella, Turicibacter, and Fusobacterium, while reducing Bacteroides and Achromobacter. Serum IgG concentrations increased, whereas cholesterol and triglyceride concentrations decreased in supplemented dogs (p &lt; 0.05). Coat morphology and gloss also improved, without adverse effects on hematological, biochemical, or serum trace mineral parameters. In conclusion, Bacillus subtilis-derived postbiotic supplementation improved nutrient utilization, beneficially modulated faecal microbial ecology and fermentation, enhanced selected metabolic and immune responses, and improved coat quality, supporting its potential as a functional dietary strategy for promoting gastrointestinal and systemic health in healthy senior dogs.<\/jats:p>","DOI":"10.3390\/ani16162515","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:02:11Z","timestamp":1786532531000},"page":"2515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Complementary Feed with Postbiotics Derived from Bacillus subtilis on Nutrient Digestibility, Faecal Characteristics, Gut Microbiota, Metabolic and Immune Responses, and Coat Quality in Healthy Senior Dogs"],"prefix":"10.3390","volume":"16","author":[{"given":"Oguzhan","family":"Kahraman","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42250, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martyna","family":"Wilk","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Management, Wroc\u0142aw University of Environmental and Life Sciences, 25 C.K. Norwida Street, 50-375 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Bochynek","sequence":"additional","affiliation":[{"name":"BioDose, ul. Warpnowska 28, 60-453 Pozna\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8692-050X","authenticated-orcid":false,"given":"Krzysztof","family":"Bojanowski","sequence":"additional","affiliation":[{"name":"Sunny BioDiscovery, Inc., 972 E. Main Street, Santa Paula, CA 93060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7538-3435","authenticated-orcid":false,"given":"Geovani","family":"Quijas","sequence":"additional","affiliation":[{"name":"Sunny BioDiscovery, Inc., 972 E. Main Street, Santa Paula, CA 93060, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0832-9209","authenticated-orcid":false,"given":"Zekeriya Safa","family":"Inanc","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42250, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Inal","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Sel\u00e7uk University, Konya 42250, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-1436","authenticated-orcid":false,"given":"Ibrar","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Xiamen Creciente Agritech Co., Ltd., No. 482, Xiamen 361022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9821-8793","authenticated-orcid":false,"given":"Agnieszka","family":"Lewi\u0144ska","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Wroc\u0142aw, Joliot-Curie 14, 50-383 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8111-1840","authenticated-orcid":false,"given":"Battal","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences, Selcuk University, Alaeddin Keykubad Campus, Yeni Istanbul Street, No:335, Konya 42130, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bonel-Ayuso, D.P., Pineda-Pampliega, J., Martinez-Ales\u00f3n Garc\u00eda, P., Fernandez-Muela, M., de la Fuente, J., Garcia Fernandez, P.M., and Redondo, B.I. 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