{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:38:30Z","timestamp":1786711110197,"version":"build-2736575974"},"reference-count":41,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2024LHMS03055"],"award-info":[{"award-number":["2024LHMS03055"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Program of Inner Mongolia Autonomous Region","award":["2026SYFDZ0272"],"award-info":[{"award-number":["2026SYFDZ0272"]}]},{"name":"China Agriculture Research System","award":["CARS-38"],"award-info":[{"award-number":["CARS-38"]}]},{"award":["CARS-38"],"award-info":[{"award-number":["CARS-38"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Peste des petits ruminants virus (PPRV) causes a highly fatal disease that severely impacts small ruminant production and global food security. This study aimed to develop a rapid, user-friendly colloidal gold immunochromatographic test strip for detecting PPRV-specific antibodies using a double-antigen sandwich format. Bioinformatic analysis using DNASTAR Protean was performed to predict candidate antigenic regions in the PPRV H and N proteins. Three predicted candidate regions from each protein were selected and incorporated into the design of the recombinant fusion antigen PPRV-H3N3EP. The recombinant antigen was expressed in E. coli, purified, and refolded to obtain a final concentration of 8.52 mg\/mL. The strip was assembled with colloidal gold-labeled fusion protein as the detection probe and unlabeled protein coated on the test line, plus an independent mouse IgG\/goat anti-mouse IgG control system. Performance evaluation showed that the results were readable within 10\u201315 min. The strip showed satisfactory analytical sensitivity and cross-reactivity performance, consistent qualitative results in within-batch repeatability testing, and preliminary short-term storage stability. In a comparative evaluation using sheep serum samples and a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit, the overall agreement reached 97.9%. Collectively, the constructed PPRV-H3N3EP antigen enabled a simple, rapid, and reliable strip assay suitable for field detection of PPRV antibodies and post-vaccination monitoring, while also providing a methodological reference for developing antibody tests for other pathogens.<\/jats:p>","DOI":"10.3390\/ani16162545","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:57:46Z","timestamp":1786708666000},"page":"2545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Colloidal Gold Immunochromatographic Test Strip for PPRV Antibody Detection Based on Antigenic Epitope-Derived Recombinant Protein"],"prefix":"10.3390","volume":"16","author":[{"given":"Shenyuan","family":"Wang","sequence":"first","affiliation":[{"name":"Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Han","sequence":"additional","affiliation":[{"name":"College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhao","family":"Sun","sequence":"additional","affiliation":[{"name":"Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-4673","authenticated-orcid":false,"given":"Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbin","family":"Liu","sequence":"additional","affiliation":[{"name":"China-Mongolia Biomacromolecule Application \u201cBelt and Road\u201d Joint Laboratory, Hohhot 010018, China"},{"name":"Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kumbe, A., Negussie, H., Getachew, Y., Alemu, B., Alemayehu, G., Girma, S., Sibhatu, D., Emiyu, K., Waktole, H., and Leta, S. 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