{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T05:23:52Z","timestamp":1787117032450,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People\u2019s Republic of China","doi-asserted-by":"crossref","award":["2024YFD1300701"],"award-info":[{"award-number":["2024YFD1300701"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2024YFD1300701"],"award-info":[{"award-number":["2024YFD1300701"]}],"id":[{"id":"https:\/\/ror.org\/027s68j25","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention","award":["ABDC-b202502"],"award-info":[{"award-number":["ABDC-b202502"]}]},{"DOI":"10.13039\/501100011823","name":"Department of Education of Guangxi Zhuang Autonomous Region","doi-asserted-by":"crossref","award":["2024KY1248"],"award-info":[{"award-number":["2024KY1248"]}],"id":[{"id":"10.13039\/501100011823","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Guangxi Bama miniature pigs are a highly inbred population developed from Bama Xiang pigs and exhibit a stable \u201ctwo-end black\u201d skin phenotype, making them a useful model for studying regional skin pigmentation. However, the developmental timing and molecular basis of this color difference remain unclear. In this study, black and white skin samples were collected from the posterior head\/neck region at six developmental stages: embryonic days 65, 90, and 105, birth, and postnatal days 30 and 180. Histological examination and strand-specific RNA sequencing showed that developmental stage was the major factor shaping skin gene expression, whereas black\u2013white skin differences varied across stages. Transcriptomic differences were already detectable at embryonic day 65, although visible regional differences were not obvious. Pigmentation-associated genes, including DCT, SLC24A5, TRPM1, and TYRP1, showed black-skin-biased expression during embryonic development, especially at embryonic day 105. Histological analysis also revealed progressive skin maturation and qualitative differences in darkly stained structures between black and white skin. These findings suggest that molecular differences between black and white skin emerge during embryonic development and persist after birth, providing resources and candidate genes for studying regional pigmentation in Bama miniature pigs.<\/jats:p>","DOI":"10.3390\/ani16162565","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:35:08Z","timestamp":1786984508000},"page":"2565","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histological and Strand-Specific Transcriptomic Analyses Reveal Developmental Dynamics and Pigmentation-Associated Candidate Genes in Black and White Skin Regions of Bama Miniature Pigs"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9818-3217","authenticated-orcid":false,"given":"Lingli","family":"Feng","sequence":"first","affiliation":[{"name":"Liangzhu Laboratory, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 311100, China"},{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Xia","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry, Guangxi Vocational University of Agriculture, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongrong","family":"Deng","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3576-3219","authenticated-orcid":false,"given":"Alida","family":"Abla","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiafu","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinning","family":"Liang","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5533-2107","authenticated-orcid":false,"given":"Jing","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1038\/s41588-019-0439-2","article-title":"Genome-wide association study identifies eight risk loci and implicates metabo-psychiatric origins for anorexia nervosa","volume":"51","author":"Watson","year":"2019","journal-title":"Nat. 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