{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:23:48Z","timestamp":1787142228776,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272918"],"award-info":[{"award-number":["32272918"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32272918"],"award-info":[{"award-number":["32272918"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The enzyme-treated soybean protein (ETSP) could improve their application as an ingredient and substitute for fishmeal (FM) in aquafeeds. In the present research, an eight-week feeding trial was carried out to investigate the effects of using ETSP as a particle substitution in FM on growth performance, serum biochemical indices, antioxidant, intestinal barrier integrity and relative mRNA expression levels in largemouth bass (Micropterus salmoides). A cohort of 450 fish (15.52 \u00b1 0.04 g\/fish) were randomly divided into five groups with five replacement levels of ETSP: 0, 12.5, 25.0, 37.5 and 50.0% (control, H2-12.5, H2-25.0, H2-37.5 and H2-50.0). Results showed that final body weight (FBW), weight gain (WG), and specific growth rate (SGR) reach the highest value, and the feed conversion ratio (FCR) reaches lowest in H2-25.0 group. The activities of total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were remarkably increased by replacing FM with ETSP at certain level, while malondialdehyde (MDA) concentration significantly decreased (p &lt; 0.05). Higher levels of total protein (TP) and albumin (ALB) as well as lower activity of aspartate aminotransferase (AST) were detected with ETSP replacement. Histological examination revealed that villus height in mid-intestine was the highest, while this factor in pyloric caeca was not affected. The activity of diamine oxidase (DAO) and the contents of D-lactate (D-Lac) and lipopolysaccharide (LPS) in serum were gradually decreased when replacing FM with ETSP in the diets. In addition, replacement of FM with ETSP at 25% improved intestinal barrier function by upregulating gene expression related to the tight junctions (occludin, claudins, ZO-1 and ZO-2) and anti-inflammatory cytokines (IL-10) while downregulating pro-inflammatory factors (IL-8 and TNF\u03b1). Moreover, proper amount of ETSP instead of FM can upregulate mRNA expression of LAT1, PEPT1 and PEPT2 in the intestine. In conclusion, at the fishmeal level used in the diet tested in the present study, 25% replacement is the most beneficial for juvenile largemouth bass.<\/jats:p>","DOI":"10.3390\/ani16162589","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:04:12Z","timestamp":1787137452000},"page":"2589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Replacing Dietary Fishmeal with Enzyme-Treated Soybean Protein on Growth Performance, Serum Biochemical Indices, Antioxidant, Digestive Enzyme Activities, Intestinal Barrier Integrity and Relative mRNA Expression Levels of Juvenile Largemouth Bass (Micropterus salmoides)"],"prefix":"10.3390","volume":"16","author":[{"given":"Xia","family":"Dong","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Guo","sequence":"additional","affiliation":[{"name":"Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8639-0745","authenticated-orcid":false,"given":"Hongsen","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Xiong","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1571-9818","authenticated-orcid":false,"given":"Luyang","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihe","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3320-2381","authenticated-orcid":false,"given":"Yulan","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Li","sequence":"additional","affiliation":[{"name":"Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1569-9953","authenticated-orcid":false,"given":"Honggang","family":"Huang","sequence":"additional","affiliation":[{"name":"Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, No. 68, Xuefunan Road, Wuhan 430023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","first-page":"404","article-title":"Advances in animal and plant protein sources in place of fish meal","volume":"29","author":"Zhou","year":"2005","journal-title":"J. Fish. China"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"736185","DOI":"10.1016\/j.aquaculture.2020.736185","article-title":"Effects of fishmeal replacement with composite mixture of shrimp hydrolysate and plant proteins on growth performance, feed utilization, and target of rapamycin pathway in largemouth bass, Micropterus salmoides","volume":"533","author":"Li","year":"2021","journal-title":"Aquaculture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.oneear.2019.10.018","article-title":"The future of aquatic protein: Implications for protein sources in aquaculture diets","volume":"1","author":"Hua","year":"2019","journal-title":"One Earth"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.plantsci.2018.03.030","article-title":"The promise and limits for enhancing sulfur-containing amino acid content of soybean seed","volume":"272","author":"Krishnan","year":"2018","journal-title":"Plant Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s10499-010-9359-y","article-title":"Effect of replacing fish meal with soybean meal on growth, feed utilization and nitrogen and phosphorus excretion on rainbow trout (Oncorhynchus mykiss)","volume":"19","author":"Yang","year":"2011","journal-title":"Aquac. Int."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Liang, H., Ren, M., Zhang, L., Mi, H., Yu, H., Huang, D., Gu, J., and Teng, T. (2024). Excessive replacement of fish meal by soy protein concentrate resulted in inhibition of growth, nutrient metabolism, antioxidant capacity, immune capacity, and intestinal development in juvenile largemouth bass (Micropterus salmoides). Antioxidants, 13.","DOI":"10.3390\/antiox13070809"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.fsi.2008.02.013","article-title":"Soybean meal induces intestinal inflammation in common carp (Cyprinus carpio L.)","volume":"25","author":"Schrama","year":"2008","journal-title":"Fish. Shellfish Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bai, X.L., Mi, H.F., Huang, D.Y., Liang, H.L., Shan, W., Ren, M.C., Zhang, L., and Teng, T. (2025). Effects of replacing fishmeal with enzymatically hydrolyzed pork bone meal (EHPBM) on growth, antioxidant capacity, and nutritional metabolism in Micropterus salmoides. Animals, 15.","DOI":"10.3390\/ani15233359"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"738896","DOI":"10.1016\/j.aquaculture.2022.738896","article-title":"Dietary protein hydrolysate effects on growth, digestive enzymes activity, and expression of genes related to amino acid transport and metabolism of larval snakehead (Channa argus)","volume":"563","author":"Sheng","year":"2023","journal-title":"Aquaculture"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100141","DOI":"10.1016\/j.fochx.2021.100141","article-title":"Assessment the flavor of soybean meal hydrolyzed with Alcalase enzyme under different hydrolysis conditions by E-nose, E-tongue and HS-SPME-GC\u2013MS","volume":"12","author":"Weng","year":"2021","journal-title":"Food Chem. X"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10844","DOI":"10.1021\/acs.jafc.7b05026","article-title":"Small peptides isolated from enzymatic hydrolyzate of fermented soybean meal promote endothelium-independent vasorelaxation and ACE inhibition","volume":"65","author":"Wang","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1007\/s10695-017-0400-1","article-title":"Supplementation of enzyme-treated soy protein saves dietary protein and promotes digestive and absorptive ability referring to TOR signaling in juvenile fish","volume":"43","author":"Xiao","year":"2017","journal-title":"Fish Physiol. Biochem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.aquaculture.2008.03.016","article-title":"Taxonomic status and genetic diversity of cultured largemouth bass Micropterus salmoides in China","volume":"278","author":"Bai","year":"2008","journal-title":"Aquaculture"},{"key":"ref_14","first-page":"1","article-title":"Biological synopsis of largemouth bass (Micropterus salmoides)","volume":"2884","author":"Brown","year":"2009","journal-title":"Can. Manuscr. Rep. Fish. Aquat. Sci."},{"key":"ref_15","unstructured":"Ministry of Agriculture and Rural Affairs Fisheries Administration Bureau, and National Aquatic Product Technology Extension Station, Fisheries, C.S.O (2023). 2023 China Fishery Statistical Yearbook."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Xian, Y.L., Wang, F.B., Zou, Z.C., Wen, J.Y., Chen, Y.P., Zhang, J.P., and Wang, Y. (2026). Optimizing feeding regimens with differential protein levels to enhance growth and health in juvenile largemouth bass (Micropterus salmoides). Animals, 16.","DOI":"10.3390\/ani16101542"},{"key":"ref_17","unstructured":"Horwitz, W. (1997). Official Methods of Analysis of AOAC International. Volume, I.; Agricultural Chemicals, Contaminants, Drugs, AOAC International."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipids from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.ijbiomac.2022.10.185","article-title":"Dietary chitosan moderates the growth rate, antioxidant activity, immunity, intestinal morphology and resistance against Aeromonas hydrophila of juvenile hybrid sturgeon (Acipenser baerii\u2640 \u00d7 Acipenser schrenckii\u2642)","volume":"224","author":"Xu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, M., Wang, Y.F., Li, X., Yin, Y., Zhang, X.W., Wu, S., Wang, H.Q., and Zhao, Y.R. (2024). Effects of dietary ursolic acid on growth performance and intestinal health of largemouth bass (Micropterus salmoides). Animals, 14.","DOI":"10.2139\/ssrn.4879980"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Uyisenga, A., Liang, H., Ren, M., Huang, D., Xue, C., Yin, H., and Mi, H. (2023). The effects of replacing fish meal with enzymatic soybean meal on the growth performance, whole-body composition, and health of juvenile gibel carp (Carassius auratus gibelio). Fishes, 8.","DOI":"10.3390\/fishes8080423"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1111\/anu.13134","article-title":"Effects of different dietary levels of soybean protein hydrolysates on the growth performance, antioxidant capacity and relative mRNA expression levels of juvenile hybrid grouper (Epinephelus fuscoguttatus\u2640 \u00d7 Epinephelus lanceolatus\u2642)","volume":"26","author":"Liang","year":"2020","journal-title":"Aquac. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1111\/anu.13297","article-title":"Substitution of fish meal with enzyme-treated soybean in diets for juvenile largemouth bass (Micropterus salmoides)","volume":"27","author":"Liu","year":"2021","journal-title":"Aquac. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lin, H., Tan, B.P., Ray, G.W., Zeng, M., Li, M., Chi, S.Y., and Yang, Q.H. (2022). A challenge to conventional fish meal: Effects of soy protein peptides on growth, histomorphology, lipid metabolism and intestinal health for juvenile pompano Trachinotus ovatus. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.815323"},{"key":"ref_25","first-page":"JFAD-143","article-title":"Direct substitution of fishmeal with bioprocessed soybean meal in brown trout diets","volume":"2018","author":"Voorhees","year":"2018","journal-title":"J. Fish. Aqua. Dev."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ma, C., Xiao, Y., Xiong, S., Yu, J., Chen, W.Y., He, Y., Chen, Y., and Lin, S.M. (2026). Dietary supplementation with organic acids improves production performance and intestinal health of largemouth bass. Animals, 16.","DOI":"10.3390\/ani16081198"},{"key":"ref_27","first-page":"57","article-title":"The role of amino acid transporters in nutrition","volume":"16","author":"Poncet","year":"2013","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"19738","DOI":"10.1074\/jbc.274.28.19738","article-title":"Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids","volume":"274","author":"Ma","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"19745","DOI":"10.1074\/jbc.274.28.19745","article-title":"Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity","volume":"274","author":"Segawa","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/naaq.10285","article-title":"Effects of fish meal replacement with composite mixture of soybean protein hydrolysates and other plant proteins on growth performance, antioxidant capacity, and target of rapamycin pathway in largemouth bass","volume":"85","author":"Wang","year":"2023","journal-title":"N. Am. J. Aquac."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.aninu.2025.07.005","article-title":"Evaluating soybean protein concentrate hydrolysate as a fishmeal substitute in the diet for mandarin fish (Siniperca chuatsi): Based on growth, intestinal health, flesh quality and nutrient metabolism","volume":"23","author":"Sun","year":"2025","journal-title":"Anim. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.cbpa.2010.06.162","article-title":"The effect of plant protein-based diet supplemented with dipeptide or free amino acids on digestive tract morphology and PepT1 and PepT2 expressions in common carp (Cyprinus carpio L.)","volume":"157","author":"Ostaszewska","year":"2010","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Yang, X., Zhao, X., Wang, G., Dong, X., Yang, Q., Liu, H., Zhang, S., Tan, B., and Chi, S. (2022). Improvement of hybrid grouper (Epinephelus fuscoguttatus\u2640 \u00d7 E. lanceolatus\u2642) by enzyme-digested poultry by-product: Growth performance, amino acid and peptide transport capacity, and intestinal morphology. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.955734"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1007\/s11160-012-9266-6","article-title":"The effect of dietary dipeptide lysine\u2013glycine on growth, muscle proteins, and intestine PepT1 gene expression in juvenile yellow perch","volume":"22","author":"Kwasek","year":"2012","journal-title":"Rev. Fish Biol. Fish."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s10499-025-02204-4","article-title":"Enzyme-treated soybean protein concentrate is a highly effective plant protein for larval largemouth bass (Micropterus salmoides)","volume":"33","author":"Tao","year":"2025","journal-title":"Aquac. Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1016\/j.fsi.2018.12.028","article-title":"Serum biochemistry, liver histology and transcriptome profiling of bighead carp Aristichthys nobilis following different dietary protein levels","volume":"86","author":"Sun","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1006\/eesa.1998.1714","article-title":"Biomarker assessment of the effects of coal strip-mine contamination on channel catfish","volume":"41","author":"Martin","year":"1998","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"100938","DOI":"10.1016\/j.aqrep.2021.100938","article-title":"Partial substitution of fish meal by Clostridium autoethanogenum protein in the diets of juvenile largemouth bass (Micropterus salmoides)","volume":"22","author":"Zhu","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1007\/s00580-012-1532-4","article-title":"Hematological and serum biochemical indices changes induced by replacing fish meal with plant protein (sesame oil cake and corn gluten) in the Great sturgeon (Huso huso)","volume":"22","author":"Jahanbakhshi","year":"2013","journal-title":"Comp. Clin. Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.aquaculture.2013.07.022","article-title":"Potential use of macro-algae Gracilaria lemaneiformis in diets for the black sea bream, Acanthopagrus schlegelii, juvenile","volume":"412","author":"Xuan","year":"2013","journal-title":"Aquaculture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/anu.12355","article-title":"Effects of replacing plant proteins with rubber seed meal on growth, nutrient utilization and blood biochemical parameters of tilapia (Oreochromis niloticus \u00d7 O. aureus)","volume":"23","author":"Deng","year":"2017","journal-title":"Aquac. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/anu.13173","article-title":"Dietary calcium requirement of red swamp crayfish (Procambarus clarkia)","volume":"27","author":"Xu","year":"2021","journal-title":"Aquac. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.cbpc.2003.12.003","article-title":"Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans (Teleostei: Siluriformes) in response to diet composition","volume":"137","author":"Lundstedt","year":"2004","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.cbpa.2006.12.037","article-title":"Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae","volume":"147","author":"Kotzamanis","year":"2007","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"736269","DOI":"10.1016\/j.aquaculture.2020.736269","article-title":"The effects of chitosan-vitamin C nanocomposite supplementation on the growth performance, antioxidant status, immune response, and disease resistance of Nile tilapia (Oreochromis niloticus) fingerlings","volume":"534","author":"Ibrahim","year":"2021","journal-title":"Aquaculture"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lan, R.X., Chang, Q.Q., An, L.L., and Zhao, Z.H. (2020). Dietary Supplementation with chitosan oligosaccharides alleviates oxidative stress in rats challenged with hydrogen peroxide. Animals, 10.","DOI":"10.3390\/ani10010055"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.fsi.2018.02.042","article-title":"Antioxidant defense system, immune response and erythron profile modulation in gold fish, Carassius auratus, after acute manganese treatment","volume":"76","author":"Aliko","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"ref_48","first-page":"102152","article-title":"A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides","volume":"22","author":"Zhao","year":"2025","journal-title":"J. Agric. Food Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1111\/are.14016","article-title":"Effect of dietary enzyme-treated soy protein on the immunity and antioxidant status in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)","volume":"50","author":"Xiao","year":"2019","journal-title":"Aquac. Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Shen, J.J., Liu, H.X., Wang, M.R., Lu, B., Ke, K., Wei, Y.Y., Gao, F., Wang, Q.Z., Huang, S., and Ma, Y.Q. (2025). Effects of brown algae (Laminaria japonica) extract on growth performance, immune function and intestinal health of largemouth bass (Micropterus salmoides). Animals, 15.","DOI":"10.3390\/ani15050622"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"101097","DOI":"10.1016\/j.aqrep.2022.101097","article-title":"Effects of dietary galactooligosaccharide on growth, antioxidants, immunity, intestinal morphology and disease resistance against Aeromons hydrophila in juvenile hybrid sturgeon (Acipenser baerii\u2640 \u00d7 A. schrenckii\u2642)","volume":"23","author":"Xu","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Liu, Y., Zhang, Y., Fan, J., Zhou, H., Huang, H., Cao, Y., Jiang, W., Zhang, W., Deng, J., and Tan, B. (2022). Effects of different viscous guar gums on growth, apparent nutrient digestibility, intestinal development and morphology in juvenile largemouth bass, Micropterus salmoides. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.927819"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"299S","DOI":"10.1093\/ajcn\/55.1.299s","article-title":"Physiology and pathophysiology of intestinal absorption","volume":"55","author":"Caspary","year":"1992","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1111\/anu.12797","article-title":"Assessing the impact of Bacillus strains mixture probiotic on water quality, growth performance, blood profile and intestinal morphology of Nile tilapia, Oreochromis niloticus","volume":"24","author":"Elsabagh","year":"2018","journal-title":"Aquac. Nutr."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Xu, Y., Wu, C., Wang, P., Han, X., Yang, J., and Zhai, S. (2024). Effects of dietary inclusion of enzymatically hydrolyzed compound soy protein on the growth performance and intestinal health of juvenile American eels (Anguilla rostrata). Animals, 14.","DOI":"10.3390\/ani14213096"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1111\/jpn.13094","article-title":"Montmorillonite improved the intestinal mucosal barrier functions of laying hens in late production","volume":"103","author":"Chen","year":"2019","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1172\/JCI110881","article-title":"Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat","volume":"71","author":"Luk","year":"1983","journal-title":"J. Clin. Investig."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1128\/jcm.27.3.385-388.1989","article-title":"D-lactic acid measurements in the diagnosis of bacterial infections","volume":"27","author":"Smith","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Du, M., Chen, Y., Wang, S., Zhao, H., Wen, C., and Zhou, Y. (2022). Effects of dietary palygorskite supplementation on the growth performance, oxidative status, immune function, intestinal barrier and cecal microbial community of broilers. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.985784"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zheng, J., Yang, P., Dai, J., Yu, G., Ou, W., Xu, W., Mai, K., and Zhang, Y. (2020). Dynamics of intestinal inflammatory cytokines and tight junction proteins of turbot (Scophthalmus maximus L.) during the development and recovery of enteritis induced by dietary \u03b2-conglycinin. Front. Mar. Sci., 7.","DOI":"10.3389\/fmars.2020.00198"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Li, C., Li, X., Li, P., Wei, B., Zhang, C., Zhu, X., and Zhang, J. (2022). Sodium humate alters the intestinal microbiome, short-chain fatty acids, eggshell ultrastructure, and egg performance of old laying hens. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.986562"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e1373208","DOI":"10.1080\/21688370.2017.1373208","article-title":"Intestinal barrier and gut microbiota: Shaping our immune responses throughout life","volume":"5","author":"Takiishi","year":"2017","journal-title":"Tissue Barriers"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1136\/gut.2007.140806","article-title":"Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: Involvement of soluble mediators","volume":"58","author":"Piche","year":"2009","journal-title":"Gut"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2589\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T11:23:39Z","timestamp":1787138619000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":63,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ani16162589"],"URL":"https:\/\/doi.org\/10.3390\/ani16162589","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}