{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:28:34Z","timestamp":1787225314819,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study investigated circulating tight junction (TJ) proteins (Zonula Occludens-1 [ZO-1], Occludin, Claudin-3) and Glucagon-Like Peptide-2 (GLP-2) concentrations across varying clinical severities of neonatal calf diarrhea. Thirty-six calves\u2014categorized by Systemic Inflammatory Response Syndrome (SIRS) criteria into SIRS-positive (n = 12), SIRS-negative (n = 11), and healthy control (n = 13) groups\u2014were evaluated via ELISA. In SIRS-negative calves, serum ZO-1 levels were significantly lower than in healthy controls (p = 0.0276) and positively correlated with leukocyte counts (r = 0.64). Conversely, SIRS-positive calves exhibited significantly higher GLP-2 levels compared with SIRS-negative calves (p = 0.0040), whereas serum ZO-1 levels did not differ significantly from healthy controls. Significant positive correlations (|r| &gt; 0.50, p &lt; 0.05) were identified between GLP-2 and TJ proteins within the SIRS-positive cohort. In conclusion, circulating GLP-2 and ZO-1 may serve as potential candidate biomarkers for monitoring intestinal barrier profiles and clinical severity in diarrheic calves, though further validation in larger prospective cohorts is warranted.<\/jats:p>","DOI":"10.3390\/ani16162607","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:55:18Z","timestamp":1787223318000},"page":"2607","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Intestinal Barrier Biomarkers and Glucagon-like Peptide-2 in SIRS-Positive and SIRS-Negative Diarrheic Calves"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4779-0893","authenticated-orcid":false,"given":"Adem","family":"\u015eahan","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, \u015eanl\u0131urfa 63200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4246-8181","authenticated-orcid":false,"given":"Sercan H\u00fcseyin","family":"Bayendur","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7473-5163","authenticated-orcid":false,"given":"Canberk","family":"Bal\u0131k\u00e7\u0131","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, \u015eanl\u0131urfa 63200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet Cihat","family":"Tun\u00e7","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abuzer","family":"Acar","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jessop, E., Li, L., Renaud, D.L., Verbrugghe, A., Macnicol, J., Gamsj\u00e4ger, L., and Gomez, D.E. 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