{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T17:23:43Z","timestamp":1787246623441,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kemin Industries"},{"id":[{"id":"https:\/\/ror.org\/01jh73d13","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated the effects of supplementation with Toxfin\u2122 Dry, a mineral adsorbent composed of bentonite and sepiolite, on productive performance, egg quality, intestinal morphometry, serum biochemical parameters, antioxidant status, and sphingolipid metabolism of laying hens challenged with T-2 toxin and fumonisin. A total of 480 Hy-Line W-36 laying hens at 25 weeks of age were distributed in a completely randomized design with six treatments and eight replicates of 10 birds each, over 112 days divided into four 28-day cycles. Treatments consisted of a control diet, either uncontaminated or contaminated with 2 mg\/kg T-2 toxin and 60 mg\/kg fumonisin B1, with or without 1 kg\/ton Toxfin\u2122 Dry. Mycotoxin challenge reduced egg production (p &lt; 0.0001), egg weight (p &lt; 0.0001), egg mass (p &lt; 0.0001), feed intake (p &lt; 0.0001), eggshell strength (p &lt; 0.0001) and thickness (p &lt; 0.0001), Haugh unit (p &lt; 0.0001), and villus ratio (p &lt; 0.0001), while increasing inflammatory and hepatic biomarkers, malondialdehyde levels, and the sphinganine ratio, with more pronounced effects under combined contamination. Supplementation with Toxfin\u2122 Dry mitigated most adverse effects, with responses approaching those of the control group. The adsorbent reduces intestinal mycotoxin bioavailability and helps maintain performance and physiological homeostasis in laying hens.<\/jats:p>","DOI":"10.3390\/ani16162613","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:05:19Z","timestamp":1787241919000},"page":"2613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Toxfin in Mitigating Mycotoxin Effects in Laying Hens"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2796-1675","authenticated-orcid":false,"given":"Adiel Vieira de","family":"Lima","sequence":"first","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3425-9972","authenticated-orcid":false,"given":"Jos\u00e9 de Arimat\u00e9ia de Freitas","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9355-7657","authenticated-orcid":false,"given":"Carlos Henrique do","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Beatriz","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7215-6325","authenticated-orcid":false,"given":"Paloma Eduarda Lopes de","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto de Ara\u00fajo","family":"Brito Filho","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raiane dos Santos","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luayne Morais","family":"Correa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8226-8606","authenticated-orcid":false,"given":"Ricardo Rom\u00e3o","family":"Guerra","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1123-086X","authenticated-orcid":false,"given":"Edijanio Galdino da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6730-0209","authenticated-orcid":false,"given":"Apol\u00f4nio Gomes","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Cristina Ramos Rezende","family":"Arrais","sequence":"additional","affiliation":[{"name":"Kemin Animal Nutrition & Health Division, Latin America, Rua Krebsfer 736, Valinhos 13279-450, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3881-2373","authenticated-orcid":false,"given":"Matheus Carlos Romeiro","family":"Miranda","sequence":"additional","affiliation":[{"name":"Kemin Animal Nutrition & Health Division, Latin America, Rua Krebsfer 736, Valinhos 13279-450, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Pinto","family":"Piccoli","sequence":"additional","affiliation":[{"name":"Kemin Animal Nutrition & Health Division, Latin America, Rua Krebsfer 736, Valinhos 13279-450, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4075-1792","authenticated-orcid":false,"given":"Fernando Guilherme Perazzo","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Federal University of Para\u00edba, PB-079 Highway, Areia 58397-000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kovalsky, P., Kos, G., N\u00e4hrer, K., Schwab, C., Jenkins, T., Schatzmayr, G., Sulyok, M., and Krska, R. (2016). Co-occurrence of regulated, masked and emerging mycotoxins and secondary metabolites in finished feed and maize\u2014An extensive survey. Toxin, 8.","DOI":"10.3390\/toxins8120363"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"103471","DOI":"10.1016\/j.psj.2024.103471","article-title":"Pathological consequences, metabolism and toxic effects of trichothecene T-2 toxin in poultry","volume":"103","author":"Mackei","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1194\/jlr.S093815","article-title":"Ceramide synthase inhibition by fumonisins: A perfect storm of perturbed sphingolipid metabolism, signaling, and disease","volume":"60","author":"Riley","year":"2019","journal-title":"J. Lip. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1080\/00439339.2024.2347307","article-title":"Mycotoxicosis and oxidative stress in poultry: Pathogenesis and therapeutic insights","volume":"80","author":"Alam","year":"2024","journal-title":"World. Poult. Sci. J."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Liew, W.P.P., and Mohd-Redzwan, S. (2018). Mycotoxin: Its impact on gut health and microbiota. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00060"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.3390\/toxins7041253","article-title":"Dose-dependent effects on sphingoid bases and cytokines in chickens fed diets prepared with Fusarium verticillioides culture material containing fumonisins","volume":"7","author":"Grenier","year":"2015","journal-title":"Toxin"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"skac328","DOI":"10.1093\/jas\/skac328","article-title":"The efficacy of mycotoxin binders to control mycotoxins in feeds and the potential risk of interactions with nutrient: A review","volume":"100","author":"Kihal","year":"2022","journal-title":"J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"106874","DOI":"10.1016\/j.clay.2023.106874","article-title":"From old to new inorganic materials for advanced applications: The paradigmatic example of the sepiolite clay mineral","volume":"235","author":"Ruiz","year":"2023","journal-title":"Appl. Clay Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1590\/S0100-204X2004000500014","article-title":"Comparison of methods to measure the specific gravity of laying hen eggs","volume":"39","author":"Freitas","year":"2004","journal-title":"Pesq. Agropec. Bras."},{"key":"ref_10","first-page":"572","article-title":"The Haugh unit for measuring egg quality","volume":"43","author":"Haugh","year":"1937","journal-title":"U. S. Egg Poult. Mag."},{"key":"ref_11","first-page":"e11771","article-title":"Biochemical parameters of laying hens and japanese quails (Coturnix coturnix japonica)","volume":"23","author":"Lima","year":"2025","journal-title":"Obs. Econ. Latinoam."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"38","DOI":"10.4274\/tpd.galenos.2022.07088","article-title":"The Importance of Antioxidant Enzymes and Oxidative Stress in Human Fascioliasis","volume":"47","author":"Cengiz","year":"2023","journal-title":"Turk. Parazit. Derg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.3382\/ps.2012-02489","article-title":"Individual and combined effects of Salmonella typhimurium lipopolysaccharide and fumonisin B1 in broiler chickens","volume":"11","author":"Rauber","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113044","DOI":"10.1016\/j.fct.2022.113044","article-title":"Toxic mechanisms of the trichothecenes T-2 toxin and deoxynivalenol on protein synthesis","volume":"164","author":"Li","year":"2022","journal-title":"Food. Chem. Toxicol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e10012","DOI":"10.1016\/j.heliyon.2022.e10012","article-title":"An update on T2-toxins: Metabolism, immunotoxicity mechanism and human assessment exposure of intestinal microbiota","volume":"8","author":"Zhang","year":"2022","journal-title":"Heliyon"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/19960401NT2","article-title":"Evidence for disruption of sphingolipid metabolism as a contributing factor in the toxicity and carcinogenicity of fumonisins","volume":"4","author":"Riley","year":"1996","journal-title":"Nat. Toxins"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e22397","DOI":"10.1002\/jbt.22397","article-title":"Fumonisin B1 damages the barrier functions of porcine intestinal epithelial cells in vitro","volume":"33","author":"Chen","year":"2019","journal-title":"J. Biochem. Molec. Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1093\/jn\/117.3.605","article-title":"Control of energy balance during egg production in the laying hen","volume":"117","author":"Scanes","year":"1987","journal-title":"J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"860","DOI":"10.4315\/0362-028X-66.5.860","article-title":"Aflatoxin B1 and Clinoptilolite in Feed for Laying Hens: Effects on Egg Quality, Mycotoxin Residues in Livers, and Hepatic Mixed-Function Oxygenase Activities","volume":"66","author":"Rizzi","year":"2003","journal-title":"J. Food. Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1093\/ps\/84.6.825","article-title":"Mannanoligosaccharides and aflatoxin B1 in feed for laying hens: Effects on egg quality, aflatoxins B1 and M1 residues in eggs, and aflatoxin B1 levels in liver","volume":"84","author":"Zaghini","year":"2005","journal-title":"Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.3382\/ps.2012-02136","article-title":"Mycotoxin-contaminated diets and deactivating compound in laying hens: 1. Effects on performance characteristics and relative organ weight","volume":"91","author":"Lee","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_22","unstructured":"Surai, P. (2002). Natural Antioxidants in Avian Nutrition and Reproduction, Nottingham University Press. Available online: https:\/\/www.feedfood.co.uk\/download\/Imm_my_2002a.pdf."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0378-4274(01)00360-5","article-title":"Mycotoxin detoxication of animal feed by different adsorbents","volume":"122","author":"Huwig","year":"2001","journal-title":"Toxicol. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/BF02946021","article-title":"Investigation of various adsorbents for their ability to bind aflatoxin B1","volume":"23","author":"Vekiru","year":"2007","journal-title":"Mycotox. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3390\/toxins5020396","article-title":"Modulation of intestinal functions following mycotoxin ingestion: Meta-analysis of published experiments in animals","volume":"5","author":"Grenier","year":"2013","journal-title":"Toxin"},{"key":"ref_26","first-page":"167","article-title":"Estabelecimento de escores histopatol\u00f3gicos de les\u00e3o hep\u00e1tica e determina\u00e7\u00e3o dos valores normais das enzimas aspartato aminotransferase e creatina quinase em frangos de corte","volume":"32","author":"Moraes","year":"2018","journal-title":"Acta Sci. Vet."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Riva, F., Eckersall, P.D., Chadwick, C.C., Chadwick, L.C., McKeegan, D.E.F., Peinado-Izaguerri, J., Bruggeman, G., Hermans, D., McLaughlin, M., and Bain, M. (2024). Identification of novel biomarkers of acute phase response in chickens challenged with Escherichia coli lipopolysaccharide endotoxin. BMC Vet. Res., 20.","DOI":"10.1186\/s12917-024-04062-3"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Shanmugasundaram, R., Kappari, L., Pilewar, M., Jones, M.K., Olukosi, O.A., Pokoo-Aikins, A., Applegate, T.J., and Glenn, A.E. (2025). Exposure to Subclinical Doses of Fumonisins, Deoxynivalenol, and Zearalenone Affects Immune Response, Amino Acid Digestibility, and Intestinal Morphology in Broiler Chickens. Toxin, 17.","DOI":"10.3390\/toxins17010016"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103141","DOI":"10.1016\/j.psj.2023.103141","article-title":"Heat stress and poultry production: A comprehensive review","volume":"102","author":"Oluwagbenga","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kappari, L., Applegate, T.J., Glenn, A.E., Bakre, A., and Shanmugasundaram, R. (2025). Early Biomarkers for Detecting Subclinical Exposure to Fumonisin B1, Deoxynivalenol, and Zearalenone in Broiler Chickens. Toxin, 17.","DOI":"10.3390\/toxins17010001"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s00204-015-1604-8","article-title":"Fumonisins: Oxidative stress-mediated toxicity and metabolism in vivo and in vitro","volume":"90","author":"Wang","year":"2016","journal-title":"Arch. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Surai, P.F., Kochish, I.I., Fisinin, V.I., and Kidd, M.T. (2019). Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidant, 8.","DOI":"10.3390\/antiox8070235"},{"key":"ref_33","first-page":"31","article-title":"Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal","volume":"360438","author":"Ayala","year":"2014","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Cuccato, M., Amminikutty, N., Spalenza, V., Conte, V., Bagatella, S., Greco, D., D\u2019Ascanio, V., Gai, F., Schiavone, A., and Avantaggiato, G. (2025). Innovative Mycotoxin Detoxifying Agents Decrease the Absorption Rate of Aflatoxin B1 and Counteract the Oxidative Stress in Broiler Chickens Exposed to Low Dietary Levels of the Mycotoxin. Toxin, 17.","DOI":"10.3390\/toxins17020082"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tardieu, D., Matard-Mann, M., Nyvall Coll\u00e9n, P., and Guerre, P. (2021). Strong Alterations in the Sphingolipid Profile of Chickens Fed a Dose of Fumonisins Considered Safe. Toxin, 13.","DOI":"10.3390\/toxins13110770"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"14486","DOI":"10.1016\/S0021-9258(18)98712-0","article-title":"Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme","volume":"266","author":"Wang","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Rohrhofer, J., Zwirzitz, B., Selberherr, E., and Untersmayr, E. (2021). The Impact of Dietary Sphingolipids on Intestinal Microbiota and Gastrointestinal Immune Homeostasis. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.635704"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.toxicon.2017.12.041","article-title":"Fumonisin B1 induces oxidative stress in oesophageal (SNO) cancer cells","volume":"141","author":"Khan","year":"2018","journal-title":"Toxic."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/s13567-015-0234-8","article-title":"Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis","volume":"46","author":"Antonissen","year":"2015","journal-title":"Vet. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Porras, N., Rebollada-Merino, A., B\u00e1rcena, C., Mayoral-Alegre, F.J., Lomillos, J.M., Dom\u00ednguez, L., and Rodr\u00edguez-Bertos, A. (2021). Effect of Biscuit Flour and Fermented Defatted \u201cAlperujo\u201d Co-Administration on Intestinal Mucosa Morphology and Productive Performance in Laying Hens. Animal, 11.","DOI":"10.3390\/ani11041075"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Huo, W., Qiao, Y., He, X., Wang, C., Li, R., Che, L., and Li, E. (2025). Mycotoxins and the Intestinal Epithelium: From Barrier Injury to Stem Cell Dysfunction. Toxin, 17.","DOI":"10.3390\/toxins17110534"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"9579188","DOI":"10.1155\/vmi\/9579188","article-title":"Efficacy of a Commercial Mycotoxin Adsorbent Against Low-Level Aflatoxin B1 and Fumonisin B1 Exposure in Broiler Chickens: In Vitro and In Vivo Assays","volume":"2026","author":"Luna","year":"2026","journal-title":"Vet. Med. Int."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sinclair-Black, M., Garcia, R.A., and Ellestad, L.E. (2023). Physiological regulation of calcium and phosphorus utilization in laying hens. Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1112499"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Huang, Z., Wang, G., Xu, M., Shi, Y., Feng, J., Zhang, M., and Li, C. (2025). Puerarin Enhances Eggshell Quality by Mitigating Uterine Senescence in Late-Phase Laying Breeder Hens. Antioxidant, 14.","DOI":"10.3390\/antiox14080960"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"136031","DOI":"10.1016\/j.foodchem.2023.136031","article-title":"Quantitative proteomics provides insights into the mechanism of the differences in heat-induced gel properties for egg white proteins with different interior quality during ageing in laying hens","volume":"419","author":"Li","year":"2023","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"103618","DOI":"10.1016\/j.psj.2024.103618","article-title":"Histological and molecular difference in albumen quality between post-adolescent hens and aged hens","volume":"103","author":"Chang","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Obianwuna, U.E., Oleforuh-Okoleh, V.U., Wang, J., Zhang, H.-J., Qi, G.-H., Qiu, K., and Wu, S.-G. (2022). Potential Implications of Natural Antioxidants of Plant Origin on Oxidative Stability of Chicken Albumen during Storage: A Review. Antioxidant, 11.","DOI":"10.3390\/antiox11040630"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2613\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:25:24Z","timestamp":1787243124000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/16\/2613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,20]]},"references-count":47,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ani16162613"],"URL":"https:\/\/doi.org\/10.3390\/ani16162613","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,20]]}}}