{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:35:29Z","timestamp":1787567729635,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biological Database"},{"name":"Convergent Animal + Human LLC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Conservation of native species is an ongoing task, especially when non-native species present a threat via competition and disease transmission. In Texas, native desert bighorn sheep (DBS) compete directly with non-native aoudad for resources. Aoudad (Ammotragus lervia, also known as Barbary sheep), introduced into the United States from Africa, are competent hosts of Mesomycoplasma ovipneumoniae and are thought to be the primary reservoir for transmitting pneumonia to DBS. A recent epidemic (circa December 2025) of infectious keratoconjunctivitis (IKC) in aoudad was reported in western Texas. Herein, we compare newly derived microbial data (16S rRNA sequencing) to results derived from a previous dataset (multi-locus sequence typing). Five M. conjunctivae haplotypes were indicated by 16S sequence data obtained from 33 individuals. Phylogenetic analyses identified these haplotypes as members of a clade containing sequences from wild ungulate hosts, whereas sequences from domestic ungulates formed a divergent clade (6.58%). Together, these data indicate the presence of an aoudad-like M. conjunctivae obtained from four mountain ranges in western Texas during 2018\u20132020. These implications suggest that monitoring for IKC and identifying the bacterial pathogen is paramount in case of spillover from aoudad to DBS. Based on the data presented herein, effective management strategies should take a dual approach of bacterial surveillance and preparation in case of antibiotic intervention for DBS conservation.<\/jats:p>","DOI":"10.3390\/ani16172639","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:20:02Z","timestamp":1787563202000},"page":"2639","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Independent Discovery of Mesomycoplasma conjunctivae in Non-Native Aoudad (Ammotragus lervia) in Texas"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0537-0567","authenticated-orcid":false,"given":"Emily A.","family":"Wright","sequence":"first","affiliation":[{"name":"Independent Researcher, Rockwall, TX 75032, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan R.","family":"Amman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Texas Tech University, 2901 Main St., Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Indiana","family":"Beck","sequence":"additional","affiliation":[{"name":"Independent Researcher, Needham, MA 02494, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caleb D.","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Texas Tech University, 2901 Main St., Lubbock, TX 79409, USA"},{"name":"Natural Science Research Laboratory, Museum at Texas Tech University, 3301 4th St., Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Texas Tech University, 2901 Main St., Lubbock, TX 79409, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"ref_1","unstructured":"Halls, L.K. (1984). Of Slings and Arrows: An Historical Retrospection. White-Tailed Deer: Ecology and Management, Stackpole Books."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e22523","DOI":"10.1002\/jwmg.22523","article-title":"Contrasting Management Paradigms for Pronghorn in the Arid Southwest and Their Northern Range: A Review","volume":"88","author":"Bean","year":"2024","journal-title":"J. Wildl. Manag."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6397","DOI":"10.1038\/s41598-022-09828-z","article-title":"Genomic Evaluation of Hybridization in Historic and Modern North American Bison (Bison bison)","volume":"12","author":"Stroupe","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Whiting, J.C., Bleich, V.C., Bowyer, R.T., and Epps, C.W. (2023). Restoration of Bighorn Sheep: History, Successes, and Remaining Conservation Issues. Front. Ecol. Evol., 11.","DOI":"10.3389\/fevo.2023.1083350"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"440","DOI":"10.2307\/1374591","article-title":"The Bighorn Sheep of Texas","volume":"20","author":"Davis","year":"1939","journal-title":"J. Mammal."},{"key":"ref_6","unstructured":"Cook, R.L. (1992). A Historical Review of Reports, Field Notes, and Correspondence on the Desert Bighorn Sheep in Texas: Special Report to the Desert Bighorn Sheep Advisory Committee: Contribution of Federal Aid Project W-127-R and W-123-D."},{"key":"ref_7","first-page":"102","article-title":"Status of Desert Bighorn Sheep in Texas\u20141982","volume":"26","author":"Kilpatric","year":"1982","journal-title":"Desert Bighorn Counc. Trans."},{"key":"ref_8","unstructured":"\u0110uri\u0107, M. (2022). Orbivirus caerulinguae (Bluetongue Virus), CABI Compendium."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e8849","DOI":"10.1002\/ece3.8849","article-title":"Distinct MtDNA Lineages in Free-ranging Ammotragus (Aoudad) from the United States Indicate Multiple Introductions from Northern Africa","volume":"12","author":"Wright","year":"2022","journal-title":"Ecol. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1093\/jmammal\/gyae078","article-title":"Translocations, Rising Populations, and Phylogeographic Consequences: Genomic Implications for Conservation of Introduced Aoudad (Ammotragus lervia) in the Southwestern United States","volume":"105","author":"Wright","year":"2024","journal-title":"J. Mammal."},{"key":"ref_11","unstructured":"Wright, E.A., and Bradley, R.D. (2023). Genetic Divergence and MtDNA Lineages in Six Subspecies of Aoudad, Museum of Texas Tech University."},{"key":"ref_12","unstructured":"Weidmeier, R.C. (2021). Characterization of Aoudad and Desert Bighorn Sheep Microbiomes in Association to Disease Risk, Texas Tech University."},{"key":"ref_13","unstructured":"Wilcox, D.C. (2022). Interactions between Aoudad, Desert Bighorn Sheep, and Mule Deer in the Texas Trans-Pecos, Sul Ross State University."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e22594","DOI":"10.1002\/jwmg.22594","article-title":"Multi-locus Sequence Typing Indicates Multiple Strains of Mycoplasma in Desert Bighorn Sheep and Aoudad in Texas","volume":"88","author":"Wright","year":"2024","journal-title":"J. Wildl. Manag."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21041","DOI":"10.1038\/s41598-026-51837-9","article-title":"Aoudad (Ammotragus lervia) as Disease Reservoirs for Bighorn Sheep (Ovis canadensis): Experimental and Epidemiological Evidence","volume":"16","author":"Thomas","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_16","unstructured":"Traweek, M., and Welch, R. (1992). Exotics in Texas."},{"key":"ref_17","first-page":"12","article-title":"Back from the Brink-Again? Texas Landowners and Wildlife Experts Confront Latest Bighorn Crisis","volume":"41","year":"2025","journal-title":"Tex. Wildl."},{"key":"ref_18","unstructured":"Neha Sufia, A., Wright, E.A., Brugette, G.G., Buckert, K.F., Phillips, C.D., and Bradley, R.D. (2026). Metagenomic Analysis of Diets in Sympatric Aoudad, Desert Bighorn Sheep, and Mule Deer in the Chihuahuan Desert. Ecol. Evol., in revision."},{"key":"ref_19","unstructured":"Texas Parks and Wildlife Department (2026). Status Update."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"407","DOI":"10.7589\/0090-3558-42.2.407","article-title":"Infectious Keratoconjunctivitis in Bighorn Sheep, Silver Bell Mountains, Arizona, USA","volume":"42","author":"Jansen","year":"2006","journal-title":"J. Wildl. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"571","DOI":"10.2193\/2005-617","article-title":"Population Dynamics and Behavior of Bighorn Sheep with Infectious Keratoconjunctivitis","volume":"71","author":"Jansen","year":"2007","journal-title":"J. Wildl. Manag."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"489","DOI":"10.7589\/2019-04-103","article-title":"Mycoplasma Conjunctivae-Associated Keratoconjunctivitis in Norwegian Muskox (Ovibos moschatus)","volume":"56","author":"Handeland","year":"2020","journal-title":"J. Wildl. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Thomas, L.F., Clontz, D., Nunez, C.M., Dittmar, R.O., Hernandez, F., Rech, R.R., and Cook, W.E. (2024). Evaluating the Transmission Dynamics and Host Competency of Aoudad (Ammotragus lervia) Experimentally Infected with Mycoplasma Ovipneumoniae and Leukotoxigenic Pasteurellaceae. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0294853"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e00075","DOI":"10.1097\/JU9.0000000000000082","article-title":"Microbiomes in Post\u2013Digital Rectal Exam Urine Samples Are Linked to Prostate Cancer Risk","volume":"1","author":"Crawford","year":"2023","journal-title":"JU Open Plus"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative Genomics Viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e01371-20","DOI":"10.1128\/MRA.01371-20","article-title":"Draft Genome Sequence of a Novel Mycoplasma Species Identified from the Respiratory Tract of an Alaska Moose (Alces alces gigas)","volume":"10","author":"Herndon","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_27","unstructured":"Swofford, D.L. (2003). PAUP*: Phylogenetic Analysis Using Parsimony (* and Other Methods), Sinauer Associates. Version 4; [Computer Program]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence Limits on Phylogenies: An Approach Using the Bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1093\/molbev\/msx248","article-title":"DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets","volume":"34","author":"Rozas","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"POPART: Full-Feature Software for Haplotype Network Construction","volume":"6","author":"Leigh","year":"2015","journal-title":"Methods Ecol. Evol."},{"key":"ref_32","first-page":"184","article-title":"TCS: Estimating Gene Genealogies","volume":"Volume 3","author":"Clement","year":"2002","journal-title":"Proceedings of the 16th International Parallel and Distributed Processing Symposium"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"15318","DOI":"10.1038\/s41598-019-51444-x","article-title":"Genetic Structure of Mycoplasma Ovipneumoniae Informs Pathogen Spillover Dynamics between Domestic and Wild Caprinae in the Western United States","volume":"9","author":"Kamath","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.prevetmed.2012.11.018","article-title":"Bighorn Sheep Pneumonia: Sorting out the Cause of a Polymicrobial Disease","volume":"108","author":"Besser","year":"2013","journal-title":"Prev. Vet. Med."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Thomas, L.F., Panaretos, C., Scott, M.A., Valeris-Chacin, R., and Cook, W.E. (2026). Distinct Host\u2013Pathogen and Microbiome Responses of Aoudad (Ammotragus lervia) and Bighorn Sheep (Ovis canadensis) Following Exposure to Mycoplasma ovipneumoniae with or without Co-Exposure to Leukotoxigenic Pasteurellaceae. BMC Vet. Res., 22.","DOI":"10.1186\/s12917-026-05375-1"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2639\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:38:15Z","timestamp":1787564295000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,23]]},"references-count":35,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16172639"],"URL":"https:\/\/doi.org\/10.3390\/ani16172639","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,23]]}}}