{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:29:58Z","timestamp":1787646598624,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated the effects of partially (50%) or completely (100%) replacing soybean oil with Hermetia illucens larvae fat (HI fat) in diets for laying hens on productive performance, egg quality, yolk lipid composition, and ovarian follicle characteristics during a 20-week feeding period. Sixty Lohmann LSL Classic hens were randomly allocated to three dietary treatments (Control, HI50, and HI100; n = 20 hens per treatment). Due to space limitations within the experimental facility, each treatment was housed in a single pen, which precluded statistical analysis of production performance data. Nevertheless, descriptive evaluation revealed no obvious differences in laying rate, egg weight, egg mass, feed intake, or feed conversion ratio among the dietary treatments. Most egg quality traits, including albumen height, Haugh units, shell strength, and yolk color, remained unaffected, although minor transient differences were detected at individual sampling time points. Dietary treatments altered yolk fatty acid composition, reflecting the fatty acid profile of the experimental diets. Replacement of soybean oil with HI fat increased the proportions of saturated fatty acids, particularly lauric (C12:0), myristic (C14:0), and palmitic acids (C16:0), while reducing both n-6 and n-3 polyunsaturated fatty acids. In conclusion, the findings of this exploratory study indicate that HI fat may have potential as an alternative lipid source in layer diets. Under the conditions of the present experiment, no obvious adverse effects on productive performance, egg quality, or ovarian follicle characteristics were observed. However, because each dietary treatment was represented by a single pen, the results should be interpreted with caution and require confirmation in studies with replicated experimental units.<\/jats:p>","DOI":"10.3390\/ani16172661","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:00:48Z","timestamp":1787644848000},"page":"2661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Replacement of Soybean Oil with Hermetia illucens Larvae Fat in Layer Diets: Effects on Performance, Egg Quality, Yolk Lipid Composition, and Ovarian Follicles"],"prefix":"10.3390","volume":"16","author":[{"given":"Franziska","family":"Hornfeck","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronny","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Gefl\u00fcgelhof Thierbach GmbH, Thierbach 45, 07368 Remptendorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2958-2422","authenticated-orcid":false,"given":"Sarah M.","family":"Grundmann","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2954-4960","authenticated-orcid":false,"given":"Kristina","family":"Maier-Sam","sequence":"additional","affiliation":[{"name":"Clinic for Birds, Reptiles, Amphibians and Fish, Justus Liebig University Giessen, Frankfurter Strasse 114, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Most","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7934-1304","authenticated-orcid":false,"given":"Denise K.","family":"Gessner","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3246-4136","authenticated-orcid":false,"given":"Klaus","family":"Eder","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany"},{"name":"Center for Sustainable Food Systems, Justus Liebig University Giessen, Senkenbergstrasse 3, 35390 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Herrero, M., Mason-D\u2019Croz, D., Thornton, P.K., Fanzo, J., Rushton, J., Godde, C., Bellows, A., de Groot, A., Palmer, J., and Chang, J. (2023). Science and Innovations for Food Systems Transformation: Livestock and Sustainable Food Systems: Status, Trends, and Priority Actions, Springer.","DOI":"10.1007\/978-3-031-15703-5_20"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106617","DOI":"10.1016\/j.psj.2026.106617","article-title":"Strategic role of poultry production sciences in shaping the future of global food security and strengthen sustainability","volume":"105","author":"Nassar","year":"2026","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"104295","DOI":"10.1016\/j.psj.2024.104295","article-title":"Sustainable poultry farming practices: A critical review of current strategies and future prospects","volume":"103","author":"Bist","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0043933917000071","article-title":"Global poultry production: Current state and future outlook and challenges","volume":"73","author":"Mottet","year":"2017","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_5","unstructured":"FAOSTAT (2025, December 07). Crops and Livestock Products. Available online: https:\/\/www.fao.org\/faostat\/en\/#data\/QCL."},{"key":"ref_6","unstructured":"Veldkamp, T., van Duinkerken, G., van Huis, A., Lakemond, C.M.M., Ottevanger, E., Bosch, G., and van Boekel, M.A.J.S. (2012). Insects as a Sustainable Feed Ingredient in Pig and Poultry Diets: A Feasibility Study, Wageningen UR Livestock Research."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102067","DOI":"10.1016\/j.gloenvcha.2020.102067","article-title":"Spatially-explicit footprints of agricultural commodities: Mapping carbon emissions embodied in Brazil\u2019s soy exports","volume":"62","author":"Escobar","year":"2020","journal-title":"Glob. Environ. Change"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Boakye-Yiadom, K.A., Ilari, A., and Duca, D. (2022). Greenhouse Gas Emissions and Life Cycle Assessment on the Black Soldier Fly (Hermetia illucens L.). Sustainability, 14.","DOI":"10.3390\/su141610456"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Khalifah, A., Abdalla, S., Rageb, M., Maruccio, L., Ciani, F., and El-Sabrout, K. (2023). Could Insect Products Provide a Safe and Sustainable Feed Alternative for the Poultry Industry? A Comprehensive Review. Animals, 13.","DOI":"10.3390\/ani13091534"},{"key":"ref_10","unstructured":"European Commission (2021). Commission Regulation (EU) 2021\/1372 of 17 August 2021 ammending Annex IV to Regulation (EC) 999\/2001 of the European Parliament and of the Council as regards the prohition to feed non-ruminant farmed animals, other than fur animals, with protein derived from animals. Off. J. Eur. Union, L295, 1\u201317. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2021\/1372\/oj."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s40104-018-0266-9","article-title":"Black soldier fly defatted meal as a dietary protein source for broiler chickens: Effects on growth performance, blood traits, gut morphology and histological features","volume":"9","author":"Dabbou","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"103635","DOI":"10.1016\/j.psj.2024.103635","article-title":"Effect of replacing soybean meal with Hermetia illucens meal on cecal microbiota, liver transcriptome, and plasma metabolome of broilers","volume":"103","author":"Beller","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2032","DOI":"10.1017\/S1751731117003743","article-title":"Black soldier fly larva fat inclusion in finisher broiler chicken diet as an alternative fat source","volume":"12","author":"Schiavone","year":"2018","journal-title":"Animal"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s40104-017-0181-5","article-title":"Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens: Apparent nutrient digestibility, apparent metabolizable energy and apparent ileal amino acid digestibility","volume":"8","author":"Schiavone","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1163\/23524588-20230106","article-title":"Apparent nutrient digestibility, metabolizable energy and apparent ileal amino acid digestibility of commercial partially defatted Hermetia illucens meal for laying hens","volume":"10","author":"Kwakernaak","year":"2023","journal-title":"J. Insects Food Feed."},{"key":"ref_16","first-page":"441","article-title":"Effect of Black Soldier Fly Larva Meal on Broiler Chicken Production, Meat Quality, and It\u2019s Physiological Properties: A Meta-Analysis","volume":"31","author":"Chhetri","year":"2025","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1080\/1745039X.2023.2190297","article-title":"Replacement of soybean oil by Hermetia illucens larvae fat in broiler diets alters the breast muscle lipidome and reduces lipid oxidation of the breast muscle during heat-processing","volume":"77","author":"Grundmann","year":"2023","journal-title":"Arch. Anim. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1472-765X.2005.01709.x","article-title":"Susceptibility of Clostridium perfringens to C-C fatty acids","volume":"41","author":"Marounek","year":"2005","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1002\/jsfa.8081","article-title":"Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates","volume":"97","author":"Spranghers","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"101315","DOI":"10.1016\/j.psj.2021.101315","article-title":"Serum metabolome and gut microbiome alterations in broiler chickens supplemented with lauric acid","volume":"100","author":"Wu","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"104460","DOI":"10.1016\/j.psj.2024.104460","article-title":"Effect of black soldier fly (Hermetia illucens) larvae meal and oil on the performance, biochemical profile, intestinal health and gut microbial dynamics in laying hens","volume":"103","author":"Khan","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Cullere, M., Schiavone, A., Dabbou, S., Gasco, L., and Dalle Zotte, A. (2019). Meat Quality and Sensory Traits of Finisher Broiler Chickens Fed with Black Soldier Fly (Hermetia illucens L.) Larvae Fat as Alternative Fat Source. Animals, 9.","DOI":"10.3390\/ani9040140"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/1828051X.2016.1249968","article-title":"Partial or total replacement of soybean oil by black soldier fly larvae (Hermetia illucens L.) fat in broiler diets: Effect on growth performances, feed-choice, blood traits, carcass characteristics and meat quality","volume":"16","author":"Schiavone","year":"2017","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40104-023-00831-6","article-title":"Effect of replacement of soybean oil by Hermetia illucens fat on performance, digestibility, cecal microbiome, liver transcriptome and liver and plasma lipidomes of broilers","volume":"14","author":"Grundmann","year":"2023","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"18086","DOI":"10.1038\/s41598-025-02820-3","article-title":"Insect fat influences broiler performance, meat quality, and the cecal microbiota similarly to plant oils rather than animal fats","volume":"15","author":"Aslam","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101272","DOI":"10.1016\/j.psj.2021.101272","article-title":"The impact of dietary Black Soldier Fly larvae oil and meal on laying hen performance and egg quality","volume":"100","author":"Patterson","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.5713\/ab.22.0052","article-title":"Black soldier fly (Hermetia illucens) larvae oil as an alternative fat ingredient to soybean oil in laying hen diets","volume":"35","author":"Kim","year":"2022","journal-title":"Anim. Biosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"101034","DOI":"10.1016\/j.psj.2021.101034","article-title":"Black soldier fly larvae meal and fat can completely replace soybean cake and oil in diets for laying hens","volume":"100","author":"Heuel","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_29","first-page":"641","article-title":"The potential of probiotic (Bacillus subtilis and Bacillus licheniformis) and Black Soldier Fly Larvae combination as a supplement for late-phase layer hens: Antimicrobial and enzyme activities","volume":"14","author":"Kumalasari","year":"2025","journal-title":"Int. J. Vet. Sci."},{"key":"ref_30","first-page":"227","article-title":"Production performance and egg quality of laying hens fed with diet containing black soldier fly (Hermetia illucens) larvae: A meta-analysis","volume":"14","author":"Wahyuni","year":"2025","journal-title":"Int. J. Vet. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Izuddin, W.I., Loh, T.C., Nayan, N., Akit, H., Noor, A.M., and Foo, H.L. (2023). Blood lipid profiles, fatty acid deposition and expression of hepatic lipid and lipoprotein metabolism genes in laying hens fed palm oils, palm kernel oil, and soybean oil. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1192841"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1080\/00439339.2023.2189206","article-title":"Lipid metabolism and body composition in long-term producing hens","volume":"79","author":"Enting","year":"2023","journal-title":"World\u2019s Poult. Sci. J."},{"key":"ref_33","unstructured":"Lohmann Breeders (2022). LOHMANN LSL-Classic Layers Management Guide, Lohmann Breeders GmbH."},{"key":"ref_34","unstructured":"Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten (2012). Das Handbuch der Landwirtschaftlichen Versuchs- und Untersuchungsmethodik: Methodenbuch Band III Futtermittel, VDLUFA Verlag."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6837","DOI":"10.1016\/j.psj.2020.08.082","article-title":"Effects of supplementation of DL-methionine on tissue and plasma antioxidant status during heat-induced oxidative stress in broilers","volume":"99","author":"Zeitz","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_36","unstructured":"Association of Official Analytical Chemists (1995). Official Methods of Analysis, Association of Analytical Communities. [16th ed.]."},{"key":"ref_37","unstructured":"World\u2019s Poultry Science Association (1984). The prediction of apparent metabolizable energy values for poultry in compound feeds. World\u2019s Poult. Sci. J., 40, 181\u2013182."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1093\/jn\/124.10.1917","article-title":"Dietary fat influences the effect of zinc deficiency on liver lipids and fatty acids in rats force-fed equal quantities of diet","volume":"124","author":"Eder","year":"1994","journal-title":"J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.2527\/jas.2010-3617","article-title":"Influence of a rumen-protected conjugated linoleic acid mixture on carcass traits and meat quality in young Simmental heifers","volume":"90","author":"Schlegel","year":"2012","journal-title":"J. Anim. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.tjnut.2023.09.019","article-title":"Fat from Hermetia illucens Alters the Cecal Gut Microbiome and Lowers Hepatic Triglyceride Concentration in Comparison to Palm Oil in Obese Zucker Rats","volume":"154","author":"Marschall","year":"2024","journal-title":"J. Nutr."},{"key":"ref_41","first-page":"133","article-title":"Effect of Peppermint Oil (Mentha piperita) Supplementation in Quail Diets on Egg Production and Some Egg Quality Characteristics, Oxidative Stress Parameters and Lipid Profile","volume":"31","author":"Evci","year":"2025","journal-title":"Kafkas Univ. Vet. Fak. Derg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"101986","DOI":"10.1016\/j.psj.2022.101986","article-title":"Egg quality and laying performance of Julia laying hens fed with black soldier fly (Hermetia illucens) larvae meal as a long-term substitute for fish meal","volume":"101","author":"Zhao","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"104229","DOI":"10.1016\/j.psj.2024.104229","article-title":"Performance, egg quality and organ traits of laying hens fed black soldier fly larvae products","volume":"103","author":"Gort","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Secci, G., Bovera, F., Parisi, G., and Moniello, G. (2020). Quality of Eggs and Albumen Technological Properties as Affected by Hermetia illucens Larvae Meal in Hens\u2019 Diet and Hen Age. Animals, 10.","DOI":"10.3390\/ani10010081"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.2478\/aoas-2020-0076","article-title":"Insect Fat in Animal Nutrition\u2014A Review","volume":"20","author":"Benzertiha","year":"2020","journal-title":"Ann. Anim. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0003-9098(25)00687-3","article-title":"Laying performance and fatty acid composition of egg yolk lipids of hens fed diets with various amounts of ground or whole flaxseed","volume":"62","author":"Eder","year":"1998","journal-title":"Eur. Poult. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1016\/j.psj.2019.11.005","article-title":"Comparative effects of dietary microalgae oil and fish oil on fatty acid composition and sensory quality of table eggs","volume":"99","author":"Feng","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1111\/jpn.13364","article-title":"Effects of feeding extruded flaxseed on layer performance, total tract nutrient digestibility, and fatty acid concentrations of egg yolk, plasma and liver","volume":"104","author":"Huang","year":"2020","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1002\/lipd.12304","article-title":"Transfer of Lauric and Myristic Acid from Black Soldier Fly Larval Lipids to Egg Yolk Lipids of Hens Is Low","volume":"56","author":"Heuel","year":"2021","journal-title":"Lipids"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1748-1716.1965.tb04194.x","article-title":"The metabolism of fatty acids in the rat. 8. Lauric acid and myristic acid","volume":"64","year":"1965","journal-title":"Acta Physiol. Scand."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1093\/ajcn\/72.4.905","article-title":"Differential oxidation of individual dietary fatty acids in humans","volume":"72","author":"DeLany","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Romero, C., Cenalmor, J.C., Chamorro, S., and Redondo, C. (2024). Effect of Different Dietary Doses of Black Soldier Fly Meal on Performance and Egg Quality in Free-Range Reared Laying Hens. Animals, 14.","DOI":"10.3390\/ani14223340"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"805S","DOI":"10.1093\/jn\/127.5.805S","article-title":"Lipoprotein metabolism and fattening in poultry","volume":"127","author":"Hermier","year":"1997","journal-title":"J. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3121","DOI":"10.1016\/j.psj.2020.01.017","article-title":"Dietary betaine improves egg-laying rate in hens through hypomethylation and glucocorticoid receptor\u2013mediated activation of hepatic lipogenesis-related genes","volume":"99","author":"Omer","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.3382\/ps\/pev191","article-title":"Effects of dietary fats rich in lauric and myristic acid on performance, intestinal morphology, gut microbes, and meat quality in broilers","volume":"94","author":"Zeitz","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1136\/bmj.314.7074.112","article-title":"Dietary lipids and blood cholesterol: Quantitative meta-analysis of metabolic ward studies","volume":"314","author":"Clarke","year":"1997","journal-title":"BMJ"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1093\/jn\/nxy156","article-title":"Corn Oil Lowers Plasma Cholesterol Compared with Coconut Oil in Adults with Above-Desirable Levels of Cholesterol in a Randomized Crossover Trial","volume":"148","author":"Maki","year":"2018","journal-title":"J. Nutr."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1017\/S0029665120007077","article-title":"n-3 PUFA and inflammation: From membrane to nucleus and from bench to bedside","volume":"79","author":"Calder","year":"2020","journal-title":"Proc. Nutr. Soc."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1210\/clinem\/dgae445","article-title":"Omega-3 Polyunsaturated Fatty Acids and Adipose Tissue Inflammation: Longitudinal Analysis in the PROMISE Cohort","volume":"110","author":"Chon","year":"2025","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Liput, K.P., Lepczy\u0144ski, A., Og\u0142uszka, M., Nawrocka, A., Po\u0142awska, E., Grzesiak, A., \u015alaska, B., Pareek, C.S., Czarnik, U., and Pierzcha\u0142a, M. (2021). Effects of Dietary n-3 and n-6 Polyunsaturated Fatty Acids in Inflammation and Cancerogenesis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22136965"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3382\/ps.0690288","article-title":"Follicular control of oviposition in the hen","volume":"69","author":"Kelly","year":"1990","journal-title":"Poult. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"102497","DOI":"10.1016\/j.psj.2023.102497","article-title":"Effect of dietary concentrations of metabolizable energy and neutral detergent fiber on productive performance, egg quality, fatty liver incidence, and hepatic fatty acid metabolism in aged laying hens","volume":"102","author":"Han","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Zhao, J., Pan, H., Liu, Y., He, Y., Shi, H., and Ge, C. (2023). Interacting Networks of the Hypothalamic-Pituitary-Ovarian Axis Regulate Layer Hens Performance. 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