{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:23:48Z","timestamp":1788337428743,"version":"build-2803163510"},"reference-count":152,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Foundation for Advanced Talents of InnerMongolia Normal University","award":["2025YJRC077"],"award-info":[{"award-number":["2025YJRC077"]}]},{"name":"Inner Mongolia Natural Science Foundation Youth Fund","award":["2026QC0631"],"award-info":[{"award-number":["2026QC0631"]}]},{"name":"Development and Utilization of Raw and Auxiliary Materials for Biopharmaceuticals","award":["2025HX016"],"award-info":[{"award-number":["2025HX016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Yaks are indigenous livestock species of the Qinghai\u2013Tibet Plateau. Due to long-term exposure to extreme environmental stressors, including high altitude, hypoxia, and low temperatures, its male reproductive system has evolved distinct adaptive strategies. Specifically, testicular weight is markedly lower than that of cattle. Nevertheless, according to reported data from different experiments, although its single ejaculate volume is lower than that of Tibetan cattle, it can still maintain comparable fresh sperm motility. These reproductive phenotypes make the yak an ideal model organism for investigating plateau adaptation and sperm energy metabolism. The present review synthesizes continuous molecular events in yak testes spanning embryonic development through senescence. It focuses on dissecting the core regulatory networks governing spermatogonial stem cell self-renewal, meiosis, and spermiogenesis. It integrates the latest advances in testicular microenvironment dynamics and epigenetic modifications. Additionally, male sterility in cattle-yak serves as a natural mutant model. Essential regulatory modules governing spermatogenesis can be inferred from its spermatogenic-arrest phenotype. Nevertheless, comparative omics analyses between yaks and cattle are confounded by seasonal variation and differing genetic backgrounds. It is therefore critical to distinguish signatures driven by genetic adaptation from those arising from environmental plasticity. Finally, this paper presents research prospects regarding how to utilize single-cell multi-omics and gene-editing technologies to deeply dissect the underlying mechanisms of plateau reproductive adaptation to provide theoretical support for improving yak fecundity and hybrid breeding.<\/jats:p>","DOI":"10.3390\/ani16172730","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T07:53:11Z","timestamp":1788335591000},"page":"2730","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advances in the Molecular Regulatory Mechanisms of Testicular Development and Spermatogenesis in Yaks"],"prefix":"10.3390","volume":"16","author":[{"given":"Qiqi","family":"Yin","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China"},{"name":"Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxing","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China"},{"name":"Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6008-4731","authenticated-orcid":false,"given":"Xingdong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China"},{"name":"Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongming","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China"},{"name":"Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/s41586-022-05547-7","article-title":"The molecular evolution of spermatogenesis across mammals","volume":"613","author":"Murat","year":"2023","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.aninu.2022.02.002","article-title":"The adaptive strategies of yaks to live in the Asian highlands","volume":"9","author":"Jing","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12041-019-1091-4","article-title":"Identification and expression profiling of MSY genes of yak for bull fertility","volume":"98","author":"Das","year":"2019","journal-title":"J. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s40104-019-0360-7","article-title":"Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak","volume":"10","author":"Wang","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Hu, L., Wang, X., Guo, S., Cao, M., Kang, Y., Ding, Z., Pei, J., Ge, Q., Ma, Y., and Guo, X. (2024). Whole-transcriptome sequencing analysis to identify key circRNAs, miRNAs, and mRNAs in the development of yak testes. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10716-1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1002\/(SICI)1098-2353(2000)13:2<146::AID-CA10>3.0.CO;2-K","article-title":"Human Embryology and Developmental Biology","volume":"13","author":"Gilroy","year":"2000","journal-title":"Clin. Anat."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1101\/gad.228080.113","article-title":"Building the mammalian testis: Origins, differentiation, and assembly of the component cell populations","volume":"27","author":"Svingen","year":"2013","journal-title":"Genes Dev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1530\/JME-17-0314","article-title":"The molecular pathways underlying early gonadal development","volume":"62","author":"Yang","year":"2019","journal-title":"J. Mol. Endocrinol."},{"key":"ref_9","first-page":"4","article-title":"Early development of the human embryonic testis","volume":"129","author":"Takahashi","year":"2022","journal-title":"Differentiation"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1530\/rep.0.1250769","article-title":"Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood","volume":"125","author":"Sharpe","year":"2003","journal-title":"Reproduction"},{"key":"ref_11","first-page":"335","article-title":"Changes in Histomorphology and Reproductive Hormone Levels during Testicular Development in Guangxi Bama Miniature Pigs","volume":"60","author":"Zhang","year":"2024","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1111\/j.1742-4658.2011.08029.x","article-title":"Sex determination and the control of Sox9 expression in mammals","volume":"278","author":"Jakob","year":"2011","journal-title":"FEBS J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1038\/nature06944","article-title":"Sex determination involves synergistic action of SRY and SF1 on a specific Sox9 enhancer","volume":"453","author":"Sekido","year":"2008","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1159\/000252791","article-title":"Profiles of Gonadal Gene Expression in the Developing Bovine Embryo","volume":"3","author":"Ross","year":"2009","journal-title":"Sex. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1677\/joe.1.06879","article-title":"Expression and functional role of hepatocyte growth factor and its receptor (c-met) during fetal mouse testis development","volume":"191","author":"Ricci","year":"2006","journal-title":"J. Endocrinol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1038\/s41467-025-59183-6","article-title":"NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development","volume":"16","author":"Estermann","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Gao, Y., Wang, Z., Long, Y., Yang, L., Jiang, Y., Ding, D., Teng, B., Chen, M., Yuan, J., and Gao, F. (2024). Unveiling the roles of Sertoli cells lineage differentiation in reproductive development and disorders: A review. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1357594"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e2419995122","DOI":"10.1073\/pnas.2419995122","article-title":"Testicular somatic and germ cell maturation during rhesus macaque development","volume":"122","author":"Sosa","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","first-page":"1009","article-title":"CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells","volume":"126","author":"Yang","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4011","DOI":"10.1242\/dev.041160","article-title":"Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells","volume":"136","author":"Yamaguchi","year":"2009","journal-title":"Development"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1247\/csf.26.131","article-title":"Vasa homolog genes in mammalian germ cell development","volume":"26","author":"Noce","year":"2001","journal-title":"Cell Struct. Funct."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1095\/biolreprod.109.078717","article-title":"Critical function of AP-2 gamma\/TCFAP2C in mouse embryonic germ cell maintenance","volume":"82","author":"Weber","year":"2010","journal-title":"Biol. Reprod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"28314","DOI":"10.1016\/S0021-9258(18)46930-X","article-title":"The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR)","volume":"269","author":"Clark","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1172\/JCI25091","article-title":"In humans, early cortisol biosynthesis provides a mechanism to safeguard female sexual development","volume":"116","author":"Goto","year":"2006","journal-title":"J. Clin. Investig."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e2501392122","DOI":"10.1073\/pnas.2501392122","article-title":"Single-cell resolution uncovers neighboring cell subtypes that share steroidogenic capacity during fetal testis development","volume":"122","author":"Jiang","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.molcel.2008.09.003","article-title":"A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice","volume":"31","author":"Aravin","year":"2008","journal-title":"Mol. Cell"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.devcel.2007.03.001","article-title":"MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline","volume":"12","author":"Carmell","year":"2007","journal-title":"Dev. Cell"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"La, Y., Ma, X., Bao, P., Chu, M., Yan, P., Guo, X., and Liang, C. (2022). Identification and Characterization of Piwi-Interacting RNAs for Early Testicular Development in Yak. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012320"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1002\/dvdy.669","article-title":"Loss of function of male-specific lethal 3 (Msl3) does not affect spermatogenesis in rodents","volume":"253","author":"Mitchell","year":"2023","journal-title":"Dev. Dyn."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/s42003-025-09467-w","article-title":"Integrated multiomics analysis reveals the molecular features and crucial regulators of hair follicles in yak (Bos grunniens)","volume":"9","author":"Zhang","year":"2026","journal-title":"Commun. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Yan, Q., Wang, Q., Zhang, Y., Yuan, L., Hu, J., and Zhao, X. (2024). The Novel-m0230-3p miRNA Modulates the CSF1\/CSF1R\/Ras Pathway to Regulate the Cell Tight Junctions and Blood-Testis Barrier in Yak. Cells, 13.","DOI":"10.3390\/cells13151304"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"La, Y., Ma, X., Bao, P., Chu, M., Yan, P., Guo, X., and Liang, C. (2024). Quantitative Proteomic Analysis Reveals Key Proteins Involved in Testicular Development of Yaks. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25158433"},{"key":"ref_33","unstructured":"Wang, X. (2024). Molecular Mechanism of Yak Testicular Development Based on Full-Length and Single-Cell Transcriptome Analysis. [Doctoral Dissertation, Chinese Academy of Agricultural Sciences]."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"La, Y., Ma, X., Bao, P., Chu, M., Yan, P., Liang, C., and Guo, X. (2023). Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054420"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Cao, M., Guo, S., Ding, Z., Hu, L., Xiong, L., Ge, Q., Pei, J., and Guo, X. (2025). Comparative Analysis of Testicular Transcriptional and Translational Landscapes in Yak and Cattle\u2013Yak: Implications for Hybrid Male Sterility. Biomolecules, 15.","DOI":"10.3390\/biom15081080"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Guo, S., Pei, J., Wang, X., Cao, M., Xiong, L., Kang, Y., Ding, Z., La, Y., Chu, M., and Bao, P. (2023). Transcriptome Studies Reveal the N6-Methyladenosine Differences in Testis of Yaks at Juvenile and Sexual Maturity Stages. Animals, 13.","DOI":"10.3390\/ani13182815"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/s12041-020-01248-0","article-title":"Characterizing miRNA and mse-tsRNA in fertile and subfertile yak bull spermatozoa from Arunachal Pradesh","volume":"99","author":"Das","year":"2020","journal-title":"J. Genet."},{"key":"ref_38","first-page":"116","article-title":"Gene Expression and Protein Localization of LYZL4 and LYZL6 in Testes of Different Months Old Yak","volume":"48","author":"Li","year":"2019","journal-title":"J. Henan Agric. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"23344213","DOI":"10.1080\/10495398.2024.2344213","article-title":"Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak","volume":"35","author":"La","year":"2024","journal-title":"Anim. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/rda.14345","article-title":"Comparison of MMP-2 and TIMP-2 expressions in yak testes at different ages","volume":"58","author":"Song","year":"2023","journal-title":"Reprod. Domest. Anim."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kalwar, Q., Ding, X., Ahmad, A.A., Chu, M., Wu, X., Bao, P., and Yan, P. (2019). Expression Analysis of IZUMO1 Gene during Testicular Development of Datong Yak (Bos grunniens). Animals, 9.","DOI":"10.3390\/ani9060292"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"La, Y., Ma, X., Bao, P., Chu, M., Guo, X., Liang, C., and Yan, P. (2023). Identification and profiling of microRNAs during yak\u2019s testicular development. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-023-03602-7"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1360\/N052013-00078","article-title":"Comparative transcriptome analysis of spermatogenesis arrest in cattle-yak","volume":"44","author":"Zeng","year":"2014","journal-title":"Sci. Sin. Vitae"},{"key":"ref_44","first-page":"229","article-title":"Cloning and expression of growth factor receptor bound protein 2 (GRB2) gene in yak (Bos grunniens)","volume":"46","author":"Lv","year":"2020","journal-title":"J. Southwest Minzu Univ."},{"key":"ref_45","first-page":"877","article-title":"Cloning of PGRMC1 Gene and Comparative Analysis of Its Expression in Tissues Related to Reproduction between Yak and Cattle","volume":"33","author":"Yang","year":"2018","journal-title":"J. Yunnan Agric. Univ."},{"key":"ref_46","first-page":"43","article-title":"Differential Analysis of Expressions of INH\u03b2B, FSHR and LHR during Development of Testes in Yaks","volume":"43","author":"Long","year":"2022","journal-title":"Prog. Vet. Med."},{"key":"ref_47","first-page":"72","article-title":"The Transcriptomic Landscape of Yaks Reveals Molecular Pathways for High Altitude Adaptation","volume":"11","author":"Qi","year":"2018","journal-title":"Genome Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/rda.14044","article-title":"Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak","volume":"57","author":"La","year":"2021","journal-title":"Reprod. Domest. Anim."},{"key":"ref_49","first-page":"310","article-title":"Cloning of Yak Cyclin D1 Gene and the Effects of IGF-I on ITs Expression in Sertoli Cells","volume":"49","author":"Wang","year":"2018","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Chen, M., Duan, C., Yin, X., Li, X., Liu, X., Zhang, L., Yue, S., Zhang, Y., and Liu, Y. (2023). Prolactin inhibitor changes testosterone production, testicular morphology, and related genes expression in cashmere goats. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1249189"},{"key":"ref_51","first-page":"9","article-title":"Mechanism of circadian clock gene regulating testosterone secretion in yak","volume":"51","author":"Gao","year":"2023","journal-title":"J. Northwest A F Univ. (Nat. Sci. Ed.)"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Li, T., Yan, Q., Nan, J., Huang, X., Wang, R., Zhang, Y., Zhao, X., and Wang, Q. (2024). LncRNA-MSTRG.19083.1 Targets NTRK2 as a miR-429-y Sponge to Regulate Circadian Rhythm via the cAMP Pathway in Yak Testis and Cryptorchidism. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252413553"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"msag027","DOI":"10.1093\/molbev\/msag027","article-title":"Dynamic regulation of spermatogenesis and hybrid sterility revealed by single-cell analysis in yak and cattle","volume":"43","author":"Wu","year":"2026","journal-title":"Mol. Biol. Evol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"909","DOI":"10.5187\/jast.2024.e52","article-title":"Brain-derived neurotrophic factor and neurotrophic tyrosine receptor kinase-2 in stallion testes: Insights into seasonal changes and potential roles in spermatogenesis","volume":"67","author":"Shakeel","year":"2025","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"37","DOI":"10.4103\/aja202457","article-title":"A behind-the-scenes role of BDNF in the survival and differentiation of spermatogonia","volume":"27","author":"Tomizawa","year":"2024","journal-title":"Asian J. Androl."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"eaee7124","DOI":"10.1126\/sciadv.aee7124","article-title":"Time-series single-cell transcriptomics reveals pervasive daily rhythmicity and nocturnal spermatogenesis in the zebrafish testis","volume":"12","author":"Liu","year":"2026","journal-title":"Sci. Adv."},{"key":"ref_57","first-page":"271","article-title":"Regulation of seasonal spermatogenesis in mammals","volume":"45","author":"Pan","year":"2025","journal-title":"Acta Theriol. Sin."},{"key":"ref_58","unstructured":"Ma, F.L. (2020). Expression and Localization of LHR\/PRLR and GH\/IGF-1 Genes in Testicles of Yak at Different Developmental Stages. [Master\u2019s Thesis, Northwest Minzu University]."},{"key":"ref_59","first-page":"672","article-title":"Expression and Localization of LHR and PRLR Genes in Testicles of Yak (Bos grunniens) at Different Developmental Stages","volume":"28","author":"Ma","year":"2020","journal-title":"J. Agric. Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Edelsztein, N.Y., and Rey, R.A. (2019). Importance of the Androgen Receptor Signaling in Gene Transactivation and Transrepression for Pubertal Maturation of the Testis. Cells, 8.","DOI":"10.20944\/preprints201907.0061.v1"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wang, J.-M., Li, Z.-F., and Yang, W.-X. (2022). What Does Androgen Receptor Signaling Pathway in Sertoli Cells During Normal Spermatogenesis Tell Us?. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.838858"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Yan, Q., Li, T., Zhang, Y., Zhao, X., Wang, Q., and Yuan, L. (2024). Caveolin 1 Regulates the Tight Junctions between Sertoli Cells and Promotes the Integrity of Blood-Testis Barrier in Yak via the FAK\/ERK Signaling Pathway. Animals, 14.","DOI":"10.3390\/ani14020183"},{"key":"ref_63","first-page":"207","article-title":"Analysis of Differential Expression of ET-1\/eNOS in the Development of Cryptorchidism in Yak","volume":"55","author":"Zhang","year":"2024","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_64","unstructured":"Yan, P., Guo, X., Liang, C.N., and Zhu, X.S. (2010, January 1\u20134). Reproductive Characteristics and Spermatogenesis in Yak. Proceedings of the 15th Academic Symposium of the Animal Reproduction Branch of the Chinese Association of Animal Science and Veterinary Medicine, Tianjin, China."},{"key":"ref_65","first-page":"855","article-title":"The Histologic Characters of Testis in Aging Yak","volume":"46","author":"Yuan","year":"2015","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_66","first-page":"2431","article-title":"Cloning of Yak SIRT3 Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages","volume":"50","author":"Wang","year":"2019","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"132400","DOI":"10.1016\/j.ijbiomac.2024.132400","article-title":"Complete characterization of the yak testicular development using accurate full-length transcriptome sequencing","volume":"271","author":"Wang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"146519","DOI":"10.1016\/j.ijbiomac.2025.146519","article-title":"Ribosome profiling sequencing reveals translational dynamics during yak testicular development","volume":"321","author":"Guo","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_69","unstructured":"Liu, P.G. (2018). HSPs Expression in Different Tissues and Their Effect on Testicular Development of Yak and Cattle-Yak. [Doctoral Dissertation, Gansu Agricultural University]."},{"key":"ref_70","unstructured":"Liu, M.Q. (2021). Yak (Bos grunniens) AQP1 and AQP11 Gene Cloning and Their Expression in the Testes at Different Developmental Stages. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_71","first-page":"933","article-title":"Cloning of Yak (Bos grunniens) AQP1 Gene and Its Expression in Yak Testis of Different Ages","volume":"29","author":"Liu","year":"2021","journal-title":"J. Agric. Biotechnol."},{"key":"ref_72","unstructured":"Shi, J. (2023). Transcriptomics-Based Analysis of the Differential Expressed Genes and Functional Exploration of GRB10 and GRB14 Genes in Yaks Testises with Different Ages. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_73","first-page":"1656","article-title":"Distribution of Extracellular Matrix Components in Aging Yak Testis","volume":"46","author":"Yuan","year":"2015","journal-title":"Chin. Anim. Vet. Sci."},{"key":"ref_74","first-page":"2394","article-title":"Differential Expression of Adiponectin, Its Receptor and Monocarboxylate Transporter in Testis of Yaks at Different Ages","volume":"39","author":"Zhang","year":"2025","journal-title":"Nucl. Agric. Sci."},{"key":"ref_75","first-page":"203","article-title":"Cloning of Yak SIRT1 Gene and Its Expression Pattern in Testis at Different Development Stages","volume":"35","author":"Yin","year":"2020","journal-title":"Acta Agric. Boreali-Sin."},{"key":"ref_76","first-page":"6","article-title":"Molecular Characteristics of TNP2 Gene in Yaks and Its Quantification in Testis at Different Developmental Stages","volume":"53","author":"Lin","year":"2021","journal-title":"Anim. Sci. Vet. Med."},{"key":"ref_77","first-page":"715","article-title":"Morphological Characteristics and Vascular Casts of Testes in Yak and Cattle","volume":"53","author":"Wang","year":"2018","journal-title":"Chin. J. Zool."},{"key":"ref_78","first-page":"29","article-title":"Analysis of Differences in Semen Quality of Bulls From Different Breeds of Yaks","volume":"55","author":"Wang","year":"2025","journal-title":"Chin. Qinghai J. Anim. Vet. Sci."},{"key":"ref_79","first-page":"271","article-title":"Effects of Different Seasons and Breeds on Semen Quality of Bulls in Tibetan Areas","volume":"61","author":"Ciren","year":"2025","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_80","first-page":"3475","article-title":"Differences in Expression of HSPA2 in Different Tissues and Organs of Yak","volume":"47","author":"Wen","year":"2014","journal-title":"Sci. Agric. Sin."},{"key":"ref_81","unstructured":"Sun, H.M. (2018). Observation and Comparison of Morphology of Testis Arteries in Tibetan Sheep and Yaks. [Master\u2019s Thesis, Gansu Agricultural University]."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1111\/ahe.12822","article-title":"Comparison study of morphology and vascular casts between plateau-type Tibetan sheep and low-altitude small-tailed Han sheep testes","volume":"51","author":"Liu","year":"2022","journal-title":"Anat. Histol. Embryol."},{"key":"ref_83","first-page":"375","article-title":"Anatomical Characteristics of Testicular Lobule and Epididymal Arterioles in Tibetan Sheep and Small-Tailed Han Sheep","volume":"54","author":"Yang","year":"2019","journal-title":"Chin. J. Zool."},{"key":"ref_84","first-page":"30","article-title":"Study on Growth and Development of Reproductive Organs in Tibetan Pigs","volume":"36","author":"Zeng","year":"2009","journal-title":"Sichuan Anim. Vet. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1017\/S1751731118000435","article-title":"Review: Spermatogenesis in the bull","volume":"12","author":"Staub","year":"2018","journal-title":"Animal"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s11427-019-1572-7","article-title":"Recent advances in mammalian reproductive biology","volume":"63","author":"Wen","year":"2019","journal-title":"Sci. China Life Sci."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-0-387-09597-4_1","article-title":"Spermatogenesis and cycle of the seminiferous epithelium","volume":"636","author":"Hess","year":"2009","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Yao, M., Qu, H., Han, Y., Cheng, C.Y., and Xiao, X. (2022). Kinesins in Mammalian Spermatogenesis and Germ Cell Transport. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.837542"},{"key":"ref_89","first-page":"8","article-title":"The Cycle of the Seminiferous Epithelium in Yak","volume":"3","author":"Xia","year":"1990","journal-title":"China Yak"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/aja.1001400204","article-title":"The cycle of the seminiferous epithelium and spermatogenesis in the bovine testis","volume":"140","author":"Berndston","year":"1974","journal-title":"Am. J. Anat."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.theriogenology.2019.04.016","article-title":"GDNF family receptor alpha 1 is a reliable marker of undifferentiated germ cells in bulls","volume":"132","author":"Kim","year":"2019","journal-title":"Theriogenology"},{"key":"ref_92","first-page":"1603","article-title":"Isolation, Culture, and Comparison of Biological Characteristics of Sertoli Cells from Yak and Cattle-Yak","volume":"52","author":"Chen","year":"2021","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Wu, S., Mipam, T., Xu, C., Zhao, W., Shah, M.A., Yi, C., Luo, H., Cai, X., and Zhong, J. (2020). Testis transcriptome profiling identified genes involved in spermatogenic arrest of cattleyak. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0229503"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"E70136","DOI":"10.1111\/rda.70136","article-title":"Reduced OTUD6A Impairs Spermatogonia Proliferation and Contributes to Spermatogenic Arrest in Cattleyak","volume":"60","author":"Dong","year":"2025","journal-title":"Reprod. Domest. Anim."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"479","DOI":"10.4103\/aja.aja_26_18","article-title":"Stimulated by retinoic acid gene 8 (Stra8) plays important roles in many stages of spermatogenesis","volume":"20","author":"Zheng","year":"2018","journal-title":"Asian J. Androl."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1146\/annurev.genet.33.1.603","article-title":"Meiotic chromosomes: Integrating structure and function","volume":"33","author":"Zickler","year":"1999","journal-title":"Annu. Rev. Genet."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/aja.2011.93","article-title":"The X and Y chromosome in meiosis: How and why they keep silent","volume":"13","author":"Eijpe","year":"2011","journal-title":"Asian J. Androl."},{"key":"ref_98","unstructured":"Hong, R. (2024). Research on Expression Regulation of Alternative Splicing and Transcription Factor MYBL1 in Dzo Male Sterility. [Master\u2019s Thesis, Southwest University]."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"100471","DOI":"10.1016\/j.vas.2025.100471","article-title":"Molecular characterization and expression of Spata22 in testes of the sterile male cattle-yak","volume":"29","author":"Niayale","year":"2025","journal-title":"Vet. Anim. Sci."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1111\/rda.14338","article-title":"Localization and expression of SLX4 in the testis of sterile male cattle-yak","volume":"58","author":"Zhao","year":"2023","journal-title":"Reprod. Domest. Anim."},{"key":"ref_101","first-page":"666","article-title":"Comparison of the mRNA Expression Level of ERK1, ERK2 and P38 Genes in the Testes of Yaks and Sterile Cattle-yaks","volume":"35","author":"Lei","year":"2016","journal-title":"Sichuan J. Zool."},{"key":"ref_102","first-page":"495","article-title":"Expression levels of Cdc2 and Cdc25A mRNA in cattle, yak, and cattle-yak testis","volume":"31","author":"Dong","year":"2009","journal-title":"Hereditas"},{"key":"ref_103","first-page":"3126","article-title":"Sequence Characteristics and Comparative Expression Analysis in Testis of ESCO2 Gene in Cattle-yak and Yak","volume":"52","author":"Min","year":"2021","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Wittayarat, M., Kawanishi, K., Ohata, H., Nagahara, M., Sambuu, R., Sambuu, O., Hirata, M., Tanihara, F., Taniguchi, M., and Otoi, T. (2025). Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle-Yak. Animals, 15.","DOI":"10.3390\/ani15050660"},{"key":"ref_105","first-page":"279","article-title":"Cloning of SPATA3 Gene and Its Differential Expression Analysis in Yak and Cattle-yak Testis","volume":"51","author":"Huang","year":"2020","journal-title":"Acta Vet. Zootech. Sin."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"5683","DOI":"10.1038\/s41467-020-19414-4","article-title":"Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells","volume":"11","author":"Zhao","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1038\/s41422-018-0099-2","article-title":"The adult human testis transcriptional cell atlas","volume":"28","author":"Guo","year":"2018","journal-title":"Cell Res."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"e21187","DOI":"10.1096\/fj.202001035RRR","article-title":"Characterization of sheep spermatogenesis through single-cell RNA sequencing","volume":"35","author":"Yang","year":"2020","journal-title":"FASEB J."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"e12502","DOI":"10.1002\/rmb2.12502","article-title":"Overview of single-cell RNA sequencing analysis and its application to spermatogenesis research","volume":"22","author":"Suzuki","year":"2023","journal-title":"Reprod. Med. Biol."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"6226","DOI":"10.1210\/en.2008-0349","article-title":"Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes","volume":"149","author":"Basciani","year":"2008","journal-title":"Endocrinology"},{"key":"ref_111","first-page":"2","article-title":"Sertoli cells as key drivers of testis function","volume":"121","author":"Smith","year":"2021","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1038\/sj.cdd.4401046","article-title":"Inhibition of sperm production in mice by annexin V microinjected into seminiferous tubules: Possible etiology of phagocytic clearance of apoptotic spermatogenic cells and male infertility","volume":"9","author":"Maeda","year":"2002","journal-title":"Cell Death Differ."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1002\/mrd.20278","article-title":"In vivo analysis of phagocytosis of apoptotic cells by testicular Sertoli cells","volume":"71","author":"Nakagawa","year":"2005","journal-title":"Mol. Reprod. Dev."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.stem.2019.12.005","article-title":"The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty","volume":"26","author":"Guo","year":"2020","journal-title":"Cell Stem Cell"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.mce.2016.08.033","article-title":"Expression profiling identifies Sertoli and Leydig cell genes as Fsh targets in adult zebrafish testis","volume":"437","author":"Crespo","year":"2016","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"E13087","DOI":"10.1111\/jne.13087","article-title":"Development of the gonadotropin-releasing hormone system","volume":"34","author":"Duittoz","year":"2021","journal-title":"J. Neuroendocrinol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.semcdb.2014.02.012","article-title":"The regulation of spermatogenesis by androgens","volume":"30","author":"Smith","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1530\/REP-16-0588","article-title":"Role of the testis interstitial compartment in spermatogonial stem cell function","volume":"153","author":"Potter","year":"2017","journal-title":"Reproduction"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1111\/andr.12703","article-title":"Essential roles of interstitial cells in testicular development and function","volume":"8","author":"Heinrich","year":"2019","journal-title":"Andrology"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.jgg.2022.03.014","article-title":"Single-cell RNA-seq analysis of testicular somatic cell development in pigs","volume":"49","author":"Zhang","year":"2022","journal-title":"J. Genet. Genom."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/BF01553493","article-title":"DNA methylation changes during mouse spermatogenesis","volume":"2","author":"Prantera","year":"1994","journal-title":"Chromosome Res."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.theriogenology.2020.01.001","article-title":"The expression of histone methyltransferases and distribution of selected histone methylations in testes of yak and cattle-yak hybrid","volume":"144","author":"Li","year":"2020","journal-title":"Theriogenology"},{"key":"ref_123","first-page":"752","article-title":"DNA methylation in male sterility of cattle-yak","volume":"37","author":"Zhao","year":"2017","journal-title":"Chin. J. Vet. Sci."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1080\/15592294.2020.1738026","article-title":"Promoter hypermethylation of PIWI\/piRNA pathway genes associated with diminished pachytene piRNA production in bovine hybrid male sterility","volume":"15","author":"Zhang","year":"2020","journal-title":"Epigenetics"},{"key":"ref_125","first-page":"939","article-title":"Methylation and acetylation of histones during spermatogenesis","volume":"33","author":"Ge","year":"2011","journal-title":"Hereditas"},{"key":"ref_126","first-page":"181","article-title":"The role of epigenetics in spermatogenesis","volume":"39","author":"Gunes","year":"2013","journal-title":"T\u00fcrk Urol. Derg.\/Turk. J. Urol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1126\/science.1183439","article-title":"PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice","volume":"327","author":"Baudat","year":"2009","journal-title":"Science"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1038\/nature04112","article-title":"A histone H3 methyltransferase controls epigenetic events required for meiotic prophase","volume":"438","author":"Hayashi","year":"2005","journal-title":"Nature"},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Yan, Q., and Wang, Q. (2025). Exploring the Characters of Non-Coding RNAs in Spermatogenesis and Male Infertility. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26031128"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nature11247","article-title":"An integrated encyclopedia of DNA elements in the human genome","volume":"489","author":"Dunham","year":"2012","journal-title":"Nature"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s00018-024-05399-6","article-title":"The functions and mechanisms of piRNAs in mediating mammalian spermatogenesis and their applications in reproductive medicine","volume":"81","author":"Du","year":"2024","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_132","doi-asserted-by":"crossref","unstructured":"Li, H., Li, Y., Wang, Y., Liu, L., Cui, J., Zhang, M., and Yan, Y. (2025). Genome-wide landscape of miRNA-mRNA-lncRNA-circRNA ceRNA network in Nanos2 deficient mice. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0325260"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"107360","DOI":"10.1016\/j.anireprosci.2023.107360","article-title":"Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity","volume":"258","author":"Sahlu","year":"2023","journal-title":"Anim. Reprod. Sci."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"105921","DOI":"10.1016\/j.livsci.2026.105921","article-title":"Hypoxia adaptation in yak: Molecular, Genetic, and Physiological mechanisms of high-altitude resilience","volume":"307","author":"Shahid","year":"2026","journal-title":"Livest. Sci."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"7783","DOI":"10.1073\/pnas.1322909111","article-title":"DNMT3a epigenetic program regulates the HIF-2alpha oxygen-sensing pathway and the cellular response to hypoxia","volume":"111","author":"Lachance","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1093\/nsr\/nwz213","article-title":"Convergent genomic signatures of high-altitude adaptation among domestic mammals","volume":"7","author":"Wu","year":"2019","journal-title":"Natl. Sci. Rev."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/S2095-3119(14)60854-6","article-title":"Novel SNP of EPAS1 gene associated with higher hemoglobin concentration revealed the hypoxia adaptation of yak (Bos grunniens)","volume":"14","author":"Wu","year":"2015","journal-title":"J. Integr. Agric."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1095\/biolreprod.109.079202","article-title":"EPAS1 Is Required for Spermatogenesis in the Postnatal Mouse Testis","volume":"82","author":"Gruber","year":"2010","journal-title":"Biol. Reprod."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"7317","DOI":"10.3168\/jds.2023-24442","article-title":"Single-cell analysis identifies critical regulators of spermatogonial development and differentiation in cattle-yak bulls","volume":"107","author":"Zhang","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_140","doi-asserted-by":"crossref","unstructured":"Cao, M., Pei, J., Xiong, L., Guo, S., Wang, X., Kang, Y., and Guo, X. (2022). Analysis of Chromatin Openness in Testicle Tissue of Yak and Cattle-Yak. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232415810"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"19903","DOI":"10.1038\/s41598-024-69458-5","article-title":"Whole transcriptome sequencing revealed the gene regulatory network of hypoxic response in yak Sertoli cells","volume":"14","author":"Ma","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Zhao, S., Chen, T., Luo, X., Chen, S., Wang, J., Lai, S., and Jia, X. (2021). Identification of Novel lncRNA and Differentially Expressed Genes (DEGs) of Testicular Tissues among Cattle, Yak, and Cattle-Yak Associated with Male Infertility. Animals, 11.","DOI":"10.3390\/ani11082420"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0378-4320(00)00153-6","article-title":"Storage of bovine semen in liquid and frozen state","volume":"62","author":"Vishwanath","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1021\/acs.jproteome.2c00528","article-title":"Identification and Validation of Yak (Bos grunniens) Frozen\u2013Thawed Sperm Proteins Associated with Capacitation and the Acrosome Reaction","volume":"21","author":"Zhang","year":"2022","journal-title":"J. Proteome Res."},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Kalwar, Q., Chu, M., Korejo, R.A., Soomro, H., and Yan, P. (2022). Cryopreservation of Yak Semen: A Comprehensive Review. Animals, 12.","DOI":"10.3390\/ani12243451"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"607","DOI":"10.56093\/ijans.v85i6.49319","article-title":"Effect of equilibration period on post thaw semen quality of Indian yak","volume":"85","author":"Borah","year":"2015","journal-title":"Indian J. Anim. Sci."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"428","DOI":"10.56093\/ijans.v88i4.78805","article-title":"Effect of different glycerol levels in tris-based extender on quality of frozen yak semen","volume":"88","author":"Borah","year":"2018","journal-title":"Indian J. Anim. Sci."},{"key":"ref_148","doi-asserted-by":"crossref","unstructured":"Zhu, Y., Yu, J., Li, X., Chen, Z., Li, Y., Xiong, Y., He, H., Yin, S., Lan, D., and Li, J. (2025). Supplementation of DHA enhances the cryopreservation of yak semen via alleviating oxidative stress and inhibiting apoptosis. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1532473"},{"key":"ref_149","doi-asserted-by":"crossref","unstructured":"Li, X., Yu, J., Li, Y., Chen, Z., Zeng, R., Cen, Y., Wang, Y., Zhang, C., Zhang, D., and Yin, S. (2026). Ginseng Peptide Improves the Cryopreservation Efficiency and Fertilization Potential of Yak Semen via FOXO1\/PI3K\/AKT Axis. Antioxidants, 15.","DOI":"10.3390\/antiox15020156"},{"key":"ref_150","doi-asserted-by":"crossref","unstructured":"Fan, Y., Lv, W., Li, X., Wanma, G., Li, J., Xiong, X., He, X., Wang, Y., Yin, S., and Fu, W. (2025). Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1613506"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.anireprosci.2008.12.012","article-title":"Season of the year influences semen output and concentrations of testosterone in circulation of yaks (Poephagus grunniens L.)","volume":"115","author":"Sarkar","year":"2009","journal-title":"Anim. Reprod. Sci."},{"key":"ref_152","first-page":"60","article-title":"Study on the Selective Breeding of Local Varieties of Pari Yak","volume":"2","author":"Ji","year":"2011","journal-title":"Tibet Sci. Technol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2730\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:05:57Z","timestamp":1788336357000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":152,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16172730"],"URL":"https:\/\/doi.org\/10.3390\/ani16172730","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}