{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:18:11Z","timestamp":1788499091770,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2023YFD1300804-2"],"award-info":[{"award-number":["2023YFD1300804-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>This study evaluated graded dietary supplementation with sterol-enriched yeast hydrolysate (SYH) in late-laying yellow-feathered broiler breeder hens. A total of 720 55-week-old hens received 0, 0.2, 0.5, or 0.8 g\/kg of SYH for six weeks after a one-week adaptation period. SYH is a composite product containing sterols, mannan, nucleotides, and amino nitrogen; therefore, the observed effects cannot be attributed to sterols alone. Eggshell strength increased at 0.5 and 0.8 g\/kg, whereas fertility and hatchability were unaffected. The 0.5 g\/kg group showed the highest ovarian index values and the greatest quantities of 5\u201312 mm yellow follicles. The 0.8 g\/kg group showed the highest serum reproductive hormone and immunoglobulin concentrations, together with the highest reactive oxygen species and cytokine concentrations. Hepatic and ovarian superoxide dismutase activity increased selectively, whereas most other antioxidant indices were unchanged. Ovarian FSHR, CYP19A1, and VDR mRNA expression showed concentration-specific patterns. The strongest peripheral endocrine response at 0.8 g\/kg did not coincide with the most favorable ovarian developmental response. Under the conditions of this study, 0.5 g\/kg represents a candidate inclusion concentration for further validation rather than a confirmed optimum.<\/jats:p>","DOI":"10.3390\/ani16172750","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:02:52Z","timestamp":1788343372000},"page":"2750","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Dietary Sterol-Enriched Yeast Hydrolysate on Ovarian Development, Reproductive Endocrine Status, Immune-Inflammatory Responses, Antioxidant Indices, and Egg Quality in Late-Laying Yellow-Feathered Broiler Breeder Hens"],"prefix":"10.3390","volume":"16","author":[{"given":"Haoran","family":"Si","sequence":"first","affiliation":[{"name":"Animal Science, Xinjiang Agricultural University, No. 311 Nongda East Road, Urumqi 830052, 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China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2619-2090","authenticated-orcid":false,"given":"Bo","family":"Fan","sequence":"additional","affiliation":[{"name":"Animal Science, Xinjiang Agricultural University, No. 311 Nongda East Road, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xuanyang","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Science, Henan University of Animal Husbandry and Economy, No. 6 Longzihu North Road, Zhengzhou 450046, 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Reprod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1439-0531.2012.02087.x","article-title":"Follicle selection in the avian ovary","volume":"47","author":"Johnson","year":"2012","journal-title":"Reprod. Domest. Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1530\/rep.0.1230721","article-title":"Differentiation of chicken gonad as an endocrine organ: Expression of LH receptor, FSH receptor, cytochrome P450c17 and aromatase genes","volume":"123","author":"Akazome","year":"2002","journal-title":"Reproduction"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1016\/j.psj.2019.12.001","article-title":"Effect of curcumin on laying performance, egg quality, endocrine hormones, and immune activity in heat-stressed hens","volume":"99","author":"Liu","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40104-016-0097-5","article-title":"Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals","volume":"7","author":"Akbarian","year":"2016","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Liu, Y., Cheng, X., Zhen, W., Zeng, D., Qu, L., Wang, Z., and Ning, Z. (2021). Yeast culture improves egg quality and reproductive performance of aged breeder layers by regulating gut microbes. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.633276"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1016\/j.psj.2019.11.017","article-title":"Yeast culture promotes the production of aged laying hens by improving intestinal digestive enzyme activities and the intestinal health status","volume":"99","author":"Zhang","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Comi, M., Lanzoni, D., Perricone, V., Jiang, X.-R., Lin, J., and Zhang, H.-J. (2025). Effects of Saccharomyces cerevisiae hydrolysate on broiler performance and gut health. Animals, 15.","DOI":"10.3390\/ani15172531"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1080\/09712119.2020.1825446","article-title":"Egg production, egg quality, nutrient digestibility, and excreta microflora of laying hens fed with a diet containing brewer\u2019s yeast hydrolysate","volume":"48","author":"Park","year":"2020","journal-title":"J. Appl. Anim. Res."},{"key":"ref_10","unstructured":"(2018). Determination of Crude Protein in Feeds\u2014Kjeldahl Method (Standard No. GB\/T 6432-2018)."},{"key":"ref_11","unstructured":"(2018). Determination of Crude Fat in Feeds (Standard No. GB\/T 6433-2006)."},{"key":"ref_12","unstructured":"(2018). Determination of Calcium in Feeds (Standard No. GB\/T 6436-2018)."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Qiu, Q., Zhan, Z., Zhou, Y., Zhang, W., Gu, L., Wang, Q., He, J., Liang, Y., Zhou, W., and Li, Y. (2024). Effects of yeast culture on laying performance, antioxidant properties, intestinal morphology, and intestinal flora of laying hens. Antioxidants, 13.","DOI":"10.3390\/antiox13070779"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"104979","DOI":"10.1016\/j.psj.2025.104979","article-title":"Dietary Saccharomyces cerevisiae fermentation product improved egg quality by modulating intestinal health, ovarian function, and cecal microbiota in post-peak laying hens","volume":"104","author":"Gong","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.ijbiomac.2020.05.134","article-title":"Dietary yeast \u03b2-glucan supplementation improves eggshell color and fertile eggs hatchability as well as enhances immune functions in breeder laying hens","volume":"159","author":"Zhen","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.ijbiomac.2022.10.133","article-title":"Yeast cell-wall polysaccharides improve immunity and attenuate inflammatory response via modulating gut microbiota in LPS-challenged laying hens","volume":"224","author":"Zhou","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1501\/Vetfak_0000002644","article-title":"Effects of dietary yeast cell wall on performance, egg quality and humoral immune response in laying hens","volume":"61","author":"Eser","year":"2014","journal-title":"Ank. Univ. Vet. Fak. Derg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1002\/jsfa.4004","article-title":"Effects of dietary yeast autolysate (Saccharomyces cerevisiae) on performance, egg traits, egg cholesterol content, egg yolk fatty acid composition and humoral immune response of laying hens","volume":"90","author":"Eltan","year":"2010","journal-title":"J. Sci. Food Agric."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101745","DOI":"10.1016\/j.psj.2022.101745","article-title":"Performance and environmental impact of egg production in response to dietary supplementation of mannan oligosaccharide in laying hens: A meta-analysis","volume":"101","author":"Salami","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"txae123","DOI":"10.1093\/tas\/txae123","article-title":"Mannan oligosaccharides as a prebiotic for laying hens: Effects on fertility, hatchability, productive performance, and immunity","volume":"8","author":"Youssef","year":"2024","journal-title":"Transl. Anim. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1095\/biolreprod55.5.1055","article-title":"Characterization of the chicken follicle-stimulating hormone receptor (cFSH-R) complementary deoxyribonucleic acid, and expression of cFSH-R messenger ribonucleic acid in the ovary","volume":"55","author":"You","year":"1996","journal-title":"Biol. Reprod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ygcen.2017.03.013","article-title":"Transcription of CYP19A1 is directly regulated by SF-1 in the theca cells of ovary follicles in chicken","volume":"247","author":"Wang","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1530\/rep.0.1250683","article-title":"Role of FSH and epidermal growth factor (EGF) in the initiation of steroidogenesis in granulosa cells associated with follicular selection in chicken ovaries","volume":"125","author":"Hernandez","year":"2003","journal-title":"Reproduction"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1095\/biolreprod.104.033902","article-title":"Regulation of follicle-stimulating hormone-receptor messenger RNA in hen granulosa cells relative to follicle selection","volume":"72","author":"Woods","year":"2005","journal-title":"Biol. Reprod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3382\/ps\/pev318","article-title":"Granulosa cell responsiveness to follicle stimulating hormone during early growth of hen ovarian follicles","volume":"95","author":"Johnson","year":"2016","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100959","DOI":"10.1016\/j.psj.2020.12.056","article-title":"Effect of anti-M\u00fcllerian hormone on the development and selection of ovarian follicle in hens","volume":"100","author":"Huang","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"bqac112","DOI":"10.1210\/endocr\/bqac112","article-title":"Leptin promotes primordial follicle activation by regulating ovarian insulin-like growth factor system in chicken","volume":"163","author":"Ahmadi","year":"2022","journal-title":"Endocrinology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/1477-7827-12-25","article-title":"Leptin receptor signaling inhibits ovarian follicle development and egg laying in chicken hens","volume":"12","author":"Lei","year":"2014","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.3390\/cimb45050256","article-title":"1\u03b1,25-Dihydroxyvitamin D3 Improves Follicular Development and Steroid Hormone Biosynthesis by Regulating Vitamin D Receptor in the Layers Model","volume":"45","author":"Cheng","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.theriogenology.2017.08.002","article-title":"Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells","volume":"102","author":"Yao","year":"2017","journal-title":"Theriogenology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12986-018-0293-8","article-title":"Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress","volume":"15","author":"Geng","year":"2018","journal-title":"Nutr. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102660","DOI":"10.1016\/j.psj.2023.102660","article-title":"Dietary yeast cell wall enhanced intestinal health of broiler chickens by modulating intestinal integrity, immune responses, and microbiota","volume":"102","author":"Kyoung","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1080\/00071668.2020.1817328","article-title":"Yeast cell wall polysaccharides enhanced expression of T helper type 1 and 2 cytokines profile in chicken B lymphocytes exposed to LPS challenge and enzyme treatment","volume":"62","author":"Echeverry","year":"2021","journal-title":"Br. Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Luo, Q., Yang, L., Tumenjargal, B., Liu, S., Ma, J., Ning, J., Yun, Z., Zhang, X., Wu, Y., and Lu, Y. (2024). Effect of composite yeast culture on the jejunal barrier function, inflammatory response, and microbial community structure of laying hens during the late stage of egg production. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1524726"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"105690","DOI":"10.1016\/j.intimp.2019.105690","article-title":"Effect of sulfated yeast beta-glucan on cyclophosphamide-induced immunosuppression in chickens","volume":"74","author":"Wang","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"106222","DOI":"10.1016\/j.psj.2025.106222","article-title":"Selenium yeast improves egg production performance by increasing antioxidant capacity, reproductive hormone levels and yolk precursor synthesis in late peak laying hens","volume":"105","author":"Qin","year":"2026","journal-title":"Poult. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.cell.2020.01.009","article-title":"Single-cell transcriptomic atlas of primate ovarian aging","volume":"180","author":"Wang","year":"2020","journal-title":"Cell"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101891","DOI":"10.1016\/j.psj.2022.101891","article-title":"Naringin prevents follicular atresia by inhibiting oxidative stress in the aging chicken","volume":"101","author":"Bao","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"102745","DOI":"10.1016\/j.psj.2023.102745","article-title":"Melatonin alleviates ovarian function damage and oxidative stress induced by dexamethasone in the laying hens through FOXO1 signaling pathway","volume":"102","author":"Bai","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Yang, Z., Zhang, J., Yuan, Q., Wang, X., Zeng, W., Mi, Y., and Zhang, C. (2024). Flavonoid fisetin alleviates ovarian aging of laying chickens by enhancing antioxidant capacity and glucose metabolic homeostasis. Antioxidants, 13.","DOI":"10.3390\/antiox13121432"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1016\/j.psj.2019.11.040","article-title":"Melatonin regulates the ovarian function and enhances follicle growth in aging laying hens via activating the mammalian target of rapamycin pathway","volume":"99","author":"Hao","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Wang, J., Jia, R., Gong, H., Celi, P., Zhuo, Y., Ding, X., Bai, S., Zeng, Q., Yin, H., and Xu, S. (2021). The effect of oxidative stress on the chicken ovary: Involvement of microbiota and melatonin interventions. Antioxidants, 10.","DOI":"10.3390\/antiox10091422"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"102258","DOI":"10.1016\/j.psj.2022.102258","article-title":"Dietary supplementation with astaxanthin alleviates ovarian aging in aged laying hens by enhancing antioxidant capacity and increasing reproductive hormones","volume":"102","author":"He","year":"2023","journal-title":"Poult. Sci."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2750\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:42:06Z","timestamp":1788496926000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":43,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16172750"],"URL":"https:\/\/doi.org\/10.3390\/ani16172750","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]}}}