{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:16:33Z","timestamp":1788430593330,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Functional pet foods are increasingly being developed to support host metabolism and intestinal health. Lycium barbarum polysaccharide (LBP), a major bioactive component of goji berries, has been reported to possess antioxidant, anti-inflammatory, immunomodulatory, and gut microbiota-regulatory activities; however, its effects in domestic cats remain unclear. This study investigated the effects of dietary LBP supplementation on serum metabolomic profiles and gut microbiota in adult cats. Twenty-four healthy adult Chinese domestic cats (1.50 \u00b1 0.50 years old; 3.70 \u00b1 0.80 kg) were allocated to four groups using a body-weight-balanced procedure (n = 6 per group): a basal diet group (C) and basal diet supplemented with 0.2% (L), 0.3% (M), or 0.4% (H) LBP. After a 1-week adaptation period, the formal feeding trial lasted 8 weeks. Growth-related indices, health scores, hematological and serum biochemical parameters, untargeted serum metabolomics, and fecal 16S rRNA sequencing were evaluated. LBP supplementation did not significantly affect body weight, average daily feed intake, body condition score, fecal score, coat score, hematological indices, or serum biochemical parameters. Metabolomic analysis showed that LBP mainly affected lipid metabolism, unsaturated fatty acid biosynthesis, branched-chain amino acid metabolism, lysine degradation, and protein digestion and absorption-related pathways. Microbiota analysis showed changes in alpha diversity, community structure, and the relative abundance of taxa such as Olsenella, Bifidobacterium, Catenibacterium, Peptoclostridium, Collinsella, and Clostridium. Correlation analysis suggested potential associations between key bacterial genera and serum metabolites related to fatty acid and amino acid metabolism. Overall, dietary LBP altered serum metabolic signatures and gut microbial composition without causing adverse effects in adult cats, These exploratory findings require confirmation in larger feline studies and do not establish an optimal supplementation level.<\/jats:p>","DOI":"10.3390\/ani16172765","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:20:43Z","timestamp":1788427243000},"page":"2765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Lycium barbarum Polysaccharide Supplementation Modulates Serum Metabolomic Profiles and Gut Microbiota in Felines"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0246-373X","authenticated-orcid":false,"given":"Xiao","family":"Zhang","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Xinda","family":"Liu","sequence":"additional","affiliation":[{"name":"Gambol Pet Food Group Co., Ltd., Liaocheng 252027, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Weipeng","family":"Tian","sequence":"additional","affiliation":[{"name":"Gambol Pet Food Group Co., Ltd., Liaocheng 252027, 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Jackson, M.I., Ephraim, E., Badri, D.V., MacLeay, J.M., Jewell, D.E., and Suchodolski, J.S. (2020). The effects of nutrition on the gastrointestinal microbiome of cats and dogs: Impact on health and disease. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01266"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yang, B., Zhong, S., Wang, J., and Yu, W. (2025). Dietary Modulation of the Gut Microbiota in Dogs and Cats and Its Role in Disease Management. Microorganisms, 13.","DOI":"10.3390\/microorganisms13122669"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Teixeira, F., Silva, A.M., Delerue-Matos, C., and Rodrigues, F. (2023). Lycium barbarum berries (Solanaceae) as source of bioactive compounds for healthy purposes: A review. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054777"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Cao, C., Wang, Z., Gong, G., Huang, W., Huang, L., Song, S., and Zhu, B. (2022). Effects of Lycium barbarum polysaccharides on immunity and gut microbiota. Foods, 11.","DOI":"10.3390\/foods11203177"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1080\/10408398.2022.2128037","article-title":"Recent progress of Lycium barbarum polysaccharides on intestinal microbiota, microbial metabolites and health: A review","volume":"64","author":"Feng","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"109591","DOI":"10.1016\/j.biopha.2019.109591","article-title":"Prebiotic, immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide","volume":"121","author":"Zhu","year":"2020","journal-title":"Biomed. Pharmacother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xia, H., Zhou, B., Sui, J., Ma, W., Wang, S., Yang, L., and Sun, G. (2022). Lycium barbarum polysaccharide regulates the lipid metabolism and alters gut microbiota in high-fat diet induced obese mice. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph191912093"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s44149-024-00140-z","article-title":"Analytical insights, modulation and compositional dynamics of the feline gut microbiota: A review","volume":"4","author":"Shi","year":"2024","journal-title":"Anim. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s42523-022-00213-9","article-title":"Dietary enrichment of resistant starches or fibers differentially alter the feline fecal microbiome and metabolite profile","volume":"4","author":"Lee","year":"2022","journal-title":"Anim. Microbiome"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"W398","DOI":"10.1093\/nar\/gkae253","article-title":"MetaboAnalyst 6.0: Towards a unified platform for metabolomics data processing, analysis and interpretation","volume":"52","author":"Pang","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_12","first-page":"13","article-title":"Development and validation of a body condition score system for cats: A clinical tool","volume":"25","author":"Laflamme","year":"1997","journal-title":"Feline Pract."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1038\/nprot.2011.335","article-title":"Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry","volume":"6","author":"Dunn","year":"2011","journal-title":"Nat. Protoc."},{"key":"ref_14","unstructured":"Broeckling, C.D., Ganna, A., Layer, M., Brown, K., Sutton, B., Ingelsson, E., Peers, G., and Prenni, J.E. (2023). Current practices in LC-MS untargeted metabolomics: A scoping review. Metabolites, 13."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1038\/s41596-022-00710-w","article-title":"Using MetaboAnalyst 5.0 for LC-HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data","volume":"17","author":"Chong","year":"2022","journal-title":"Nat. Protoc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e100","DOI":"10.1002\/cpbi.100","article-title":"QIIME 2 enables comprehensive end-to-end analysis of diverse microbiome data and comparative studies with publicly available data","volume":"70","author":"Estaki","year":"2020","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_19","unstructured":"National Research Council (2006). Nutrient Requirements of Dogs and Cats, National Academies Press."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.2460\/javma.2002.221.1559","article-title":"The carnivore connection to nutrition in cats","volume":"221","author":"Zoran","year":"2002","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"18S","DOI":"10.1177\/0148607115595980","article-title":"Functional roles of fatty acids and their effects on human health","volume":"39","author":"Calder","year":"2015","journal-title":"JPEN J. Parenter. Enter. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Rohrhofer, J., Zwirzitz, B., Selberherr, E., and Untersmayr, E. (2021). The impact of dietary sphingolipids on intestinal microbiota and gastrointestinal immune homeostasis. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.635704"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Pa\u00dflack, N., B\u00fcttner, K., Vahjen, W., and Zentek, J. (2025). Impact of the Dietary Fat Concentration and Source on the Fecal Microbiota of Healthy Adult Cats. Metabolites, 15.","DOI":"10.3390\/metabo15040215"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00726-009-0269-0","article-title":"Amino acids: Metabolism, functions, and nutrition","volume":"37","author":"Wu","year":"2009","journal-title":"Amino Acids"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Jewell, D.E., Jackson, M.I., Cochrane, C.Y., and Badri, D.V. (2022). Feeding fiber-bound polyphenol ingredients at different levels modulates colonic postbiotics to improve gut health in cats. Animals, 12.","DOI":"10.3390\/ani12131654"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Giordano, M.V., Crisi, P.E., Gramenzi, A., Cattaneo, D., Corna, L., Sung, C.H., Tolbert, K.M., Steiner, J.M., Suchodolski, J.S., and Boari, A. (2024). Fecal microbiota and concentrations of long-chain fatty acids, sterols, and unconjugated bile acids in cats with chronic enteropathy. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1401592"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sung, C.H., Pilla, R., Marsilio, S., Chow, B., Zornow, K.A., Slovak, J.E., Lidbury, J.A., Steiner, J.M., Hill, S.L., and Suchodolski, J.S. (2023). Fecal Concentrations of Long-Chain Fatty Acids, Sterols, and Unconjugated Bile Acids in Cats with Chronic Enteropathy. Animals, 13.","DOI":"10.3390\/ani13172753"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ma, Q., Zhai, R., Xie, X., Chen, T., Zhang, Z., Liu, H., Nie, C., Yuan, X., Tu, A., and Tian, B. (2022). Hypoglycemic Effects of Lycium barbarum Polysaccharide in Type 2 Diabetes Mellitus Mice via Modulating Gut Microbiota. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.916271"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Yan, A., Ding, H., Liu, J., Bi, C., Han, Z., Wang, Z., Nawaz, S., Shen, Y., and Liu, S. (2023). Black Lycium barbarum polysaccharide attenuates LPS-induced intestine damage via regulation gut microbiota. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1080922"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7209","DOI":"10.1039\/D3FO01659E","article-title":"Integrated gut microbiota and fecal metabolome analyses of the effect of Lycium barbarum polysaccharide on D-galactose-induced premature ovarian insufficiency","volume":"14","author":"Zheng","year":"2023","journal-title":"Food Funct."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bugrov, N., Rudenko, P., Lutsay, V., Gurina, R., Zharov, A., Khairova, N., Molchanova, M., Krotova, E., Shopinskaya, M., and Bolshakova, M. (2022). Fecal microbiota analysis in cats with intestinal dysbiosis of varying severity. Animals, 12.","DOI":"10.3390\/pathogens11020234"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.chom.2023.01.009","article-title":"Gut microbiome lipid metabolism and its impact on host physiology","volume":"31","author":"Brown","year":"2023","journal-title":"Cell Host Microbe"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"11660","DOI":"10.1038\/s41598-024-62243-4","article-title":"Gut microbiota promoting propionic acid production is associated with weight loss in obese cats","volume":"14","author":"Rowe","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.psj.2019.10.043","article-title":"Effects of dietary Lycium barbarum polysaccharides on growth performance, digestive enzyme activities, antioxidant status and immunity of broiler chickens","volume":"99","author":"Long","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"123500","DOI":"10.1016\/j.ijbiomac.2023.123500","article-title":"Dietary supplementation with Lycium barbarum polysaccharides conducive to maintaining the health of Luciobarbus capito via the enhancement of enzyme activities and the modulation of gut microbiota","volume":"232","author":"Sun","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1039\/C9FO00638A","article-title":"Modulating effects of polysaccharides from the fruits of Lycium barbarum on the immune response and gut microbiota in cyclophosphamide-treated mice","volume":"10","author":"Ding","year":"2019","journal-title":"Food Funct."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.cbpa.2017.01.001","article-title":"How close are we to complete annotation of metabolomes?","volume":"36","author":"Viant","year":"2017","journal-title":"Curr. Opin. Chem. Biol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2765\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:37:19Z","timestamp":1788428239000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":37,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16172765"],"URL":"https:\/\/doi.org\/10.3390\/ani16172765","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]}}}