{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:13:43Z","timestamp":1788527623203,"version":"build-2803163510"},"reference-count":27,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yuelushan Laboratory Breeding Program","award":["YLS-2025-ZY02028"],"award-info":[{"award-number":["YLS-2025-ZY02028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Accurate pedigree reconstruction is critical for genetic evaluation in admixed cattle populations, yet the relative performance of microsatellite and genome-wide SNP markers in twin-rich herds with incomplete pedigree records remains unclear. We compared 12 ISAG-recommended microsatellite markers with whole-genome SNP data for dam\u2013calf assignment in a Simmental crossbred population (n = 43, 13 dam\u2013calf groups) from southern China. Twin zygosity was determined from SNP identity-by-descent (PI_HAT) values: nine calf pairs were dizygotic, one pair was monozygotic (20A\/21A), and one adult pair was composed of dizygotic twin sisters (31A\/34A). Admixture analysis at K = 3 revealed ancestry proportions of 50.7% European taurine, 28.9% Chinese indicine and 20.4% East Asian taurine. The SNP-based neighbor-joining tree correctly recovered 12 of 13 groups (92.3%, 95% CI: 64.0\u201399.8%), whereas the microsatellite-based tree recovered 11 (84.6%, 95% CI: 54.6\u201398.1%); the difference was not statistically significant (exact McNemar test, p = 1.0). Locus INRA023 was monomorphic (PIC = 0), reducing the effective number of markers to 11. These results indicate that genome-wide SNPs show a favourable trend in accuracy and are less prone to false-positive clustering than a standard microsatellite panel in admixed, twin-rich cattle populations.<\/jats:p>","DOI":"10.3390\/ani16172780","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:20:46Z","timestamp":1788524446000},"page":"2780","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microsatellites Versus Genome-Wide SNPs Data for Pedigree Reconstruction in Twin Simmental Crossbred Cattle"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7377-3135","authenticated-orcid":false,"given":"Xinyu","family":"Chen","sequence":"first","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"},{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Li","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7659-6777","authenticated-orcid":false,"given":"Yang","family":"Luo","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baizhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"He","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1647-1037","authenticated-orcid":false,"given":"Chuzhao","family":"Lei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naqash","family":"Khan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangle","family":"Yi","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"Hunan Institute of Animal and Veterinary Science, Changsha 410000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.22271\/j.ento.2020.v8.i6e.7876","article-title":"Monozygous twinning in cattle: A case study","volume":"8","author":"Chakrabarti","year":"2020","journal-title":"J. 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