{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:13:12Z","timestamp":1788541992246,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["CARS-21"],"award-info":[{"award-number":["CARS-21"]}]},{"award":["CARS-21"],"award-info":[{"award-number":["CARS-21"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The global transition to antibiotic-free livestock production, accelerated by antimicrobial-resistance concerns, has intensified the search for alternatives to antibiotic growth promoters, and phytobiotics have emerged as key candidates. Cangzhu, the dried rhizome of Atractylodes lancea (Thunb.) DC, is a crude drug of traditional Chinese medicine that has attracted substantial attention as a multipurpose phytobiotic. This review synthesizes current knowledge on the phytochemistry, gut-protective mechanisms, and practical application of Cangzhu in sustainable livestock production. Its principal bioactive constituents are sesquiterpenoids (atractylone, \u03b2-eudesmol, hinesol, atractylenolides I\u2013III), polyacetylenes (including atractylodin), polysaccharides, and flavonoids. Reported gut-protective mechanisms comprise upregulation of tight junction proteins (ZO-1, occludin, claudin-1); dual suppression of the NF-\u03baB and MAPK-signaling pathways; activation of the Nrf2\u2013Keap1 antioxidant axis; modulation of the gut microbiota; and TLR4-mediated stimulation of mucosal immunity. In swine, poultry, and ruminant studies, Atractylodes-based supplementation has been associated with improved growth performance, a lower incidence of diarrhea, favorable intestinal morphology, downregulated pro-inflammatory cytokines (TNF-\u03b1, IL-1\u03b2, IL-6), beneficial microbiota shifts, and inhibited pathogens. However, much of the mechanistic evidence derives from rodent and in vitro models, and much of the in vivo livestock evidence from the related species A. macrocephala (Baizhu) or multi-herb formulations; direct livestock trials with pure A. lancea remain limited for several endpoints, and the responses observed in these heterogeneous studies depend on the formulation, dose, and animal species used, as indicated by the explicit evidence-tier and source-species tags applied throughout the text, tables, and figures. The reported effective inclusion levels should be read as experimental study ranges and not as standardized dose recommendations. Framed within the One Health paradigm, Cangzhu is therefore best characterized as a mechanistically substantiated candidate alternative to antibiotic growth promoters whose adoption requires species-specific dose validation, quality standardization, and commercial-scale economic confirmation.<\/jats:p>","DOI":"10.3390\/ani16172789","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:07:32Z","timestamp":1788538052000},"page":"2789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Harnessing Phytobiotics for Gut Protection: A Comprehensive Review on the Role of Cangzhu (Atractylodes lancea) in Sustainable Animal Husbandry"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2240-0212","authenticated-orcid":false,"given":"Mahmoud","family":"Soliman","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100084, China"},{"name":"Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El Sheikh 35111, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Du","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangwenyao","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hejian","family":"Song","sequence":"additional","affiliation":[{"name":"Animal Husbandry Workstation, Aba Tibetan and Qiang Autonomous Prefecture Bureau of Agriculture and Rural Affairs, Barkam 620992, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1246-4748","authenticated-orcid":false,"given":"Linli","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100084, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","unstructured":"FAO (2018). The Future of Food and Agriculture: Alternative Pathways to 2050, Food and Agriculture Organization of the United Nations."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1126\/science.1185383","article-title":"Food security: The challenge of feeding 9 billion people","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"ref_3","unstructured":"WHO (2014). Antimicrobial Resistance: Global Report on Surveillance, World Health Organization."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.1073\/pnas.1503141112","article-title":"Global trends in antimicrobial use in food animals","volume":"112","author":"Brower","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","unstructured":"MARA-PRC (2019). Announcement No. 194: Ban on the Use of Antibiotics as Feed Additives."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., and Yuan, Z. (2014). Antibiotic alternatives: The substitution of antibiotics in animal husbandry. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00217"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"E140","DOI":"10.2527\/jas.2007-0459","article-title":"Use of phytogenic products as feed additives for swine and poultry","volume":"86","author":"Windisch","year":"2008","journal-title":"J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1080\/1828051X.2017.1350120","article-title":"Phytobiotics in poultry and swine nutrition: A review","volume":"17","author":"Ogbuewu","year":"2018","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s40781-017-0133-9","article-title":"Dietary inclusion effects of phytochemicals as growth promoters","volume":"59","year":"2017","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Murugesan, G.R., Syed, B., Haldar, S., and Pender, C. (2015). Phytogenic feed additives as an alternative to antibiotic growth promoters in broiler chickens. Front. Vet. Sci., 2, Erratum in Front. Vet. Sci. 2015, 2, 37, Front. Vet. Sci. 2016, 3, 28.","DOI":"10.3389\/fvets.2015.00021"},{"key":"ref_12","unstructured":"Chinese Pharmacopoeia Commission (2020). Pharmacopoeia of the People\u2019s Republic of China, Vol. I, China Medical Science Press."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gao, Y., Wang, D., Ma, X., Li, J., Wang, D., Chen, B., Yang, X., and Leng, H. (2025). Biological functions of Atractylodes lancea and its applications in animal husbandry: A review. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1518433"},{"key":"ref_14","first-page":"17112","article-title":"Atractylodes lancea rhizome polysaccharide alleviates immunosuppression and intestinal mucosal injury in mice treated with cyclophosphamide","volume":"71","author":"Wang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1142\/S0192415X22500203","article-title":"Atractylodes lancea Rhizoma attenuates DSS-induced colitis by regulating intestinal flora and metabolites","volume":"50","author":"Qu","year":"2022","journal-title":"Am. J. Chin. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S1995-7645(14)60069-9","article-title":"Therapeutic potential and pharmacological activities of Atractylodes lancea (Thunb.) DC","volume":"7","author":"Koonrungsesomboon","year":"2014","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhang, G., Zhao, Z., Li, Y., Zhang, L., Lu, J., Qi, K., Liang, H., Zha, L., and Xie, J. (2025). Transcriptome and metabolome analysis of Atractylodes lancea across different developmental stages. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1720423"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Tian, J., Zhang, G., He, N., Tan, L., Wu, J., Chen, Y., Yao, L., Bi, Y., Zhaxi, Y., and Lu, J. (2026). Glycyrrhiza uralensis\u2013Atractylodes macrocephala combination supplementation enhances broiler growth performance, immunity, and intestinal health, potentially mediated through gut microbiota and metabolites. Front. Vet. Sci., 13.","DOI":"10.3389\/fvets.2026.1855302"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Qiu, H., Shan, C., Fei, C., Xue, P., Zhou, Y., Yuan, J., and Liu, X. (2023). Geoherbalism metabolomic analysis of Atractylodes lancea (Thunb.) DC. by LC-Triple TOF-MS\/MS and GC-MS. Molecules, 28.","DOI":"10.3390\/molecules28165974"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e2000268","DOI":"10.1002\/cbdv.202000268","article-title":"Antioxidant, antimicrobial and anti-inflammatory activities of essential oil derived from the wild rhizome of Atractylodes macrocephala Koidz","volume":"17","author":"Wu","year":"2020","journal-title":"Chem. Biodivers."},{"key":"ref_22","first-page":"725","article-title":"Simultaneous determination of atractylone, hinesol, \u03b2-eudesmol, atractylodin in Atractylodes lancea and hierarchical cluster analysis","volume":"35","author":"Zhang","year":"2010","journal-title":"Zhongguo Zhong Yao Za Zhi"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nri2653","article-title":"Intestinal mucosal barrier function in health and disease","volume":"9","author":"Turner","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nri.2016.88","article-title":"Immunological aspects of intestinal mucus and mucins","volume":"16","author":"Johansson","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7204","DOI":"10.18632\/oncotarget.23208","article-title":"Inflammatory responses and inflammation-associated diseases in organs","volume":"9","author":"Chen","year":"2018","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.bbamcr.2018.02.010","article-title":"Nrf2-Keap1 signaling in oxidative and reductive stress","volume":"1865","author":"Bellezza","year":"2018","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mishra, B., and Jha, R. (2019). Oxidative stress in the poultry gut: Potential challenges and interventions. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00060"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.tim.2017.05.004","article-title":"Gut microbiota dysbiosis in post-weaning piglets: Understanding the keys to health","volume":"25","author":"Gresse","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/1574-6968.12608","article-title":"The chicken gastrointestinal microbiome","volume":"360","author":"Oakley","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.cell.2016.05.041","article-title":"From dietary fiber to host physiology: Short-chain fatty acids as key bacterial metabolites","volume":"165","author":"Koh","year":"2016","journal-title":"Cell"},{"key":"ref_31","first-page":"2373","article-title":"Effects of Rhizoma atractylodis oil on rumen fermentation and nutrient degradability in vitro in Jinjiang yellow cattle","volume":"26","author":"Xu","year":"2014","journal-title":"Chin. J. Anim. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.aninu.2017.12.004","article-title":"Gastrointestinal tract (gut) health in the young pig","volume":"4","author":"Pluske","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.livsci.2011.06.013","article-title":"Dietary supplementation with Atractylodes macrocephala Koidz polysaccharides ameliorate metabolic status and improve immune function in early-weaned pigs","volume":"142","author":"Li","year":"2011","journal-title":"Livest. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wang, X., Wang, Y., Mao, Y., Hu, A., Xu, T., Yang, Y., Wang, F., Zhou, G., Guo, X., and Cao, H. (2022). The beneficial effects of traditional Chinese medicine on antioxidative status and inflammatory cytokines expression in the liver of piglets. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.937745"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/ame2.12104","article-title":"Chinese herb feed additives improved the growth performance, meat quality, and nutrient digestibility parameters of pigs","volume":"3","author":"Lin","year":"2020","journal-title":"Anim. Model. Exp. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/2049-1891-4-51","article-title":"Effects of Chinese herbal medicine on plasma glucose, protein, and energy metabolism in sheep","volume":"4","author":"Liang","year":"2013","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"613","DOI":"10.3390\/ruminants4040042","article-title":"Supplementing forage with traditional Chinese medicine can increase microbial protein synthesis in sheep","volume":"4","author":"Liang","year":"2024","journal-title":"Ruminants"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Liu, B., Ma, R., Yang, Q., Yang, Y., Fang, Y., and Sun, Z. (2023). Effects of traditional Chinese herbal feed additive on production performance, egg quality, antioxidant capacity, immunity and intestinal health of laying hens. Animals, 13.","DOI":"10.3390\/ani13152510"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Sun, Y., Zhang, M., Shi, D., Dai, X., and Li, X. (2024). Effects of designed herbal formula on growth performance, blood indices, organ traits, and cecum microbiology in broilers. Vet. Sci., 11.","DOI":"10.3390\/vetsci11030107"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"103171","DOI":"10.1016\/j.psj.2023.103171","article-title":"Immunomodulatory effect of Atractylodis macrocephala Koidz. polysaccharides in vitro","volume":"103","author":"Hu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.2527\/jas.2014-8754","article-title":"Ruminant Nutrition Symposium: How to use data on the rumen microbiome to improve our understanding of ruminant nutrition","volume":"93","author":"Firkins","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_42","unstructured":"Gerber, P.J., Steinfeld, H., Henderson, B., Mottet, A., Opio, C., Dijkman, J., Falcucci, A., and Branca, G. (2013). Tackling Climate Change Through Livestock: A Global Assessment of Emissions and Mitigation Opportunities, FAO."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2789\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:19:18Z","timestamp":1788538758000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/17\/2789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":42,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16172789"],"URL":"https:\/\/doi.org\/10.3390\/ani16172789","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}