{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:11:37Z","timestamp":1788775897887,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Project for Natural Science Research in Anhui Higher Education Institutions","award":["2024AH040060"],"award-info":[{"award-number":["2024AH040060"]}]},{"DOI":"10.13039\/501100010816","name":"Anhui Provincial Major Science and Technology Project","doi-asserted-by":"publisher","award":["202513b10050011"],"award-info":[{"award-number":["202513b10050011"]}],"id":[{"id":"10.13039\/501100010816","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010814","name":"Anhui Provincial Programme for Science and Engineering Lecturers from Higher Education Institutions to Undertake Work Placements in Enterprises","doi-asserted-by":"publisher","award":["2025jsqygz44"],"award-info":[{"award-number":["2025jsqygz44"]}],"id":[{"id":"10.13039\/501100010814","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Cyclic heat stress compromises egg production and antioxidant defenses in laying hens. The present study employed a short-term, 14-day cyclic heat-stress model to evaluate whether dietary supplementation with a chlorogenic acid-rich green coffee bean extract could mitigate these effects. Before initiation of the heat-stress experiment, all hens were maintained under thermoneutral conditions and received the basal diet without the extract. Following 1 week of adaptation and 4 weeks of thermoneutral feeding, 240 approximately 50-week-old Jingfen No. 8 hens were allocated to a thermoneutral control, a heat-stress control, or heat-stressed groups receiving 100 or 200 mg\/kg extract containing 70% chlorogenic acid, with four independent replicates of 15 hens each (60 hens per treatment). Heat-stressed hens were subjected to a 14-day cyclic heat-stress challenge at 32\u201335 \u00b0C for 9 h\/day. Heat stress decreased average egg weight and laying rate, increased serum malondialdehyde, and reduced total antioxidant capacity (p &lt; 0.05). Both supplemented groups improved average egg weight and laying rate, reduced malondialdehyde and total cholesterol, and increased total antioxidant capacity relative to heat-stressed controls (p &lt; 0.05). Supplementation altered hepatic expression of genes associated with proliferation, apoptosis, and autophagy. Exploratory metabolomics detected changes in the 100 mg\/kg group but not the 200 mg\/kg group relative to heat-stressed controls. The extract partially mitigated production and antioxidant impairments during cyclic heat stress.<\/jats:p>","DOI":"10.3390\/ani16172801","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T09:23:37Z","timestamp":1788773017000},"page":"2801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Chlorogenic Acid-Rich Green Coffee Bean Extract Improves Laying Performance and Antioxidant Status in Heat-Stressed Laying Hens"],"prefix":"10.3390","volume":"16","author":[{"given":"Yuqing","family":"Mu","sequence":"first","affiliation":[{"name":"College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Xuezheng","family":"Shang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xuezhuang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.5713\/ab.24.0675","article-title":"Invited Review\u2014Challenges and Constraints in the Sustainability of Poultry Farming in Japan","volume":"38","author":"Ohtsu","year":"2025","journal-title":"Anim. 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