{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:08:38Z","timestamp":1789117718910,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472888"],"award-info":[{"award-number":["32472888"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32502882"],"award-info":[{"award-number":["32502882"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32472888"],"award-info":[{"award-number":["32472888"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32502882"],"award-info":[{"award-number":["32502882"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Sichuan Science and Technology Program","award":["2026YFHZ0291"],"award-info":[{"award-number":["2026YFHZ0291"]}]},{"name":"Technology Projects of Xizang Autonomous Region","award":["XZ202501ZY0147"],"award-info":[{"award-number":["XZ202501ZY0147"]}]},{"name":"Fundamental Research Funds for the Central Universities of Southwest Minzu University","award":["ZYN2024197"],"award-info":[{"award-number":["ZYN2024197"]}]},{"award":["ZYN2024197"],"award-info":[{"award-number":["ZYN2024197"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Program for Pig Industry Technology System Innovation Team of Sichuan Province","award":["sccxtd-2026-08"],"award-info":[{"award-number":["sccxtd-2026-08"]}]},{"name":"Ganzi Prefecture \u201cAcademy-Prefecture\u201d Agricultural Science and Technology Cooperation Project","award":["2026"],"award-info":[{"award-number":["2026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Tibetan pigs exhibit remarkable adaptability to hypoxic high-altitude conditions. However, metatranscriptomics has not been used to investigate the transcriptional activity and functional responses of the gastrointestinal microbiota as part of their adaptation to high altitude. We collected 57 samples of the contents of the stomach, jejunum, cecum, colon, and rectum of six high-altitude Tibetan pigs (n = 6, elevation 3750 m) and six low-altitude black pigs (n = 6, elevation 500 m) for metatranscriptomic analysis. We identified 79 differentially abundant bacterial genera and 69 differentially abundant bacterial species. In particular, Lactobacillus crispatus and Lactobacillus delbrueckii were significantly more abundant in the gastrointestinal microbiota of the high-altitude Tibetan pigs than in that of the low-altitude black pigs. Functional analysis revealed differences in transcripts associated with energy, carbohydrate, and amino acid metabolism. The microbial correlation network of Tibetan pigs contained more nodes and edges than that of black pigs. Consistent with this, within the CAZyme database, the CBM32 family of carbohydrate-binding modules and GH27 glycosidases were significantly enriched in the Tibetan pig samples, suggesting that these enzymes may play a crucial role in energy acquisition and carbon source metabolism in these pigs. These findings provide insights into the gastrointestinal microbial characteristics of Tibetan pigs and black pigs. In summary, these changes may enhance the stability of the gut ecosystem of Tibetan pigs and improve their energy utilization in nutrient-deficient high-altitude environments. These findings suggest that the gut microbiota may contribute to metabolic regulation in Tibetan pigs at high altitude and provide important clues regarding the gastrointestinal adaptations of high-altitude animals.<\/jats:p>","DOI":"10.3390\/ani16182861","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:17:42Z","timestamp":1789114662000},"page":"2861","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metatranscriptomic Analysis Reveals Functional Features of the Gastrointestinal Microbiota in Tibetan Pigs"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9081-0679","authenticated-orcid":false,"given":"Zhiyuan","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8741-8051","authenticated-orcid":false,"given":"Xuanlin","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Yan","family":"Liang","sequence":"additional","affiliation":[{"name":"Animal Genetic Breeding and Reproduction Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Xuan","family":"Tao","sequence":"additional","affiliation":[{"name":"Animal Genetic Breeding and Reproduction Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Bo","family":"Ran","sequence":"additional","affiliation":[{"name":"Animal Genetic Breeding and Reproduction Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Pengliang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Yiren","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ai, H., Yang, B., Li, J., Xie, X., Chen, H., and Ren, J. (2014). Population history and genomic signatures for high-altitude adaptation in Tibetan pigs. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-834"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1038\/ng.2811","article-title":"Genomic analyses identify distinct patterns of selection in domesticated pigs and Tibetan wild boars","volume":"45","author":"Li","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/eva.12887","article-title":"The fine-scale genetic structure and selection signals of Chinese indigenous pigs","volume":"13","author":"Huang","year":"2020","journal-title":"Evol. Appl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/vms3.639","article-title":"Vascular characteristics and expression of hypoxia genes in Tibetan pigs\u2019 hearts","volume":"8","author":"Yang","year":"2022","journal-title":"Vet. Med. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"283","DOI":"10.5194\/aab-64-283-2021","article-title":"Characteristics of Tibetan pig lung tissue in response to a hypoxic environment on the Qinghai-Tibet Plateau","volume":"64","author":"Yang","year":"2021","journal-title":"Arch. Anim. Breed."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/wsbm.19","article-title":"Gastrointestinal system","volume":"2","author":"Cheng","year":"2010","journal-title":"WIREs Syst. Biol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/j.suc.2011.06.010","article-title":"Gastric acid and digestive physiology","volume":"91","author":"Ramsay","year":"2011","journal-title":"Surg. Clin. N. Am."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/s40104-018-0270-0","article-title":"The role of dietary fibre in pig production, with a particular emphasis on reproduction","volume":"9","author":"Jarrett","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"671","DOI":"10.5187\/jast.2022.e58","article-title":"Gut microbiome-produced metabolites in pigs: A review on their biological functions and the influence of probiotics","volume":"64","author":"Vasquez","year":"2022","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s42523-025-00419-7","article-title":"Unraveling the composition and function of pig gut microbiome from metagenomics","volume":"7","author":"Tang","year":"2025","journal-title":"Anim. Microbiome"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.hlife.2024.11.004","article-title":"Altitude adaptation: The unseen work of gut microbiota","volume":"3","author":"Guo","year":"2025","journal-title":"hLife"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.bbrc.2019.05.085","article-title":"Gut microbiota adaptation to high altitude in indigenous animals","volume":"516","author":"Ma","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e6","DOI":"10.1002\/imt2.6","article-title":"Gut microbiota insights into human adaption to high-plateau diet","volume":"1","author":"Huang","year":"2022","journal-title":"iMeta"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bai, X., Lu, S., Yang, J., Jin, D., Pu, J., D\u00edaz Moy\u00e1, S., Xiong, Y., Rossello-Mora, R., and Xu, J. (2018). Precise Fecal Microbiome of the Herbivorous Tibetan Antelope Inhabiting High-Altitude Alpine Plateau. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02321"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1146\/annurev-biodatasci-031121-103035","article-title":"Metatranscriptomics for the Human Microbiome and Microbial Community Functional Profiling","volume":"4","author":"Zhang","year":"2021","journal-title":"Annu. Rev. Biomed. Data Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1016\/j.chom.2025.06.004","article-title":"Metatranscriptomics catches gut microbes in the act","volume":"33","author":"Liu","year":"2025","journal-title":"Cell Host Microbe"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s42523-021-00108-1","article-title":"Metatranscriptomic analysis of colonic microbiota\u2019s functional response to different dietary fibers in growing pigs","volume":"3","author":"Xu","year":"2021","journal-title":"Anim. Microbiome"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e0254925","DOI":"10.1128\/spectrum.02549-25","article-title":"Effect of altitudes on serum parameters, metabolome, and gut microbiota in yaks on the Qinghai-Tibet Plateau","volume":"14","author":"Xie","year":"2026","journal-title":"Microbiol. Spectr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sha, Y., Ren, Y., Zhao, S., He, Y., Guo, X., Pu, X., Li, W., Liu, X., Wang, J., and Li, S. (2022). Response of Ruminal Microbiota-Host Gene Interaction to High-Altitude Environments in Tibetan Sheep. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012430"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"E2329","DOI":"10.1073\/pnas.1319284111","article-title":"Relating the metatranscriptome and metagenome of the human gut","volume":"111","author":"Franzosa","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1609\/icwsm.v3i1.13937","article-title":"Gephi: An Open Source Software for Exploring and Manipulating Networks","volume":"Volume 3","author":"Bastian","year":"2009","journal-title":"Proceedings of the International AAAI Conference on Web and Social Media"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1097\/MCG.0b013e31823711fd","article-title":"The intestinal microbiota and obesity","volume":"46","author":"Kallus","year":"2012","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1111\/1758-2229.12980","article-title":"Polysaccharide degradation by the Bacteroidetes: Mechanisms and nomenclature","volume":"13","author":"McKee","year":"2021","journal-title":"Environ. Microbiol. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"289","DOI":"10.4161\/gmic.19897","article-title":"Microbial degradation of complex carbohydrates in the gut","volume":"3","author":"Flint","year":"2012","journal-title":"Gut Microbes"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bai, X., Gu, Y., Li, D., and Li, M. (2025). Gut Metagenome Reveals the Microbiome Signatures in Tibetan and Black Pigs. Animals, 15.","DOI":"10.3390\/ani15050753"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/1462-2920.13589","article-title":"Formation of propionate and butyrate by the human colonic microbiota","volume":"19","author":"Louis","year":"2017","journal-title":"Environ. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.celrep.2018.12.028","article-title":"Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids","volume":"26","author":"Wang","year":"2019","journal-title":"Cell Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1186\/s13059-024-03373-w","article-title":"Gut microbiota contributes to high-altitude hypoxia acclimatization of human populations","volume":"25","author":"Su","year":"2024","journal-title":"Genome Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1038\/s41396-023-01521-x","article-title":"Land use conversion increases network complexity and stability of soil microbial communities in a temperate grassland","volume":"17","author":"Cornell","year":"2023","journal-title":"ISME J."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wang, X., Zhang, Q., Wang, W., Wang, X., Song, B., Li, J., Cui, W., Jiang, Y., Xie, W., and Tang, L. (2025). Multi-Strain Probiotic Regulates the Intestinal Mucosal Immunity and Enhances the Protection of Piglets Against Porcine Epidemic Diarrhea Virus Challenge. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081738"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Jang, H., Joung, H., Chu, J., Cho, M., Kim, Y.W., Kim, K.H., Shin, C.H., Lee, J., and Ha, J.H. (2024). Lactobacillus delbrueckii subsp. lactis CKDB001 Ameliorates Metabolic Complications in High-Fat Diet-Induced Obese Mice. Nutrients, 16.","DOI":"10.3390\/nu16244260"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e14862","DOI":"10.1002\/advs.202514862","article-title":"The High-Altitude Adaptation Characteristics of Microbiota-Host Cross-Talk in Yak Gastrointestinal Track","volume":"13","author":"Huang","year":"2026","journal-title":"Adv. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wang, J., Liu, J., Guo, T., Zheng, C., Wang, F., Liu, T., Yuan, C., and Lu, Z. (2025). Multi-omics insights into key microorganisms and metabolites in Tibetan sheep\u2019s high-altitude adaptation. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1616555"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"126447","DOI":"10.1016\/j.micres.2020.126447","article-title":"Gut microbiota of Tibetans and Tibetan pigs varies between high and low altitude environments","volume":"235","author":"Zeng","year":"2020","journal-title":"Microbiol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.phrs.2012.11.005","article-title":"Intestinal luminal nitrogen metabolism: Role of the gut microbiota and consequences for the host","volume":"68","author":"Davila","year":"2013","journal-title":"Pharmacol. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ran, B., Tang, J., Wang, Y., Tao, X., Yang, Y., Yang, X., Gong, J., He, Z., Gu, Y., and Liu, P. (2025). Transcriptional insights into gastrointestinal adaptations in pigs to high altitude. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1723710"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Niu, H., Feng, X.Z., Shi, C.W., Zhang, D., Chen, H.L., Huang, H.B., Jiang, Y.L., Wang, J.Z., Cao, X., and Wang, N. (2022). Gut Bacterial Composition and Functional Potential of Tibetan Pigs Under Semi-Grazing. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.850687"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1016\/j.jmb.2013.11.029","article-title":"An unusual mode of galactose recognition by a family 32 carbohydrate-binding module","volume":"426","author":"Grondin","year":"2014","journal-title":"J. Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ficko-Blean, E., Stuart, C.P., Suits, M.D., Cid, M., Tessier, M., Woods, R.J., and Boraston, A.B. (2012). Carbohydrate recognition by an architecturally complex \u03b1-N-acetylglucosaminidase from Clostridium perfringens. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0033524"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Liu, H., Hu, L., Han, X., Zhao, N., Xu, T., Ma, L., Wang, X., Zhang, X., Kang, S., and Zhao, X. (2020). Tibetan Sheep Adapt to Plant Phenology in Alpine Meadows by Changing Rumen Microbial Community Structure and Function. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.587558"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hu, B., Wang, J., Li, Y., Ge, J., Pan, J., Li, G., He, Y., Zhong, H., Wang, B., and Huang, Y. (2023). Gut microbiota facilitates adaptation of the plateau zokor (Myospalax baileyi) to the plateau living environment. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1136845"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/j.tim.2017.06.009","article-title":"Navigating the Gut Buffet: Control of Polysaccharide Utilization in Bacteroides spp.","volume":"25","author":"Schwalm","year":"2017","journal-title":"Trends Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Heinritz, S.N., Weiss, E., Eklund, M., Aumiller, T., Louis, S., Rings, A., Messner, S., Camarinha-Silva, A., Seifert, J., and Bischoff, S.C. (2016). Intestinal Microbiota and Microbial Metabolites Are Changed in a Pig Model Fed a High-Fat\/Low-Fiber or a Low-Fat\/High-Fiber Diet. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0154329"},{"key":"ref_44","first-page":"189","article-title":"Gut microbiota composition in Himalayan and Andean populations and its relationship with diet, lifestyle and adaptation to the high-altitude environment","volume":"96","author":"Quagliariello","year":"2019","journal-title":"J. Anthropol. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Cao, L., and Zhu, W. (2026). Insights from the high-altitude animal gut adaptation model: Mechanisms of obesity regulation via microbiota-derived metabolite homeostasis and the gut-X axis. Front. Microbiol., 17.","DOI":"10.3389\/fmicb.2026.1795452"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/nrmicro3552","article-title":"Gut biogeography of the bacterial microbiota","volume":"14","author":"Donaldson","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gresse, R., Chaucheyras Durand, F., Duni\u00e8re, L., Blanquet-Diot, S., and Forano, E. (2019). Microbiota Composition and Functional Profiling Throughout the Gastrointestinal Tract of Commercial Weaning Piglets. Microorganisms, 7.","DOI":"10.3390\/microorganisms7090343"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2861\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:41:50Z","timestamp":1789116110000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":47,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16182861"],"URL":"https:\/\/doi.org\/10.3390\/ani16182861","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}