{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:10:07Z","timestamp":1789380607771,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004035","name":"Jordan University of Science and Technology","doi-asserted-by":"publisher","award":["275\/2026"],"award-info":[{"award-number":["275\/2026"]}],"id":[{"id":"10.13039\/501100004035","id-type":"DOI","asserted-by":"publisher"}]},{"award":["275\/2026"],"award-info":[{"award-number":["275\/2026"]}],"id":[{"id":"https:\/\/ror.org\/03y8mtb59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Using industrial waste in livestock feed is crucial for lowering production costs while reducing the environmental impact. Accordingly, the goal of this study was to evaluate the effects of feeding coffee pulp (COP) on the Awassi ewes\u2019 milk production, nutrient digestibility, and blood parameters. Thirty 5-year-old Awassi ewes with an initial body weight of 56.7 \u00b1 3.72 kg were selected for the experiment. Three dietary rations were used, and ten ewes were randomly assigned to each diet for nine weeks, including one week of adaptation. The diets were: (1) 0 COP (CON), (2) 100 COP (COP100), and (3) 200 g\/kg COP of dietary dry matter (COP200). Coffee pulp was included at 100 and 200 g\/kg of dietary DM; as a result, barley grain increased whereas soybean meal and wheat straw decreased as COP inclusion increased, while the diets remained approximately isonitrogenous. We evaluated effects on intake, performance, milk production and composition, digestibility, nitrogen balance, and selected serum biochemical parameters. Nutrient intake did not differ among dietary groups (p &gt; 0.05). Milk lactose concentration was significantly higher in COP100 and COP200 than in CON (p = 0.015). No adverse changes were detected in the measured productive responses or selected serum biochemical parameters. The cost of milk production decreased with coffee pulp inclusion, from approximately US$ 0.359\/kg milk in CON to US$0.325 and US$ 0.300\/kg milk in COP100 and COP200, respectively (p = 0.002), corresponding to reductions of approximately 9.5% and 16.4%. Blood urea nitrogen was higher (p = 0.017) in ewes that consumed the COP diets than in those that consumed the CON diet. These results indicated that substituting conventional feedstuffs in ewes\u2019 diets with up to 200 g\/kg of COP was effective and reduced milk production costs.<\/jats:p>","DOI":"10.3390\/ani16182879","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:34:01Z","timestamp":1789378441000},"page":"2879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the Impact of Coffee Pulp on Milk Yield and Blood Parameters in Awassi Ewes"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0315-4032","authenticated-orcid":false,"given":"Belal S.","family":"Obeidat","sequence":"first","affiliation":[{"name":"Department of Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Hadil S.","family":"Subih","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Alyahya","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, Universiti Sains Malaysia, Gelugor 11800, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116688","DOI":"10.1016\/j.anifeedsci.2026.116688","article-title":"Effects of coffee pulp in the diets of growing lambs on growth, meat quality, and blood parameters","volume":"336","author":"Obeidat","year":"2026","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"114471","DOI":"10.1016\/j.anifeedsci.2020.114471","article-title":"Performance, carcass traits and meat quality of lambs fed coffee hulls treated with calcium oxide","volume":"264","author":"Nunes","year":"2020","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.13057\/biodiv\/d240745","article-title":"Evaluation of nutritive values of various non-conventional protein sources as potential feed ingredients for ruminants","volume":"24","author":"Samadi","year":"2023","journal-title":"Biodiversitas J. Biol. Divers."},{"key":"ref_4","first-page":"1465","article-title":"Methane production from coffee pulp by microorganism of rumen fluid and cow dung in co-digestion","volume":"56","author":"Widjaja","year":"2017","journal-title":"Chem. Eng. Trans."},{"key":"ref_5","first-page":"15","article-title":"Cultivation of pleurotus mushrooms on Brazilian coffee husk and effects of caffeine and tannic acid","volume":"15","author":"Fan","year":"2003","journal-title":"Micol. Apl. Int."},{"key":"ref_6","first-page":"189","article-title":"Drying of coffee pulp: Process parameters, mathematical model and its effect over physicochemical properties","volume":"30","author":"Torres","year":"2019","journal-title":"Inf. Tecnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11250-023-03451-4","article-title":"Effects of the inclusion of coffee pulp silage in the diet on the performance and profitability of crossbred milk cows in the middle tropics","volume":"55","year":"2023","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4067\/S0719-81322018000200095","article-title":"Health status and productivity of sheep fed coffee pulp during fattening","volume":"50","year":"2018","journal-title":"Austral. J. Vet. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.smallrumres.2014.09.008","article-title":"Productive performance and oxidative status of sheep fed diets supplemented with coffee pulp","volume":"123","year":"2015","journal-title":"Small Rumin. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"114461","DOI":"10.1016\/j.anifeedsci.2020.114461","article-title":"Valorisation of spent coffee grounds as functional feed ingredient improves productive performance of Latxa dairy ewes","volume":"264","author":"Ruiz","year":"2020","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_11","first-page":"589","article-title":"Spent coffee ground as second-generation feedstuff for dairy cattle","volume":"11","author":"Orive","year":"2020","journal-title":"Biomass Conver. Biorefin."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100501","DOI":"10.1016\/j.animal.2022.100501","article-title":"Effects of spent coffee grounds on production traits, haematological parameters, and antioxidant activity of blood and milk in dairy goat","volume":"16","author":"Carta","year":"2022","journal-title":"Animal"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.5713\/ajas.18.0846","article-title":"Effect of fermented spent instant coffee grounds on milk productivity and blood profiles of lactating dairy cows","volume":"32","author":"Choi","year":"2019","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_14","unstructured":"NRC (2007). Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervide, and New World Camelids, National Academy of Sciences."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1007\/s11250-026-04873-6","article-title":"The effects of sesame oil supplementation on the performance of Awassi ewes and their offspring","volume":"58","author":"Obeidat","year":"2026","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0921-4488(91)90137-F","article-title":"Effects of feeding calcium salts on long chain fatty acids on milk yield, milk composition and plasma parameters of lactating goats","volume":"6","author":"Baldi","year":"1992","journal-title":"Small Rumin. Res."},{"key":"ref_17","unstructured":"AOAC (1990). Official Methods of Analysis, Association Analytical Chemist. [15th ed.]."},{"key":"ref_18","unstructured":"SAS Institute Inc. (2013). SAS\/STAT Users Guide, SAS Institute Inc.. Version 9.4."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Maxiselly, Y., Chiarawipa, R., Somnuk, K., Hamchara, P., Cherdthong, A., Suntara, C., Prachumchai, R., and Chanjula, P. (2022). Digestibility, blood parameters, rumen fermentation, hematology, and nitrogen balance of goats after receiving supplemental coffee cherry pulp as a source of phytochemical nutrients. Vet. Sci., 9.","DOI":"10.3390\/vetsci9100532"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Estrada-Flores, J.G., Pedraza-Beltr\u00e1n, P.E., Yong-\u00c1ngel, G., Avil\u00e9s-Nova, F., Rayas-Amor, A.A., Sol\u00eds-M\u00e9ndez, A.D., Gonz\u00e1lez-Ronquillo, M., V\u00e1zquez-Carrillo, M.F., and Castel\u00e1n-Ortega, O.A. (2021). Effect of increasing supplementation levels of coffee pulp on milk yield and food intake in dual-purpose cows: An alternative feed byproduct for smallholder dairy systems of tropical climate regions. Agriculture, 11.","DOI":"10.3390\/agriculture11050416"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1080\/1828051X.2019.1579680","article-title":"Effects of alternative feedstuffs on growth performance, carcass characteristics, and meat quality of growing Awassi lambs","volume":"18","author":"Awawdeh","year":"2019","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1590\/S1516-35982010000500027","article-title":"Coffee hull in the diet of dairy heifers: Nitrogen balance and microbial protein synthesis","volume":"39","author":"Garcia","year":"2010","journal-title":"Rev. Bras. Zootec."},{"key":"ref_23","first-page":"2170","article-title":"Coffee hulls in the diet of sheep: Intake and apparent digestibility","volume":"33","author":"Garcia","year":"2004","journal-title":"Rev. Bras. Zootec."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"363","DOI":"10.5713\/ajas.17.0654","article-title":"Effects of dietary fermented spent coffee ground on nutrient digestibility and nitrogen utilization in sheep","volume":"31","author":"Choi","year":"2018","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"14","DOI":"10.2527\/2006.8413_supplE14x","article-title":"Sites, rates, and limits of starch digestion and glucose metabolism in growing cattle","volume":"84","author":"Huntington","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/s11250-009-9491-8","article-title":"Feed intake, digestibility, nitrogen utilization, and body weight change of sheep consuming wheat straw supplemented with local agricultural and agro-industrial by-Products","volume":"42","author":"Nurfeta","year":"2009","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"20220381","DOI":"10.1515\/opag-2022-0381","article-title":"Evaluation of coffee pulp as a feed ingredient for ruminants: A meta-analysis","volume":"9","author":"Fhonna","year":"2024","journal-title":"Open Agric."},{"key":"ref_28","unstructured":"Kaneko, J.J. (2014). Clinical Biochemistry of Domestic Animals, Academic Press. [3rd ed]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3628","DOI":"10.3168\/jds.2016-11794","article-title":"Effects of rumen undegradable protein supplementation on productive performance and indicators of protein and energy metabolism in Holstein fresh cows","volume":"100","author":"Amanlou","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1111\/asj.13147","article-title":"Effects of Antidesma thwaitesianum Muell. Arg. pomace as a source of plant secondary compounds on digestibility, rumen environment, hematology, and milk production in dairy cows","volume":"90","author":"Gunun","year":"2019","journal-title":"Anim. Sci. J."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2879\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:56:40Z","timestamp":1789379800000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,13]]},"references-count":30,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16182879"],"URL":"https:\/\/doi.org\/10.3390\/ani16182879","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,13]]}}}