{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:28:54Z","timestamp":1789658934600,"version":"4.0.1"},"reference-count":86,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for the Central Universities, Southwest Minzu University","award":["ZYN2025039"],"award-info":[{"award-number":["ZYN2025039"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"crossref"}]},{"award":["ZYN2025039"],"award-info":[{"award-number":["ZYN2025039"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Key Research and Development Program sub-project","award":["2023YFD180250202"],"award-info":[{"award-number":["2023YFD180250202"]}]},{"name":"Innovation Team Development Funds for Sichuan Mutton Goat & Sheep, China","award":["SCCXTD-2024-14"],"award-info":[{"award-number":["SCCXTD-2024-14"]}]},{"name":"Scientific and Technological Innovation Team for Qinghai-Tibetan Plateau Research in Southwest Minzu University","award":["2024CXTD08"],"award-info":[{"award-number":["2024CXTD08"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2024CXTD08"],"award-info":[{"award-number":["2024CXTD08"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Bovine Respiratory Disease (BRD) is a leading cause of economic losses in the global cattle industry, with Mannheimia haemolytica and Pasteurella multocida as the primary bacterial pathogens. This review provides a comprehensive overview of vaccine research progress against these two pathogens, covering inactivated vaccines, live attenuated vaccines, subunit vaccines, DNA vaccines, recombinant vector vaccines, and outer membrane vesicle vaccines. Their immune mechanisms, protective efficacy, adjuvant applications, and field performance are discussed. Key challenges include maternal antibody interference, the increasing prevalence of M. haemolytica serotype A6 that evades current commercial vaccines, and the serotype diversity of P. multocida. Future vaccine development should focus on overcoming maternal antibody interference, enhancing cross-serotype protection, optimizing mucosal adjuvants and delivery systems, and developing multivalent vaccines covering major epidemic serotypes. Integrating reverse vaccinology, multi-epitope vaccine design, and nanotechnology holds promise for safer, more effective, and broader-spectrum next-generation vaccines.<\/jats:p>","DOI":"10.3390\/ani16182911","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:58:59Z","timestamp":1789545539000},"page":"2911","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pasteurella multocida and Mannheimia haemolytica Vaccines for Bovine Respiratory Disease: A Comprehensive Review"],"prefix":"10.3390","volume":"16","author":[{"given":"Minyi","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0194-4604","authenticated-orcid":false,"given":"Guangfu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibing","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"O\u2019Donoghue, S., Waters, S.M., Morris, D.W., and Earley, B. (2025). A Comprehensive Review: Bovine Respiratory Disease, Current Insights into Epidemiology, Diagnostic Challenges, and Vaccination. Vet. Sci., 12.","DOI":"10.3390\/vetsci12080778"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.2527\/jas.2006-046","article-title":"Bovine respiratory disease in feedlot cattle: Environmental, genetic, and economic factors","volume":"84","author":"Snowder","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1017\/S1466252318000142","article-title":"Mannheimia haemolytica in bovine respiratory disease: Immunogens, potential immunogens, and vaccines","volume":"19","author":"Confer","year":"2018","journal-title":"Anim. Health Res. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1017\/S1466252309990193","article-title":"Update on bacterial pathogenesis in BRD","volume":"10","author":"Confer","year":"2009","journal-title":"Anim. Health Res. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CMR.00024-13","article-title":"Pasteurella multocida: From zoonosis to cellular microbiology","volume":"26","author":"Wilson","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2006.00442.x","article-title":"Pasteurella multocida pathogenesis: 125 years after Pasteur","volume":"265","author":"Harper","year":"2006","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1292\/jvms.19-0256","article-title":"Effect of combined vaccination against Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni on the prevention of respiratory diseases in young Japanese Black calves in the field","volume":"81","author":"Nagai","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_8","first-page":"1095","article-title":"The epidemiology of bovine respiratory disease: What is the evidence for predisposing factors?","volume":"51","author":"Taylor","year":"2010","journal-title":"Can. Vet. J."},{"key":"ref_9","first-page":"724","article-title":"Antimicrobial resistance in Mannheimia haemolytica and Pasteurella multocida from bovine respiratory disease","volume":"24","author":"Portis","year":"2012","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s11259-025-10943-1","article-title":"Prevalence and mechanisms of antimicrobial resistance in respiratory bacterial pathogens of ruminants: A systematic review","volume":"50","author":"Peter","year":"2025","journal-title":"Vet. Res. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1356208","DOI":"10.3389\/fmicb.2024.1356208","article-title":"Macrolide resistance in Mannheimia haemolytica isolates associated with bovine respiratory disease from the German national resistance monitoring program GERM-Vet 2009 to 2020","volume":"15","author":"Kostova","year":"2024","journal-title":"Front. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e0190924","DOI":"10.1128\/aem.01909-24","article-title":"Retrospective analysis of antimicrobial resistance associated with bovine respiratory disease","volume":"91","author":"Kos","year":"2025","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1128\/microbiolspec.ARBA-0009-2017","article-title":"Antimicrobial resistance: A One Health perspective","volume":"6","author":"McEwen","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e00158","DOI":"10.1016\/j.heliyon.2016.e00158","article-title":"Identification of leukotoxin and other vaccine candidate proteins in a Mannheimia haemolytica commercial antigen","volume":"2","author":"Tucci","year":"2016","journal-title":"Heliyon"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1079\/AHRR200242","article-title":"Role of Mannheimia haemolytica leukotoxin in the pathogenesis of bovine pneumonic pasteurellosis","volume":"3","author":"Jeyaseelan","year":"2002","journal-title":"Anim. Health Res. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1553396","DOI":"10.3389\/fvets.2025.1553396","article-title":"Immunogenicity and protective efficacy of seven candidate vaccines boosted with recombinant proteins, whole-cell bacterins of three serotypes of Mannheimia haemolytica, and an emulsion-type adjuvant","volume":"12","author":"Balevi","year":"2025","journal-title":"Front. Vet. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s11259-023-10207-w","article-title":"Detection of immune effects of the Mannheimia haemolytica gamma irradiated vaccine in sheep","volume":"48","author":"Ahmed","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1016\/S0264-410X(00)00498-9","article-title":"Adjuvants designed for veterinary and human vaccines","volume":"19","author":"Aucouturier","year":"2001","journal-title":"Vaccine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1038\/nm.3409","article-title":"Key roles of adjuvants in modern vaccines","volume":"19","author":"Reed","year":"2013","journal-title":"Nat. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.vetimm.2009.04.018","article-title":"Intranasal vaccination of young Holstein calves with Mannheimia haemolytica chimeric protein PlpE-LKT (SAC89) and cholera toxin","volume":"132","author":"Confer","year":"2009","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1078\/1438-4221-00200","article-title":"RTX toxins in Pasteurellaceae","volume":"292","author":"Frey","year":"2002","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"535","DOI":"10.2478\/jvetres-2025-0056","article-title":"Protective efficacy of the recombinantly expressed C-terminal domain of Mannheimia haemolytica leukotoxin in mice and goats","volume":"69","author":"Doan","year":"2025","journal-title":"J. Vet. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1128\/IAI.72.12.7265-7274.2004","article-title":"Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE","volume":"72","author":"Ayalew","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1016\/S0264-410X(03)00213-5","article-title":"Immunogenicity of recombinant Mannheimia haemolytica serotype 1 outer membrane protein PlpE and augmentation of a commercial vaccine","volume":"21","author":"Confer","year":"2003","journal-title":"Vaccine"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetmic.2012.08.019","article-title":"The OmpA family of proteins: Roles in bacterial pathogenesis and immunity","volume":"163","author":"Confer","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_26","first-page":"672","article-title":"Molecular studies of Ssa1, a serotype-specific antigen of Mannheimia haemolytica","volume":"69","author":"Lo","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1251046","DOI":"10.3389\/fpls.2023.1251046","article-title":"Development of a plant-based oral vaccine candidate against the bovine respiratory pathogen Mannheimia haemolytica","volume":"14","author":"Kaldis","year":"2023","journal-title":"Front. Plant Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/pbi.12471","article-title":"Plant-made oral vaccines against human infectious diseases-Are we there yet?","volume":"13","author":"Chan","year":"2015","journal-title":"Plant Biotechnol. J."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Uddin, M.S., Kaldis, A., Menassa, R., Guluarte, J.O., Barreda, D.R., Guan, L.L., and Alexander, T.W. (2024). Mucosal Immunization with Spore-Based Vaccines against Mannheimia haemolytica Enhances Antigen-Specific Immunity. Vaccines, 12.","DOI":"10.3390\/vaccines12040375"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"12981","DOI":"10.1038\/s41598-023-29732-4","article-title":"Development of a spore-based mucosal vaccine against the bovine respiratory pathogen Mannheimia haemolytica","volume":"13","author":"Uddin","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hazan, A., Lee, H.Y., Tiong, V., and AbuBakar, S. (2025). Bacillus subtilis Spores as a Vaccine Delivery Platform: A Tool for Resilient Health Defense in Low- and Middle-Income Countries. Vaccines, 13.","DOI":"10.3390\/vaccines13100995"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tvjl.2017.11.006","article-title":"Construction of a ptfA chitosan nanoparticle DNA vaccine against Pasteurella multocida and the immune response in chickens","volume":"231","author":"Gong","year":"2018","journal-title":"Vet. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s43141-021-00180-9","article-title":"The immune response and protective efficacy of a potential DNA vaccine against virulent Pasteurella multocida","volume":"19","author":"Yassein","year":"2021","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tvjl.2007.07.019","article-title":"Serotyping of Mannheimia haemolytica isolates from bovine pneumonia: 1987\u20132006","volume":"178","author":"Katsuda","year":"2008","journal-title":"Vet. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/s41541-024-01015-7","article-title":"A multi-epitope subunit vaccine providing broad cross-protection against diverse serotypes of Streptococcus suis","volume":"9","author":"Liu","year":"2024","journal-title":"npj Vaccines"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e0027222","DOI":"10.1128\/iai.00272-22","article-title":"Combinations but Not a Single PlpE Epitope Induce Host Protective Immunity against Pasteurella multocida","volume":"91","author":"Zhu","year":"2023","journal-title":"Infect. Immun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0168-1656(00)00309-6","article-title":"Pasteurella multocida capsule: Composition, function and genetics","volume":"83","author":"Boyce","year":"2000","journal-title":"J. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1128\/JCM.02824-14","article-title":"Development of a rapid multiplex PCR assay to genotype Pasteurella multocida strains using the lipopolysaccharide outer core biosynthesis locus","volume":"53","author":"Harper","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1093\/glycob\/cwu110","article-title":"Characterization of the lipopolysaccharide produced by Pasteurella multocida serovars 6, 7, and 16: Identification of lipopolysaccharide genotypes L4 and L8","volume":"25","author":"Harper","year":"2015","journal-title":"Glycobiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1007\/s00253-024-13060-2","article-title":"Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages","volume":"108","author":"Sun","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/S0264-410X(98)80118-7","article-title":"Haemorrhagic septicaemia vaccines","volume":"16","author":"Verma","year":"1998","journal-title":"Vaccine"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Fegan, J.E., Waeckerlin, R.C., Tesfaw, L., Islam, E.A., Deresse, G., Dufera, D., Assefa, E., Woldemedhin, W., Legesse, A., and Akalu, M. (2024). Developing a PmSLP3-based vaccine formulation that provides robust long-lasting protection against hemorrhagic septicemia\u2013causing serogroup B and E strains of Pasteurella multocida in cattle. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1392681"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Jin, C., Li, K., Yuan, H., Zhang, X., Rahim, M.F., Lu, Y., Mu, S., Wu, S., Su, H., and He, X. (2026). Outer Membrane Vesicles Derived from Yak Isolates of Pasteurella multocida Exhibit Promising Vaccine Potential. Animals, 16.","DOI":"10.3390\/ani16081264"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Tesfaye, A.B., Werid, G.M., Tao, Z., You, L., Han, R., Zhu, J., Fu, L., and Chu, Y. (2025). Advances in Pasteurella multocida Vaccine Development: From Conventional to Next-Generation Strategies. Vaccines, 13.","DOI":"10.3390\/vaccines13101034"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1007\/s10123-025-00658-3","article-title":"Virulence genes of Pasteurella multocida cap B and its potential cross protection in mice","volume":"28","author":"Tawor","year":"2025","journal-title":"Int. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Guan, L.J., Yang, J.Q., Xu, Q.Y., Feng, Y.-F., Zhang, X.-C., Tang, B., and Zhao, Z.-Q. (2023). Immunogenicity and efficacy of serogroup A and D bacterins against Pasteurella multocida in mice. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1132536"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Xu, Q., Guan, L., Xue, Y., and Zhao, Z. (2025). Natural Attenuated Vaccines for Pasteurella multocida. Biology, 14.","DOI":"10.3390\/biology14111466"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4140","DOI":"10.1016\/j.vaccine.2007.03.005","article-title":"Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE) vaccine","volume":"25","author":"Wu","year":"2007","journal-title":"Vaccine"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"41","DOI":"10.29252\/ibj.25.1.41","article-title":"In silico analysis of Pasteurella multocida PlpE protein epitopes as novel subunit vaccine candidates","volume":"25","author":"Mostaan","year":"2021","journal-title":"Iran. Biomed. J."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, Y., Xiao, J., Chang, Y., Zhang, H., Teng, Y., Lin, W., Li, H., Chen, W., Zhang, X., and Xie, Q. (2022). Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.985993"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.1016\/j.vaccine.2024.04.020","article-title":"Dendritic cell targeting peptide plus Salmonella FliCd flagellin fused outer membrane protein H (OmpH) demonstrated increased efficacy against infections caused by different Pasteurella multocida serogroups in mouse models","volume":"42","author":"Wang","year":"2024","journal-title":"Vaccine"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1590\/S1517-83822011000200043","article-title":"Immune response to a DNA vaccine expressing the transferrin-binding protein A gene of Pasteurella multocida","volume":"42","author":"Singh","year":"2011","journal-title":"Braz. J. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1006\/mpat.1999.0297","article-title":"Protective capacity of the Pasteurella haemolytica transferrin-binding proteins TbpA and TbpB in cattle","volume":"27","author":"Potter","year":"1999","journal-title":"Microb. Pathog."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"S263","DOI":"10.12980\/APJTB.4.2014C554","article-title":"Evaluation of immuno efficiency of hemorrhagic septicemia vaccine strain (vaccine seed)","volume":"4","author":"Gowrakkal","year":"2014","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1016\/j.rvsc.2012.05.011","article-title":"Immunogenicity and protective efficacy of recombinant Pasteurella multocida lipoprotein E and outer membrane protein H in mice","volume":"93","author":"Okaya","year":"2012","journal-title":"Res. Vet. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Tang, J., Al Amin, M., and Campian, J.L. (2025). Past, Present, and Future of Viral Vector Vaccine Platforms: A Comprehensive Review. Vaccines, 13.","DOI":"10.3390\/vaccines13050524"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Apinda, N., Yao, Y., Zhang, Y., Reddy, V.R.A.P., Chang, P., Nair, V., and Sthitmatee, N. (2022). CRISPR\/Cas9 Editing of Duck Enteritis Virus Genome for the Construction of a Recombinant Vaccine Vector Expressing ompH Gene of Pasteurella multocida in Two Novel Insertion Sites. Vaccines, 10.","DOI":"10.3390\/vaccines10050686"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Zhou, G., Tian, J., Tian, Y., Ma, Q., Li, Q., Wang, S., and Shi, H. (2023). A recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-023-03679-0"},{"key":"ref_59","first-page":"2168","article-title":"Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida","volume":"55","author":"Gao","year":"2024","journal-title":"Acta Vet. Et Zootech. Sin."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"126532","DOI":"10.1016\/j.vaccine.2024.126532","article-title":"Immunoprotective efficacy of Escherichia coli-derived outer membrane vesicles displaying the PlpE protein of Pasteurella multocida","volume":"44","author":"Li","year":"2025","journal-title":"Vaccine"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Tapiolas, M., Gibert, M., Montbrau, C., Taberner, E., Sol\u00e9, M., Tom\u00e1s, H.S., Puig, A., and March, R. (2024). Efficacy of a New Multivalent Vaccine for the Control of Bovine Respiratory Disease (BRD) in a Randomized Clinical Trial in Commercial Fattening Units. Vaccines, 12.","DOI":"10.1101\/2024.08.07.606972"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1016\/j.vaccine.2012.01.063","article-title":"Cross-protection of a Mannheimia haemolytica A1 Lkt\u2212\/Pasteurella multocida \u0394hyaE bovine respiratory disease vaccine against experimental challenge with Mannheimia haemolytica A6 in calves","volume":"30","author":"Crouch","year":"2012","journal-title":"Vaccine"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Gokduman, E.M., Atasoy, M.O., Goksu, A.G., Sozdutmaz, \u0130., and Munir, M. (2025). Potential of Bovine Herpesvirus Vectors for Recombinant Vaccines. Vaccines, 14.","DOI":"10.3390\/vaccines14010006"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Williams, L.B.A., Fry, L.M., Herndon, D.R., Franceschi, V., Schneider, D.A., Donofrio, G., and Knowles, D.P. (2019). A recombinant bovine herpesvirus-4 vectored vaccine delivered via intranasal nebulization elicits viral neutralizing antibody titers in cattle. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0215605"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"108492","DOI":"10.1016\/j.micpath.2026.108492","article-title":"Development and Evaluation of a P. multocida A-M. haemolytica A6-rLkt Combined Vaccine for Enhanced Control of Bovine Respiratory Disease Complex","volume":"216","author":"Yu","year":"2026","journal-title":"Microb. Pathog."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/avj.12995","article-title":"Health and production effects of killed vaccines against Mannheimia haemolytica, bovine viral diarrhea virus, and bovine herpesvirus 1 in locally backgrounded feedlot cattle","volume":"99","author":"Cusack","year":"2021","journal-title":"Aust. Vet. J."},{"key":"ref_67","first-page":"734","article-title":"The effect of subunit or modified live bovine herpesvirus-1 vaccines on the efficacy of a recombinant Pasteurella haemolytica vaccine for the prevention of respiratory disease in feedlot calves","volume":"33","author":"Harland","year":"1992","journal-title":"Can. Vet. J."},{"key":"ref_68","first-page":"239","article-title":"The effect of a combined Pasteurella haemolytica and Haemophilus somnus vaccine and a modified-live bovine respiratory syncytial virus vaccine against enzootic pneumonia in young beef calves","volume":"35","author":"Potter","year":"1994","journal-title":"Can. Vet. J."},{"key":"ref_69","first-page":"93","article-title":"Five field trials on the efficacy of a bovine respiratory syncytial virus vaccine","volume":"31","author":"Janzen","year":"1990","journal-title":"Can. Vet. J."},{"key":"ref_70","first-page":"163","article-title":"Efficacy of parenteral antibiotics for disease prophylaxis in feedlot calves","volume":"32","author":"Harland","year":"1991","journal-title":"Can. Vet. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e70608","DOI":"10.1002\/vms3.70608","article-title":"Epidemiological Investigation and Phylogenetic Analysis of Bovine Respiratory Disease Complex in Northern China","volume":"11","author":"Zhou","year":"2025","journal-title":"Vet. Med. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"van der Sluijs, M.T., Kuhn, E.M., and Makoschey, B. (2010). A single vaccination with an inactivated bovine respiratory syncytial virus vaccine primes the cellular immune response in calves with maternal antibodies. BMC Vet. Res., 6.","DOI":"10.1186\/1746-6148-6-2"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Meyer, G., Foret-Lucas, C., Delverdier, M., Cuquemelle, A., Secula, A., and Cassard, H. (2023). Protection against Bovine Respiratory Syncytial Virus Afforded by Maternal Antibodies from Cows Immunized with an Inactivated Vaccine. Vaccines, 11.","DOI":"10.3390\/vaccines11010141"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.vaccine.2003.08.033","article-title":"Maternally derived humoral immunity to bovine viral diarrhea virus (BVDV) 1a, BVDV1b, BVDV2, bovine herpesvirus-1, parainfluenza-3 virus, bovine respiratory syncytial virus, Mannheimia haemolytica, and Pasteurella multocida in beef calves: Antibody decline by half-life studies and effect on response to vaccination","volume":"22","author":"Fulton","year":"2004","journal-title":"Vaccine"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1002\/vetr.83","article-title":"NASYM, a live intranasal vaccine, protects young calves from bovine respiratory syncytial virus in the presence of maternal antibodies","volume":"188","author":"Marzo","year":"2021","journal-title":"Vet. Rec."},{"key":"ref_76","first-page":"247","article-title":"Epidemiological study of enzootic pneumonia in dairy calves in Saskatchewan","volume":"57","author":"Ribble","year":"1993","journal-title":"Can. J. Vet. Res."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ijmm.2013.05.001","article-title":"Immunogenicity of Pasteurella multocida and Mannheimia haemolytica outer membrane vesicles","volume":"303","author":"Roier","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1128\/JB.183.4.1394-1404.2001","article-title":"Sequence diversity and molecular evolution of the leukotoxin (lktA) gene in bovine and ovine strains of Mannheimia (Pasteurella) haemolytica","volume":"183","author":"Davies","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"589","DOI":"10.3168\/jds.2022-21929","article-title":"Pathogenic infection characteristics and risk factors for bovine respiratory disease complex based on the detection of lung pathogens in dead cattle in Northeast China","volume":"106","author":"Zhou","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11259-026-11256-7","article-title":"Epidemiological surveillance of BVDV, Pasteurella multocida, and Brucella spp. infections in cattle from Guizhou, China using a newly established triplex TaqMan qPCR assay","volume":"50","author":"Hu","year":"2026","journal-title":"Vet. Res. Commun."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Wang, C., Dou, L., Wang, J., Ye, D., and Yang, Z. (2025). Molecular epidemiology and antibiotic resistance of sheep-derived Mannheimia haemolytica in northwestern China. Animals, 15.","DOI":"10.3390\/ani15233492"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1093\/jac\/dkad365","article-title":"Genomic analysis reveals the population structure and antimicrobial resistance of avian Pasteurella multocida in China","volume":"79","author":"Jiang","year":"2024","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_83","first-page":"287","article-title":"Prevalence and relative-risks of pasteurella in yaks of Xizang, China","volume":"57","author":"Ma","year":"2025","journal-title":"Prev. Vet. Med."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.vetimm.2017.11.004","article-title":"In silico identification and high-throughput screening of antigenic proteins as candidates for a Mannheimia haemolytica vaccine","volume":"195","author":"Klima","year":"2018","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"e1901071","DOI":"10.1002\/mnfr.201901071","article-title":"\u03b2-1,3\/1,6-Glucans and Immunity: State of the Art and Future Directions","volume":"65","author":"Calder","year":"2021","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1016\/j.vaccine.2008.09.028","article-title":"Immunity of cattle following vaccination with Mannheimia haemolytica chimeric PlpE-LKT (SAC89) protein","volume":"27","author":"Confer","year":"2009","journal-title":"Vaccine"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2911\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T08:32:44Z","timestamp":1789547564000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":86,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16182911"],"URL":"https:\/\/doi.org\/10.3390\/ani16182911","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}