{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:11:29Z","timestamp":1789992689360,"version":"4.0.1"},"reference-count":50,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002848","name":"National Research and Development Agency of Chile","doi-asserted-by":"publisher","award":["1191114"],"award-info":[{"award-number":["1191114"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"National Research and Development Agency of Chile","doi-asserted-by":"publisher","award":["1231145"],"award-info":[{"award-number":["1231145"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Organoids serve as three-dimensional structures that offer spatial support and mimic cellular niches for experimental studies. The spatial and temporal association of fetal bovine adipose tissue-derived mesenchymal stem\/stromal cells (AT-MSCs) with the expression of germ cell (GC) markers was evaluated in testicular organoids (TOs+AT-MSCs) under the effect of retinoic acid and testosterone (0.2, 2 and 20 \u00b5M). TOs+AT-MSCs were developed by the co-culture of Leydig, Sertoli, and peritubular myoid cells and PKH26+AT-MSCs and were analyzed for the expression of DAZL, STRA8 and UCHL1. TOs+AT-MSCs treated with 1 \u00b5M of retinoic acid and 2 or 20 \u00b5M of testosterone maintained their diameter after day 7 of culture. Pearson correlation coefficients (PCCs) of PKH26 and DAZL were higher (p &lt; 0.05) at days 7 and 21 in TOs+AT-MSCs treated with 1 \u00b5M retinoic acid and 0.2 or 2 \u00b5M of testosterone, respectively. PCCs of PKH26 and STRA8 or UCHL1 were higher (p &lt; 0.05) at days 14 and 21 in TOs+AT-MSCs treated with 1 \u00b5M retinoic acid and 0.2 or 2 \u00b5M of testosterone. Thus, exposure to 1 \u00b5M of retinoic acid and 2 \u00b5M of testosterone resulted in a similar diameter with respect to the control and spatial and temporal association of AT-MSCs and DAZL, STRA8 and UCHL1 markers in TOs+AT-MSCs.<\/jats:p>","DOI":"10.3390\/ani16182933","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2933","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Associations of Bovine Fetal Adipose Tissue-Derived Mesenchymal Stem\/Stromal Cells and Germ Cell Marker Expression in Testicular Organoids"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1819-8524","authenticated-orcid":false,"given":"Jahaira","family":"Cortez","sequence":"first","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, Santiago 8820808, Chile"},{"name":"Doctorate Program of Forestry, Agriculture, and Veterinary Sciences (DCSAV), University of Chile, Santa Rosa 11315, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3621-2705","authenticated-orcid":false,"given":"V\u00edctor H.","family":"Parraguez","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary and Animal Sciences, University of Chile, Santa Rosa 11735, Santiago 8820808, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7907-4978","authenticated-orcid":false,"given":"Carlos","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Programa de Doctorado en Ciencias, Escuela Internacional de Doctorado de la UNED (EIUNED), Bravo Murillo 38, 28015 Madrid, Spain"},{"name":"Escuela de Medicina Veterinaria, Facultad de Agronom\u00eda y Sistemas Naturales, Facultad de Ciencias Biol\u00f3gicas y Facultad de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Vicu\u00f1a Mackenna 4860, Santiago 7820436, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2040-4902","authenticated-orcid":false,"given":"Oscar A.","family":"Peralta","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Agronom\u00eda y Sistemas Naturales, Facultad de Ciencias Biol\u00f3gicas y Facultad de Medicina, Pontificia Universidad Cat\u00f3lica de Chile, Vicu\u00f1a Mackenna 4860, Santiago 7820436, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Koller, M.R., Palsson, B.O., and Masters, J.R. 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