{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:07:52Z","timestamp":1790053672467,"version":"4.0.1"},"reference-count":45,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","award":["600-2095\/20"],"award-info":[{"award-number":["600-2095\/20"]}],"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"crossref"}]},{"award":["600-2095\/20"],"award-info":[{"award-number":["600-2095\/20"]}],"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}]},{"name":"UNLP","award":["V273"],"award-info":[{"award-number":["V273"]}],"id":[{"id":"https:\/\/ror.org\/01tjs6929","id-type":"ROR","asserted-by":"crossref"}]},{"name":"UNLP","award":["V291"],"award-info":[{"award-number":["V291"]}],"id":[{"id":"https:\/\/ror.org\/01tjs6929","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Mugil liza, an economic resource fish species along the South American Atlantic coast, serves as the sole mullet species breeding in Argentina. While encysted parasites in mullets typically target the bulbus arteriosus, this study identifies Ascocotyle sp. metacercariae localized exclusively within the cardiac ventricle of Mugil liza. Thirty juvenile specimens were collected from Samboromb\u00f3n Bay, Argentina, to evaluate the resulting cardiac lesions and associated inflammatory host response through histological, lectinhistochemical, and immunohistochemical analyses. Pathological evaluation revealed marked disruption of the myocardial architecture, characterized by regions of coagulative necrosis, presence of melanomacrophage centers (MMCs), and congested blood vessels containing cellular debris within their lumen. A persistent mononuclear inflammatory infiltrate\u2014comprising eosinophilic granular cells, macrophages, and lymphocytes\u2014was observed surrounding the ventricular cysts and extending into the atrium. Lectinhistochemical characterization identified a shared glycan profile, marked by positivity for Phaseolus vulgaris leucoagglutinin (PHA-L) and Pisum sativum agglutinin (PSA), across monocyte and macrophage lineages. This specific carbohydrate expression highlights the active participation and recruitment of these immune cell populations during the host defense. Overall, these pathological and glycobiological findings demonstrate that Ascocotyle sp. metacercariae act as a chronic immunogenic stimulus, driving and sustaining localized myocardial inflammation in M. liza.<\/jats:p>","DOI":"10.3390\/ani16182939","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Histological and Lectinhistochemical Pattern of the Inflammatory Response to Ascocotyle sp. Metacercariae in Mugil liza Hearts"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9142-6938","authenticated-orcid":false,"given":"Luca","family":"Di Cesare","sequence":"first","affiliation":[{"name":"Instituto de Morfolog\u00eda y Patolog\u00eda Animal (IMPA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"},{"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE, CCT, CONICET), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"},{"name":"C\u00e1tedra de Bioqu\u00edmica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Claudio G.","family":"Barbeito","sequence":"additional","affiliation":[{"name":"Instituto de Morfolog\u00eda y Patolog\u00eda Animal (IMPA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Jorge","family":"Barneche","sequence":"additional","affiliation":[{"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE, CCT, CONICET), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-333X","authenticated-orcid":false,"given":"Mart\u00edn M.","family":"Montes","sequence":"additional","affiliation":[{"name":"Centro de Estudios Parasitol\u00f3gicos y Vectores (CEPAVE, CCT, CONICET), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7318-7863","authenticated-orcid":false,"given":"Silvia E.","family":"Plaul","sequence":"additional","affiliation":[{"name":"Instituto de Morfolog\u00eda y Patolog\u00eda Animal (IMPA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.ympev.2012.03.006","article-title":"Systematics of the grey mullets (Teleostei: Mugiliformes: Mugilidae): Molecular phylogenetic evidence challenges two centuries of morphology-based taxonomy","volume":"64","author":"Durand","year":"2012","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ecss.2014.07.013","article-title":"Microsatellite variation and genetic structuring in Mugil liza (Teleostei: Mugilidae) populations from Argentina and Brazil","volume":"149","author":"Mai","year":"2014","journal-title":"Estuar. Coast. Shelf Sci."},{"key":"ref_3","first-page":"67","article-title":"Revis\u00e3o e discuss\u00e3o de trabalhos sobre as esp\u00e9cies do g\u00eanero Mugil (Teleostei, Perciformes, Mugilidae) da costa brasileira","volume":"15","author":"Godinho","year":"1988","journal-title":"Bol. Inst. Pesca"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1080\/11250003.2010.549154","article-title":"Studies on reproduction of the mullet Mugil platanus G\u00fcnther, 1880 (Actinopterygii, Mugilidae) from the Mar Chiquita coastal lagoon, Argentina: Similarities and differences with related species","volume":"78","author":"Macchi","year":"2011","journal-title":"Ital. J. Zool."},{"key":"ref_5","first-page":"30","article-title":"Heterophiasis: An illness from fish to human","volume":"28","author":"Perretta","year":"2006","journal-title":"INFOPESCA Int."},{"key":"ref_6","first-page":"19","article-title":"Identificaci\u00f3n en Uruguay de metacercarias de Ascocotyle (Phagicola) longa Digenea: Heterophyidae parasitando lisas, Mugil platanus Pisces: Mugilidae y evaluaci\u00f3n del riesgo de zoonosis y afecciones en mascotas","volume":"40","author":"Carnevia","year":"2005","journal-title":"Veterinaria"},{"key":"ref_7","first-page":"3","article-title":"The heterophyid trematode Ascocotyle (a.) leighi Burton, 1956, from the hearts of certain poeciliid and cyprinodont fishes","volume":"24","author":"Lumsden","year":"1964","journal-title":"Z. Parasitenkd."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1080\/00222930152667087","article-title":"Trematodes of the family Heterophyidae (Digenea) in Mexico: A review of species and new host and geographical records","volume":"35","author":"Scholz","year":"2001","journal-title":"J. Nat. Hist."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.ijppaw.2018.12.008","article-title":"Heterophyid trematodes (Digenea) from penguins: A new species of Ascocotyle Loss, 1899, first description of metacercaria of Ascocotyle (A.) patagoniensis Hern\u00e1ndez-Orts, Montero, Crespo, Garc\u00eda, Raga and Aznar, 2012, and first molecular data","volume":"8","author":"Georgieva","year":"2019","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.vetpar.2012.07.002","article-title":"Morphological and molecular identification of the fish-borne metacercaria of Ascocotyle (Phagicola) longa Ransom, 1920 in Mugil liza from Argentina","volume":"190","author":"Martorelli","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_11","first-page":"119","article-title":"Life cycles of two new sibling species of Ascocotyle (Ascocotyle) (Digenea, Heterophyidae) in the Neotropical Region","volume":"46","year":"2001","journal-title":"Acta Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.parint.2016.05.006","article-title":"High-intensity cardiac infections of Phthinomita heinigerae n. sp. (Digenea: Aporocotylidae) in the orange lined cardinalfish, Taeniamia fucata (Cantor), of Heron Island on the great Barrier Reef","volume":"65","author":"Nolan","year":"2016","journal-title":"Parasitol. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.aquaculture.2016.11.009","article-title":"Endocarditis associated with blood fluke infections (Digenea: Aporocotylidae: Psettarium cf. anthicum) among aquacultured cobia (Rachycentron canadum) from Nha Trang Bay, Vietnam","volume":"468","author":"Warren","year":"2017","journal-title":"Aquaculture"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3354\/dao066245","article-title":"Histopathology, ultrastructure and immunohistochemistry of Coregonus lavaretus hearts naturally infected with Ichthyocotylurus erraticus (Trematoda)","volume":"66","author":"Dezfuli","year":"2005","journal-title":"Dis. Aquat. Org."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.fsi.2005.01.012","article-title":"Inflammatory cells of teleostean fish: A review focusing on mast cells\/eosinophilic granule cells and rodlet cells","volume":"20","author":"Reite","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"ref_16","first-page":"59","article-title":"Eosinophilic Granular Cells\/Mast Cells (EGC\/MC) and their relationship with the effects of herbicides: The case of glyphosate and accompanying surfactants in fish","volume":"19","year":"2015","journal-title":"Orinoquia"},{"key":"ref_17","first-page":"118","article-title":"Inflammatory reaction induced by Floridosentis mugili (Acanthocephala, Neoechinorhynchidae) in the intestine of Mugil liza","volume":"41","author":"Plaul","year":"2021","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mokhtar, D.M., Zaccone, G., Alesci, A., Kuciel, M., Hussein, M.T., and Sayed, R.K.A. (2023). Main Components of Fish Immunity: An Overview of the Fish Immune System. Fishes, 8.","DOI":"10.3390\/fishes8020093"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Steinel, N.C., and Bolnick, D.I. (2017). Melanomacrophage Centers as a Histological indicator of immune function in fish and other Poikilotherms. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.00827"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1111\/j.1365-2761.1990.tb00814.x","article-title":"Lectin histochemistry as special markers for rodlet cells in carp, Cyprinus carpio L.","volume":"13","author":"Imagawa","year":"1990","journal-title":"J. Fish Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/jfd.12751","article-title":"Histochemical and immunohistochemical characterization of rodlet cells in the intestine of two teleosts, Anguilla anguilla and Cyprinus carpio","volume":"41","author":"Bosi","year":"2018","journal-title":"J. Fish Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.15517\/rbt.v64i1.18235","article-title":"Histochemical differences along the intestine of Corydoras paleatus (Siluriformes, Callichthyidae)","volume":"64","author":"Plaul","year":"2016","journal-title":"Rev. Biol. Trop."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1021\/acschembio.1c00689","article-title":"A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities","volume":"17","author":"Bojar","year":"2022","journal-title":"ACS Chem. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3354\/dao010173","article-title":"Lectin histochemistry and ultrastructure of rainbow trout Oncorhynchus mykiss kidneys affected by proliferative kidney","volume":"10","author":"Castagnaro","year":"1991","journal-title":"Dis. Aquat. Org."},{"key":"ref_25","first-page":"17","article-title":"Sobre la utilizaci\u00f3n de trampas para peces en las lagunas pamp\u00e1sicas","volume":"6","author":"Colautti","year":"1998","journal-title":"Rev. Ictiol."},{"key":"ref_26","first-page":"21","article-title":"Utilizaci\u00f3n del aceite de clavo, Syzygium aromaticum L. (Merr. & Perry), como anest\u00e9sico eficaz y econ\u00f3mico para labores rutinarias de manipulaci\u00f3n de peces marinos cultivados","volume":"18","author":"Raja","year":"2002","journal-title":"Bol. Inst. Esp. Oceanogr."},{"key":"ref_27","unstructured":"M\u00e9ndez-Vilas, A. (2017). Morphological techniques used in ichthyopathological diagnosis. Microscopy and Imaging Science Approaches to Applied Research and Education, Formatex Research Center. Available online: http:\/\/hdl.handle.net\/11336\/163386."},{"key":"ref_28","first-page":"630","article-title":"Ovarian changes and development of the branchial placenta occurring in Jenynsia lineata (Cyprinodontiformes, Anablepidae)","volume":"284","author":"Barbeito","year":"2023","journal-title":"J. Morphol."},{"key":"ref_29","first-page":"202","article-title":"Parasitological and histopathological study of digenetic trematodes in mullets from Lake Qarun, Egypt","volume":"13","author":"Kotb","year":"2014","journal-title":"Glob. Vet."},{"key":"ref_30","first-page":"4","article-title":"Histological effect of Ascocotyle tenuicollis (Digenea:Heterophyidae) metacercarial infection on the heart of Fundulus heteroclitus (Teleostei:Cyprinodontidae)","volume":"1","author":"Hicks","year":"2003","journal-title":"J. South Carol. Acad. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1002\/ar.20507","article-title":"Rodlet cells and the sensory systems in zebrafish (Danio rerio)","volume":"290","author":"Dezfuli","year":"2007","journal-title":"Anat. Rec."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3390\/biology4040881","article-title":"Biology of Bony Fish Macrophages","volume":"4","author":"Hodgkinson","year":"2015","journal-title":"Biology"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"De Benedetto, G., Giannetto, A., Riolo, K., Iaria, C., Brianti, E., and Gaglio, G. (2021). Anisakis pegreffii larvae in Sphyraena viridensis and description of granulomatous lesions. Animals, 11.","DOI":"10.3390\/ani11123449"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Polinas, M., Padr\u00f3s, F., Merella, P., Prearo, M., Sanna, M.A., Marino, F., Burrai, G.P., and Antuofermo, E. (2021). Stages of Granulomatous Response Against Histozoic Metazoan Parasites in Mullets (Osteichthyes: Mugilidae). Animals, 11.","DOI":"10.3390\/ani11061501"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3354\/dao02971","article-title":"Histopathological alterations of the heart in fish: Proposal for a standardized assessment","volume":"118","author":"Steinbach","year":"2016","journal-title":"Dis. Aquat. Org."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.molimm.2015.09.026","article-title":"Polarization of immune responses in fish: The \u2018macrophages first\u2019 point of view","volume":"69","author":"Wiegertjes","year":"2016","journal-title":"Mol. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e70282","DOI":"10.1002\/iid3.70282","article-title":"Role of macrophages in cardiac remodeling: Cues from zebrafish heart","volume":"14","author":"Gaur","year":"2026","journal-title":"Immun. Inflamm. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Luz, R.B.D.S., Paula, A.G.P., Czaikovski, A.P., Nunes, B.S.F., De Lima, J.D., Paredes, L.C., Bastos, T.S.B., Richardson, R., and Braga, T.T. (2025). Macrophages and cardiac lesion in zebrafish: What can single-cell RNA sequencing reveal?. Front. Cardiovasc. Med., 12.","DOI":"10.3389\/fcvm.2025.1570582"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1046\/j.1365-2761.2003.00485.x","article-title":"Melano-macrophage centers and their role in fish pathology","volume":"26","author":"Agius","year":"2003","journal-title":"J. Fish Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"689","DOI":"10.21608\/ejabf.2021.193982","article-title":"The antibacterial activity of melanin in the Cuttlefish (Sepia sp.) ink against Aeromonas sp.","volume":"25","author":"Fitrial","year":"2021","journal-title":"Egyp. J. Aquat. Biol. Fish."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Grayfer, L., Kerimoglu, B., Yaparla, A., Hodgkinson, J.W., Xie, J., and Belosevic, M. (2018). Mechanisms of fish macrophage antimicrobial immunity. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01105"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.devbrainres.2004.10.008","article-title":"Patterns of cell proliferation and cell death in the developing retina and optic tectum of the brown trout","volume":"154","author":"Candal","year":"2005","journal-title":"Brain Res. Dev. Brain Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.fsi.2016.02.006","article-title":"Immunolocalization of immune cells and cell cycle proteins in the bulbus arteriosus of Atlantic salmon (Salmo salar L.)","volume":"51","author":"Yousaf","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1093\/cvr\/cvz221","article-title":"Specific macrophage populations promote both cardiac scar deposition and subsequent resolution in adult zebrafish","volume":"116","author":"Bevan","year":"2020","journal-title":"Cardiovasc. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1177\/030098588902600508","article-title":"Chronic inflammatory cells with epithelial cell characteristics in teleost fishes","volume":"26","author":"Noga","year":"1989","journal-title":"Vet. Pathol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2939\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:40:42Z","timestamp":1790052042000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/18\/2939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":45,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ani16182939"],"URL":"https:\/\/doi.org\/10.3390\/ani16182939","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}