{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:54:28Z","timestamp":1790916868210,"version":"4.1.0"},"reference-count":84,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-04741-24"],"award-info":[{"award-number":["APQ-04741-24"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["BIP-00140-23"],"award-info":[{"award-number":["BIP-00140-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"award":["APQ-04741-24"],"award-info":[{"award-number":["APQ-04741-24"]}],"id":[{"id":"https:\/\/ror.org\/00nc55f03","id-type":"ROR","asserted-by":"publisher"}]},{"award":["BIP-00140-23"],"award-info":[{"award-number":["BIP-00140-23"]}],"id":[{"id":"https:\/\/ror.org\/00nc55f03","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404383\/2023-8"],"award-info":[{"award-number":["404383\/2023-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["404383\/2023-8"],"award-info":[{"award-number":["404383\/2023-8"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The presence of hydroxyapatite (HAp) in the Cuticle of laying-hen eggshells was investigated through a comprehensive characterization of its morphology, composition, crystallographic structure, and thermal behavior. Comparison with bone-derived hydroxyapatite confirmed the similarity of the mineral phase. Examination of the same region at different imaging contrasts revealed unidirectional (nanofibrous) calcite growth within the Vertical Layer (VL) and Palisade Layer (PL). Shell thickening in these layers appears to occur through an additive mechanism involving the successive deposition of nanodroplets containing, according to our hypothesis, the mineral phase, water, and organic components. This multiphasic system generates lamellae that progressively increase in thickness through the continuous incorporation of new nanodroplets onto the pre-existing surface. Such a nanodroplet-mediated growth mechanism also provides a plausible explanation for the formation of the micropores observed in the VL and PL. The proposed additive mechanism is further supported by the presence of nanohemispheres attached to growing lamellae and the similar diameters of cuticle nanospheres and Vertical Layer nanohemispheres. Fractures observed in the Vertical Layer indicate structural continuity between the Cuticle and the VL, suggesting that additive growth involves a continuous supply of HAp, possibly across the entire uterine surface, followed by a previously undescribed process in which calcium phosphate nanospheres dissolve and\/or transform into calcium carbonate nanofibers.<\/jats:p>","DOI":"10.3390\/ani16193023","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:36:34Z","timestamp":1790346994000},"page":"3023","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phosphate and Carbonate in the Biomineralization of Chicken Eggshells and the Increase in Eggshell Thickness Through Nanodroplet Addition"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-5157","authenticated-orcid":false,"given":"Antonio Valad\u00e3o","family":"Cardoso","sequence":"first","affiliation":[{"name":"School of Design, State University of Minas Gerais (UEMG), Belo Horizonte 30140-091, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5987-8622","authenticated-orcid":false,"given":"Rodrigo Novaes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Microscopy Center, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Sylvia","family":"Dantas","sequence":"additional","affiliation":[{"name":"Metallurgy Department, School of Engineering, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1418-5048","authenticated-orcid":false,"given":"Leonardo H. 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Dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Characterization and Processing of Nanomaterials, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara Luz de","family":"Assis","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1080\/1828051X.2017.1344935","article-title":"Relationship between eggshell thickness and other eggshell measurements in eggs from litter and cages","volume":"17","author":"Ketta","year":"2018","journal-title":"Ital. J. Anim. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"338","DOI":"10.2141\/jpsa.0130032","article-title":"Effect of uniformity of eggshell thickness on eggshell quality in chickens","volume":"51","author":"Yan","year":"2014","journal-title":"J. Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.3382\/ps.2012-02220","article-title":"Global variation and uniformity of eggshell thickness for chicken eggs","volume":"91","author":"Sun","year":"2012","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"101287","DOI":"10.1016\/j.psj.2021.101287","article-title":"Research Note: Changes in eggshell quality and microstructure related to hen age during a production cycle","volume":"100","author":"Folegatti","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Alig, B.N., Anderson, K.E., Malheiros, D.M., Harding, K.L., and Malheiros, R.D. (2025). Assessment of the effects of stocking density on laying hens raised in colony cages: Part II\u2014Egg production, egg quality, and welfare parameters. Poultry, 4.","DOI":"10.3390\/poultry4030028"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"103475","DOI":"10.1016\/j.psj.2024.103475","article-title":"A systematic review of potential productivity, egg quality, and animal welfare implications of extended lay cycles in commercial laying hens in Canada","volume":"103","author":"Arulnathan","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Noble, R., and Noble, D. (2023). Understanding Living Systems, Cambridge University Press.","DOI":"10.1017\/9781009277396"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"108425","DOI":"10.1016\/j.isci.2023.108425","article-title":"Attaching organic fibers to mineral: The case of the avian eggshell","volume":"26","author":"Buss","year":"2023","journal-title":"iScience"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"ziae176","DOI":"10.1093\/jbmrpl\/ziae176","article-title":"Understanding the structural biology of osteomalacia through multiscale 3D X-ray and electron tomographic imaging: A review of X-linked hypophosphatemia, the Hyp mouse model, and imaging methods","volume":"9","author":"Buss","year":"2025","journal-title":"JBMR Plus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0945-053X(97)90112-8","article-title":"Eggshells are shaped by a precise spatio-temporal arrangement of sequentially deposited macromolecules","volume":"16","author":"Fernandez","year":"1997","journal-title":"Matrix Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s00435-024-00676-1","article-title":"Eggshell ultrastructure and mineral composition during the osteogenesis of Kinosternon scorpioides (Testudines: Kinosternidae)","volume":"143","author":"Leal","year":"2024","journal-title":"Zoomorphology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0022-0981(76)90091-5","article-title":"Studies on the eggshell (oviducal and oviposited) of Chelonia mydas L.","volume":"22","author":"Solomon","year":"1976","journal-title":"J. Exp. Mar. Biol. Ecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.actbio.2015.11.040","article-title":"Hierarchical structure and mechanical properties of snake (Naja atra) and turtle (Ocadia sinensis) eggshells","volume":"31","author":"Chang","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"eaar3219","DOI":"10.1126\/sciadv.aar3219","article-title":"Nanostructure, osteopontin, and mechanical properties of calcitic avian eggshell","volume":"4","author":"Athanasiadou","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jsb.2015.04.014","article-title":"Amorphous calcium carbonate controls avian eggshell mineralization: A new paradigm for understanding rapid eggshell calcification","volume":"190","author":"Marie","year":"2015","journal-title":"J. Struct. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gautron, J., Stapane, L., Le Roy, N., Nys, Y., Rodriguez-Navarro, A.B., and Hincke, M.T. (2021). Avian eggshell biomineralization: An update on its structure, mineralogy and protein tool kit. BMC Mol. Cell Biol., 22, Correction in BMC Mol. Cell Biol.\u00a02021, 22, 1. https:\/\/doi.org\/10.1186\/s12860-021-00351-z.","DOI":"10.1186\/s12860-021-00351-z"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/(SICI)1097-4687(199606)228:3<287::AID-JMOR2>3.0.CO;2-#","article-title":"Microstructure of matrix and mineral components of eggshells from white leghorn chickens (Gallus gallus)","volume":"228","author":"Dennis","year":"1996","journal-title":"J. Morphol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"908","DOI":"10.3382\/ps.0330908","article-title":"The transfer of phosphorus to the hen\u2019s egg, under controlled environment, as traced with radiophosphorus (P32)","volume":"33","author":"Smith","year":"1954","journal-title":"Poult. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Garcia-Mejia, R.A., Sinclair-Black, M., Blair, L.R., Angel, R., Jaramillo, B., Regmi, P., Neupane, N., Proszkowiec-Weglarz, M., Arbe, X., and Cavero, D. (2024). Physiological changes in the regulation of calcium and phosphorus utilization that occur after the onset of egg production in commercial laying hens. Front. Physiol., 15.","DOI":"10.3389\/fphys.2024.1465817"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Coudert, E., Corrales, N., Coto, C., Menoyo, D., and Fondevila, G. (2025). The role of phosphorus sources and phytase in growth performance and feed digestibility in broilers. Animals, 15.","DOI":"10.3390\/ani15142111"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4170","DOI":"10.1038\/s41467-018-06570-x","article-title":"Transformation of amorphous calcium phosphate to bone-like apatite","volume":"9","author":"Lotsari","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1086\/693434","article-title":"What does the eggshell cuticle do? A functional comparison of avian eggshell cuticles","volume":"90","author":"Torres","year":"2017","journal-title":"Physiol. Biochem. Zool."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.3382\/ps.2013-03230","article-title":"Change in the chicken eggshell cuticle with hen age and egg freshness","volume":"92","year":"2013","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"103428","DOI":"10.1016\/j.psj.2024.103428","article-title":"Scanning electron microscope-based evaluation of eggshell quality","volume":"103","author":"Mahato","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"126978","DOI":"10.1016\/j.jcrysgro.2022.126978","article-title":"The transformation of amorphous calcium carbonate to calcite and classical nucleation theory","volume":"603","author":"Freeman","year":"2023","journal-title":"J. Cryst. Growth"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"299","DOI":"10.17221\/46\/2015-CJAS","article-title":"Eggshell structure, measurements, and quality-affecting factors in laying hens: A review","volume":"61","author":"Ketta","year":"2016","journal-title":"Czech. J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.5851\/kosfa.2018.e41","article-title":"The influence of hen aging on eggshell ultrastructure and shell mineral components","volume":"38","author":"Park","year":"2018","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/0305-0491(74)90011-X","article-title":"Chemical composition of the cuticle, and the inner and outer shell membranes from eggs of Gallus gallus","volume":"47","author":"Wedral","year":"1974","journal-title":"Comp. Biochem. Physiol. B"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.3382\/ps.0621860","article-title":"The relationship of dietary levels of phosphorus to the production of soft-shelled and shell-less eggs","volume":"62","author":"Klingensmith","year":"1983","journal-title":"Poult. Sci."},{"key":"ref_30","first-page":"185","article-title":"Premature oviposition induced by intrauterine injection of phosphate solution in the laying hen","volume":"46","author":"Ogasawara","year":"1975","journal-title":"Jpn. J. Zootech. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1902536","DOI":"10.1002\/advs.201902536","article-title":"Biomineral precursor formation is initiated by transporting calcium and phosphorus clusters from the endoplasmic reticulum to mitochondria","volume":"7","author":"Tang","year":"2020","journal-title":"Adv. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2300126","DOI":"10.1002\/advs.202301231","article-title":"Logistics of bone mineralization in the chick embryo studied by 3D cryo FIB-SEM imaging","volume":"10","author":"Raguin","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Sinclair-Black, M., Garcia, R.A., and Ellestad, L.E. (2023). Physiological regulation of calcium and phosphorus utilization in laying hens. Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1112499"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1469-185X.1964.tb01166.x","article-title":"Phosphates as crystal poisons of calcification","volume":"39","author":"Simkiss","year":"1964","journal-title":"Biol. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1021\/acs.cgd.0c01245","article-title":"Nonclassical crystallization of amorphous calcium carbonate in the presence of phosphate ions","volume":"21","author":"Zou","year":"2021","journal-title":"Cryst. Growth Des."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.gca.2023.01.028","article-title":"Transformation of amorphous calcium carbonate in the presence of magnesium, phosphate, and mineral surfaces","volume":"345","year":"2023","journal-title":"Geochim. Cosmochim. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s12274-010-0067-8","article-title":"Elaborate architecture of the hierarchical hen\u2019s eggshell","volume":"4","author":"Zhou","year":"2011","journal-title":"Nano Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.palaeo.2009.05.011","article-title":"Porosity of Late Paleocene Ornitholithus eggshells (Tremp Fm, south-central Pyrenees, Spain): Palaeoclimatic implications","volume":"279","author":"Donaire","year":"2009","journal-title":"Palaeogeogr. Palaeoclimatol. Palaeoecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1590\/S1516-635X2000000200007","article-title":"Pore size distribution in chicken eggs as determined by mercury porosimetry","volume":"2","author":"Boleli","year":"2000","journal-title":"Rev. Bras. Cienc. Avic."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5550","DOI":"10.1002\/jsfa.14290","article-title":"Non-destructive measurement of eggshell strength using NIR spectroscopy and explainable artificial intelligence","volume":"105","author":"Ahmed","year":"2025","journal-title":"J. Sci. Food Agric."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1080\/00071668.2014.924093","article-title":"Quantification of eggshell microstructure using X-ray micro computed tomography","volume":"55","author":"Riley","year":"2014","journal-title":"Br. Poult. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.micron.2018.12.008","article-title":"Study of the porosity of calcified chicken eggshell using atomic force microscopy and image processing","volume":"118","year":"2019","journal-title":"Micron"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.3382\/ps\/pex409","article-title":"Cuticle and pore plug properties in the table egg","volume":"97","author":"Kulshreshtha","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"325","DOI":"10.3109\/03008209609029207","article-title":"The avian eggshell extracellular matrix as a model for biomineralization","volume":"35","author":"Carrino","year":"1996","journal-title":"Connect. Tissue Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.2741\/3985","article-title":"The eggshell: Structure, composition and mineralization","volume":"17","author":"Hincke","year":"2012","journal-title":"Front. Biosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3444","DOI":"10.1016\/j.scib.2024.08.023","article-title":"Recent advancements and perspectives on processable natural biopolymers: Cellulose, chitosan, eggshell membrane, and silk fibroin","volume":"69","author":"Liang","year":"2024","journal-title":"Sci. Bull."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"15853","DOI":"10.1074\/jbc.RA120.014542","article-title":"Avian eggshell formation reveals a new paradigm for vertebrate mineralization via vesicular amorphous calcium carbonate","volume":"295","author":"Stapane","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.4315\/0362-028X.JFP-11-013","article-title":"Effect of egg washing on the cuticle quality of brown and white table eggs","volume":"74","author":"Leleu","year":"2011","journal-title":"J. Food Prot."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/1806-9061-2019-1046","article-title":"Methods to evaluate the eggshell quality of table eggs","volume":"21","author":"Montenegro","year":"2019","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1745-4603.1974.tb01099.x","article-title":"Measurement of eggshell strength","volume":"5","author":"Voisey","year":"1974","journal-title":"J. Texture Stud."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2475\/ajs.254.2.65","article-title":"The unit cells of calcite","volume":"254","author":"Winchell","year":"1956","journal-title":"Am. J. Sci."},{"key":"ref_52","unstructured":"Abbaschian, R., Abbaschian, L., and Reed-Hill, R.E. (2010). Physical Metallurgy Principles, Cengage Learning. [4th ed.]."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40104-023-00986-2","article-title":"Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens","volume":"15","author":"Fu","year":"2024","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/S0945-053X(00)00128-1","article-title":"Secretion pattern, ultrastructural localization and function of extracellular matrix molecules involved in eggshell formation","volume":"19","author":"Fernandez","year":"2001","journal-title":"Matrix Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/978-3-030-12919-4_5","article-title":"Chondroitin, dermatan, heparan, and keratan sulfate: Structure and functions","volume":"Volume 12","author":"Cohen","year":"2019","journal-title":"Extracellular Sugar-Based Biopolymers Matrices. Biologically-Inspired Systems"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1113\/expphysiol.1973.sp002199","article-title":"The ultrastructure of the shell forming region of the oviduct and the development of the shell of Gallus domesticus","volume":"58","author":"Wyburn","year":"1973","journal-title":"Q. J. Exp. Physiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0022-5320(63)80029-5","article-title":"The crystalline structure of calcium carbonate in the avian eggshell: An electron microscope study","volume":"8","author":"Heyn","year":"1963","journal-title":"J. Ultrastruct. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1016\/j.biomaterials.2007.02.028","article-title":"Micro-Raman and FTIR studies of synthetic and natural apatites","volume":"28","author":"Antonakos","year":"2007","journal-title":"Biomaterials"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jssc.2017.07.025","article-title":"Carbonate substitution in the mineral component of bone: Discriminating the structural changes, simultaneously imposed by carbonate in A and B sites of apatite","volume":"255","author":"Madupalli","year":"2017","journal-title":"J. Solid State Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.jobcr.2025.03.010","article-title":"Alginate-hydroxyapatite scaffolds: A comprehensive characterization study","volume":"15","author":"Gupta","year":"2025","journal-title":"J. Oral Biol. Craniofac. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.22146\/ijc.40907","article-title":"Extraction of hydroxyapatite from fish scales and its integration with rice husk for ammonia removal in aquaculture wastewater","volume":"19","author":"Hamzah","year":"2019","journal-title":"Indones. J. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"14635","DOI":"10.1021\/acs.analchem.1c02392","article-title":"Time-resolved ATR\u2013FTIR spectroscopy and macro ATR\u2013FTIR spectroscopic imaging of inorganic treatments for stone conservation","volume":"93","author":"Possenti","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jclepro.2012.11.001","article-title":"Biodiesel production from vegetable oil by using modified CaO as solid basic catalysts","volume":"42","author":"Tang","year":"2013","journal-title":"J. Clean. Prod."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1017\/S1431927621000301","article-title":"Structure and composition of the eggshell of a passerine bird, Setophaga ruticilla (Linnaeus, 1758)","volume":"27","author":"Dauphin","year":"2021","journal-title":"Microsc. Microanal."},{"key":"ref_65","first-page":"193","article-title":"Classification of fossil eggshells of amniotic vertebrates","volume":"36","author":"Mikhailov","year":"1991","journal-title":"Acta Palaeontol. Pol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"11857","DOI":"10.1038\/s41598-022-16033-5","article-title":"Analysis of ultrastructure and microstructure of blackbird (Turdus merula) and song thrush (Turdus philomelos) eggshell by scanning electron microscopy and X-ray computed microtomography","volume":"12","author":"Damaziak","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_67","unstructured":"Maleki, A. (2022). Identification and analytical methods. Heterogeneous Micro and Nanoscale Composites for the Catalysis of Organic Reactions, Elsevier."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"105939","DOI":"10.1016\/j.psj.2025.105939","article-title":"Proteomic changes in extracellular vesicle and uterine fluid proteins correlated with eggshell quality in aged laying hens","volume":"104","author":"Gao","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/BF02060296","article-title":"Ultrastructure and growth of the sea urchin tooth","volume":"14","author":"Kniprath","year":"1974","journal-title":"Calcif. Tissue Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1007\/BF00708280","article-title":"Structure of a sea urchin tooth","volume":"11","author":"Brear","year":"1976","journal-title":"J. Mater. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.geoderma.2013.08.015","article-title":"A new form of needle-fiber calcite produced by physical weathering of shells","volume":"213","author":"Villagran","year":"2014","journal-title":"Geoderma"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"101622","DOI":"10.1016\/j.psj.2021.101622","article-title":"Eggshell decalcification and skeletal mineralization during chicken embryonic development: Defining candidate genes in the chorioallantoic membrane","volume":"101","author":"Halgrain","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Chen, L., Wang, S., Feng, Y., Zhang, J., Du, Y., Zhang, J., Van Ongeval, C., Ni, Y., and Li, Y. (2021). Utilisation of chick embryo chorioallantoic membrane as a model platform for imaging-navigated biomedical research. Cells, 10.","DOI":"10.3390\/cells10020463"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.abb.2014.07.006","article-title":"Role of angiogenesis in bone repair","volume":"561","author":"Saran","year":"2014","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.3382\/ps.0681569","article-title":"Calcium reserve assembly: A basic structural unit of the calcium reserve system of the hen eggshell","volume":"68","author":"Deickert","year":"1989","journal-title":"Poult. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e35496","DOI":"10.1002\/jbm.b.35496","article-title":"Implementing chicken chorioallantoic membrane (CAM) assays for validating biomaterials in tissue engineering: Rationale and methods","volume":"112","author":"Dhayer","year":"2024","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Pham, H.M., and Tran, S.D. (2024). The chicken egg: An advanced material for tissue engineering. Biomolecules, 14.","DOI":"10.3390\/biom14040439"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Matuszewski, A., \u0141ukasiewicz, M., Niemiec, J., Kamaszewski, M., Jaworski, S., Domino, M., Jasi\u0144ski, T., Chwalibog, A., and Sawosz, E. (2021). Calcium carbonate nanoparticles\u2014Toxicity and effect of in ovo inoculation on chicken embryo development, broiler performance and bone status. Animals, 11.","DOI":"10.3390\/ani11040932"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"S83","DOI":"10.1016\/j.matpr.2020.06.100","article-title":"Synthesis of B-type carbonated hydroxyapatite by a new dissolution-precipitation method","volume":"31","author":"Benataya","year":"2020","journal-title":"Mater. Today Proc."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Ion, R.M., Iancu, L., Vasilievici, G., Grigore, M.E., Andrei, R.E., Radu, G.I., Grigorescu, R.M., Teodorescu, S., Bucurica, I.A., and Ion, M.L. (2019). Ion-substituted carbonated hydroxyapatite coatings for model stone samples. Coatings, 9.","DOI":"10.3390\/coatings9040231"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Campbell, S., Gao, B., and Poduska, K.M. (2020). Mid- and far-infrared spectral links for calcium carbonate polymorphs. Proceedings of the 2020 45th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz), IEEE.","DOI":"10.1109\/IRMMW-THz46771.2020.9370494"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"142","DOI":"10.17721\/fujcV10I1P142-154","article-title":"Removal of phosphates in aqueous solution by adsorption on calcium oxide","volume":"10","author":"Belaidouni","year":"2022","journal-title":"Fr.-Ukr. J. Chem."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1177\/0095244312452273","article-title":"Removal of water extractable proteins from concentrated natural rubber latex by eggshells","volume":"45","author":"Tangboriboon","year":"2013","journal-title":"J. Elastomers Plast."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"129","DOI":"10.21776\/ub.jpacr.2018.007.02.390","article-title":"Calcium oxide catalyst based on quail eggshell for biodiesel synthesis from waste palm oil","volume":"7","author":"Mohadi","year":"2018","journal-title":"J. Pure Appl. Chem. Res."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3023\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:22:35Z","timestamp":1790914955000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":84,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ani16193023"],"URL":"https:\/\/doi.org\/10.3390\/ani16193023","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]}}}