{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:55:02Z","timestamp":1792788902093,"version":"4.5.0"},"reference-count":49,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rufford Foundation","award":["49530-B (Booster)"],"award-info":[{"award-number":["49530-B (Booster)"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Rufford Foundation","award":["35454-1"],"award-info":[{"award-number":["35454-1"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Rufford Foundation","award":["43132-2"],"award-info":[{"award-number":["43132-2"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"crossref"}]},{"award":["49530-B (Booster)"],"award-info":[{"award-number":["49530-B (Booster)"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"publisher"}]},{"award":["35454-1"],"award-info":[{"award-number":["35454-1"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"publisher"}]},{"award":["43132-2"],"award-info":[{"award-number":["43132-2"]}],"id":[{"id":"https:\/\/ror.org\/02bxrrf91","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Indianapolis Zoo","award":["2025.WF.1"],"award-info":[{"award-number":["2025.WF.1"]}],"id":[{"id":"https:\/\/ror.org\/01k9v9b21","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2025.WF.1"],"award-info":[{"award-number":["2025.WF.1"]}],"id":[{"id":"https:\/\/ror.org\/01k9v9b21","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Mohamed bin Zayed Species Conservation Fund","award":["240534319"],"award-info":[{"award-number":["240534319"]}],"id":[{"id":"https:\/\/ror.org\/002skex07","id-type":"ROR","asserted-by":"crossref"}]},{"award":["240534319"],"award-info":[{"award-number":["240534319"]}],"id":[{"id":"https:\/\/ror.org\/002skex07","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China","award":["W2432021"],"award-info":[{"award-number":["W2432021"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"award":["W2432021"],"award-info":[{"award-number":["W2432021"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The Dehradun Stream Frog, Amolops chakrataensis, is a Critically Endangered stream-dwelling amphibian endemic to Uttarakhand in the western Himalayas of India and the species was previously known only from a single holotype collected in 1985. Despite repeated surveys, the species remained undetected for more than four decades, raising concerns that it went extinct. Here, we report the rediscovery of A. chakrataensis from the Maneri landscape, Uttarakhand, approximately 65 km on a straight-line east of the type locality. We confirmed the species identity using an integrative taxonomic approach combining morphological comparisons with the holotype, and we sequenced the mitochondrial 16S rRNA DNA gene fragment. Our analyses recovered A. chakrataensis as a distinct clade. Extensive field surveys covering 14 sites and 424 person-hours detected only two individuals, highlighting the rarity of the species. Using environmental characteristics of the three known localities, we developed a habitat envelope that identified additional priority areas for future surveys and potential conservation management. Our findings confirm the continued persistence of A. chakrataensis, provide the first molecular reference for the species, extend its known distribution, and establish an important foundation for future monitoring and conservation planning in the western Himalayas.<\/jats:p>","DOI":"10.3390\/ani16193072","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:14:37Z","timestamp":1790694877000},"page":"3072","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rediscovery of the Lost and Critically Endangered Dehradun Stream Frog, Amolops chakrataensis"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1818-0903","authenticated-orcid":false,"given":"Vishal Kumar","family":"Prasad","sequence":"first","affiliation":[{"name":"Laboratory of Animal Behaviour and Conservation, College of Ecology and Environment, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Wildlife Institute of India, Dehradun 248001, Uttarakhand, India"},{"name":"Amphibian Specialist Group, Species Survival Commission, International Union for the Conservation of Nature, 1196 Gland, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurav","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Wildlife Institute of India, Dehradun 248001, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4648-5608","authenticated-orcid":false,"given":"Sky T.","family":"Button","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Behaviour and Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5998-9445","authenticated-orcid":false,"given":"Sumbul","family":"Gill","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Behaviour and Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Badola","sequence":"additional","affiliation":[{"name":"Biodiversity Research and Conservation Foundation, Dehradun 248001, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devendra","family":"Singh","sequence":"additional","affiliation":[{"name":"Wildlife Institute of India, Dehradun 248001, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep Kumar","family":"Gupta","sequence":"additional","affiliation":[{"name":"Wildlife Institute of India, Dehradun 248001, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6700-4896","authenticated-orcid":false,"given":"K. P.","family":"Dinesh","sequence":"additional","affiliation":[{"name":"Zoological Survey of India, Western Regional Centre, Pune 411007, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1093-677X","authenticated-orcid":false,"given":"Ama\u00ebl","family":"Borz\u00e9e","sequence":"additional","affiliation":[{"name":"Amphibian Specialist Group, Species Survival Commission, International Union for the Conservation of Nature, 1196 Gland, Switzerland"},{"name":"Laboratory of Animal Behaviour and Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Department of Zoology and General Biology, Faculty of Life Sciences, Fergana State University, Fergana 150100, Uzbekistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1126\/science.1103538","article-title":"Status and trends of amphibian declines and extinctions worldwide","volume":"306","author":"Stuart","year":"2004","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1111\/geb.12571","article-title":"Amphibian sensitivity to habitat modification is associated with population trends and species traits","volume":"26","author":"Nowakowski","year":"2017","journal-title":"Glob. Ecol. Biogeogr."},{"key":"ref_3","unstructured":"IPCC (2023). Climate Change 2021: The Physical Science Basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change, Cambridge University Press. Available online: https:\/\/www.ipcc.ch\/report\/ar6\/wg1\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1038\/s41586-025-08665-0","article-title":"Vulnerability of amphibians to global warming","volume":"639","author":"Pottier","year":"2025","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1038\/s41558-026-02669-y","article-title":"Temperate local extinctions from climate change are outpacing tropical extinctions","volume":"16","author":"Murali","year":"2026","journal-title":"Nat. Clim. Chang."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Scheffers, B.R., Yong, D.L., Harris, J.B.C., Giam, X., and Sodhi, N.S. (2011). The world\u2019s rediscovered species: Back from the brink?. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022531"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1080\/19768354.2026.2614151","article-title":"From threatened to widespread: A non-genuine conservation status update for a now widespread Asian hylid","volume":"30","author":"Um","year":"2026","journal-title":"Anim. Cells Syst."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"127362","DOI":"10.1016\/j.jnc.2026.127362","article-title":"Rediscovery and conservation of two threatened Diospyros (Ebenaceae) species endemic to a single small island, last collected in 1961","volume":"83","author":"Nurdiana","year":"2026","journal-title":"J. Nat. Conserv."},{"key":"ref_9","first-page":"346","article-title":"Two new hill-stream frogs of the genus Amolops Cope (Amphibia: Anura: Ranidae) from Uttar Pradesh (India)","volume":"15","author":"Ray","year":"1992","journal-title":"Indian J. For."},{"key":"ref_10","unstructured":"IUCN SSC Amphibian Specialist Group (2026, July 07). Amolops chakrataensis. The IUCN Red List of Threatened Species 2023, e.T58203A166098755. Available online: https:\/\/www.iucnredlist.org\/species\/58203\/166098755."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"29098","DOI":"10.11609\/jott.10116.18.6.29098-29105","article-title":"Evidence for the local extirpation of the Dehradun stream frog Amolops chakrataensis Ray, 1992 from the type locality, Chakrata in the western Himalayas, India, and associated threats: A call for urgent conservation action","volume":"18","author":"Prasad","year":"2026","journal-title":"J. Threat. Taxa"},{"key":"ref_12","first-page":"1","article-title":"Systematic Studies on the Amphibian Fauna of the District Dehradun, Uttar Pradesh, India","volume":"18","author":"Ray","year":"1999","journal-title":"Mem. Zool. Surv. India"},{"key":"ref_13","unstructured":"Moore, R. (2014). In Search of Lost Frogs, Bloomsbury Publishing."},{"key":"ref_14","unstructured":"Ray, P., and Dutta, S. (2026, July 07). Amolops chakrataensis. The IUCN Red List of Threatened Species 2004, e.T58203A11745673. Available online: https:\/\/www.iucnredlist.org\/species\/58203\/11745673."},{"key":"ref_15","first-page":"142","article-title":"A review of torrent frogs (Amolops: Ranidae) from Bhutan, the description of a new species, and reassessment of the taxonomic validity of some A. viridimaculatus group species aided by archival DNA sequences of century-old type specimens","volume":"35","author":"Mahony","year":"2022","journal-title":"Herpetozoa"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e3872","DOI":"10.7717\/peerj.3872","article-title":"Impact of land reclamation and agricultural water regime on the distribution and conservation status of the endangered Dryophytes suweonensis","volume":"5","author":"Kim","year":"2017","journal-title":"PeerJ"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e5568","DOI":"10.7717\/peerj.5568","article-title":"Population trend inferred from aural surveys for calling anurans in Korea","volume":"6","author":"Andersen","year":"2018","journal-title":"PeerJ"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"450","DOI":"10.11646\/zootaxa.4851.3.2","article-title":"Identification of anuran species diversity of the Panna Tiger Reserve, Central India, using an integrated approach","volume":"4851","author":"Prasad","year":"2020","journal-title":"Zootaxa"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e70294","DOI":"10.1002\/ece3.70294","article-title":"Optimising recovery of DNA from minimally invasive sampling methods: Efficacy of buccal swabs, preservation strategy and DNA extraction approaches for amphibian studies","volume":"14","author":"Martin","year":"2024","journal-title":"Ecol. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wren, S., Borz\u00e9e, A. (2024). Infectious diseases: Advances and challenges in addressing emerging pathogens. Amphibian Conservation Action Plan: A Status Review and Roadmap for Global Amphibian Conservation, IUCN SSC Amphibian Specialist Group. Available online: https:\/\/portals.iucn.org\/library\/sites\/library\/files\/documents\/SSC-OP-057-En.pdf.","DOI":"10.2305\/QWVH2717"},{"key":"ref_21","unstructured":"IUCN Species Survival Commission (2013). Guidelines for Reintroductions and Other Conservation Translocations, Version 1.0, IUCN Species Survival Commission. Available online: https:\/\/portals.iucn.org\/library\/node\/10386."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"36","DOI":"10.61186\/JAD.5.1.36","article-title":"Description of a new species of Amolops (Anura: Ranidae) from a cave ecosystem in Meghalaya, Northeast India","volume":"5","author":"Saikia","year":"2023","journal-title":"J. Anim. Divers."},{"key":"ref_23","unstructured":"Frost, D.R. (2026, July 05). Amphibian Species of the World: An Online Reference, Version 6.2; American Museum of Natural History: New York, NY, USA, 2026. Available online: https:\/\/amphibiansoftheworld.amnh.org."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1139\/g93-141","article-title":"The mitochondrial genome of Anopheles quadrimaculatus species A: Complete nucleotide sequence and gene organization","volume":"36","author":"Mitchell","year":"1993","journal-title":"Genome"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_26","first-page":"772","article-title":"PartitionFinder 2: New methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses","volume":"34","author":"Lanfear","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lanfear, R., Calcott, B., Kainer, D., Mayer, C., and Stamatakis, A. (2014). Selecting optimal partitioning schemes for phylogenomic datasets. BMC Evol. Biol., 14.","DOI":"10.1186\/1471-2148-14-82"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/sysbio\/syq010","article-title":"New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0","volume":"59","author":"Guindon","year":"2010","journal-title":"Syst. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1093\/sysbio\/sys029","article-title":"MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space","volume":"61","author":"Ronquist","year":"2012","journal-title":"Syst. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1093\/sysbio\/syy032","article-title":"Posterior summarisation in Bayesian phylogenetics using Tracer 1.7","volume":"67","author":"Rambaut","year":"2018","journal-title":"Syst. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e07294","DOI":"10.1111\/ecog.07294","article-title":"Optimising occurrence data in species distribution models: Sample size, positional uncertainty, and sampling bias matter","volume":"2024","author":"Bazzichetto","year":"2024","journal-title":"Ecography"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1890\/06-1312.1","article-title":"Habitat classification modeling with incomplete data: Pushing the habitat envelope","volume":"17","author":"Zarnetske","year":"2007","journal-title":"Ecol. Appl."},{"key":"ref_34","unstructured":"Bourgoin, C., Verhegghen, A., Ameztoy, I., Carboni, S., Achard, F., and Colditz, R. (2026). Global Map of Forest Cover 2020\u2014Version 3, European Commission, Joint Research Centre."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"Q12004","DOI":"10.1029\/2012GC004370","article-title":"The new global lithological map database GLiM: A representation of rock properties at the Earth surface","volume":"13","author":"Hartmann","year":"2012","journal-title":"Geochem. Geophys. Geosyst."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1080\/00222933.2021.1946185","article-title":"Phylogenetic position of the poorly known montane cascade frog Amolops monticola (Ranidae) and description of a new closely related species from Northeast India","volume":"55","author":"Patel","year":"2021","journal-title":"J. Nat. Hist."},{"key":"ref_37","unstructured":"GBIF.org (2026, June 02). GBIF Occurrence Download. Available online: https:\/\/doi.org\/10.15468\/dL.uxb94k."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ympev.2006.11.032","article-title":"Phylogeny of Raninae (Anura: Ranidae) inferred from mitochondrial and nuclear sequences","volume":"43","author":"Che","year":"2007","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"31","DOI":"10.11646\/zootaxa.2408.1.2","article-title":"Description of two new species of torrent frog, Amolops Cope (Anura: Ranidae) from a degrading forest in the northeast Indian state of Nagaland","volume":"2408","author":"Biju","year":"2010","journal-title":"Zootaxa"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1755-263X.2010.00158.x","article-title":"\u201cSight-unseen\u201d detection of rare aquatic species using environmental DNA","volume":"4","author":"Jerde","year":"2011","journal-title":"Conserv. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5624","DOI":"10.1038\/s41598-022-09084-1","article-title":"Development and validation of an eDNA protocol for monitoring endemic Asian spiny frogs in the Himalayan region of Pakistan","volume":"12","author":"Saeed","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"852","DOI":"10.15560\/21.5.852","article-title":"Atelopus marinkellei Cochran & Goin, 1970 (Amphibia, Anura, Bufonidae): After 20 years, the rediscovery of a Critically Endangered species in the p\u00e1ramo of Siscuns\u00ed, a Neotropical high-mountain ecosystem","volume":"21","year":"2025","journal-title":"Check List"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s00227-025-04679-4","article-title":"Rediscovery of Rhizopsammia wellingtoni in Gal\u00e1pagos after two decades","volume":"172","author":"Keith","year":"2025","journal-title":"Mar. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00114-026-02074-7","article-title":"Genetic evidence for the rediscovery in the wild of the critically endangered Sahara killifish Apricaphanius saourensis (Cyprinodontiformes: Aphaniidae)","volume":"113","author":"Derouiche","year":"2026","journal-title":"Sci. Nat."},{"key":"ref_45","first-page":"345","article-title":"Impact of climate change on the western Himalayan Mountain ecosystems: An overview","volume":"53","author":"Negi","year":"2012","journal-title":"Trop. Ecol."},{"key":"ref_46","unstructured":"Singh, H. (2024). Climate-induced changes in forest ecosystems of the Indian Himalayan Region: A meta-analysis. Urban Forests, Climate Change and Environmental Pollution, Springer."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wren, S., and Borz\u00e9e, A. (2024). Overview of amphibians and their conservation. Amphibian Conservation Action Plan: A Status Review and Roadmap for Global Amphibian Conservation, IUCN SSC Amphibian Specialist Group. Available online: https:\/\/portals.iucn.org\/library\/sites\/library\/files\/documents\/SSC-OP-057-En.pdf.","DOI":"10.2305\/QWVH2717"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wren, S., and Borz\u00e9e, A. (2024). Communications and education for amphibian conservation. Amphibian Conservation Action Plan: A Status Review and Roadmap for Global Amphibian Conservation, IUCN SSC Amphibian Specialist Group. Available online: https:\/\/portals.iucn.org\/library\/sites\/library\/files\/documents\/SSC-OP-057-En.pdf.","DOI":"10.2305\/QWVH2717"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/s44358-025-00101-5","article-title":"Conservation priorities for global amphibian biodiversity","volume":"1","author":"Prasad","year":"2025","journal-title":"Nat. Rev. Biodivers."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3072\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:15:34Z","timestamp":1790828134000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3072"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":49,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ani16193072"],"URL":"https:\/\/doi.org\/10.3390\/ani16193072","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]}}}