{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:49:27Z","timestamp":1791607767377,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["2023TD81"],"award-info":[{"award-number":["2023TD81"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["20603022025013"],"award-info":[{"award-number":["20603022025013"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["BRESG-JB202518"],"award-info":[{"award-number":["BRESG-JB202518"]}]},{"name":"Qingdao Postdoctoral Science Foundation","award":["QDBSH20240102012"],"award-info":[{"award-number":["QDBSH20240102012"]}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2025QC239"],"award-info":[{"award-number":["ZR2025QC239"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Dissolved oxygen (DO) is a key environmental factor affecting the survival and development of farmed fish. However, the hypoxia responses of white trevally (Pseudocaranx dentex) remain poorly understood. In this study, we determined the DO tolerance thresholds and integrated physiological, histological, and molecular responses of white trevally to hypoxic stress. Smaller fish exhibited higher values for critical oxygen tension (3.50 \u00b1 0.18 mg\/L) and loss of equilibrium (LOE, 0.92 \u00b1 0.14 mg\/L), whereas larger fish showed lower values. Hypoxia significantly elevated serum cortisol, glucose, alanine aminotransferase, and aspartate aminotransferase levels, which peaked at LOE. Under hypoxia, histological analysis revealed lamellar clubbing, hypertrophy, and hyperplasia in gills, and vacuolization and nuclear migration in liver sections. In the liver, superoxide dismutase and catalase activities and malondialdehyde content increased, while glutathione peroxidase activity decreased. Upregulation was observed for stress-related genes (HSP70, HSP90, mineralocorticoid receptor, and glucocorticoid receptor) and pro-apoptotic genes (p53, BAX, and Caspase-3), while Bcl-2 was downregulated. Principal component analysis indicated that hypoxia triggered stress responses, tissue damage and apoptotic signaling; smaller fish were the most vulnerable, emphasizing the need for size-specific management in aquaculture. Collectively, these findings provide baseline data that may support the healthy development of traditional white trevally aquaculture.<\/jats:p>","DOI":"10.3390\/ani16193137","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T10:13:02Z","timestamp":1791367982000},"page":"3137","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physiological, Histological, and Molecular Responses of White Trevally (Pseudocaranx dentex) to Acute Hypoxia"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7651-1718","authenticated-orcid":false,"given":"Xiatian","family":"Chen","sequence":"first","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialing","family":"Luo","sequence":"additional","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenglin","family":"Wang","sequence":"additional","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yudong","family":"Jia","sequence":"additional","affiliation":[{"name":"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"740672","DOI":"10.1016\/j.aquaculture.2024.740672","article-title":"Different dissolved oxygen effects on growth and O2-sensing pathway in juvenile bighead carp (Hypophthalmichthys nobilis)","volume":"584","author":"Zhou","year":"2024","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e12985","DOI":"10.1111\/raq.12985","article-title":"Water Quality Impact on Fish Behavior: A Review From an Aquaculture Perspective","volume":"17","author":"Zhang","year":"2025","journal-title":"Rev. Aquac."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e70111","DOI":"10.1111\/jwas.70111","article-title":"Impact of diet, dissolved oxygen concentration, and stocking density on channel catfish (Ictalurus punctatus) production performance","volume":"57","author":"Ott","year":"2026","journal-title":"J. World Aquac. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"738673","DOI":"10.1016\/j.aquaculture.2022.738673","article-title":"Effects of chronic hypoxia on growth performance, antioxidant capacity and protein turnover of largemouth bass (Micropterus salmoides)","volume":"561","author":"He","year":"2022","journal-title":"Aquaculture"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"743043","DOI":"10.1016\/j.aquaculture.2025.743043","article-title":"On-growing Atlantic salmon (Salmo salar L.) acclimate behaviourally and physiologically to chronic hypoxia, but exhibit reduced feed intake, growth and lipid retention","volume":"611","author":"Liland","year":"2026","journal-title":"Aquaculture"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"coae066","DOI":"10.1093\/conphys\/coae066","article-title":"Multiple stressors disrupt sex hormones and fitness outcomes: Effects of hypoxia and turbidity on an African cichlid fish","volume":"12","author":"Williams","year":"2024","journal-title":"Conserv. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sato, E., Shimamura, N., Saiki, C., Sunada, K., Miwa, N., and Xiao, L. (2025). Hydrogen Gas Mitigates Acute Hypoxia-Induced Oxidative and Inflammatory Brain Injuries in Medaka (Oryzias latipes). Antioxidants, 14.","DOI":"10.3390\/antiox14091130"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s40104-025-01274-x","article-title":"Glutathione mitigates hypoxia-induced gill damage in juvenile grass carp (Ctenopharyngodon idellus) by alleviating endoplasmic reticulum stress and autophagy","volume":"16","author":"Zeng","year":"2025","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"110127","DOI":"10.1016\/j.compag.2025.110127","article-title":"Hypoxia monitoring of fish in intensive aquaculture based on underwater multi-target tracking","volume":"232","author":"Li","year":"2025","journal-title":"Comput. Electron. Agric."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"111544","DOI":"10.1016\/j.fsi.2026.111544","article-title":"Autochthonous probiotic Lactococcus lactis MA5 improves recovery from acute hypoxia stress and resistance to Edwardsiella ictaluri in hybrid catfish (Ictalurus punctatus \u00d7 I. furcatus)","volume":"176","author":"Huang","year":"2026","journal-title":"Fish Shellfish Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.aquaculture.2018.05.023","article-title":"Quality parameters of wild white trevally (Pseudocaranx dentex) natural spawn kept in captivity","volume":"495","author":"Nogueira","year":"2018","journal-title":"Aquaculture"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0044-8486(03)00494-0","article-title":"Broodstock nutrition research on marine finfish in Japan","volume":"227","author":"Watanabe","year":"2003","journal-title":"Aquaculture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.1111\/are.15192","article-title":"Suitable light intensity during the ontogenetic development of white trevally, Pseudocaranx dentex (Bloch and Schneider, 1801), larvae","volume":"52","author":"Honryo","year":"2021","journal-title":"Aquac. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e12720","DOI":"10.7717\/peerj.12720","article-title":"Expression profiles and transcript properties of fast-twitch and slow-twitch muscles in a deep-sea highly migratory fish, Pseudocaranx dentex","volume":"10","author":"Wang","year":"2022","journal-title":"Peerj"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Li, B.S., Wang, H., Yang, L., Liu, S.F., and Zhuang, Z.M. (2021). Complete Mitochondrial Genome of Pseudocaranx dentex (Carangidae, Perciformes) Provides Insight into Phylogenetic and Evolutionary Relationship among Carangidae Family. Genes, 12.","DOI":"10.3390\/genes12081234"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wang, H., Li, B.S., Li, J.F., Jiang, C., Liu, S.F., and Zhuang, Z.M. (2022). Label-Free Quantitative Proteomic Analysis Provides Insight Into the Differences Between Slow-Twitch Muscle and Fast-Twitch Muscle of Pseudocaranx dentex. Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.842172"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"144156","DOI":"10.1016\/j.ijbiomac.2025.144156","article-title":"Transcriptome profiling and alternative splicing analysis of skeletal muscle in Pseudocaranx dentex: Insights into slow-twitch and fast-twitch muscle specialization","volume":"312","author":"Wang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wang, H., Li, B.S., Li, A., An, C.T., Liu, S.F., and Zhuang, Z.M. (2024). Integrative Metabolomics, Enzymatic Activity, and Gene Expression Analysis Provide Insights into the Metabolic Profile Differences between the Slow-Twitch Muscle and Fast-Twitch Muscle of Pseudocaranx dentex. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25116131"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3147\/jsfp.59.119","article-title":"Characterization and Epidemiological Study of Newly Emerging Lactococcus garvieae Serotype III in Farmed Fish in Japan","volume":"59","author":"Araki","year":"2024","journal-title":"Fish. Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"103146","DOI":"10.1016\/j.parint.2025.103146","article-title":"Morphological and molecular characterization of four Ceratomyxa species parasitizing marine fish in Japan including descriptions of three new species","volume":"110","author":"Oe","year":"2026","journal-title":"Parasitol. Int."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Li, B.S., Wang, H., Li, A., An, C.T., Zhu, L., Liu, S.F., and Zhuang, Z.M. (2022). The Landscape of DNA Methylation Generates Insight Into Epigenetic Regulation of Differences Between Slow-Twitch and Fast-Twitch Muscles in Pseudocaranx dentex. Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.916373"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s10126-025-10550-w","article-title":"Functional Roles and Regulatory Factors of Histidine-Related Compounds in Skeletal Muscle Metabolism of Teleosts with Distinct Swimming Strategies","volume":"28","author":"Wang","year":"2025","journal-title":"Mar. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"736380","DOI":"10.1016\/j.aquaculture.2021.736380","article-title":"Hypoxia tolerance, hematological, and biochemical response in juvenile turbot (Scophthalmus maximus. L)","volume":"535","author":"Jia","year":"2021","journal-title":"Aquaculture"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s10695-025-01471-5","article-title":"Hypoxia tolerance and physiological coping strategies in fat greenling (Hexagrammos otakii)","volume":"51","author":"Wang","year":"2025","journal-title":"Fish Physiol. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5695","DOI":"10.1111\/gcb.16319","article-title":"Body mass and cell size shape the tolerance of fishes to low oxygen in a temperature-dependent manner","volume":"28","author":"Verberk","year":"2022","journal-title":"Glob. Change Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"coac087","DOI":"10.1093\/conphys\/coac087","article-title":"Body size dictates physiological and behavioural responses to hypoxia and elevated water temperatures in Murray cod (Maccullochella peelii)","volume":"11","author":"McPhee","year":"2023","journal-title":"Conserv. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"eadt7079","DOI":"10.1126\/sciadv.adt7079","article-title":"Individual clown anemonefish shrink to survive heat stress and social conflict","volume":"11","author":"Versteeg","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jahafs\/vsae002","article-title":"Fingerling stocking size has no influence on proliferative gill disease severity in farm-raised Channel Catfish","volume":"37","author":"Richardson","year":"2025","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1093\/icb\/icae094","article-title":"Energetic Costs of Stress in Developing Fishes: Quantifying Allostasis and Allostatic Load","volume":"64","year":"2024","journal-title":"Integr. Comp. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e005368","DOI":"10.1136\/bmjdrc-2025-005368","article-title":"Associations between perceived stress and glycemic measures: Gender and age as moderators","volume":"13","author":"Tsai","year":"2025","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4833","DOI":"10.1021\/acssuschemeng.4c10847","article-title":"Simulated Environmental Nanoplastics Induce Zebrafish Developmental Toxicity and Stress Response","volume":"13","author":"Saraceni","year":"2025","journal-title":"Acs Sustain. Chem. Eng."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"744325","DOI":"10.1016\/j.aquaculture.2026.744325","article-title":"Size-dependent salinity tolerance in juvenile stellate sturgeon (Acipenser stellatus): Physiological and histopathological responses","volume":"625","author":"Pajand","year":"2026","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"110584","DOI":"10.1016\/j.fsi.2025.110584","article-title":"Single-cell RNA sequencing reveals heterogeneous response of interlamellar cell mass to hypoxic stress in crucian carp (Carassius auratus) gills","volume":"166","author":"Wu","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"106502","DOI":"10.1016\/j.aquatox.2023.106502","article-title":"Hypoxia stress induces hepatic antioxidant activity and apoptosis, but stimulates immune response and immune-related gene expression in black rockfish Sebastes schlegelii","volume":"258","author":"Jia","year":"2023","journal-title":"Aquat. Toxicol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"17934","DOI":"10.1038\/s41598-023-45103-5","article-title":"Physiological and biochemical mechanisms of grain yield loss in fumitory (Fumaria parviflora Lam.) exposed to copper and drought stress","volume":"13","author":"Tashakorizadeh","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Amaral, D., Filipe, D.M., Cavalheri, T.F., Vieira, L., Magalh\u00e3es, R.P., Belo, I., Peres, H., and Oz\u00f3rio, R.O.d.A. (2023). Solid-State Fermentation of Plant Feedstuff Mixture Affected the Physiological Responses of European Seabass (Dicentrarchus labrax) Reared at Different Temperatures and Subjected to Salinity Oscillation. Animals, 13.","DOI":"10.3390\/ani13030393"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9406490","DOI":"10.1155\/anu\/9406490","article-title":"Branched-Chain Amino Acids Target miR-203a\/fosb Axis to Promote Skeletal Muscle Growth in Common Carp (Cyprinus carpio)","volume":"2025","author":"Cao","year":"2025","journal-title":"Aquac. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9000","DOI":"10.1038\/s41467-024-52942-x","article-title":"Mechanisms of epigenomic and functional convergence between glucocorticoid- and IL4-driven macrophage programming","volume":"15","author":"Deochand","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"740444","DOI":"10.1016\/j.aquaculture.2023.740444","article-title":"Effects of hypoxia stress on oxidative stress, apoptosis and microorganisms in the intestine of large yellow croaker (Larimichthys crocea)","volume":"581","author":"Liu","year":"2024","journal-title":"Aquaculture"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s40104-024-01089-2","article-title":"Curcumin attenuates ochratoxin A and hypoxia co-induced liver injury in grass carp (Ctenopharyngodon idella) by dual targeting endoplasmic reticulum stress and apoptosis via reducing ROS content","volume":"15","author":"Wu","year":"2024","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"741081","DOI":"10.1016\/j.aquaculture.2024.741081","article-title":"Modulation of chronic hypoxia on ovarian structure, oxidative stress, and apoptosis in female Nile Tilapia (Oreochromis niloticus)","volume":"590","author":"Ali","year":"2024","journal-title":"Aquaculture"}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3137\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:12:32Z","timestamp":1791605552000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/16\/19\/3137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":41,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ani16193137"],"URL":"https:\/\/doi.org\/10.3390\/ani16193137","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]}}}