{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T22:56:51Z","timestamp":1780095411917,"version":"3.54.0"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T00:00:00Z","timestamp":1548201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31402111"],"award-info":[{"award-number":["31402111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The objective of this study was to evaluate the effects of dietary inclusion of stevioside on feed intake, feeding behavior, nutrient digestion, rumen fermentation, and serum biochemical parameters in goats. Nine male Xiangdong black goats (21.8 \u00b1 1.5 kg of body weight) were used in a replicated 3 \u00d7 3 Latin square. All goats were fed a basal diet including concentrate and forage (chopped rice straw). The three treatments were 0, 400, or 800 mg stevioside per kg chopped rice straw on a dry matter (DM) basis. Dry matter intake of forage and total diet linearly increased (p = 0.03 and p = 0.04) with increasing stevioside in the diet. There was no effect (p &gt; 0.05) of stevioside inclusion in the diets on eating time, rumination time, or total mastication time for the goats. Total volatile fatty acid (VFA) concentration in the rumen responded quadratically (p &lt; 0.01), decreasing from 0 to 400 mg\/kg stevioside inclusion and increasing thereafter. The inclusion of steviosid in the diets promoted a quadratic increase in the apparent total tract digestibilities of neutral detergent fiber (NDF) (p = 0.02) and acid detergent fiber (ADF) (p = 0.01). Based on the results of this experiment, it could be concluded that supplementing goat diets with stevioside at 400 mg\/kg to 800 mg\/kg forage (about 270 to 541 mg\/kg diet) resulted in increased dry intake of forage and total diet, suggesting that stevioside has positive potential as a feed additive to improve feed intake.<\/jats:p>","DOI":"10.3390\/ani9020032","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T03:52:32Z","timestamp":1548301952000},"page":"32","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Effects of Dietary Stevioside Supplementation on Feed Intake, Digestion, Ruminal Fermentation, and Blood Metabolites of Goats"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1427-5198","authenticated-orcid":false,"given":"Xuefeng","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-859X","authenticated-orcid":false,"given":"Chaoxi","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"},{"name":"College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoxun","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiurong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"},{"name":"Hunan Co-Innovation Center of Animal Production Safety (CICAPS), Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, The Chinese Academy of Sciences (CAS), Changsha 410125, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1051\/animres:19960501","article-title":"Palatability and feeding behaviour in ruminants. A review","volume":"45","author":"Baumont","year":"1996","journal-title":"Ann. Zootech."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1079\/BJN19930007","article-title":"Factors affecting the voluntary intake of food by sheep 6. the effect of monosodium glutamate on the palatability of straw diets by sham-fed and normal animals","volume":"69","author":"Colucci","year":"1993","journal-title":"Br. J. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1071\/AR9800571","article-title":"The influence of odor and taste on the food preferences and food-intake of sheep","volume":"31","author":"Arnold","year":"1980","journal-title":"Aust. J. Agric. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.2527\/jas.2006-098","article-title":"Effects of an artificial sweetener on health, performance, and dietary preference of feedlot cattle","volume":"84","author":"McMeniman","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.2527\/jas.2013-6795","article-title":"Effects of a dietary sweetener on growth performance and health of stressed beef calves and on diet digestibility and plasma and urinary metabolite concentrations of healthy calves","volume":"92","author":"Ponce","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"364","DOI":"10.2527\/jas1970.312364x","article-title":"Taste responses in ruminants 3. reactions of pygmy goats, normal goats, sheep and cattle to sucrose and sodium chloride","volume":"31","author":"Goatcher","year":"1970","journal-title":"J. Anim. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.anifeedsci.2013.05.015","article-title":"Intake in cattle of a ground switchgrass and alfalfa hay mixture blended with various levels of sucrose or citric acid","volume":"184","author":"Chavez","year":"2013","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/09637480903193049","article-title":"Stevia (Stevia rebaudiana) a bio-sweetener: A review","volume":"61","author":"Goyal","year":"2010","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/j.biotechadv.2011.06.006","article-title":"Downstream processing of stevioside and its potential applications","volume":"29","author":"Puri","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"ref_10","first-page":"529","article-title":"Effects of a new sweetener, Stevia, on performance of newly weaned pips. Can","volume":"80","author":"Munro","year":"2000","journal-title":"J. Anim. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1016\/S0278-6915(03)00191-1","article-title":"Metabolism of stevioside in pigs and intestinal absorption characteristics of stevioside, rebaudioside A and steviol","volume":"41","author":"Geuns","year":"2003","journal-title":"Food. Chem. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.anifeedsci.2013.10.014","article-title":"Effects of dietary stevioside\/rebaudioside A on the growth performance and diarrhea incidence of weaned piglets","volume":"187","author":"Wang","year":"2014","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1021\/jf020835o","article-title":"Metabolism of stevioside by chickens","volume":"51","author":"Geuns","year":"2003","journal-title":"J. Agric. Food. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/j.1439-0396.2007.00760.x","article-title":"Evaluation of supplementary stevia (Stevia rebaudiana, bertoni) leaves and stevioside in broiler diets: Effects on feed intake, nutrient metabolism, blood parameters and growth performance","volume":"92","author":"Atteh","year":"2008","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.5713\/ajas.2014.14562","article-title":"The influence of feed energy density and a formulated additive on rumen and rectal temperature in hanwoo steers","volume":"27","author":"Cho","year":"2014","journal-title":"Asian Australas. J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0921-4488(02)00013-5","article-title":"Nutrient intake, digestibility, mastication and ruminal fermentation of lambs fed finishing ration with various forage levels","volume":"43","author":"Fimbres","year":"2002","journal-title":"Small Ruminant. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.3168\/jds.S0022-0302(02)74179-9","article-title":"Effect of concentrate level and feeding management on chewing activities, saliva production, and ruminal pH of lactating dairy cows","volume":"85","author":"Maekawa","year":"2002","journal-title":"J. Dairy Sci."},{"key":"ref_18","first-page":"27","article-title":"Perioperative ruminal pH changes in domestic sheep (Ovis aries) housed in a biomedical research setting","volume":"50","author":"Jasmin","year":"2011","journal-title":"J. Am. Assoc. Lab. Anim. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.2527\/jas.2015-9658","article-title":"Sampling procedure for the measurement of dissolved hydrogen and volatile fatty acids in the rumen of dairy cows","volume":"94","author":"Wang","year":"2016","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wu, D.Q., Xu, L.W., Tang, S.X., Guan, L.L., He, Z.X., Guan, Y.J., Tan, Z.L., Han, X.F., Zhou, C.S., and Kang, J.H. (2016). Influence of oleic acid on rumen fermentation and atty acid formation in vitro. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156835"},{"key":"ref_21","unstructured":"The Association Of Analytical Communities (AOAC) (1990). Official Methods of Analysis, AOAC. [15th ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","article-title":"Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition","volume":"74","author":"Robertson","year":"1991","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1021\/ac60252a045","article-title":"Phenol-hypochlorite reaction for determination of ammonia","volume":"39","author":"Weatherburn","year":"1967","journal-title":"Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2014.04.012","article-title":"Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures","volume":"194","author":"Wang","year":"2014","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_25","unstructured":"SAS Institute (2000). SAS\/STAT User\u2019s Guide. Release 8.02, SAS Institute Inc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/j.1348-0421.1997.tb01961.x","article-title":"Bactericidal activity of a fermented hot-water extract from Stevia rebaudiana Bertoni towards enterohemorrhagic Escherichia coli O157:H7 and other food-borne pathogenic bacteria","volume":"41","author":"Tomita","year":"1997","journal-title":"Microbiol. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0166-3542(00)00134-0","article-title":"Analysis of anti-rotavirus activity of extract from Stevia rebaudiana","volume":"49","author":"Takahashi","year":"2001","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/elsc.201000142","article-title":"Antibacterial activity of stevioside towards food-borne pathogenic bacteria","volume":"11","author":"Puri","year":"2011","journal-title":"Eng. Life Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.2527\/jas1985.6161559x","article-title":"Influence of forage level on passage rate, digestibility and performance of cattle","volume":"61","author":"Ledoux","year":"1985","journal-title":"J. Anim. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0377-8401(94)00700-J","article-title":"Ruminal plant cell wall digestibility estimated from digestion and passage kinetics utilizing mathematical models","volume":"52","author":"Huhtanen","year":"1995","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0377-8401(94)00699-A","article-title":"Comparison of methods, markers, sampling sites and models for estimating digesta passage kinetics in cattle fed at two levels of intake","volume":"52","author":"Huhtanen","year":"1995","journal-title":"Anim. Feed Sci. Technol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/2\/32\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:28:11Z","timestamp":1760185691000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/2\/32"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,23]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["ani9020032"],"URL":"https:\/\/doi.org\/10.3390\/ani9020032","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,23]]}}}