{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:48:13Z","timestamp":1778809693398,"version":"3.51.4"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:00:00Z","timestamp":1557187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The study proposed an exploratory functional analysis on differential gene expression of the jejunum and of cecum in chickens. For this study, 150 Ross 308 male chickens were randomly allotted in six pens (25 birds\/pen) and fed the same commercial diet. From 19 birds of 42 days of age, jejunum and cecum mucosae were collected for RNA extraction for transcriptome microarray analysis. Differentially expressed genes (DEGs) submitted to DAVID (Database for Annotation, Visualization, and Integrated Discovery) and Gene Set Enrichment Analysis (GSEA) software evidenced enriched gene clusters for biological functions differentiated in the tissues. DAVID analysis in the jejunum showed enriched annotations for cell membrane integral components, PPAR (peroxisome proliferator-activated receptor) signaling pathway, and peroxisome and lipid metabolism, and showed DEGs for gluconeogenesis, not previously reported in chicken jejunum. The cecum showed enriched annotations for disulfide bond category, cysteine and methionine metabolism, glycoprotein category, cell cycle, and extracellular matrix (ECM). GSEA analysis in the jejunum showed peroxisome and PPAR signaling pathway-related gene sets, as found with DAVID, and gene sets for immune regulation, tryptophan and histidine metabolism, and renin\u2013angiotensin system, like in mammals. The cecum showed cell cycle and regulation processes, as well as ECM receptor interaction and focal adhesion-related gene sets. Typical intestinal functions specific for the gut site and interesting functional genes groups emerged, revealing tissue-related key aspects which future studies might take advantage of.<\/jats:p>","DOI":"10.3390\/ani9050221","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T11:22:35Z","timestamp":1557400955000},"page":"221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Exploring Differential Transcriptome between Jejunal and Cecal Tissue of Broiler Chickens"],"prefix":"10.3390","volume":"9","author":[{"given":"Micol","family":"Bertocchi","sequence":"first","affiliation":[{"name":"Department of Agricultural and Food Sciences (DISTAL), University of Bologna, 40126 Bologna BO, Italy"},{"name":"Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Sirri","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences (DISTAL), University of Bologna, 40126 Bologna BO, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6583-3482","authenticated-orcid":false,"given":"Orazio","family":"Palumbo","sequence":"additional","affiliation":[{"name":"Division of Medical Genetics, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo FG, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Luise","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences (DISTAL), University of Bologna, 40126 Bologna BO, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Maiorano","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso CB, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Bosi","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences (DISTAL), University of Bologna, 40126 Bologna BO, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7019-6828","authenticated-orcid":false,"given":"Paolo","family":"Trevisi","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Food Sciences (DISTAL), University of Bologna, 40126 Bologna BO, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0168-9525(02)00014-8","article-title":"Life cycles of successful genes","volume":"19","author":"Hoffmann","year":"2003","journal-title":"Trends Genet."},{"key":"ref_2","unstructured":"Nature Briefing (2018, September 19). 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