{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:39:41Z","timestamp":1760240381777,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Discipline Construction Fund Project of Gansu Agricultural University","award":["GSAU-XKJS-2018-041"],"award-info":[{"award-number":["GSAU-XKJS-2018-041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>C. perfringens type C can induce enteritis accompanied by diarrhea and annually causes significant economic losses to the global pig industry. The pathogenic mechanisms of C. perfringens type C in pigs are still largely unknown. To investigate this, we challenged seven-day-old piglets with C. perfringens type C to cause diarrhea. We performed hematoxylin &amp; eosin (H&amp;E) staining of the small intestine (including duodenum, jejunum, and ileum) and assessed gene expression in the ileal tissue. H&amp;E staining of the duodenum, jejunum, and ileum demonstrated inflammation and edema of the lamina propria and submucosa. A total of 2181 differentially expressed genes (DEGs) were obtained in ileal tissues. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis of DEGs indicated that the main pathways were enriched in the T cell receptor signaling pathway, NF-kappa B signaling pathway, and (tumor necrosis factor) TNF signaling pathway. These results provide insights into the pathogenicity of C. perfringens type C and improve our understanding of host\u2013bacteria interactions.<\/jats:p>","DOI":"10.3390\/ani9050269","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T02:22:00Z","timestamp":1558664520000},"page":"269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Histological and Comparative Transcriptome Analyses Provide Insights into Small Intestine Health in Diarrheal Piglets after Infection with Clostridium Perfringens Type C"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8701-0647","authenticated-orcid":false,"given":"Zunqiang","family":"Yan","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouhu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoli","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiantuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Gansu Research Center for Swine Production Engineering and Technology, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangbao","family":"Gun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Gansu Research Center for Swine Production Engineering and Technology, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/MMBR.00062-12","article-title":"Toxin plasmids of Clostridium perfringens","volume":"77","author":"Li","year":"2013","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1177\/104063870501700602","article-title":"Clostridial enteric infections in pigs","volume":"17","author":"Songer","year":"2005","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_3","first-page":"219","article-title":"Clostridial diseases of small ruminants","volume":"29","author":"Songer","year":"1998","journal-title":"Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetmic.2011.02.048","article-title":"Recent progress in understanding the pathogenesis of Clostridium perfringens type C infections","volume":"153","author":"Uzal","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S0966-842X(98)01430-9","article-title":"Clostridium perfringens: Toxinotype and genotype","volume":"7","author":"Petit","year":"1999","journal-title":"Trends Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1128\/CMR.9.2.216","article-title":"Clostridial enteric diseases of domestic animals","volume":"9","author":"Songer","year":"1996","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.vetmic.2009.01.025","article-title":"Clostridium perfringens beta-toxin targets endothelial cells in necrotizing enteritis in piglets","volume":"137","author":"Miclard","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4396","DOI":"10.1128\/IAI.00547-08","article-title":"Effects of Clostridium perfringens beta-toxin on the rabbit small intestine and colon","volume":"76","author":"Vidal","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5200","DOI":"10.1128\/IAI.00534-06","article-title":"Dissecting the contributions of Clostridium perfringens type C toxins to lethality in the mouse intravenous injection model","volume":"74","author":"Fisher","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.2141\/jpsa.0160053","article-title":"Analysis of differentially expressed genes in necrotic enteritis-infected fayoumi chickens using RNA sequencing","volume":"54","author":"Hong","year":"2017","journal-title":"J. Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.vetimm.2015.06.008","article-title":"High-throughput sequencing reveals differing immune responses in the intestinal mucosa of two inbred lines afflicted with necrotic enteritis","volume":"166","author":"Truong","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1002\/2211-5463.12512","article-title":"Circular rna expression profile of spleen in a Clostridium perfringens type C-induced piglet model of necrotizing enteritis","volume":"8","author":"Yan","year":"2018","journal-title":"FEBS Open Bio"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0034-5288(18)31082-8","article-title":"Effect of creep feeding on the incidence, duration and severity of post-weaning diarrhoea in pigs","volume":"49","author":"Kelly","year":"1990","journal-title":"Res. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.5713\/ajas.2013.13067","article-title":"Swine leukocyte antigen-DQA gene variation and its association with piglet diarrhea in Large White, Landrace and Duroc","volume":"26","author":"Yang","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1093\/bioinformatics\/btp120","article-title":"Tophat: Discovering splice junctions with RNA-seq","volume":"25","author":"Trapnell","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nbt.1633","article-title":"Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs","volume":"28","author":"Guttman","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nprot.2012.016","article-title":"Differential gene and transcript expression analysis of RNA-seq experiments with Tophat and Cufflinks","volume":"7","author":"Trapnell","year":"2012","journal-title":"Nat. Protoc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/nbt.1621","article-title":"Transcript assembly and quantification by RNA-seq reveals unannotated transcripts and isoform switching during cell differentiation","volume":"28","author":"Trapnell","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"Huang","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta c(t)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2912418","DOI":"10.1155\/2016\/2912418","article-title":"Effect of high dietary tryptophan on intestinal morphology and tight junction protein of weaned pig","volume":"2016","author":"Tossou","year":"2016","journal-title":"BioMed Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Huang, G., Li, X., Lu, D., Liu, S., Suo, X., Li, Q., and Li, N. (2018). Lysozyme improves gut performance and protects against enterotoxigenic Escherichia coli infection in neonatal piglets. Vet. Res., 49.","DOI":"10.1186\/s13567-018-0511-4"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"901","DOI":"10.3382\/ps.2010-00907","article-title":"Transcriptional profiling of cecal gene expression in probiotic- and Salmonella-challenged neonatal chicks","volume":"90","author":"Higgins","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kim, D.K., Lillehoj, H.S., Jang, S.I., Lee, S.H., Hong, Y.H., and Cheng, H.H. (2014). Transcriptional profiles of host-pathogen responses to necrotic enteritis and differential regulation of immune genes in two inbreed chicken lines showing disparate disease susceptibility. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0114960"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Park, H.-E., Park, H.-T., Jung, Y.H., and Yoo, H.S. (2018). Gene expression profiles of immune-regulatory genes in whole blood of cattle with a subclinical infection of Mycobacterium avium subsp paratuberculosis. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0196502"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.17582\/journal.pjz\/2017.49.3.sc1","article-title":"RNA-seq reveals differentially expressed genes of pig vaccinated with modified live attenuated porcine epidemic diarrhea","volume":"49","author":"Park","year":"2017","journal-title":"Pak. J. Zool."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/s41467-017-02659-x","article-title":"Single-cell RNA-seq of rheumatoid arthritis synovial tissue using low-cost microfluidic instrumentation","volume":"9","author":"Stephenson","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1111\/1756-185X.13358","article-title":"RNA-seq analysis reveals different gene ontologies and pathways in rheumatoid arthritis and kashin-beck disease","volume":"21","author":"Yu","year":"2018","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Damiani, C., Maspero, D., Di Filippo, M., Colombo, R., Pescini, D., Graudenzi, A., Westerhoff, H.V., Alberghina, L., Vanoni, M., and Mauri, G. (2019). Integration of single-cell RNA-seq data into population models to characterize cancer metabolism. PLoS Comput. Biol., 15.","DOI":"10.1371\/journal.pcbi.1006733"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jgar.2018.10.008","article-title":"Identification and expression analysis of ceftriaxone resistance-related genes in neisseria gonorrhoeae integrating RNA-seq data and qRT-PCR validation","volume":"16","author":"Zhao","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.vetimm.2010.05.001","article-title":"Dietary zinc oxide affects the expression of genes associated with inflammation: Transcriptome analysis in piglets challenged with ETEC k88","volume":"137","author":"Sargeant","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1017\/S1751731116001178","article-title":"Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4","volume":"11","author":"Trevisi","year":"2017","journal-title":"Animal"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"7865","DOI":"10.1007\/s11033-014-3680-x","article-title":"Breed-specific transcriptome response of spleen from six to eight week old piglet after infection with Streptococcus suis type 2","volume":"41","author":"Gaur","year":"2014","journal-title":"Mol. Biol. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/cei.13236","article-title":"Colonization of preterm gnotobiotic piglets with probiotic lactobacillus rhamnosus gg and its interference with Salmonella typhimurium","volume":"195","author":"Splichalova","year":"2019","journal-title":"Clin. Exp. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.vaccine.2017.12.026","article-title":"Protective immunity of a multivalent vaccine candidate against piglet diarrhea caused by enterotoxigenic Escherichia coli (ETEC) in a pig model","volume":"36","author":"Zhang","year":"2018","journal-title":"Vaccine"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/j.1365-2958.2007.06007.x","article-title":"Beta toxin is essential for the intestinal virulence of Clostridium perfringens type C disease isolate CN3685 in a rabbit ileal loop model","volume":"67","author":"Sayeed","year":"2008","journal-title":"Mol. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s00360-015-0889-5","article-title":"A review of mixing and propulsion of chyme in the small intestine: Fresh insights from new methods","volume":"185","author":"Lentle","year":"2015","journal-title":"J. Comp. Physiol. B"},{"key":"ref_38","first-page":"186","article-title":"Influence of age at weaning and feeding regimes on the postnatal morphology of the porcine small intestine","volume":"23","author":"Hunigen","year":"2015","journal-title":"J. Swine Health Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.3382\/ps\/pey101","article-title":"Effect of dietary fructooligosaccharide supplementation on internal organs salmonella colonization, immune response, ileal morphology, and ileal immunohistochemistry in laying hens challenged with salmonella enteritidis","volume":"97","author":"Adhikari","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhou, C.L., Liu, Z.Z., Jiang, J.C., Yu, Y., and Zhang, Q. (2012). Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-330"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.bbadis.2011.09.019","article-title":"Alteration in intestine tight junction protein phosphorylation and apoptosis is associated with increase in IL-18 levels following alcohol intoxication and burn injury","volume":"1822","author":"Li","year":"2012","journal-title":"Biochim. Biophys. Acta-Mol. Basis Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/s41579-018-0036-x","article-title":"Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier","volume":"16","author":"Martens","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Lepine, A.F.P., de Wit, N., Oosterink, E., Wichers, H., Mes, J., and de Vos, P. (2018). Lactobacillus acidophilus attenuates Salmonella-induced stress of epithelial cells by modulating tight-junction genes and cytokine responses. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01439"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wang, J., Ji, H., Wang, S., Liu, H., Zhang, W., Zhang, D., and Wang, Y. (2018). Probiotic lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01953"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00535-018-1521-8","article-title":"Antimicrobial proteins: Intestinal guards to protect against liver disease","volume":"54","author":"Hendrikx","year":"2019","journal-title":"J. Gastroenterol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Bhat, A.A., Uppada, S., Achkar, I.W., Hashem, S., Yadav, S.K., Shanmugakonar, M., Al-Naemi, H.A., Haris, M., and Uddin, S. (2019). Tight junction proteins and signaling pathways in cancer and inflammation: A functional crosstalk. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01942"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9171905","DOI":"10.1155\/2018\/9171905","article-title":"Functions and signaling pathways of amino acids in intestinal inflammation","volume":"2018","author":"He","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nri3608","article-title":"Intestinal epithelial cells: Regulators of barrier function and immune homeostasis","volume":"14","author":"Peterson","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/ni.3153","article-title":"IL-6 as a keystone cytokine in health and disease","volume":"16","author":"Hunter","year":"2015","journal-title":"Nat. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1042\/CS20110340","article-title":"IL-6\/IL-6 receptor system and its role in physiological and pathological conditions","volume":"122","author":"Mihara","year":"2012","journal-title":"Clin. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1001\/jama.2011.1692","article-title":"Initiation of tumor necrosis factor-alpha antagonists and the risk of hospitalization for infection in patients with autoimmune diseases","volume":"306","author":"Grijalva","year":"2011","journal-title":"Jama"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1093\/rheumatology\/keq031","article-title":"Transmembrane TNF-alpha: Structure, function and interaction with anti-TNF agents","volume":"49","author":"Horiuchi","year":"2010","journal-title":"Rheumatology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/nri3322","article-title":"New concepts in the generation and functions of IgA","volume":"12","author":"Pabst","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/mi.2011.41","article-title":"Secretory IgA\u2019s complex roles in immunity and mucosal homeostasis in the gut","volume":"4","author":"Mantis","year":"2011","journal-title":"Mucosal Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1038\/icb.2016.123","article-title":"Interferon epsilon promotes hiv restriction at multiple steps of viral replication","volume":"95","author":"Eid","year":"2017","journal-title":"Immunol. Cell Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Sang, Y., Bergkamp, J., and Blecha, F. (2014). Molecular evolution of the porcine type I interferon family: Subtype-specific expression and antiviral activity. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112378"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Hielpos, M.S., Ferrero, M.C., Fernandez, A.G., Bonetto, J., Giambartolomei, G.H., Fossati, C.A., and Baldi, P.C. (2015). CCL20 and beta-defensin 2 production by human lung epithelial cells and macrophages in response to brucella abortus infection. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0140408"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1093\/infdis\/jiv206","article-title":"CCL20 displays antimicrobial activity against cryptosporidium parvum, but its expression is reduced during infection in the intestine of neonatal mice","volume":"212","author":"Guesdon","year":"2015","journal-title":"J. Infect. Dis."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/5\/269\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:54:30Z","timestamp":1760187270000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/5\/269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,23]]},"references-count":58,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5]]}},"alternative-id":["ani9050269"],"URL":"https:\/\/doi.org\/10.3390\/ani9050269","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2019,5,23]]}}}