{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T21:21:16Z","timestamp":1775078476449,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T00:00:00Z","timestamp":1569196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660662"],"award-info":[{"award-number":["31660662"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of Qinghai Province","award":["2016-ZJ-935Q"],"award-info":[{"award-number":["2016-ZJ-935Q"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>The jejunum is the primary organ for digestion and nutrient absorption in mammals. The development of the jejunum in suckling piglets directly affects their growth performance post-weaning. The jejunum microbiome plays an important role in proliferation, metabolism, apoptosis, immune, and homeostasis of the epithelial cells within the organ. The composition and diversity of the gut microbiome is susceptible to the protein composition of the diet. Therefore, the effects of maternal low-protein diets on piglets\u2019 intestinal microbial structure and function have become a hot topic of study. Herein, a maternal low-protein diet was formulated to explore the effects on jejunum microbiome composition and metabolic profiles in Bamei suckling piglets. Using 16S ribosomal RNA (16S rRNA) sequencing in conjunction with bioinformatics analysis, 21 phyla and 297 genera were identified within the gut microflora. The top 10 phyla and 10 genera are within the gut bacteria. Next, KEGG analysis showed that the low-protein diet significantly increased the gut microbial composition, transport and catabolism, immune system, global and overview maps, amino acid metabolism, metabolism of cofactors and vitamins, endocrine system, biosynthesis of other secondary metabolites, signal transduction, environmental adaptation, and cell motility. Taken together, low-protein diets do not appear to affect the reproductive performance of Bamei sows but improved the gut microbiome of the suckling piglets as well as reduced the probability of diarrhea. The data presented here provide new insights on the dietary protein requirements to support the Huzhu Bamei pig industry.<\/jats:p>","DOI":"10.3390\/ani9100713","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T11:02:00Z","timestamp":1569236520000},"page":"713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of Maternal Low-Protein Diet on Microbiota Structure and Function in the Jejunum of Huzhu Bamei Suckling Piglets"],"prefix":"10.3390","volume":"9","author":[{"given":"Jipeng","family":"Jin","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlei","family":"Jia","sequence":"additional","affiliation":[{"name":"Key of Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China"},{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zan","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weibo","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cunming","family":"Ma","sequence":"additional","affiliation":[{"name":"Qinghai Province Huzhu County Bamei Pig Seed Breeding Farm, Huzhu, Qinghai 810500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fafang","family":"Xu","sequence":"additional","affiliation":[{"name":"Qinghai Province Huzhu County Bamei Pig Seed Breeding Farm, Huzhu, Qinghai 810500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoujun","family":"Zhan","sequence":"additional","affiliation":[{"name":"Qinghai Province Huzhu County Bamei Pig Seed Breeding Farm, Huzhu, Qinghai 810500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limin","family":"Ma","sequence":"additional","affiliation":[{"name":"Qinghai Province Huzhu County Bamei Pig Seed Breeding Farm, Huzhu, Qinghai 810500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guihua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Qinghai Province Huzhu County Bamei Pig Seed Breeding Farm, Huzhu, Qinghai 810500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.it.2014.07.010","article-title":"Modulation of immune development and function by intestinal microbiota","volume":"35","author":"Kabat","year":"2014","journal-title":"Trends Immunol."},{"key":"ref_2","first-page":"201","article-title":"Effects of gut microbes on nutrient absorption and energy regulation","volume":"27","author":"Rosa","year":"2015","journal-title":"Nutr. Clin. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1038\/s41598-018-20967-0","article-title":"Diversity and metabolic potential of the microbiota associated with a soil arthropod","volume":"8","author":"Bahrndorff","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3103\/S1068367418010135","article-title":"Effects of Low-Protein Diets with Different Levels of Essential Amino Acids and Metabolizable Energy on Productivity and Metabolic Processes in Pigs","volume":"44","author":"Niyazov","year":"2018","journal-title":"Russ. Agric. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/MCO.0000000000000478","article-title":"Gut nutrient sensing and microbiota function in the control of energy homeostasis","volume":"21","author":"Mithieux","year":"2018","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"R105","DOI":"10.1530\/JOE-17-0542","article-title":"Gut-brain signaling in energy homeostasis: The unexpected role of microbiota-derived succinate","volume":"236","author":"Vadder","year":"2018","journal-title":"J. Endocrinol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"230","DOI":"10.4049\/jimmunol.1701216","article-title":"Innate Recognition of the Microbiota by TLR1 Promotes Epithelial Homeostasis and Prevents Chronic Inflammation","volume":"201","author":"Kamdar","year":"2018","journal-title":"J. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s40104-018-0276-7","article-title":"Advances in low-protein diets for swine","volume":"9","author":"Wang","year":"2018","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1017\/S1751731113000062","article-title":"Impact of dietary protein on microbiota composition and activity in the gastrointestinal tract of piglets in relation to gut health: A review","volume":"7","author":"Rist","year":"2013","journal-title":"Animal"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3389\/fcimb.2018.00246","article-title":"Moderate Dietary Protein Restriction Optimized Gut Microbiota and Mucosal Barrier in Growing Pig Model","volume":"8","author":"Chen","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1093\/jas\/sky047","article-title":"Whole-Body Nitrogen Utilization and Tissue Protein and Casein Synthesis in Lactating Primiparous Sows Fed Low- and High- Protein Diets","volume":"96","author":"Huber","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1439-0396.2007.00804.x","article-title":"Comparison of total tract digestibility, development of visceral organs and digestive tract of Mong cai and Yorkshire x Landrace piglets fed diets with different fibre sources","volume":"93","author":"Len","year":"2010","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1017\/S1751731116000999","article-title":"Effects of low-protein diets supplemented with indispensable amino acids on growth performance, intestinal morphology and immunological parameters in 13 to 35 kg pigs","volume":"10","author":"Peng","year":"2016","journal-title":"Animal"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"574","DOI":"10.3390\/nu3050574","article-title":"Dietary proteins as determinants of metabolic and physiologic functions of the gastrointestinal tract","volume":"3","author":"Alireza","year":"2011","journal-title":"Nutrients"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"637","DOI":"10.2527\/jas.2013-6514","article-title":"The use of free amino acids allows formulating very low crude protein diets for piglets","volume":"92","author":"Gloaguen","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1631\/jzus.B1600229","article-title":"Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets","volume":"17","author":"Tian","year":"2016","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Tanja","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1038\/ismej.2011.186","article-title":"Comparison of Illumina paired-end and single-direction sequencing for microbial 16S rRNA gene amplicon surveys","volume":"6","author":"Werner","year":"2012","journal-title":"ISME J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3389\/fnagi.2018.00156","article-title":"Detection of Microbial 16S rRNA Gene in the Blood of Patients with Parkinson\u2019s Disease","volume":"10","author":"Qian","year":"2018","journal-title":"Front. Aging Neurosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1128\/AEM.67.6.2578-2585.2001","article-title":"Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis","volume":"67","author":"Walter","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","first-page":"117","article-title":"Effects of feeding low protein diet with and without leucine supplementation on protein status of lactating females and their pups","volume":"35","author":"Chugh","year":"1991","journal-title":"Indian J. Physiol. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1677\/joe.0.1730131","article-title":"The effect of maternal undernutrition on ovine fetal growth","volume":"173","author":"Osgerby","year":"2002","journal-title":"J. Endocrinol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40781-017-0153-5","article-title":"Effects of amino acid composition in pig diet on odorous compounds and microbial characteristics of swine excreta","volume":"59","author":"Recharla","year":"2017","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1079\/NRR200242","article-title":"Nutritional influences on some major enteric bacterial diseases of pig","volume":"15","author":"Pluske","year":"2002","journal-title":"Nutr. Res. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1038\/ismej.2014.12","article-title":"Bacteria, phages and pigs: The effects of in-feed antibiotics on the microbiome at different gut locations","volume":"8","author":"Looft","year":"2014","journal-title":"ISME J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/sj.embor.7400731","article-title":"The gut flora as a forgotten organ","volume":"7","author":"Shanahan","year":"2006","journal-title":"EMBO Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.tifs.2013.08.007","article-title":"Interactions between gut microbiota, food and the obese host","volume":"34","author":"Requena","year":"2013","journal-title":"Trends Food Sci. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/nrmicro2876","article-title":"The interplay between the intestinal microbiota and the brain","volume":"10","author":"Collins","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.3945\/jn.108.103267","article-title":"Dietary and endogenous amino acids are the main contributors to microbial protein in the upper gut of normally nourished pigs","volume":"139","author":"Zhu","year":"2009","journal-title":"J. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/j.jaci.2017.02.011","article-title":"Correlation between early-life regulation of the immune system by microbiota and allergy development","volume":"139","author":"Gensollen","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"34","DOI":"10.3389\/fnut.2017.00034","article-title":"The Role of Supplemental Complex Dietary Carbohydrates and Gut Microbiota in Promoting Cardiometabolic and Immunological Health in Obesity: Lessons from Healthy Non-Obese Individuals","volume":"4","author":"Vinke","year":"2017","journal-title":"Front. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1254766","DOI":"10.1126\/science.1254766","article-title":"HUMAN MICROBIOTA. Small molecules from the human microbiota","volume":"349","author":"Donia","year":"2015","journal-title":"Science"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5996","DOI":"10.3168\/jds.2016-12239","article-title":"Understanding the gut microbiome of dairy calves: Opportunities to improve early-life gut health","volume":"100","author":"Malmuthuge","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_36","first-page":"1","article-title":"Maternal obesity is associated with gut microbial metabolic potential in offspring during infancy","volume":"74","author":"Ruiz","year":"2018","journal-title":"J. Physiol. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"16024","DOI":"10.1038\/nmicrobiol.2016.24","article-title":"Developmental dynamics of the preterm infant gut microbiota and antibiotic resistome","volume":"1","author":"Gibson","year":"2016","journal-title":"Nat. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12902","DOI":"10.1073\/pnas.1717163115","article-title":"Microbiome as a tool and a target in the effort to address antimicrobial resistance","volume":"115","author":"Relman","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s12020-018-1719-9","article-title":"Maternal low-protein diet during lactation combined with early overfeeding impair male offspring\u2019s long-term glucose homeostasis","volume":"63","author":"Almeida","year":"2019","journal-title":"Endocrine"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3389\/fimmu.2018.00005","article-title":"Nutrients Mediate Intestinal Bacteria\u2013Mucosal Immune Crosstalk","volume":"9","author":"Ma","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"k2179","DOI":"10.1136\/bmj.k2179","article-title":"Role of the gut microbiota in nutrition and health","volume":"361","author":"Valdes","year":"2018","journal-title":"BMJ Clin. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1002\/jobm.201100500","article-title":"Antibiotics production by an actinomycete isolated from the termite gut","volume":"52","author":"Toru","year":"2012","journal-title":"J. Basic Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.3389\/fmicb.2017.01215","article-title":"Outer Membrane Proteome of Veillonella parvula: A Diderm Firmicute of the Human Microbiome","volume":"8","author":"Poppleton","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s40168-017-0242-1","article-title":"New evidences on the altered gut microbiota in autism spectrum disorders","volume":"5","author":"Strati","year":"2017","journal-title":"Microbiome"},{"key":"ref_45","first-page":"307ra152","article-title":"Early infancy microbial and metabolic alterations affect risk of childhood asthma","volume":"7","author":"Stiemsma","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e20160218","DOI":"10.1542\/peds.2016-0218","article-title":"The Fecal Microbiota Profile and Bronchiolitis in Infants","volume":"138","author":"Hasegawa","year":"2016","journal-title":"Pediatrics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"43412","DOI":"10.1038\/srep43412","article-title":"Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model","volume":"7","author":"Fan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrgastro.2016.135","article-title":"Gut microbiota: Changes in gut microbes and host metabolism: Squaring the circle?","volume":"13","author":"Cani","year":"2016","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1136\/gutjnl-2015-309990","article-title":"The gut microbiota and host health: A new clinical frontier","volume":"65","author":"Marchesi","year":"2016","journal-title":"Gut"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1021\/acs.jmedchem.7b00244","article-title":"Microbiota-host Transgenomic Metabolism, Bioactive Molecules from the Inside","volume":"61","author":"Turroni","year":"2017","journal-title":"J. Med. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.2527\/jas.2007-0105","article-title":"Effect of dietary protein content on ileal amino acid digestibility, growth performance, and formation of microbial metabolites in ileal and cecal digesta of early-weaned pigs","volume":"85","author":"Htoo","year":"2007","journal-title":"J. Anim. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"592","DOI":"10.2174\/1389203716666150630135720","article-title":"Interaction between Microbes and Host Intestinal Health: Modulation by Dietary Nutrients and Gut-Brain-Endocrine-Immune Axis","volume":"16","author":"Chen","year":"2015","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.2527\/jas.2008-1310","article-title":"Effect of dietary protein level on growth performance, indicators of enteric health, and gastrointestinal microbial ecology of weaned pigs induced with postweaning colibacillosis","volume":"87","author":"Opapeju","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2566","DOI":"10.1128\/JB.01359-06","article-title":"Metabolism of linoleic acid by human gut bacteria: Different routes for biosynthesis of conjugated linoleic acid","volume":"189","author":"Estelle","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"646","DOI":"10.2174\/1389203716666150630133657","article-title":"Metabolites of Dietary Protein and Peptides by Intestinal Microbes and their Impacts on Gut","volume":"16","author":"Fan","year":"2015","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1016\/j.febslet.2014.04.007","article-title":"Microbial view of central nervous system autoimmunity","volume":"588","author":"Berer","year":"2014","journal-title":"FEBS Lett."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0092-8674(78)90306-9","article-title":"The effect of sodium butyrate on histone modification","volume":"14","author":"Sealy","year":"1978","journal-title":"Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1210\/me.2014-1108","article-title":"Minireview: Gut microbiota: The neglected endocrine organ","volume":"28","author":"Gerard","year":"2014","journal-title":"Mol. Endocrinol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/10\/713\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:23:09Z","timestamp":1760188989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/10\/713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,23]]},"references-count":58,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["ani9100713"],"URL":"https:\/\/doi.org\/10.3390\/ani9100713","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,23]]}}}