{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T04:57:33Z","timestamp":1778648253316,"version":"3.51.4"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Saccharomyces cerevisiae and Aspergillus oryzae are both ancient probiotic species traditionally used as microbes for brewing beer and soy sauce, respectively. This study investigated the effect of adding these two probiotics with phytase fermentation products to the broilers diet. Fermented products possess protease and cellulase, and the activities were 777.1 and 189.5 U\/g dry matter (DM) on S. cerevisiae fermented products (SCFP) and 190 and 213.4 U\/g DM on A. oryzae fermented products (AOFP), respectively. Liposaccharides stimulated PBMCs to produce nitric oxide to 120 \u03bcmol. Both SCFP and AOFP reduced lipopolysaccharides stimulated peripheral blood mononuclear cells (PBMCs) nitric oxide release to 40 and 60 \u03bcmol, respectively. Nevertheless, in an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, SCFP and AOFP also increased the survival rate of lipopolysaccharides stimulated PBMCs by almost two-fold compared to the negative control. A total of 240 broilers were divided into four groups as Control, SCFP 0.1% (SCFP), SCFP 0.05% + AOFP 0.05% (SAFP), and AOFP 0.1% (AOFP) groups, respectively. Each group had 20 broilers, and three replicate pens. The results showed that the addition of SCFP, SAFP, and AOFP groups did not affect the growth performances, but increased the jejunum value of villus height and villus: crypt ratio on SAFP and AOFP groups compared to the control and SCFP groups. Furthermore, adding SCFP, SAFP, and AOFP significantly reduced the number of Clostridium perfringens in ileum chyme. SCFP, SAFP, and AOFP significantly reduced the amount of interleukin-1\u03b2, inducible nitric oxide synthases, interferon-\u03b3, and nuclear factor kappa B mRNA expression in PBMCs, especially in the AOFP group. In summary, all the SCFP, SAFP, and AOFP groups can be suggested as a functional feed additive since they enhanced villus: crypt ratio and decreased inflammation-related mRNA expression, especially for AOFP group in broilers.<\/jats:p>","DOI":"10.3390\/ani9121051","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T10:50:45Z","timestamp":1575283845000},"page":"1051","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Evaluation of the Combined Use of Saccharomyces Cerevisiae and Aspergillus Oryzae with Phytase Fermentation Products on Growth, Inflammatory, and Intestinal Morphology in Broilers"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5282-5878","authenticated-orcid":false,"given":"Wen Yang.","family":"Chuang","sequence":"first","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei Chih.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Chen.","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung Ming.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shen Chang.","family":"Chang","sequence":"additional","affiliation":[{"name":"Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Pingtung 912, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu-Tai","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan"},{"name":"The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.jcs.2007.09.008","article-title":"Dry processes to develop wheat fractions and products with enhanced nutritional quality","volume":"46","author":"Hemery","year":"2007","journal-title":"J. Cereal Sci."},{"key":"ref_2","first-page":"197","article-title":"Wheat bran (Triticum aestivum): Composition, functionality and incorporation in unleavened bread","volume":"25","author":"Shenoy","year":"2007","journal-title":"J. Food Qual."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0377-8401(94)90156-2","article-title":"Total phosphorus, phytate phosphorus and phytase activity in plant feedstuffs","volume":"47","author":"Eeckhout","year":"1994","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_4","first-page":"82","article-title":"Effect of a specific combination of mannan-oligosaccharides and \u03b2-glucans extracted from yeast cell wall on the health status and growth performance of ochratoxicated broiler chickens","volume":"7","author":"Awaad","year":"2011","journal-title":"J. Am. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2141\/jpsa.0160069","article-title":"Administration of Bacillus amyloliquefaciens and Saccharomyces cerevisiae as direct-fed microbials improves intestinal microflora and morphology in broiler chickens","volume":"54","author":"Teng","year":"2017","journal-title":"J. Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1023\/A:1008868905587","article-title":"Production of extracellular lipases by saccharomyces cerevisiae","volume":"14","author":"Shirazi","year":"1998","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2141\/jpsa.0130022","article-title":"An evaluation of cassava pulp feedstuff fermented with A. oryzae, on growth performance, nutrient digestibility and carcass quality of broilers","volume":"51","author":"Khempaka","year":"2013","journal-title":"J. Poult. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1080\/00071668.2017.1280772","article-title":"Antioxidant molecular targets of wheat bran fermented by white rot fungi and its potential modulation of antioxidative status in broiler chickens","volume":"58","author":"Wang","year":"2017","journal-title":"Br. Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4145","DOI":"10.3748\/wjg.v16.i33.4145","article-title":"Effect of probiotics on pro-inflammatory cytokines and NF-\u03baB activation in ulcerative colitis","volume":"16","author":"Hegazy","year":"2010","journal-title":"World J. Gastroenterol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1080\/07315724.2015.1080105","article-title":"Anti-inflammatory Diets","volume":"34","author":"Sears","year":"2015","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"226.3","DOI":"10.4049\/jimmunol.198.Supp.226.3","article-title":"Relievable effect of dietary Allium hookeri on LPS induced intestinal inflammation response in young broiler chickens","volume":"198","author":"Lee","year":"2017","journal-title":"J. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.anifeedsci.2017.01.008","article-title":"A systematic view on the effect of phytase on ileal amino acid digestibility in broilers","volume":"225","author":"Cowieson","year":"2017","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.anifeedsci.2018.07.005","article-title":"The effects of microbisal phytase and dietary calcium and phosphorus levels on the productive performance and bone mineralization of broilers","volume":"243","author":"Hamdi","year":"2018","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1365-2672.1983.tb01321.x","article-title":"The adherence of lactic acid bacteria to the columnar epithelial cells of pigs and calves","volume":"55","author":"Annika","year":"1983","journal-title":"J. Appl. Bacteriol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1021\/ac60147a030","article-title":"Use of dinitrosalicylic acid reagent for determination of reducing sugar","volume":"31","author":"Miller","year":"1959","journal-title":"Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1016\/j.procbio.2004.12.001","article-title":"Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation","volume":"40","author":"Sandhya","year":"2005","journal-title":"Process. Biochem."},{"key":"ref_17","first-page":"7122","article-title":"Determination methods of cellulase activity","volume":"10","author":"Tian","year":"2011","journal-title":"Afr. J. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1093\/ps\/85.11.1907","article-title":"Cytokine expression in chicken peripheral blood mononuclear cells after in vitro exposure to Salmonella enterica serovar enteritidis","volume":"85","author":"Kaiser","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_19","unstructured":"AOAC (2012). Official Methods of Analysis of AOAC International, AOAC International. [19th ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ecoenv.2018.08.029","article-title":"Hydrogen sulfide inhalation-induced immune damage is involved in oxidative stress, inflammation, apoptosis and the Th1\/Th2 imbalance in broiler bursa of Fabricius","volume":"164","author":"Hu","year":"2018","journal-title":"Ecotox Environ. Safe."},{"key":"ref_21","first-page":"100367","article-title":"Effect of different cereal-based diets supplemented with multi-enzyme blend on growth performance villus structure and gene expression (SGLT1, GLUT2, PepT1 and MUC2) in the small intestine of broiler chickens","volume":"15","author":"Kalantar","year":"2019","journal-title":"Gene Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.5713\/ajas.2012.12445","article-title":"Effect of high phytase inclusion rates on performance of broilers fed diets not severely limited in available phosphorus","volume":"26","author":"Santos","year":"2013","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.3382\/ps\/pev243","article-title":"Evaluation of yeast dietary supplementation in broilers challenged or not with Salmonella on growth performance, cecal microbiota composition and Salmonella in ceca, cloacae and carcass skin","volume":"94","author":"Mountzouris","year":"2015","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1093\/ps\/86.6.1149","article-title":"Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers","volume":"86","author":"Feng","year":"2007","journal-title":"Poult. Sci."},{"key":"ref_25","first-page":"150","article-title":"Uric acid and cardiovascular disease","volume":"484","author":"Ndrepepa","year":"2018","journal-title":"Eur. Caediol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1089\/jmf.2004.7.430","article-title":"Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals","volume":"7","author":"Hong","year":"2004","journal-title":"J. Med. Food."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.3382\/ps.2007-00418","article-title":"Effects of yeast culture in broiler diets on performance and immunomodulatory functions","volume":"87","author":"Gao","year":"2008","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1096\/fj.201600891R","article-title":"Benzopyrimido-pyrrolo-oxazine-dione CFTR inhibitor (R)-BPO-27 for anti-secretory therapy of diarrheas caused by bacterial enterotoxins","volume":"31","author":"Cil","year":"2016","journal-title":"FASEB J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2018.10.022","article-title":"Clostridium perfringens-mediated necrotic enteritis is not influenced by the pre-existing microbiota but is promoted by large changes in the post-challenge microbiota","volume":"227","author":"Lacey","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"309","DOI":"10.5713\/ajas.18.0538","article-title":"Potential crosstalk of oxidative stress and immune response in poultry through phytochemicals\u2014A review","volume":"32","author":"Lee","year":"2019","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nrrheum.2010.4","article-title":"IL-1 pathways in inflammation and human diseases","volume":"6","author":"Gabay","year":"2010","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Municio, C., Alvarez, Y., Montero, O., Hugo, E., Rodr\u0131\u2019guez, M., Domingo, E., Alonso, S., Ferna\u2019ndez, N., and Crespo, M.S. (2013). The response of human macrophages to \u00df-glucans depends on the inflammatory milieu. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062016"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"617","DOI":"10.5713\/ajas.17.0657","article-title":"Immunomodulatory effects of phytogenics in chickens and pigs\u2014A review","volume":"31","author":"Huang","year":"2017","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"763","DOI":"10.2174\/1566524043359908","article-title":"The role of iNOS in chronic inflammatory processes in vivo: Is it damage-promoting, protective, or active at all?","volume":"4","author":"Suschek","year":"2004","journal-title":"Curr. Mol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.cellsig.2010.10.017","article-title":"Role of nitric oxide produced by iNOS through NF-\u03baB pathway in migration of cerebellar granule neurons induced by lipopolysaccharide","volume":"23","author":"Silbergeld","year":"2011","journal-title":"Cell Signal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/nri3581","article-title":"Regulation of type I interferon responses","volume":"14","author":"Ivashkiv","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1136\/jcp.2004.023507","article-title":"Interferon gamma accelerates NF-\u03baB activation of biliary epithelial cells induced by Toll-like receptor and ligand interaction","volume":"59","author":"Harada","year":"2006","journal-title":"J. Clin. Pathol."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/12\/1051\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:39:06Z","timestamp":1760189946000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/12\/1051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,1]]},"references-count":37,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["ani9121051"],"URL":"https:\/\/doi.org\/10.3390\/ani9121051","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,1]]}}}