{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:43:13Z","timestamp":1790995393857,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>To evaluate the effects of dietary Se deficiency and excess on the mRNA levels of selenoproteins in pig spleen tissues, 20 healthy uncastrated boars (Duroc \u00d7 Landrace \u00d7 Yorkshire, 10 \u00b1 0.72 kg) were randomly divided into four groups (5 pigs per group). The pigs were fed a Se deficient corn-soybean basal feed (Se content &lt;0.03 mg\/kg) or basal feed with added sodium selenite at 0.3, 1.0, or 3.0 mg Se\/kg diet, respectively. The experiment lasted 16 weeks. The spleen tissue was collected to examine the mRNA expression levels of 24 selenoprotein genes at the end of the study. Compared with pigs in other groups, those fed with the 1.0 mg Se\/kg diet had higher mRNA levels of glutathione peroxidase 1 (Gpx1), glutathione peroxidase 2 (Gpx2), deiodinase type II (Dio2), thioredoxin reductase 3 (Txnrd3), selenoprotein H (Selh), selenoprotein N, 1 (Sepn1), selenoprotein P1 (Sepp1), and selenoprotein V (Selv) in the spleen (p &lt; 0.05). Dietary Se deficiency resulted in lower mRNA levels of Gpx1, Gpx2, glutathione peroxidase 3 (Gpx3), Dio2, thioredoxin reductase 2 (Txnrd2), Txnrd3, Selh, selenoprotein I (Seli), selenoprotein K (Selk), selenoprotein M (Selm), Sepn1, Sepp1, and Selv in the spleen than the other three groups. Dietary Se levels did not affect the mRNA levels of glutathione peroxidase 4 (Gpx4), deiodinase type I (Dio1), deiodinase type III (Dio3), selenophosphate synthetase 2 (Sephs2), thioredoxin reductase 1 (Txnrd1), selenoprotein O (Selo), selenoprotein S (Sels), selenoprotein W (Selw), selenoprotein X (Selx), and selenoprotein 15 (Sel15) in the spleen (p &gt; 0.05). Dietary Se levels can affect the transcription levels of 14 selenoprotein genes in the spleen of pigs.<\/jats:p>","DOI":"10.3390\/ani9121122","type":"journal-article","created":{"date-parts":[[2019,12,12]],"date-time":"2019-12-12T03:20:16Z","timestamp":1576120816000},"page":"1122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Effects of Dietary Selenium Deficiency or Excess on Selenoprotein Gene Expression in the Spleen Tissue of Pigs"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1826-363X","authenticated-orcid":false,"given":"Zhuang","family":"Lu","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengzu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Teng","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoming","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshan","family":"Shan","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Gen","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1016\/j.jinorgbio.2006.05.018","article-title":"Selenoprotein W during development and oxidative stress","volume":"100","author":"Loflin","year":"2006","journal-title":"J. Inorg. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1007\/s10534-010-9334-0","article-title":"Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by selenium","volume":"23","author":"Li","year":"2010","journal-title":"Biometals"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/biof.5520140120","article-title":"An analysis of cancer prevention by selenium","volume":"14","author":"Combs","year":"2001","journal-title":"Biofactors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/mus.10304","article-title":"Skeletal muscle disorders associated with selenium deficiency in humans","volume":"27","author":"Chariot","year":"2003","journal-title":"Muscle Nerve."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.livsci.2008.12.005","article-title":"Levels of mRNA for three selenoproteins in skeletal muscle of fetal and newborn pigs","volume":"124","author":"Terry","year":"2009","journal-title":"Livest. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0140-6736(00)02490-9","article-title":"The importance of selenium to human health","volume":"356","author":"Rayman","year":"2000","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1093\/jn\/134.4.707","article-title":"The neurobiology of selenium: Lessons from transgenic mice","volume":"134","author":"Schweizer","year":"2004","journal-title":"J. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"29798","DOI":"10.1074\/jbc.M100422200","article-title":"Selenium metabolism in Drosophila: Selenoproteins, selenoprotein mRNA expression, fertility, and mortality","volume":"276","author":"Kryukov","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.nut.2004.05.028","article-title":"Effect of selenium-induced oxidative stress on the cell kinetics in testis and reproductive ability of male mice","volume":"21","author":"Kaur","year":"2005","journal-title":"Nutrition"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/S0167-5699(98)01294-8","article-title":"Selenium: An essential element for immune function","volume":"19","author":"McKenzie","year":"1998","journal-title":"Immunol. Today"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1002\/mnfr.200700330","article-title":"The influence of selenium on immune responses","volume":"52","author":"Hoffmann","year":"2008","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.etp.2011.03.001","article-title":"Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats","volume":"64","author":"Oda","year":"2012","journal-title":"Exp. Toxicol. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0140-6736(83)90116-2","article-title":"Prediagnostic serum selenium and risk of cancer","volume":"322","author":"Willett","year":"1983","journal-title":"Lancet"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"385","DOI":"10.7589\/0090-3558-33.3.385","article-title":"Diagnostic criteria for selenium toxicosis in aquatic birds: Histologic lesions","volume":"33","author":"Green","year":"1997","journal-title":"J. Wildl. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s12011-009-8345-6","article-title":"The decrease of relative weight, lesions, and apoptosis of bursa of Fabricius induced by excess dietary selenium in chickens","volume":"131","author":"Peng","year":"2009","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.fct.2006.12.021","article-title":"Non-optimal levels of dietary selenomethionine alter splenocyte response and modify oxidative stress markers in female mice","volume":"45","author":"Vega","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jtemb.2007.10.002","article-title":"Effect of selenium status and supplementary selenoi-chemical sources on mouse T-cell mitogenesis","volume":"22","author":"Ueno","year":"2008","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1007\/s12011-010-8679-0","article-title":"Histological lesion of spleen and inhibition of splenocyte proliferation in broilers fed on diets excess in selenium","volume":"140","author":"Peng","year":"2011","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1146\/annurev.nutr.21.1.453","article-title":"Mammalian seleniumcontaining proteins","volume":"21","author":"Behne","year":"2001","journal-title":"Annu. Rev. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1111\/j.1749-6632.2000.tb06203.x","article-title":"Selenium biochemistry. Mammalian selenoenzymes","volume":"899","author":"Stadtman","year":"2000","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.3945\/jn.109.104901","article-title":"Selenoprotein gene expression in thyroid and pituitary of young pigs is not affected by dietary selenium deficiency or excess","volume":"139","author":"Zhou","year":"2009","journal-title":"J. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.3945\/jn.111.145722","article-title":"The selenium deficiency disease exudative diathesis in chicks is associated with downregulation of seven common selenoprotein genes in liver and muscle","volume":"141","author":"Huang","year":"2011","journal-title":"J. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.3945\/jn.112.159020","article-title":"Prolonged dietary selenium deficiency or excess does not globally affect selenoprotein gene expression and\/or protein production in various tissues of pigs","volume":"142","author":"Liu","year":"2012","journal-title":"J. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20181","DOI":"10.1074\/jbc.M802559200","article-title":"Selenoproteins mediate T cell immunity through an antioxidant mechanism","volume":"283","author":"Shrimali","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1146\/annurev.nu.07.070187.002045","article-title":"The pig as a model for human nutrition","volume":"7","author":"Miller","year":"1987","journal-title":"Annu. Rev. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1079\/BJN19940108","article-title":"Comparison of the ileal and faecal digestibility of dietary amino acids in adult humans and evaluation of the pig as a model animal for digestion studies in man","volume":"71","author":"Rowan","year":"1994","journal-title":"Br. J. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1046\/j.1524-475x.2001.00066.x","article-title":"The pig as a model for human wound healing","volume":"9","author":"Sullivan","year":"2001","journal-title":"Wound Repair Regen."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.1210\/en.2004-0356","article-title":"Characteristics and thyroid state-dependent regulation of iodothyronine deiodinases in pigs","volume":"145","author":"Wassen","year":"2004","journal-title":"Endocrinology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394Ct Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"425","DOI":"10.3181\/00379727-182-42361","article-title":"Effect of selenium and vitamin E dietary deficiencies on chick lymphoid organ development","volume":"182","author":"Marsh","year":"1986","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1007\/s00018-009-0032-4","article-title":"The human selenoproteome: Recent insights into functions and regulation","volume":"66","author":"Reeves","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/jn\/128.1.130","article-title":"Dietary selenium supplementation is required to support full expression of three selenium-dependent glutathione peroxidases in various tissues of weanling pigs","volume":"128","author":"Lei","year":"1998","journal-title":"J. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.jnutbio.2008.06.009","article-title":"The gastrointestinal microbiota affects the selenium status and selenoprotein expression in mice","volume":"20","author":"Hrdina","year":"2009","journal-title":"J. Nutr. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/2049-1891-5-46","article-title":"Effects of dietary Selenomethionine supplementation on growth performance, antioxidant status, plasma selenium concentration, and immune function in weaning pigs","volume":"5","author":"Cao","year":"2014","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_35","first-page":"26","article-title":"Cracking the code for thyroid hormone signaling","volume":"124","author":"Bianco","year":"2012","journal-title":"Trans. Am. Clin. Climatol. Assoc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s12011-014-0083-8","article-title":"Selenium deficiency inhibits the conversion of thyroidal thyroxine (T4) to triiodothyronine (T3) in chicken thyroids","volume":"161","author":"Lin","year":"2014","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3945\/an.110.000240","article-title":"Selenium regulation of the selenoprotein and nonselenoprotein transcriptomes in rodents","volume":"2","author":"Sunde","year":"2011","journal-title":"Adv. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1139\/y91-117","article-title":"Impairment of iodothyronine 5\u2032-deiodinase activity in brown adipose tissue and its acute stimulation by cold in selenium deficiency","volume":"69","author":"Arthur","year":"1991","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1016\/j.jinorgbio.2011.05.022","article-title":"Dietary selenium influences pancreatic tissue levels of selenoprotein W in chickens","volume":"105","author":"Wang","year":"2011","journal-title":"J. Inorg. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/s13258-010-0127-1","article-title":"Molecular cloning, chromosomal localization and expression profiling of porcine selenoprotein M gene","volume":"33","author":"Zhou","year":"2011","journal-title":"Genes Genom."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"24522","DOI":"10.1074\/jbc.274.35.24522","article-title":"Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases","volume":"274","author":"Sun","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1074\/jbc.R800045200","article-title":"Selenoproteins","volume":"284","author":"Lu","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s12011-014-0005-9","article-title":"Effects of dietary selenium deficiency on mrna levels of twenty-one selenoprotein genes in the liver of layer chicken","volume":"159","author":"Liu","year":"2014","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"11960","DOI":"10.1074\/jbc.M701605200","article-title":"Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern","volume":"282","author":"Novoselov","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.abb.2010.08.001","article-title":"SelK is a novel ER stress-regulated protein and protects HepG2 cells from ER stress agent-induced apoptosis","volume":"502","author":"Du","year":"2010","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"16891","DOI":"10.1074\/jbc.M109.006288","article-title":"Selenoprotein S\/SEPS1 modifies endoplasmic reticulum stress in Z variant alpha1-antitrypsin deficiency","volume":"284","author":"Kelly","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1007\/s12011-011-9062-5","article-title":"Effects of dietary selenium on selenoprotein W gene expression in the chicken immune organs","volume":"144","author":"Yu","year":"2011","journal-title":"Biol. Trace Elem. Res."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/12\/1122\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:41:32Z","timestamp":1760190092000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/9\/12\/1122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,11]]},"references-count":47,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["ani9121122"],"URL":"https:\/\/doi.org\/10.3390\/ani9121122","relation":{},"ISSN":["2076-2615"],"issn-type":[{"value":"2076-2615","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,11]]}}}