{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:03:14Z","timestamp":1767859394148,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of Pharmacy and Biochemistry, Genos Ltd.","award":["KK.01.1.1.01.0010"],"award-info":[{"award-number":["KK.01.1.1.01.0010"]}]},{"name":"Faculty of Pharmacy and Biochemistry, Genos Ltd.","award":["KK.01.2.2.03.0006"],"award-info":[{"award-number":["KK.01.2.2.03.0006"]}]},{"name":"Genetic Counselling Unit, University Hospital for Tumours, Sisters of Charity University Hospital Center","award":["KK.01.1.1.01.0010"],"award-info":[{"award-number":["KK.01.1.1.01.0010"]}]},{"name":"Genetic Counselling Unit, University Hospital for Tumours, Sisters of Charity University Hospital Center","award":["KK.01.2.2.03.0006"],"award-info":[{"award-number":["KK.01.2.2.03.0006"]}]},{"DOI":"10.13039\/501100000780","name":"centre of Research Excellence","doi-asserted-by":"publisher","award":["KK.01.1.1.01.0010"],"award-info":[{"award-number":["KK.01.1.1.01.0010"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"centre of Research Excellence","doi-asserted-by":"publisher","award":["KK.01.2.2.03.0006"],"award-info":[{"award-number":["KK.01.2.2.03.0006"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Structural and Investment Funds grant under the project called \u201cCEKOM: Croatian National Centre of Competence in Molecular Diagnostics\u201d","award":["KK.01.1.1.01.0010"],"award-info":[{"award-number":["KK.01.1.1.01.0010"]}]},{"name":"European Structural and Investment Funds grant under the project called \u201cCEKOM: Croatian National Centre of Competence in Molecular Diagnostics\u201d","award":["KK.01.2.2.03.0006"],"award-info":[{"award-number":["KK.01.2.2.03.0006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibodies"],"abstract":"<jats:p>This study examines the intricate relationship between protein glycosylation dynamics and therapeutic responses in Luminal A and Luminal B breast cancer subtypes, focusing on anastrozole and tamoxifen impacts. The present methods inadequately monitor and forecast patient reactions to these treatments, leaving individuals vulnerable to the potential adverse effects of these medications. This research investigated glycan structural changes by following patients for up to 9 months. The protocol involved a series of automated steps including IgG isolation, protein denaturation, glycan labelling, purification, and final analysis using capillary gel electrophoresis with laser-induced fluorescence. The results suggested the significant role of glycan modifications in breast cancer progression, revealing distinctive trends in how anastrozole and tamoxifen elicit varied responses. The findings indicate anastrozole\u2019s association with reduced sialylation and increased core fucosylation, while tamoxifen correlated with increased sialylation and decreased core fucosylation. These observations suggest potential immunomodulatory effects: anastrozole possibly reducing inflammation and tamoxifen impacting immune-mediated cytotoxicity. This study strongly emphasizes the importance of considering specific glycan traits to comprehend the dynamic mechanisms driving breast cancer progression and the effects of targeted therapies. The nuanced differences observed in glycan modifications between these two treatments underscore the necessity for further comprehensive research aimed at thoroughly evaluating the long-term implications and therapeutic efficacy for breast cancer patients.<\/jats:p>","DOI":"10.3390\/antib13010009","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T09:43:22Z","timestamp":1706780602000},"page":"9","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anastrozole and Tamoxifen Impact on IgG Glycome Composition Dynamics in Luminal A and Luminal B Breast Cancers"],"prefix":"10.3390","volume":"13","author":[{"given":"Borna","family":"Rap\u010dan","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kova\u010di\u0107a 1, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matko","family":"Fan\u010dovi\u0107","sequence":"additional","affiliation":[{"name":"Genos Ltd., Borongajska Cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tea","family":"Pribi\u0107","sequence":"additional","affiliation":[{"name":"Genos Ltd., Borongajska Cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Kirac","sequence":"additional","affiliation":[{"name":"Genetic Counselling Unit, University Hospital for Tumours, Sestre Milosrdnice University Hospital Center, Ilica 197, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihaela","family":"Ga\u0107e","sequence":"additional","affiliation":[{"name":"Genetic Counselling Unit, University Hospital for Tumours, Sestre Milosrdnice University Hospital Center, Ilica 197, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frano","family":"Vu\u010dkovi\u0107","sequence":"additional","affiliation":[{"name":"Genos Ltd., Borongajska Cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordan","family":"Lauc","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kova\u010di\u0107a 1, 10000 Zagreb, Croatia"},{"name":"Genos Ltd., Borongajska Cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Total synthesis of glycosylated proteins","volume":"Volume 362","author":"Brailsford","year":"2015","journal-title":"Protein Ligation and Total Synthesis I"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/glycob\/cww086","article-title":"Biological roles of glycans","volume":"27","author":"Varki","year":"2017","journal-title":"Glycobiology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.4049\/jimmunol.1502136","article-title":"The Emerging Importance of IgG Fab Glycosylation in Immunity","volume":"196","author":"Hafkenscheid","year":"2016","journal-title":"J. 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