{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:15:34Z","timestamp":1760112934400,"version":"build-2065373602"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"McKnight Brain Institute, the Center for Translational Research in Neurodegenerative Diseases at the University of Florida","doi-asserted-by":"publisher","award":["P30AG066506"],"award-info":[{"award-number":["P30AG066506"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibodies"],"abstract":"<jats:p>Amyloid-\u03b2 (A\u03b2) deposition throughout the neuroaxis is a classical hallmark of several neurodegenerative diseases, most notably Alzheimer\u2019s disease (AD). A\u03b2 peptides of varied length and diverse structural conformations are deposited within the parenchyma and vasculature in the brains of individuals with AD. Neuropathologically, A\u03b2 pathology can be assessed using antibodies to label and characterize their features, which in turn leads to a more extensive understanding of the pathological process. In the present study, we generated a novel monoclonal antibody, which we found to be specific for the N-terminal region of A\u03b2. This antibody reacted to amyloid precursor protein expressed in cultured cells and labels A\u03b2 plaques and cerebral amyloid angiopathy in brain tissue from a mouse model of amyloidosis as well as post-mortem brain tissue from patients diagnosed with AD. This highly specific novel antibody will serve as a unique tool for future studies investigating A\u03b2 deposition in novel mouse models and cross-sectional studies using post-mortem human tissue.<\/jats:p>","DOI":"10.3390\/antib13030068","type":"journal-article","created":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T06:25:24Z","timestamp":1723271124000},"page":"68","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Monoclonal Antibody Specific toward Amyloid-\u03b2 Binds to a Unique Epitope within the N-Terminal Region"],"prefix":"10.3390","volume":"13","author":[{"given":"Giavanna","family":"Paterno","sequence":"first","affiliation":[{"name":"Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda D.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA"},{"name":"Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brach M.","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly-Marie M.","family":"Gorion","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA"},{"name":"Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5633-2149","authenticated-orcid":false,"given":"Stefan","family":"Prokop","sequence":"additional","affiliation":[{"name":"Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Department of Pathology, College of Medicine, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd E.","family":"Golde","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA"},{"name":"Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, GA 30322, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit I.","family":"Giasson","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, FL 32610, USA"},{"name":"McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610, 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