{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:22:58Z","timestamp":1790392978383,"version":"4.1.0"},"reference-count":65,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1040924"],"award-info":[{"award-number":["1040924"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The main objective of this study was to characterize using whole-genome sequencing analysis, a new variant of the qnrB gene (qnrB89) carried by a fluoroquinolone-susceptible bacterium isolated from mucus of farmed Salmo salar fingerling in Chile. Citrobacter gillenii FP75 was identified by using biochemical tests and 16S ribosomal gene analysis. Nucleotide and amino acid sequences of the qnrB89 gene exhibited an identity to qnrB of 81.24% and 91.59%, respectively. The genetic environment of qnrB89 was characterized by the upstream location of a sequence encoding for a protein containing a heavy metal-binding domain and a gene encoding for a N-acetylmuramoyl-L-alanine amidase protein, whereas downstream to qnrB89 gene were detected the csp and cspG genes, encoding cold-shock proteins. The qnrB89 gene was located on a large chromosomal contig of the FP75 genome and was not associated with the 10-kb plasmid and class 1 integron harbored by the FP75 strain. This study reports for the first time the carriage of a qnrB gene by the C. gillenii species, and its detection in a bacterial strain isolated from farmed salmon in Chile.<\/jats:p>","DOI":"10.3390\/antibiotics10030236","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T04:36:24Z","timestamp":1614314184000},"page":"236","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of a Novel Variant of the Quinolone-Resistance Gene qnrB (qnrB89) Carried by a Multi-Drug Resistant Citrobacter gillenii Strain Isolated from Farmed Salmon in Chile"],"prefix":"10.3390","volume":"10","author":[{"given":"Christopher","family":"Concha","sequence":"first","affiliation":[{"name":"Laboratorio de Patobiolog\u00eda Acu\u00e1tica, Departamento de Acuicultura, Universidad Cat\u00f3lica del Norte, 1780000 Coquimbo, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio D.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laboratorio de Patobiolog\u00eda Acu\u00e1tica, Departamento de Acuicultura, Universidad Cat\u00f3lica del Norte, 1780000 Coquimbo, Chile"},{"name":"Centro AquaPac\u00edfico, 1780000 Coquimbo, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Rojas","sequence":"additional","affiliation":[{"name":"Laboratorio de Patobiolog\u00eda Acu\u00e1tica, Departamento de Acuicultura, Universidad Cat\u00f3lica del Norte, 1780000 Coquimbo, Chile"},{"name":"Centro AquaPac\u00edfico, 1780000 Coquimbo, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix A.","family":"Godoy","sequence":"additional","affiliation":[{"name":"Centro i~mar, Universidad de Los Lagos, 5480000 Puerto Montt, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9614-2393","authenticated-orcid":false,"given":"Jaime","family":"Romero","sequence":"additional","affiliation":[{"name":"Centro AquaPac\u00edfico, 1780000 Coquimbo, Chile"},{"name":"Laboratorio de Biotecnolog\u00eda, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos (INTA), Universidad de Chile, Macul, 7810000 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/jfd.12211","article-title":"Piscirickettsiosis and Piscirickettsia salmonis in fish: A review","volume":"37","author":"Rozas","year":"2014","journal-title":"J. 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