{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:07:59Z","timestamp":1773814079037,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2019R1F1A1061747"],"award-info":[{"award-number":["NRF-2019R1F1A1061747"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ013985022020"],"award-info":[{"award-number":["PJ013985022020"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The emergence of extended-spectrum cephalosporin (ESC)-resistant Gram-negative bacteria is of great concern in both human and veterinary medicine. The aim of this study was to investigate ESC-resistant bacterial isolates from companion animals in South Korea between 2017 and 2019. Isolates with ESC resistance genes, which were identified by PCR, were assessed for genetic relatedness by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). In total, 91 ESC-resistant Escherichia coli, Klebsiella spp., Serratia spp., and Enterobacter cloacae isolates harbored the blaTEM gene. Among other ESC resistance genes, blaCTX-M-15, blaCIT, and blaCTX-M-55 were predominantly detected in E. coli isolates, whereas blaSHV and blaDHA were more frequently detected in Klebsiella pneumoniae isolates. In addition, all blaEBC-positive isolates were classified as E. cloacae. From the MLST results, blaCTX-M-9-carrying ST131, blaCIT-carrying ST405, and blaCTX-M-1-carrying ST3285 strains were dominant among E. coli isolates. ST273 and ST275 strains harboring blaSHV were frequently detected in K. pneumoniae isolates. Various sequence types were obtained in E. cloacae and Klebsiella oxytoca isolates. All isolates demonstrated unique PFGE profiles (&lt;57\u201398% similarity) and were unlikely to be derived from a single clone. The present study reveals the presence and wide genetic distribution of ESC-resistant bacterial species in South Korean companion animals.<\/jats:p>","DOI":"10.3390\/antibiotics10030249","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T05:10:16Z","timestamp":1614748216000},"page":"249","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of Extended-Spectrum \u03b2-Lactamase-Producing and AmpC \u03b2-Lactamase-Producing Enterobacterales Isolated from Companion Animals in Korea"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9528-7790","authenticated-orcid":false,"given":"Se Ra","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"},{"name":"BK21 PLUS Creative Veterinary Research Center, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Mi","family":"Noh","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"},{"name":"BK21 PLUS Creative Veterinary Research Center, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Kyung","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sook","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Kyung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1244-201X","authenticated-orcid":false,"given":"Dong Chan","family":"Moon","sequence":"additional","affiliation":[{"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suk-Kyung","family":"Lim","sequence":"additional","affiliation":[{"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong Ho","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Preventive Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6177-0373","authenticated-orcid":false,"given":"Kun Taek","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Inje University, Gimhae 50834, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,3]]},"reference":[{"key":"ref_1","first-page":"9519718","article-title":"The growing genetic and functional diversity of extended spectrum \u03b2-lactamases","volume":"2018","author":"Ali","year":"2018","journal-title":"BioMed Res. 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