{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T16:41:36Z","timestamp":1769877696247,"version":"3.49.0"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T00:00:00Z","timestamp":1617667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["75010330"],"award-info":[{"award-number":["75010330"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Staphylococcus aureus is one of the major causes of bloodstream infections. The aim of our study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from blood of patients hospitalized in the Czech Republic between 2016 and 2018. All MRSA strains were tested for antibiotic susceptibility, analyzed by spa typing and clustered using a Based Upon Repeat Pattern (BURP) algorithm. The representative isolates of the four most common spa types and representative isolates of all spa clonal complexes were further typed by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. The majority of MRSA strains were resistant to ciprofloxacin (94%), erythromycin (95.5%) and clindamycin (95.6%). Among the 618 strains analyzed, 52 different spa types were detected. BURP analysis divided them into six different clusters. The most common spa types were t003, t586, t014 and t002, all belonging to the CC5 (clonal complex). CC5 was the most abundant MLST CC of our study, comprising of 91.7% (n = 565) of spa-typeable isolates. Other CCs present in our study were CC398, CC22, CC8, CC45 and CC97. To our knowledge, this is the biggest nationwide study aimed at typing MRSA blood isolates from the Czech Republic.<\/jats:p>","DOI":"10.3390\/antibiotics10040395","type":"journal-article","created":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T10:34:12Z","timestamp":1617705252000},"page":"395","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1077-7916","authenticated-orcid":false,"given":"Katarina","family":"Pomorska","sequence":"first","affiliation":[{"name":"Centre for Epidemiology and Microbiology, National Reference Laboratory for Antibiotics, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Jakubu","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Microbiology, National Reference Laboratory for Antibiotics, National Institute of Public Health, 10000 Prague, Czech Republic"},{"name":"Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, 53002 Hradec Kralove, Czech Republic"},{"name":"Department of Microbiology, 3rd Faculty of Medicine Charles University, University Hospital Kralovske Vinohrady, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Malisova","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Microbiology, National Reference Laboratory for Antibiotics, National Institute of Public Health, 10000 Prague, Czech Republic"},{"name":"Department of Microbiology, 3rd Faculty of Medicine Charles University, University Hospital Kralovske Vinohrady, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Fridrichova","sequence":"additional","affiliation":[{"name":"Department of Microbiology, 3rd Faculty of Medicine Charles University, University Hospital Kralovske Vinohrady, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2238-1599","authenticated-orcid":false,"given":"Martin","family":"Musilek","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Microbiology, National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3868-0545","authenticated-orcid":false,"given":"Helena","family":"Zemlickova","sequence":"additional","affiliation":[{"name":"Centre for Epidemiology and Microbiology, National Reference Laboratory for Antibiotics, National Institute of Public Health, 10000 Prague, Czech Republic"},{"name":"Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, 53002 Hradec Kralove, Czech Republic"},{"name":"Department of Microbiology, 3rd Faculty of Medicine Charles University, University Hospital Kralovske Vinohrady, National Institute of Public Health, 10000 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/CMR.00134-14","article-title":"Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management","volume":"28","author":"Tong","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","unstructured":"European Centre for Disease Prevention and Control (2017). Surveillance of Antimicrobial Resistance in Europe 2016, ECDC. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net)."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"20987","DOI":"10.2807\/1560-7917.ES2014.19.49.20987","article-title":"The dynamic changes of dominant clones of Staphylococcus aureus causing bloodstream infections in the European region: Results of a second structured survey","volume":"19","author":"Grundmann","year":"2014","journal-title":"Euro Surveill."},{"key":"ref_4","unstructured":"(2020, April 10). Surveillance Atlas of Infectious Diseases. Available online: http:\/\/atlas.ecdc.europa.eu\/public\/index.aspx."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2798","DOI":"10.1128\/JCM.37.9.2798-2803.1999","article-title":"Methicillin-resistant Staphylococcus aureus clonal types in the Czech Republic","volume":"37","author":"Melter","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4998","DOI":"10.1128\/JCM.41.11.4998-5005.2003","article-title":"Update on the major clonal types of methicillin-resistant Staphylococcus aureus in the Czech Republic","volume":"41","author":"Melter","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","first-page":"E060803-6","article-title":"Emergence of EMRSA-15 clone in hospitals throughout the Czech Republic","volume":"11","author":"Melter","year":"2006","journal-title":"Euro Surveill."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e10000215","DOI":"10.1371\/journal.pmed.1000215","article-title":"Geographic distribution of Staphylococcus aureus causing invasive infections in Europe: A molecular-epidemiological analysis","volume":"7","author":"Grundmann","year":"2010","journal-title":"PLoS Med."},{"key":"ref_9","unstructured":"Pomorska, K., Jakubu, V., Musilek, M., and Zemlickova, H. (2019, January 13\u201316). Spa typing of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from blood from patients hospitalized in the Czech Republic: A comparative study after ten years. Proceedings of the 29th Congress of Clinical Microbiology & Infectious Diseases (ECCMID), Amsterdam, The Netherlands. Poster no. P2716."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/jac\/dkaa404","article-title":"The molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in the Czech Republic","volume":"76","author":"Tkadlec","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s12223-020-00782-9","article-title":"Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from bloodstream cultures at University Hospital in the Czech Republic","volume":"65","author":"Neradova","year":"2020","journal-title":"Folia Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Stock, N.K., Petr\u00e1\u0161, P., Melter, O., Kapounov\u00e1, G., Vopalkov\u00e1, P., Kubele, J., Vani\u0161, V., Tkadlec, J., Buk\u00e1\u010dkov\u00e1, E., and Machov\u00e1, I. (2016). Importance of multifaceted approaches in infection control: A practical experience from an outbreak investigation. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0157981"},{"key":"ref_14","unstructured":"(2020, December 18). Ridom SpaServer\u2014Frequencies. Available online: https:\/\/spa.ridom.de\/frequencies.shtml."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2533","DOI":"10.1128\/JCM.00420-06","article-title":"Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing","volume":"44","author":"Strommenger","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e00444-16","DOI":"10.1128\/mBio.00444-16","article-title":"Whole-genome sequencing for routine pathogen surveillance in public health: A Population snapshot of invasive Staphylococcus aureus in Europe","volume":"7","author":"Aanensen","year":"2016","journal-title":"MBio"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"N\u00fcbel, U., Dordel, J., Kurt, K., Strommenger, B., Westh, H., Shukla, S.K., Zemlickov\u00e1, H., Leblois, R., Wirth, T., and Jombart, T. (2010). A timescale for evolution, population expansion, and spatial spread of an emerging clone of methicillin-resistant Staphylococcus aureus. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1000855"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1086\/515610","article-title":"Molecular epidemiology of methicillin-resistant Staphylococcus aureus in 12 New York hospitals","volume":"178","author":"Roberts","year":"1998","journal-title":"J. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pardos de la Gandara, M., Curry, M., Berger, J., Burstein, D., Della-Latta, P., Kopetz, V., Quale, J., Spitzer, E., Tan, R., and Urban, C. (2016). MRSA causing infections in hospitals in greater metropolitan New York: Major shift in the dominant clonal type between 1996 and 2014. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156924"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1089\/mdr.2000.6.253","article-title":"Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan","volume":"6","author":"Lencastre","year":"2000","journal-title":"Microb. Drug. Resist."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1469-0691.2007.01794.x","article-title":"Replacement of methicillin-resistant Staphylococcus aureus clones in Hungary over time: A 10-year surveillance study","volume":"13","author":"Grundmann","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2912","DOI":"10.1128\/JCM.00692-08","article-title":"Changing patterns in frequency of recovery of five methicillin-resistant Staphylococcus aureus clones in Portuguese hospitals: Surveillance over a 16-year period","volume":"46","author":"Correia","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"fnw137","DOI":"10.1093\/femsle\/fnw137","article-title":"Increase of genetic diversity and clonal replacement of epidemic methicillin-resistant Staphylococcus aureus strains in South-East Austria","volume":"363","author":"Zarfel","year":"2016","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Monecke, S., Coombs, G., Shore, A.C., Coleman, D.C., Akpaka, P., Borg, M., Chow, H., Ip, M., Jatzwauk, L., and Jonas, D. (2011). A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017936"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.3389\/fmicb.2017.02493","article-title":"Molecular epidemiology of methicillin-resistant Staphylococcus aureus in horses, cats, and dogs over a 5-year period in France","volume":"8","author":"Haenni","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1089\/fpd.2018.2511","article-title":"Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep, and goats","volume":"16","author":"Tegegne","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.apjtm.2017.08.013","article-title":"Diversity of livestock associated methicillin-resistant Staphylococcus aureus","volume":"10","author":"Tegegne","year":"2017","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Neradova, K., Jakubu, V., Pomorska, K., and Zemlickova, H. (2020). Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-019-2223-z"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8051","DOI":"10.2807\/ese.13.09.08051-en","article-title":"First outbreak of methicillin-resistant Staphylococcus aureus ST398 in a Dutch hospital, June 2007","volume":"13","author":"Wulf","year":"2008","journal-title":"Euro Surveill."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1128\/JCM.39.4.1540-1548.2001","article-title":"Identification and characterization of phage variants of a strain of epidemic methicillin-resistant Staphylococcus aureus (EMRSA-15)","volume":"39","author":"Murchan","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1089\/mdr.2013.0149","article-title":"Massive dissemination of methicillin resistant Staphylococcus aureus in bloodstream infections in a high MRSA prevalence country: Establishment and diversification of EMRSA-15","volume":"19","author":"Faria","year":"2013","journal-title":"Microb. Drug Resist."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/jac\/dkv298","article-title":"Molecular epidemiology of MRSA in 13 ICUs from eight European countries","volume":"71","author":"Hetem","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e02198-20","DOI":"10.1128\/JCM.02198-20","article-title":"Global epidemiology and evolutionary history of Staphylococcus aureus ST45","volume":"59","author":"Effelsberg","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1017\/S0950268809002015","article-title":"Carriage of methicillin-resistant Staphylococcus aureus in veterinary personnel","volume":"137","author":"Zemlickova","year":"2009","journal-title":"Epidemiol. Infect."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1078\/1438-4221-00116","article-title":"Subtyping of MRSA isolates belonging to a widely disseminated clonal group by polymorphism of the dru sequences in mec-associated DNA","volume":"291","author":"Witte","year":"2001","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1099\/jmm.0.028381-0","article-title":"Detection and characterization of an ST97-SCCmec-V community-associated meticillin-resistant Staphylococcus aureus clone in a neonatal intensive care unit and special care baby unit","volume":"60","author":"Udo","year":"2011","journal-title":"J. Med. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.3389\/fmicb.2018.01494","article-title":"A sporadic four-year hospital outbreak of a ST97-IVa MRSA with half of the patients first identified in the community","volume":"9","author":"Rubin","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/j.cmi.2019.03.024","article-title":"Microbiological diagnostics of bloodstream infections in Europe-an ESGBIES survey","volume":"25","author":"Idelevich","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_39","unstructured":"European Centre for Disease Prevention and Control (2019). Surveillance of Antimicrobial Resistance in Europe 2018, ECDC. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net)."},{"key":"ref_40","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2019). Breakpoint Tables for Interpretation of MICs and Zone Diameters, EUCAST. Version 9.0."},{"key":"ref_41","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2018). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [28th ed.]. CLSI Supplement M100."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1128\/AAC.46.7.2155-2161.2002","article-title":"Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus","volume":"46","author":"Oliveira","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1469-0691.2011.03715.x","article-title":"Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251)","volume":"18","author":"Stegger","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_44","unstructured":"(2020, December 18). Ridom Bioinformatics DNA Sequencing of the Spa Gene. Available online: https:\/\/www.ridom.de\/doc\/Ridom_spa_sequencing.pdf."},{"key":"ref_45","unstructured":"(2020, December 18). Ridom StaphType User Guide, Version 2. Available online: https:\/\/www.ridom.de\/doc\/Ridom_StaphType_user_guide.pdf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","article-title":"Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus","volume":"38","author":"Enright","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1128\/AAC.00275-07","article-title":"Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus","volume":"51","author":"Oliveira","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1128\/JCM.43.10.5026-5033.2005","article-title":"Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus","volume":"43","author":"Zhang","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s10156-011-0223-4","article-title":"Dissemination of multiple MRSA clones among community-associated methicillin-resistant Staphylococcus aureus infections from Japanese children with impetigo","volume":"17","author":"Hisata","year":"2011","journal-title":"J. Infect. Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4719","DOI":"10.1128\/JCM.43.9.4719-4730.2005","article-title":"Successful multiresistant community-associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan, that carries either the novel staphylococcal chromosome cassette mec (SCCmec) type VT or SCCmec type IV","volume":"43","author":"Ereshefsky","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1128\/AAC.01001-07","article-title":"Novel characteristics of community-acquired methicillin-resistant Staphylococcus aureus strains belonging to multilocus sequence type 59 in Taiwan","volume":"52","author":"Takano","year":"2008","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/10\/4\/395\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:11:24Z","timestamp":1760364684000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/10\/4\/395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,6]]},"references-count":51,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["antibiotics10040395"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics10040395","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,6]]}}}