{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:29:05Z","timestamp":1760236145218,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014261","name":"National Institute for Health Care Management Foundation","doi-asserted-by":"publisher","award":["NIH-R56 AI118756"],"award-info":[{"award-number":["NIH-R56 AI118756"]}],"id":[{"id":"10.13039\/100014261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Staphylococcus pseudintermedius is an important pathogen responsible for infections in dogs and in humans. The emergence and dissemination of methicillin-resistant S. pseudintermedius (MRSP) and the multidrug resistance frequently seen in this species make difficult the treatment of these pathogens. The cefoxitin disk is widely used as a marker of methicillin resistance mediated by the mecA gene in Staphylococcus aureus and other staphylococcal species; however, it is not useful to detect \u03b2-lactam resistance of MRSP in clinical microbiology laboratories. The purpose of this study was to elucidate the molecular bases of the dissociated phenotype between oxacillin and cefoxitin antibiotics. By using a combinatorial approach that included the Penicillin-Binding Proteins\u2019 (PBP) profile, their affinity for different \u03b2-lactam antibiotics and the analyses of PBPs\u2019 sequence, we provide evidence that PBP4 showed still affinity for its target cefoxitin, impairing its phenotypic resistant detection in MRSP. Together, these findings provide evidence that S. pseudintermedius PBP4 is directly associated with the dissociated oxacillin and cefoxitin phenotype.<\/jats:p>","DOI":"10.3390\/antibiotics10111299","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T23:49:52Z","timestamp":1635292192000},"page":"1299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Staphylococcus pseudintermedius\u2019s PBP4 Is Directly Associated with the Dissociated Oxacillin and Cefoxitin Phenotype"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1551-7208","authenticated-orcid":false,"given":"Paula","family":"Gagetti","sequence":"first","affiliation":[{"name":"Servicio Antimicrobianos, INEI-ANLIS, Buenos Aires 1281, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto R.","family":"Rosato","sequence":"additional","affiliation":[{"name":"Houston Methodist Cancer Center, Houston Methodist Research Institute, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6115-6955","authenticated-orcid":false,"given":"Adriana E.","family":"Rosato","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Microbiology Diagnostics-Research, Riverside University Health System, Moreno Valley, CA 92555, USA"},{"name":"School of Medicine, University of California Riverside, 900 University Avenue Riverside, Riverside, CA 92521, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1128\/JCM.01288-15","article-title":"Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands","volume":"54","author":"Duim","year":"2016","journal-title":"J. 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