{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:38:43Z","timestamp":1793216323492,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural  Science Foundation of China","award":["no. 31972599"],"award-info":[{"award-number":["no. 31972599"]}]},{"name":"the Key Research and Development Program of Sichuan Province","award":["2020YFN0147"],"award-info":[{"award-number":["2020YFN0147"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: To examine the effect of \u03b2-glucan (BGL) supplementation on growth performance and intestinal epithelium functions in weaned pigs upon Enterotoxigenic Escherichia coli (ETEC) challenge. Methods: Thirty-two weaned pigs (Duroc \u00d7 Landrace \u00d7 Yorkshire) were assigned into four groups. Pigs fed with a basal diet or basal diet containing 500 mg\/kg BGL were orally infused with ETEC or culture medium. Results: Results showed BGL tended to increase the average daily gain (ADG) in ETEC-challenged pigs (0.05 &lt; p &lt; 0.1). Dietary BGL supplementation had no significant influence on nutrient digestibility (p &gt; 0.05). However, BGL improved the serum concentrations of immunoglobulin (Ig) A and IgG, and was beneficial to relieve the increasement of the concentrations of inflammatory cytokines such as the TNF-\u03b1 and IL-6 upon ETEC-challenge (p &lt; 0.05). Interestingly, BGL significantly increased the duodenal, jejunal and ileal villus height, and increased the jejunal ratio of villus height to crypt depth (V\/C) upon ETEC challenge (p &lt; 0.05). BGL also increased the activities of mucosal, sucrase and maltase in the ETEC-challenged pigs (p &lt; 0.05). Moreover, BGL elevated the abundance of Lactobacillus and the concentration of propanoic acid in colon in the ETEC-challenged pigs (p &lt; 0.05). Importantly, BGL elevated the expression levels of zonula occludins-1 (ZO-1) and mucin-2 (MUC-2) in the small intestinal mucosa upon ETEC challenge (p &lt; 0.05). BGL also upregulated the expressions of functional genes such as the claudin-1, cationic amino acid transporter-1 (CAT-1), LAT-1, L amino acid transporter-1 (LAT1), fatty acid transport proteins (FATP1), FATP4, and sodium\/glucose cotransporter-1 (SGLT-1) in the duodenum, and the occludin-1 and CAT-1 in the jejunum upon ETEC challenge (p &lt; 0.05). Conclusions: These results suggested that BGL can attenuate intestinal damage in weaned pigs upon ETEC challenge, which was connected with the suppressed secretion of inflammatory cytokines and enhanced serum immunoglobulins, as well as improved intestinal epithelium functions and microbiota.<\/jats:p>","DOI":"10.3390\/antibiotics11040519","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T21:33:42Z","timestamp":1649885622000},"page":"519","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Effect of \u03b2-Glucan Supplementation on Growth Performance and Intestinal Epithelium Functions in Weaned Pigs Challenged by Enterotoxigenic Escherichia coli"],"prefix":"10.3390","volume":"11","author":[{"given":"Yuankang","family":"Zhou","sequence":"first","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6605-6742","authenticated-orcid":false,"given":"Bing","family":"Yu","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yu","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5092-9297","authenticated-orcid":false,"given":"Zhiqing","family":"Huang","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-7747","authenticated-orcid":false,"given":"Xiangbing","family":"Mao","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiu","family":"Luo","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yan","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1963-9766","authenticated-orcid":false,"given":"Jun","family":"He","sequence":"additional","affiliation":[{"name":"Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"Key Laboratory of Animal Disease-Resistance Nutrition, Chengdu 625014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/2049-1891-4-19","article-title":"The biological stress of early weaned piglets","volume":"4","author":"Campbell","year":"2013","journal-title":"J. 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