{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:01:46Z","timestamp":1792623706364,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfeicoamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.318820\/2019-00)."],"award-info":[{"award-number":["88887.318820\/2019-00)."]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>C. albicans and C. parapsilosis are biofilm-forming yeasts responsible for bloodstream infections that can cause death. Synthetic antimicrobial peptides (SAMPs) are considered to be new weapons to combat these infections, alone or combined with drugs. Here, two SAMPs, called Mo-CBP3-PepI and Mo-CBP3-PepIII, were tested alone or combined with nystatin (NYS) and itraconazole (ITR) against C. albicans and C. parapsilosis biofilms. Furthermore, the mechanism of antibiofilm activity was evaluated by fluorescence and scanning electron microscopies. When combined with SAMPs, the results revealed a 2- to 4-fold improvement of NYS and ITR antibiofilm activity. Microscopic analyses showed cell membrane and wall damage and ROS overproduction, which caused leakage of internal content and cell death. Taken together, these results suggest the potential of Mo-CBP3-PepI and Mo-CBP3-PepIII as new drugs and adjuvants to increase the activity of conventional drugs for the treatment of clinical infections caused by C. albicans and C. parapsilosis.<\/jats:p>","DOI":"10.3390\/antibiotics11050553","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T05:07:22Z","timestamp":1650604042000},"page":"553","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Synergistic Antifungal Activity of Synthetic Peptides and Antifungal Drugs against Candida albicans and C. parapsilosis Biofilms"],"prefix":"10.3390","volume":"11","author":[{"given":"Leandro P.","family":"Bezerra","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleverson D. T.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayrles F. B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackson L.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton A. S.","family":"Neto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael G. G.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aura L. C.","family":"Parra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, S\u00e3o Paulo P.O. Box 05508-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-1140","authenticated-orcid":false,"given":"Jose T. A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe P.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Cear\u00e1, Rua Coronel, Nunes de Melo 100, Caixa, Fortaleza 60430-275, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2524-4434","authenticated-orcid":false,"given":"Pedro F. N.","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451, CE, Brazil"},{"name":"Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Cear\u00e1, Rua Coronel, Nunes de Melo 100, Caixa, Fortaleza 60430-275, CE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"119647","DOI":"10.1016\/j.lfs.2021.119647","article-title":"Synthetic antimicrobial peptides: Characteristics, design, and potential as alternative molecules to overcome microbial resistance","volume":"278","author":"Lima","year":"2021","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.ijmm.2017.09.016","article-title":"Biofilms: Survival and defense strategy for pathogens","volume":"307","author":"Kumar","year":"2017","journal-title":"Int. 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